군대 내에서 웹기반의 당뇨병 조절 시스템 개발

주지현 ( Ju Ji-hyeon ),조재형 ( Cho Jae-hyong ),윤건호 ( Yoon Gun-ho ) 국군의무사령부 2005 대한군진의학학술지 Vol.36 No.1

스마트 도시의 개인정보 활용 및 데이터 거래시장 형성 가능성: 보건, 의료 데이터를 중심으로

주지현 ( Ju Jihyun ),김갑성 ( Kim Kabsung ) 한국부동산분석학회 2023 不動産學硏究 Vol.29 No.1

글릭콜릭산 필링 후 rhEGF and Palmitoyl pentapeotide-4의 피부 재생 효과

주지현 ( Ji Hyun Ju ),김도현 ( Do Hyeon Kim ),박건 ( Kun Park ),김남산 ( Nam San Kim ),김정희 ( Jeonghee Kim ) 한국미용학회 2020 한국미용학회지 Vol.26 No.1

Glycolic acid peel is a popular superficial peeling technique associated with low morbidity and prompt recovery. These processes, as well as the final step of corneodesmolysis, which mediates exfoliation, are often disturbed by environmental conditions, resulting in dry, flaky skin conditions. Therefore, aftercare is required to avoid these side effects and achieve the maximum possible improvement in skin condition. To evaluate the skin regeneration effect of rhEGF and palmitoyl pentapeptide-4 after glycolic acid peeling. A base gel, a gel containing rhEGF, and a gel containing palmitoyl pentapeptide-4 were prepared, and their safety was evaluated by patch test. The 30 healthy female volunteers (age 25-39 years) underwent a glycolic acid (20%) face peel once per week and then the prepared gels were applied to the face for 4 weeks. Stratum corneum hydration, transepidermal water loss (TEWL), and erythema index were then evaluated. No specific skin reaction in prepared gels was detected by a patch test. In the application group of rhEGF and palmitoyl pentapeptide-4, stratum corneum hydration is increased, TEWL and erythema index are reduced. Therefore, the skin regeneration effect was confirmed. The skin regeneration effect of the palmitoyl pentapeptide-4 application group was shown to be the best result in particular. The gels containing rhEGF and palmitoyl pentapeptide-4 were safe and effective for skin recovery after glycolic acid peeling. The palmitoyl pentapeptide-4-containing gel was superior to the rhEGF-containing gel in terms of skin regeneration after peeling.

PD-L1 발현 B세포를 통한 활성 T세포의 IL-17 분비 조절

우윤주 ( Yun Ju Woo ),조미라 ( Mi La Cho ),민준기 ( Jun Ki Min ),주지현 ( Ji Hyeon Ju ),주세영 ( Se Young Ju ),박성환 ( Sung Hwan Park ),김호연 ( Ho Youn Kim ) 대한류마티스학회 2008 대한류마티스학회지 Vol.15 No.2

목적: 최근 들어 면역조절능을 가지는 B세포의 존재에 관한 연구가 보고되고 있다. B세포의 특성 중 면역조절기능을 세포상호작용을 통해 연구하고자 본 실험에서는 B세포를 B세포 수용체(anti IgM)와 CD40에 대한 단클론항체(agonist anti mouse CD40 monoclonal antibody)로 자극하여 관찰하였다. 방법: 마우스의 비장에서 분리한 B세포를 lipopolysaccharide (LPS), B세포 수용체 또는 CD40에 대한 단클론항체로 자극하여 24시간 동안 배양하여 B세포에서 발현하는 공동신호분자(CD40, CD80, CD86, MHC-II, PD-1, PD-L1)를 유세포 분석기로 조사하였다. 그리고 활성화된 B세포를 anti CD3로 자극한 T세포와 공조 배양하여 T세포에서 발현하는 사이토카인을 중합효소연쇄반응(PCR, polymerase chain reaction)과 효소결합 면역흡수 분석법(ELSIA, enzyme-linked immunosorbent assay)으로 조사하였다. 결과: B세포에서의 공동신호분자는 자극(LPS, B세포 수용체 또는 CD40에 대한 단클론항체)에 대해 반응하여 모두 증가하는 경향을 나타냈으며 특히 B세포 수용체와 CD40에 대한 단클론항체로 자극하였을 경우 음성신호분자로 알려져 있는 PD-L1의 발현이 가장 의미 있게 증가되었다. B세포를 T세포와 공조배양 하면, T세포에서 분비되는 IL-17이 의미 있게 감소(nil: 1932±386 pg/mL vs anti IgM+agonist CD40mAb: 1193±334 pg/mL, p<0.01)하였다. 그러나 IFN-γ (nil: 4658±752 pg/mL, anti IgM+agonist CD40mAb: 5525±315 pg/mL)와 TNF-α (nil: 1445±178 pg/mL, anti IgM+agonist CD40mAb: 1,469±281 pg/mL)의 형성에는 변화가 없었다. 또한 공조배양을 통한 IL-17 생성 억제는 PD-L1 중성화 항체 처리로 회복되는 경향을 보였다. 결론: 이와 같은 연구로 B세포는 B세포 수용체와 CD40에 대한 단클론항체로 자극하면 PD-L1의 발현이 증가되고, T세포와 공조배양 시 IL-17분비를 뚜렷이 감소시킨다는 결과를 얻을 수 있었다. 이는 면역조절능을 가진 B세포는 PD-L1발현을 통해 결과적으로 IL-17의 생성을 억제시킨다는 것을 시사한다.

