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지윤의 ( Ji Yun Ui ),이미정 ( Lee Mi Jeong ),김효정 ( Kim Hyo Jeong ),이규석 ( Lee Gyu Seog ),이선 ( Lee Seon ),송호경 ( Song Ho Gyeong ) 한국환경생물학회 2003 환경생물 : 환경생물학회지 Vol.21 No.3
본 연구는 충남 금산군에 위치한 진악산 산림식생을 대상으로 식물사회학적 방법으로 산림군락을 분류하고 방형구법으로 임분 특성을 분석하였다. 진악산의 26개 조사구를 분석한 결과, 산림군락은 신갈나무군락, 굴참나무군락, 갈참나무군락 및 소나무군락으로 구분되었으며, 피도율은 교목층이 79.4%, 아교목층이 27.6%, 관목층이 37.0%, 초본층이 31.1%의 순으로 나타났다. DBH 2㎝ 이상의 수목을 대상으로 중요치를 분석한 결과는 신갈나무가 45.51, 소나무가 44.17, 굴참나무가 27.56, 졸참나무가 26.78, 갈참나무가 20.81, 벚나무가 15.58 등의 순으로 나타났으며, 흉고직경급을 분석한 결과 신갈나무, 굴참나무, 갈참나무, 소나무는 5㎝ 이하의 어린 개체와 15㎝ 이상의 큰 개체는 적고 5~15㎝의 중간 개체가 많아 이들이 당분간 우점할 것으로 판단된다. This study was carried out to analyze forest vegetation of Jinaksan in Geumsan, Chungnam Province. Employing the releve method of Braun-Blanquet, 26 plots were sampled in forest of Jinaksan. The communities were classified into Quercus mongolica, Quercus uariabilis, Quercus aliena, and Pinus densiflora communities. Coverage rate was 79.4% in tree layer, 27.6% in subtree layer, 37.0% in shrub layer, 31.1% in herb layer, respectively. The importance values were 45.51 in Q. mongolica, 44.17 in P. densiflora, 26.56 in Q. uariabilis, 26.78 in Q. serrata, 20.81 in Q. aliena, and 15.58 infPrunus serrulata var. spontanea, respectively. Most of the DBH in the Q. mongolica, Q. variabilis, Q. aliena, and P. densiflora was between 5 ㎝ and 15 ㎝. Therefore, Q. mon-golica, Q. variabilis, and Q. aliena will be dominant species in the study area for several decades.
외대잔대(Adenophora raoemosa J.Lee & S.Lee)개체군의 classification과 ordination 분석
지윤의 ( Yun Ui Ji ),문병철 ( Byeong Cheol Moon ),이야영 ( A Yeong Lee ),윤태숙 ( Tae Sook Yoon ),송호경 ( Ho Kyung Song ),추병길 ( Byung Kil Choo ),김호경 ( Ho Kyoung Kim ) 한국환경보건기술학회(구 한국환경복원녹화기술학회) 2009 한국환경복원기술학회지 Vol.12 No.6
This study was carried out to investigate vegetation structure and soil properties of Adenophora racemosa population distributed in Jeombongsan, Seoraksan, Odaesan and Dutasan in Gangwon-do, Korea. From August 2007 until September 2009, 2 m × 2 m quadrate was established in native area of Adenophora racemosa in order to record a dominants and coverage, and soil factors at 22 sites. It was found that the altitude in the distributed areas for Angelica gigas population was 800 m or more. Adenophora racemosa population was classified into Rhododendron mucronulatum dominant population, Geranium var. hirsutum dominant population, Carex siderosticta dominant population and Aruncus dioicus var. kamtschaticus dominant population. In the site of study, soil pH, electrical conductivity, soil organic matter, available phosphorous, and exchangeable potassimn, exchangeable calcimn, exchangeable magnesium, exchangeable sodium concentration and total nitrogen were ranged from 5.0~7.1, 0.06~0.65dS/m, 0.96~8.94%, 12.3~32.8mg/kg, 0.12~0.89cmol+/kg, 0.34~10.08cmol+/kg, 0.1~l.4cmol+/kg, 0.02~0.29cmol+/kg and 0~8.4% respectively. According to the results of DCCA, Adenophora racemosa population were distributed in the high available phosphorous and exchangeable potassium, and Rhododendron mucronulatum dominant population was situated on low available potassium and high slope degree, Geranium var. hirsutum dominant population was high altitude, electrical conductivity and exchange magnesium, and Carex siderosticta dominant population and Aruncus dioicus var. kamtschaticus were distributed on high total nitrogen, organic matter, available phosphorous.
