오계란(烏鷄卵)이 MIA 골관절염 병태 모델에 미치는 영향

주인환,김동희,Joo, In-Hwan,Kim, Dong-Hee 대한본초학회 2017 大韓本草學會誌 Vol.32 No.6

Objectives : The purpose of this study is to investigate the preventive and therapeutic efficacy of osteoarthritis using a Yeonsan-Ogye egg. so, we researched at effects of Yeonsan-Ogye egg extract on MIA-induced Osteoarthritis animal models. Methods : Yeonsan-Ogye egg extract was administered 500 mg/kg/day, 1000 mg/kg/day and 2000 mg/kg/day to SD-Rat for 2 weeks. After that, osteoarthritis was induced with $60mg/m{\ell}$ of monosodium iodoacetate (MIA) and futher administration was continued for 4 weeks. 3D imaging of cartilage patella were obtained using a Micro-CT system and the pathology change of knee was observed by H&E and safranin-O staining. The weight bearing ratio was measured by incapacitance test meter. MMP-2, MMP-9, COMP, CTX II, calcitonin and glycosaminoglycan level in serum were measured using a ELISA. Results : Micro-CT and Histopathological analysis showed the volume of the patella cartilage and the proteoglycan contents were increased in all groups. also weight bearing ratio was decreased in all groups compared with control group. Calcitonin production was increased in and 2000 mg/kg/day group and glycosaminoglycan production was increased in all groups. In addition, MMP-2, MMP-9, COMP and CTX II production were decreased in 1000 and 2000 mg/kg/day groups respectively in comparison with control. Conclusions : The results for Yeonsan-Ogye egg showed prevention and treatment efficacy against arthritis at serum and the cartilage. These results may be used a remedy for new korea medicine to ease the symptoms mentioned above. also, suggest that Yeonsan-Ogye egg can be used preventive and therapeutic material for osteoarthritis.

토사자 에탄올 추출물이 UVB로 유도된 CCD-986Sk cell에서 주름개선 생리지표에 미치는 영향

주인환(In Hwan Joo),최학주(Hak Joo Choi),심부용(Boo Yong Sim),민가율(Ga Yul Min),김동희(Dong Hee Kim) 한의병리학회 2018 동의생리병리학회지 Vol.32 No.5

호남고속철도 차량 시스템 사양에 관한 연구

주인환(Joo In-Hwan),박광복(Park Kwang-Bok) 한국철도학회 2002 한국철도학회 학술발표대회논문집 Vol.- No.-

The Honam conventional railroad was already exceseed the limit capacity of transportation at some section. Therefore Honam railroad axis needs new high speed railroad line for increasing of transportation capacity now. This report was studied about the system technical specification of rolling stock based on new technology of high speed train of advenced countries, KHST and KTX for Honam high speed railroad.

함양산양삼과 열매 복합물의 항산화 및 항염증 효능에 대한 연구

이종상,주인환,박종민,김동희,Jong-Sang Lee,In-Hwan Joo,Jong-Min Park,Dong-Hee Kim 대한동의생리학회·한의병리학회 2023 동의생리병리학회지 Vol.37 No.3