청각장애학교 교사의 교육과정에 대한 실천적지식의 내,적 정향 요인 분석

최성규 ( Sung Kyu Choi ),주지현 ( Ji Hyun Ju ) 한국특수교육문제연구소 2013 특수교육저널 : 이론과 실천 Vol.14 No.1

이 연구는 청각장애학교 교사의 교육과정에 대한 실천적 지식의 정향을 분석하기 위하여 전국의 청각장애학교에 재직하는 교사 310명의 설문지를 수집하였다. 5점 척도의 24문항으로 구성된 설문지는 상황적 정향 6문항, 사회적 정향 4문항, 개인적 정향 3문항, 경험적 정향 4문항, 그리고 이론적 정향 7문항으로 구성되어 있다. 교육과정 실천적 지식은 내적 정향 (개인적 정향과 경험적 정향)과 외적 정향(상황적 정향, 사회적 정향, 이론적 정향)으로 구분하였다. 설문지의 타당도와 신뢰도를 확보하기 위하여 요인 분석 및 신뢰도 분석을 실행하였고 일원배치분산분석, 상관관계, 중다회귀분석 등을 적용하였다. 청각장애학교 교사의 교육과정 실천적 정향은 경험적 정향과 함께 이론적 정향이 통계적으로 가장 높은 영향력을 제공하며, 다음으로 사회적 정향과 개인적 정향의 순으로 나타났다. 그러나 상황적 정향은 청각장애학교 교사의 교육과정 실천적 지식 구동에 영향력이 낮음을 알 수 있다. 이 연구에서는 또한 청각장애학교 교육과정의 실천적 지식의 정향에 대한 시사점을 제시하고 있다. The purpose of this study was to analyze the relation of the internal and external orientation factor of practical knowledge to curriculum of teachers who were working for students with hearing impairment. The questionnaire which is consisted with 24 questions was divided into five parts such as situational orientation, personal orientation, social orientation, experiential orientation, and theoretical orientation. Internal orientation factors of practical knowledge to curriculum were personal orientation and experiential orientation, however external orientation factors of practical knowledge to curriculum were situational orientation, social orientation, and theoretical orientation. 310 teachers who were working for students with hearing impairment were participated for this study. The validation, reliability, oneway ANOVA, correlation, and regression were utilized for this study. This study concluded that the orientation of practical knowledge to curriculum of teachers for students with hearing impairment was related with theoretical orientation, social orientation, and personal orientation based on experiential orientation. However, the situational orientation factor was not related with the curriculum practical knowledge.

역분화줄기세포의 류마티스내과 영역에서의 응용

정승민 ( Seung Min Jung ),주지현 ( Ji Hyeon Ju ) 대한류마티스학회 2013 대한류마티스학회지 Vol.20 No.5

Since induced pluripotent stem cell (iPSC) was first introduced by Yamanaka in 2006, it took only six years to win a Nobel Prize for his pioneering work. It is unusual to win a Nobel Prize for such recent research with a short history. Many scientists and clinicians are interested in iPSC for its potential application. Significant progression in this field has been made, while there remain many hurdles to overcome for application of iPSC technique in real clinics. In this review, the concept of reprogramming and the basic techniques of iPSC generation will be discussed for the reader`s convenience, followed by discussion of recent progress, followed by the topics of “disease modeling” and “cell therapy” with iPSC in the second half of this article. Several examples of rheumatologic application of iPSC will be provided in the main text. If rheumatologists could understand the merits and potentials of iPSC, opportunities for innovative research and therapy can be expanded.

류마티스관절염 활막세포에서 NF-${\kappa}B$ 신호전달을 통한 MIF의 SDF-1 생성 유도

조미라,박미경,김경운,오혜좌,이선영,박진실,허유정,주지현,민준기,이상헌,박성환,김호연,Cho, Mi-La,Park, Mi-Kyung,Kim, Kyoung-Woon,Oh, Hye-Jwa,Lee, Seon-Yeong,Park, Jin-Sil,Heo, Yu-Jung,Ju, Ji-Hyeon,Min, Jun-Ki,Lee, Sang-Heon,Park, Sung-Hwa 대한면역학회 2007 Immune Network Vol.7 No.1