이순혁,지윤서,최상규,이레나,Lee, Soonhyouk,Ji, Yun-Seo,Choi, Sang Gyu,Lee, Rena 한국의학물리학회 2014 의학물리 Vol.25 No.4
The purpose of this study is to develope a control system for a small X-ray tube generator and investigate control methods for the X-ray generator. The small X-ray tube was developed for electronic brachytherapy, and thus, the new control method should be investigated, if the small X-ray tube is used for the imaging system. The Axxent S700 X-ray tube and the XF060NZZ485 high voltage generator were used to compose a X-ray imaging system and control board was developed by using AT90CAN128 MCU. The two control methods were investigated after tube voltage was set to 50 kV, one was filament current control method and the other was beam current control method. The former was subdivided into two methods according to the filament heating time, the 5 and the 10 seconds respectively. In the filament current method, the beam current did not rise up to the desired value, if the filament current had not been maintained for at least 10 seconds. The onset filament currents to generate beam current were varied from 1,300 to 1,350 mA and over 5 seconds were needed in order to reach the desired tube current value after beam current was generated. However, in the tube current control method, the beam current reached to the desired value without any time delay with the filament current of 1,500 mA. In this study, we found that the beam current control method was appropriate for the use of small X-ray tube developed for brachytherapy in the X-ray imaging system. 본 연구에서는 미국 Xoft 사에서 근접치료 장치(elecronic brachytherapy)를 목적으로 개발된 소형 X-선 튜브를 치과용 영상장비로 사용함에 있어서 고전압 발생장치의 제어용 장치를 개발하고 영상용 제어 방식으로 최적화하고자 한다. 연구에서 사용된 X-선 튜브와 고전압 발생 장치는 각각 Axxent S700과 XF060NZZ485를 사용하였고, AT90CAN128 MCU를 사용하여 제어보드를 제작하였다. 관전압을 50 kV로 고정한 후에 제어 방식에 따라서 필라멘트 전류 제어 방식과 관전류제어 방식으로 나누었다. 필라멘트 제어 방식은 다시 필라멘트 가열 시간에 따라서 5초와 10초의 두 가지로 나누어 실험하였다. 필라멘트 전류 제어 방식에서는 필라멘트 예열 시간이 10초 이상이 되지 않으면 설정된 관전류 값에 도달하기 어려웠고, 관전류가 발생하는 필라멘트 전류도 1,300~1,350 mA로 가변적이었으며, 관전류가 발생된 이후에도 설정된 목표 값에 도달하기 위해서는 약 5초 이상의 시간이 소요되었다. 하지만 관전류 제어 방식에서는 관전류가 설정된 목표 값에 시간 지연 없이 즉각적으로 도달하였으며 그 때의 필라멘트 전류 값은 1,500 mA였다. 본 연구에서는 소형의 X-선 튜브에 고전압을 안정적으로 공급하는 제어 장치를 개발하였고, 방사선 영상장비로 사용함에 있어서는 관전류 제어 방식이 적합함을 보였다.
HPLC-PDA를 이용한 반하, 호장남성, 수반하의 분류 및 함량분석
조지은 ( Ji Eun Jo ),이아영 ( A Yeong Lee ),김효선 ( Hyo Seon Kim ),문병철 ( Byeong Cheol Moon ),최고야 ( Go Ya Choi ),지윤의 ( Yun Ui Ji ),김호경 ( Ho Kyoung Kim ) 대한본초학회 2013 大韓本草學會誌 Vol.28 No.5
Objectives: A quantitative method using high performance liquid chromatography with a photodiode array detector(HPLC-PDA) was established for the quantitative analysis of the four main compound and pattern analysis to classification Piiellia ternate, P. pedatisecta and Typhonium flagelliforme. Methods: The analytical procedure for the determination of P. ternata, together with the known main compounds uracil, uridine, guanosine and adenosine was established. Optimum HPLC-PDA separation of these P. ternata was possible on Luna C18(2) column material, using water and acetonitrile as mobile phase. The method was validated according to regulatory guidelines. In addition, this assay method were analyzed for the content of four main compound in P. ternata, P. pedatisecta and T. flagelliforme and by data obtained from the HPLC-PDA analysis was performed principal component analysis(PCA). Results: Validation results indicated that the HPLC method is well suited for the determination of the roots of P. ternata with a good linearity (r2> 0.999), precision and recovery rates. Analysis of HPLC-PDA, the average content of uracil, uridine, guanosine and adenosine was significantly higher in P. ternate>P. pedatisecta> T. flagelliforme order. The application of PCA to main compound data by HPLC-PDA permitted the effective discrimination among the three species. Conclusions: Analysis of both HPLC-PDA and PCA confirmed the fact that four main compound and pattern profiles of P. ternata, P. pedatisecta and T. flagelliforme were different from each other.