The purpose of this study is to examine the antioxidant effect of hamyang wild-simulated ginseng and ginseng berry complex(HGC) and to investigate the mechanism of anti-inflammatory effect on macrophage cell line (RAW264.7 cells). For antioxidant activity of HGC was measured by total polyphenol contents were estimated with folin-ciocalteu method, and total flavonoid contents were estimated with aluminum chloride methods. And, assessed with 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis-3-ethylbenzothiazoline-6-sulphonic acid (ABTS) radical scavenging activity assay. MTT assay was performed to determine the effect of HGC on cell viability in RAW264.7 cells. In addition, anti-inflammatory effect of HGC was investigated in RAW264.7 cells. RAW 264.7 cells, in which an inflammatory response was induced by lipopolysarccharide (LPS), were treated with HGC, and nitric oxide production was measured by griess assay. And protein expression level of inflammatory cytokine and phosphorylation of MAPK signaling pathways were confirmed by western blot analysis. Total polyphenols and flavonoids contents in HGC were quantified, and increased DPPH and ABTS radical scavenging activity in a concentration-dependent manner was confirmed. In addition, HGC was significantly inhibited nitric oxide (NO) production and inflammatory cytokine (iNOS, IL-1β, IL-6, and TNF-α) expression in LPS-stimulated RAW264.7 cells. Also, HGC was significantly inhibited the phosphorylation of MAPK signaling pathways. These results suggest that HGC can be used as effective herbal medicine extract for antioxidant and anti-inflammatory effect. As a result, it is thought that HCG can be used as a functional material effective for antioxidant and anti-inflammatory effects. And it will be possible to promote industrialization using hamyang wild-simulated ginseng and ginseng berry, and to increase high-value-added.

꾸지뽕나무 잎과 우슬 복합물이 MIA로 골관절염이 유도된 Rat에 미치는 영향

정길호,주인환,김동희,Kil-Ho Cheong,In-Hwan Joo,Dong-Hee Kim 대한동의생리학회·한의병리학회 2023 동의생리병리학회지 Vol.37 No.3

This study was to investigate the effects of cudrania tricuspidata leaf and achyranthes japonica nakai complex (CAC) treatment on monosodium iodoacetate (MIA)-induced osteoarthritis rats. Osteoarthritis was induced by injection of MIA (3 mg) into right knee joints of rats. The rats were divided into 5 groups; Non-induced normal rat (Con, n=10), MIA-induced osteoarthritis rat (MIA, n=10), osteoarthritis rat treated with indomethacin 2 mg/kg (Indo, n=10), osteoarthritis rat treated with CAC 200 mg/kg (Low, n=10) or 400 mg/kg (High, n=10). The rats were treated orally for 14 days. On the last day of oral administration, the hind paw weight bearing of the experimental animals was measured using an incapacitance test meter. The interleukin-1β (IL-1β), interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), prostaglandin E2 (PGE2), cartilage oligomeric matrix protein (COMP) and, C-telopeptides of type II collagen (CTX-II) levels in serum were measured by ELISA. And Then, that gene expression (IL-1β, IL-6, TNF-α, COX-2) levels in cartilage were measured by qRT-PCR. Also, cartilage volume was measured by micro CT arthrography. Administration of CAC reduced the inflammatory cytokine (IL-1β, IL-6, TNF-α, PGE2) levels in serum and gene expression levels in cartilage, and the COMP and CTX-II level in serum, which is an indicator of cartilage degradation. In addition, the hind paw weight bearing and cartilage volume, which were decreased due to osteoarthritis, were significantly increased. In the future, if the results of clinical studies including studies on safety are supplemented, it is considered that it can be used as an oriental medicine treatment to improve or treat symptoms of osteoarthritis.

청혈플러스가 혈관내피세포에서 염증 지표인자에 미치는 영향

서동효,주인환,김동희,Seo, Dong-hyo,Joo, In-Hwan,Kim, Dong-Hee 대전대학교 한의학연구소 2018 한의학연구소 논문집 Vol.27 No.2

Objectives : Coronary and cerebrovascular disease with high mortality is a major factor in arteriosclerosis. Pro-inflammatory cytokines damage vascular endothelial cells, leading to vascular inflammation. These vascular inflammation can build up cholesterol and thrombus to cause atherosclerosis. Methods : In this study, we researched the effect of ChungHyul-Plus for vascular inflammation in human umbilical vein endothelial cells (HUVECs) stimulated with tumor necrosis $factor-{\alpha}$ ($TNF-{\alpha}$). Change in mRNA expression of inflammatory cytokines (CCL5, CXCL8, CX3CL1, and MCP-1), cell adhesion molecules (VCAM-1 and ICAM-1), and anti-inflammation modulators (KLF2 and eNOS) were quantified by qRT-PCR. Results : ChungHyul-Plus decreased expression of inflammatory cytokines and cell adhesion molecules and increased anti-inflammation modulators expression in $TNF-{\alpha}$ stimulated HUVECs. Conclusions : These results suggest that ChungHyul-Plus can be used in the treatment and prevention of vascular inflammation and arteriosclerosis.