Background: Stromal cell-derived factor (SDF)-1 is a potent chemoattractant for activated T cells into the inflamed Rheumatoid arthritis (RA) synovium. To determine the effect of macrophage migration inhibitory factor (MIF) on the production of SDF-1 in the inflamed RA synovium. Methods: The expression of SDF-1 and MIF in RA and Osteoarthritis (OA) synovium was examined by immunohistochemical staining. The SDF-1 was quantified by RT-PCR and ELISA after RA fibroblast like synoviocyte (FLS) were treated with MIF in the presence and absence of inhibitors of intracellular signal molecules. The synovial fluid (SF) and serum levels of MIF and SDF-1 in RA, OA and healthy control were measured by ELISA. Results: Expression of SDF-1 and MIF in synovium was higher in RA patients than in OA patients. The production of SDF-1 was enhanced in RA FLS by MIF stimulation. Such effect of MIF was blocked by the inhibitors of NF-${\kappa}B$. Concentrations of SDF-1 in the serum and SF were higher in RA patients than in OA patients and healthy control. SDF-1 and MIF was overexpressed in RA FLS, and MIF could up-regulate the production of SDF-1 in RA FLS via NF-${\kappa}B$-mediated pathways. Conclusion: These results suggest that an inhibition of interaction between MIF from T cells and SDF-1 of FLS may provide a new therapeutic approach in the treatment of RA.

줄기세포를 이용한 골관절염 치료

남유준 ( Yoojun Nam ),임예리 ( Yeri Alice Rim ),주지현 ( Ji Hyeon Ju ) 대한내과학회 2019 대한내과학회지 Vol.94 No.2

Osteoarthritis is a musculoskeletal disease representative of an aging society. As medical conditions are usually complicated in an aging population, osteoarthritis becomes more frequently encountered in the physician’s office. There is a growing need, therefore, for physicians to pay attention to this common orthopedic condition. Cartilage degeneration, arthritic pain, and joint dysfunction are major manifestations of osteoarthritis, and degenerated cartilage is difficult to repair with conventional treatment modalities. Scientists and physicians have developed various therapeutic strategies, including the use of stem cells. Here, we discuss previous and current progress in cartilage regenerative therapy against osteoarthritis. (Korean J Med 2019;94:145-151)

활동성 전신홍반루푸스 환자의 FSChigh Memory B 세포의 특징 분석

전주연 ( Joo Yeon Jhun ),김영주 ( Young Joo Kim ),주지현 ( Ji Hyun Ju ),박성환 ( Sung Hwan Park ),장숙희 ( Soog Hee Chang ),김호연 ( Ho Youn Kim ) 대한류마티스학회 2007 대한류마티스학회지 Vol.14 No.3

Objective: To determine phenotypic and functional characteristics of memory B cells in patients with systemic lupus erythematosus (SLE). Methods: The percentage of memory B cell subsets in peripheral blood mononuclear cells (PBMC) from normal control (n=11), inactive (n=15) and active (n=10) SLE patients was determined by Fluorescence Activated Cell Sorter (FACS). In addition, the activation status of memory B cells was measured by the surface expression of CD86 (B7-2). The production of antibodies to chromatin and dsDNA (IgG and IgM type) by isolated memory B cell subsets was examined by enzyme-linked immunosorbent assay (ELISA). Results: In this study, we analyzed 2 subtypes of memory B cells: FSC (Forward Side Scatter)low and FSChigh memory B cell. The percentage of both subtypes from active and inactive SLE patients was significantly reduced compared to that of normal controls (p<0.01). In addition, the expression of activation markers, CD86 on FSChigh memory B cells from active SLE patients was higher than those of inactive SLE patients and normal controls (p=0.014). Upon stimulation with CpG and IL-15 in vitro for 8 days, isolated FSChigh memory B cells from active SLE patients revealed augmented production of autoantibodies to chromatin and dsDNA. Conclusion: Our results suggest that abnormally activated FSChigh memory B cells from active SLE patients might be involved in spontaneous production of autoantibodies and induce transition from inactive to active phase of the patients.

폐암환자에서 급성호흡부전과 장천공을 동반한 분선충 감염증

김현식 ( Hyeon Sik Kim ),김유은 ( Yu Eun Kim ),윤은영 ( Eun Young Yun ),주지현 ( Ji Hyun Ju ),마정은 ( Jeong Eun Ma ),이기동 ( Gi Dong Lee ),조유지 ( Yu Ji Cho ),김호철 ( Ho Cheol Kim ),이종덕 ( Jong Deok Lee ),황영실 ( Young Sil 대한결핵 및 호흡기학회 2010 Tuberculosis and Respiratory Diseases Vol.68 No.1

Strongyloides stercoralis is an intestinal nematode that is a parasite to humans. The infecting filariform larvae of S. stercoralis enters the host body via the bloodstream, passes through the lungs, penetrates the alveoli, and then ascends the airway to transit down the esophagus into the small bowel. The infection can persist for decades without causing major symptoms and can elicit eosinophilia of varying magnitudes. Of note, this infection can also develop into a disseminated, often fatal, disease (hyperinfection) in patients receiving immunosuppressive corticosteroids. A 65-year-old man who was receiving corticosteroid therapy for the treatment of spinal stenosis was admitted to the emergency room with complaints of abdominal pain and severe dyspnea. We detected many S. stercoralis larvae in the sputum and in the bronchoalveolar-lavage sample collected by bronchoscopy. Here, we report a fatal case of strongyloidiasis with acute respiratory failure and intestinal perforation. In addition, we provide a brief review of the relevant medical literature.