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형질전환된 식물세포에서 Sucrose 농도가 hGM-CSF 생산에 및 미치는 영향
이진옥,심두희,주치언,김동일,이동근,이재화,Lee, Jin-Ok,Shim, Doo-Hee,Joo, Chi-Un,Kim, Dong-Il,Lee, Dong-Geun,Lee, Jae-Hwa 한국식물생명공학회 2004 식물생명공학회지 Vol.31 No.2
본 연구에서는 hGM-CSF 유전자가 도입된 형질전환 담배세포에서 sucrose의 농도가 배양 배지내 분비된 hGM-CSF, 총분비 단백질, 그리고 단백질 분해효소에 미치는 효과를 보았다. 10, 30, 60, 90, 120, 150 g/L의 sucrose 농도로 배양한 결과 sucrose농도가 30g/L일 때 건조중량은 11.22g/L, 분비된 hGM-CSF의 양은 181.53 $\mu\textrm{g}$/L, 그리고 총단백질은 66.8 mg/L.로 시료채취 5일 때 가장 높은 값을 나타내었고, 단백질 분해효소는 sucrose농도가 120 g/L일때 2660 U/L.의 값을 나타내었다. 그러나 배양 10일째 sucrose농도 60 g/L에서 건조중량이 28.36g/L로 가장 높은 값을 나타낸 반면 분비된 hGM-CSF의 최대 값은 95 $\mu\textrm{g}$/L.로 sucrose농도가 150 g/L일 때 가장 높은 값을 나타내었다. The effects of sucrose concentration on the secretion of hGM-CSF, total protein and protease into the medium were investigated in transgenic tobacco cells. The dry cell weight (11.22 g/L), hGM-CSF (181.53 $\mu\textrm{g}$/L) and total protein (66.8 mg/L) were detected as highest at 30 g/L sucrose and protease activity (2660 U/L) was highest at 120 g/L sucrose after 5-day culture. However after 10-day culture, the maximum dry cell weight (28.36 g/L) was found at 60 g/L sucrose while the maximum hGM-CSF (95 $\mu\textrm{g}$/L) was appeared at 150 g/L sucrose. The total protein and protease activity was secreted as 52.28mg/L and 3430 U/L, respectively in the same culture.
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김진욱(Jin Wook Kim),주치언(Chi Un Joo),박진용(Jin Yong Park),이송애(Ae Song Lee),김인혜(In Hae Kim),이재화(Jae Hwa Lee) 한국환경성돌연변이발암원학회 2005 한국환경성돌연변이·발암원학회지 Vol.25 No.4
Antibiotic resistance of bacteria in the biofilm mode of growth contributes to the chronicity of infection and disease. The penetration of antibiotic, through biofilm developed in an in vitro model system was investigated. Antibiotic resistant bacteria (E. coli) were obtained from Culture Collection of Antibiotic Resistant Microbes. Ca-alginate bead used as simulated biofilm and a cell entrapment test using compressed air were experiment for the improvement cell viability. Antibiotic susceptibilities though biofilms was measured by assaying the concentration of antibiotic that diffused through the biofilm to minimal inhibition concentration (MIC). Survival of immobilized cells were reduced as compared to free cells. In case of antibiotic susceptible E. coli reduced continuously, but antibiotic resistant E. coli kept up survival rate constantly. Survival was showed after exposed to the antibiotics that the more treated antibiotic resistant E. coli and low concentration of antibiotics, the more survived.
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박진용,주치언,김진욱,이상현,이재화 신라대학교 자연과학연구소 2006 自然科學論文集 Vol.15 No.-
Bacteriocins are antibacterial proteins produced by bacteria that kill or inhibit the growth of other bacteria. Many lactic acid bacteria produce a high diversity of different bacteriocins. Since food safety has become an increasingly important interantional concern, the application of antimicrobial peptides from lactic acid bacteria that target food pathogens without toxic or other adverse effects has received great attention. This article gives an overview of bacteriocin applications, and characteristics.
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김진욱,정종근,주치언,이재화 신라대학교 자연과학연구소 2005 自然科學論文集 Vol.14 No.-
Probiotics는 "장내균종을 개선시켜 줌으로써 숙주동물에게 유익한 영향을 주는 것", "사람 또는 동물 장내 미생물의 균형을 개선시켜 숙주에 효과를 주는 살아있는 미생물 단독 또는 혼합배양물"이라는 의미의 용어이다. Probiotics의 조건에는 효능성, 안전성, 안정성, 분리성의 4가지 조건을 만족시켜야 하며, 식품의 상품성 향상, 면역증강, 유해효소의 합성 억제, 유당 불내증 개선, 장내 유해균의 증식 억제, 장내 균총의 정상화 등 다양한 효능을 가지고 있다. 이런 다양한 효능을 가진 probiotics는 건강식품 및 의료용의 소재, 화장용 소재, 사료 첨가제, 생 백신 개발 재료, 천연 항균물질 생산 등 다양한 곳에 응용되어 사용이 가능하다. Probiotics are living non-pathogenic micro-organisms, which as food ingredients also beneficially affect host's health. These non-pathogenic micro-organisms may be lactic acid bacteria, such as lactobacilli, lactococci, and bifidobacteria, or yeasts, such as Saccharomyces cerevisiae subspp. boulardii, or bacillus, such as B. subtilis, B. natto, B. licheniformis. Probiotics were originally used to influence both animal and human health through intestinal microbiota alterations. At present, the specific live microbial food ingredients and their effects on human health are studied both within food matrices and as single or mixed culture preparations. Probiotics such as lactobacilli have been reported to effect positively, gastrointestinal disorders such as diarrhoea (rotavirus-associated and antibiotic -associated), inflammatory bowel disease, pouchitis, irritable bowel syndrome, colorectal cancer, lactose absorption, Helicobacter pylori infection and constipation and are increasingly used as a treatment modality. Basic and clinical research should thus be associated to meet the enthusiasm that patients show for a therapeutical approach that they feel to be safer than most drugs currently proposed to them.
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