천연물 복합제가 제 2형 당뇨 마우스에서 혈당 및 지질대사에 미치는 영향

서동효,주인환,김동희,Seo, Dong-hyo,Joo, In-Hwan,Kim, Dong-Hee 대전대학교 한의학연구소 2020 혜화의학회지 Vol.29 No.2

Objectives: This study aims to investigate the effects of diabetes mellitus care mixture (DCM) on blood glucose and lipid metabolism in type 2 diabetic mellitus mice. DCM consisted of lagerstroemia speciose, allium hookeri, momordica charantia, amaranthus tricolor, and boesenbergia rotunda, which have been proven to have antidiabetic properties. Methods: In this study, we researched the effects of DCM in type 2 diabetic mellitus mice. C57BLKS/J mouse groups had no treatment, db/db mouse randomly assigned to 2 groups, and treated with distilled water and DCM (200 mg/kg/day). Blood glucose levels and body weight were checked every week. After 4 weeks of treatment, liver function indicators (AST, ALT, and LDH) and lipid metabolites (triglyceride, total cholesterol, LDL-cholesterol, HDL-cholesterol) were measured with a biochemistry analyzer. Diabetic factors (insulin, resistin, and leptin) were measured with ELISA. Results: DCM was decreased blood glucose, diabetic factors, liver function indicators, triglyceride, total cholesterol, and LDL-cholesterol significantly. Also, HDL-cholesterol was significantly increased in DCM group. The bodyweight of DCM group decreased but, no significant difference with the control group. DCM may have the potential to improved diabetes mellitus by regulating blood glucose levels and diabetic factors. Also protecting from diabetic complications by adjusting liver function indicators and lipid metabolites. Conclusions: These results suggest that DCM to be used as an oriental medicine for diabetes, the results of clinical trials are needed.

방사선 형질전환 차조기와 백출 복합추출물이 퇴행성관절염 관련 매개체에 미치는 영향

심부용(Boo-Yong Sim),주인환(In-Hwan Joo)김성규(Sung-Kyu Kim),지중구(Joong-Gu Ji) 한국응용과학기술학회 (구.한국유화학회) 2021 한국응용과학기술학회지 Vol.38 No.2