이순혁,지윤서,이레나,Lee, Soonhyouk,Ji, Yun-Seo,Lee, Rena 한국의학물리학회 2013 의학물리 Vol.24 No.3
광 음향 영상 장치는 최근 들어 연구와 개발이 활발히 진행 중이며 암을 조기 진단할 수 있는 장치로서의 가능성을 보이고 있다. 본 연구에서는 유방암 조기 진단을 위하여 광 음향 단층촬영 방식의 영상 장치를 개발하고 팬텀을 이용하여 그 유용성을 평가하고자 한다. 튜브 팬텀과 닭 가슴살 팬텀을 제작하고 이동 평균 필터와 3~6 MHz의 대역폭을 갖는 대역 통과 여파기를 설계하여 잡음을 제거하고 시간 지연 빔 형성(delay-and-sum beamforming) 알고리즘을 이용하여 광음향 영상을 재구성 하였다. 연구 결과 영상의 재구성에 있어서 빔 형성 알고리즘을 적용하기 전에 대역 통과 여파기와 같은 신호 처리가 효과적임을 보였다. Recently, the photoacoustic imaging system has been widely and intensively developed, and has been shown the possibility of diagnosis for early stage cancer. In this study, we developed a photoacoustic tomography imaging system with a commercial ultra sound device and a linear array probe. A tube phantom and a chicken breast phantom was made for the possibility of a system as a breast cancer detection. A moving average filter and a band pass filter with 3~6 MHz bandwidth were developed for background noise elimination before delay-and-sum beamforming algorithm was used for image reconstruction. As a result, we showed that some signal processing procedure before beamforming was effective for the photoacoustic image reconstruction.
nrDNA-ITS 분자마커를 이용한 오미자(五味子) 종 감별 및 기원분석 -ITS 염기서열을 이용한 오미자(五味子) 감별-
김호경 ( Ho Kyoung Kim ),지윤의 ( Yun Ui Ji ),서형석 ( Hyeong Seok Seo ),이아영 ( A Young Lee ),천진미 ( Jin Mi Chun ),문병철 ( Byeong Cheol Moon ) 대한본초학회 2010 大韓本草學會誌 Vol.25 No.4
Objectives: The original plant species of Schisandrae Fructus (O-mi-ja) is prescribed as Schisandra chinensis BAILL., in Korea, but S. chinensis BAILL. and S. sphenanthera REHD. et WILS. in China. Moreover, fruit of several other species in genus Schisandra also have been used as the same herbal medicines. To develop a reliable method for correct identification of Schisandrae Fructus and to evaluate the phylogenetic relationship of S. chinensis and its related species, we analyzed internal transcribed spacer (ITS) sequences of nuclear ribosomal DNA (nrDNA). Methods: Twenty-four plant samples of three Schisandra species and one Kadsura species, S. chinensis BAILL., S. spenanthera REHD. et WILS., S. nigra Max. and Kadsura japonica DUNAL were collected from each different native habitate and farm in Korea and China. The nrDNA-ITS region of each samples were amplified using ITS1 and ITS4 primer and nucleotide sequences were determined after sub-cloning into the pGEM-Teasy vector. Authentic marker nucleotides were estimated by the analysis of ClastalW based on the entire nrDNA-ITS sequence. Results: In comparative analysis of the nrDNA-ITS sequences, we found specific nucleotide sequences including indels (insertions and deletions) and substitutions to distinguish C. chinensis, S. spenanthera, S. nigra, and K. japonica. These sequence differences at corresponding positions are avaliable nucleotide markers to determine the botanical origin of O-mi-ja. Moreover, we evaluated the phylogenetic relationship of four plant species by the analysis of nrDNA-ITS sequences. Conclusions: These marker nucleotides would be useful to identify the official herbal medicines by the providing of definitive information that can identify each plant species and distinguish it from unauthentic adulterants for O-mi-ja.