본 연구는 방사선 형질전환 차조기와 백출복합물(차조기 복합물)이 퇴행성관절염 관련 매개 체에 미치는 영향을 확인하고자 MIA(monosodium iodoacetate)로 퇴행성관절염을 유도한 랫드로 평가 하였다. 차조기 복합추출물을 2주 동안 25, 50, 100 ㎎/㎏/day의 용량으로 경구 투여하고 랫드의 우측 관절 내 공간에 MIA를 주입한 후 동일 용량을 4주 동안 지속 투여하였다. 이후, 혈청 바이오마커와 무릎 관절 분석의 형태학 및 조직병리학적 분석에 기초한 치료 효과를 평가하였다. 대조군 랫드와 비교하 였을 때 차조기 복합추출물은 혈청 내 염증 및 골 대사 마커(TNF-α, MMP-3, COX-2, PGE 2 , COMP, Aggrecan)의 생성량을 유의하게 감소시켰다. 이와는 반대로 연골 흡수 매개체인 CTX-2 생성을 증가시켰으며, 방사선 형질전환 차조기는 무릎 연골과 활막을 효과적으로 보존하였다. 그 결과, 차조기 복합물은 퇴행성관절염 증상을 개선하였다. 따라서, 차조기 복합물은 퇴행성관절염 관리를 위한 식품 소재로 사용될 수 있다. The present study aimed to evaluate the effects of radiation mutant Perilla frutescens var. crispa and Atractylodes macrocephala Koidzumi complex extract(Perilla frutescens var. crispa complex extract) on the mediators related to degenerative arthritis in a monosodium iodoacetate-induced rat model of degenerative arthritis. Perilla frutescens var. crispa complex extract was administered orally at doses of 25, 50 or 100 ㎎/㎏/day for 2 weeks before direct injection of monosodium iodoacetate (3 ㎎/50 μl of 0.9% saline) into the intra-articular space of the rats’ right knees. The rats subsequently received the same doses of oral Perilla frutescens var. crispa complex extract for another 4 weeks. It was evaluated that the treatment effects based on serum bio-markers, and morphological and histopathological analysis of the knee joints. Compared with those in negative control rats, the Perilla frutescens var. crispa complex extract treatments significantly reduced the serum levels of inflammation, bone metabolism markers (i.e., TNF-α, MMP-3, COX-2, PGE 2 , COMP, and Aggrecan). Otherwise, it was significantly increased the production of CTX-2 in cartilage absorption mediators. In addition, the Perilla frutescens var. crispa complex extract treatments effectively preserved the knee cartilage and synovial membrane. As a result, it indicates that the Perilla frutescens var. crispa complex extract improved degenerative arthritis symptoms. Thus, the Perilla frutescens var. crispa complex can be used in food material for the management of degenerative arthritis.

Indomethacin으로 유발된 생쥐의 위점막 손상에 대한 쑥을 함유한 천연물 복합제의 효과

정성연 ( Seong-yeon ),주인환 ( Jeong In-hwan Joo ),박종민 ( Jong-min Park ) 대전대학교 한의학연구소 2022 혜화의학회지 Vol.32 No.1

Objectives : This study was aimed to investigate whether gastritis care mixture (GCM) with artemisi a can prevent indomethacin-induced gastric damages. GCM consisted of artemisiae argyi folium, diosc oreae rhizoma, crataegi fructus, zizyphi fructus, and glycyrrhizae radix. Methods : Antioxidant activities were evaluated using 2,2-dipheny-1-picrylhydrazyl (DPPH), 2,2'-a zinobis-(3-ethylbenzothiazoline-6-sulfonic acid (ABTS), and ferric reducing antioxidant power (FRA P) method. GCM were pretreated 6 h before indomethacin treatment on rat gastricmucosal epithelial BGM1 cells. Cytoprotection effects eavluated by MTT. [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide]. Reverse-transcription PCR (RT-PCR) and Western blot was performed to check anti-inflammatory actions. Different doses of GCM were administrated intragastrically before the indomethacin administration in mouse models. After killing, addition to gross and pathological evaluation of ulcer, the expressions of inflammatory mediators were analyzed by enzyme-linked immunosorbent assay (ELISA). Results : GCM was very effective in preventing indomethacin-induced gastric damages in a low dose. indomethacin increased the expression of cyclooxygenase-2 (COX-2) and decreased the expression of 15-hydroxyprostaglandin dehydrogenase (15-PGDH) and Heme oxygenase-1 (HO-1), but GCM significantly attenuated indomethacin-induced COX-2 expression. Interestingly, GCM induced expression of 15-PGDH and HO-1. These in vitro findings were exactly validated in indomethacin-induced gastritis mouse models. GCM groups showed significantly ameliorated patheologic lesion compared to the control group. The serum levels of proinflammatory cytokines including interleukin-6 and tumor necrosis factor were significantly attenuated in the GCM group. However, these preventive effects of G CM were dependent on dosage of GCM; higher dose above 200mg/kg paradoxically aggravated indomethacin-induced inflammation. Conclusions : GCM is a candidate substance which can attenuate NSAIDs-induced gastritis on potent anti-inflammatory action.

고 함량 Chondroitin이 MIA 골관절염 병태 모델의 혈액 내 관련 병리 지표와 체중 부하에 미치는 영향

민가율(Ga-Yul Min),박종민(Jong-Min Park),주인환(In-Hwan Joo),심부용(Boo-Yong Sim),최학주(Hak-Joo Choi),김화영(Hwa-Young Kim),김진(Jin Kim),이명선(Myung-Sun Lee),김동희(Dong-Hee Kim) 한국식품영양과학회 2019 한국식품영양과학회지 Vol.48 No.3

본 연구는 골관절염에 대한 예방 및 개선 가능한 신규 건강기능식품 소재 개발을 위해 chondroitin sulfate sodium을 90% 이상 함유한 chondroitin을 MIA로 관절염을 유발하기 2주 전부터 유발 후 4주 동안 경구 투여하였다. 실험종료 후 염증 및 골 대사 지표에 대한 혈액학적 지표와 체중 부하, micro-CT 분석을 진행한 결과, COX-2, PGE₂, LTB₄ 등의 염증 매개체와 CTX-Ⅱ, COMP 등의 골 파괴 매개체 생성을 유의적으로 감소시켰으며, ACAN, GAGs 등의 골 생성 매개체 생성이 유의적으로 증가하였다. 이와 같은 chondroitin의 골관절염 병리 지표에 대한 혈액학적 개선은 체중 부하측정 결과를 통해 뒷받침하고 있으며, micro-CT 검사에서 확인된 연골 부피의 보존 결과가 이를 더욱 증명하고 있다. 이전 연구 결과 철갑상어에서 추출한 chondroitin에서는 상위 염증 매개체인 cytokine(IL-1β, TNF-α)의 발현을 감소시키고 골관절염 촉진 인자인 PGE₂, NO의 합성을 억제해주는 것을 확인하였다. 소에서 추출한 chondroitin에서도 염증 촉진 인자인 PGE₂, COX-2, LTB₄ 를 억제해줄 뿐만 아니라 골 생성(CTX-Ⅱ, COMP) 및 연골 생성(ACAN, GAGs) 대표 인자를 확인하였다. 따라서 기존 관절 낭액에서 진행한 실험과 혈액학적 실험에서 모두 항염증에 대한 유의성 있는 결과를 도출함으로써 본 연구에서 사용된 고 함량 chondroitin은 전임상 결과를 바탕으로 추후 임상시험이 보완된다면 골관절염에 대한 효과적인 건강기능식품 소재로 활용할 수 있을 것으로 기대된다. Osteoarthritis is a disease that affects the articular cartilage and osseous tissue, and can be worsened by aging, overweight status, and post-traumatic arthritis. The present study examined the effects of chondroitin on the bone metabolism and the inflammatory response in a monosodium iodoacetate (MIA)-induced rat model of osteoarthritis. Chondroitin was administered orally at doses of 300 mg/kg, 600 mg/kg, or 900 mg/kg for 2 weeks before a direct injection of monosodium iodoacetate (3 mg/50 μL of 0.9% saline) into the intra-articular space of the rats’ right knees. The rats subsequently received the same doses of oral chondroitin for another 4 weeks. The treatment effects were evaluated based on serum bio-markers and weight-bearing distribution, and morphological analysis of the knee joints. Compared to the control rats, the chondroitin treatments reduced significantly the serum concentrations of inflammation and bone destruction markers (i.e., COX-2, PGE₂, LTB₄, CTX-Ⅱ, and COMP), and increased significantly the concentrations of bone regeneration markers, ACAN and GAGs. In addition, the chondroitin treatments effectively preserved the knee cartilage and the destruction in tip of a femur. Moreover, the chondroitin treatments increased the weight-bearing distribution by 7∼24%. The results suggest that chondroitin prevented and alleviated the osteoarthritis symptoms. Hence, chondroitin may be a novel heath food material for the management of osteoarthritis. Therefore, high concentrations of chondroitin may be a novel health food material to manage clinical osteoarthritis in human patient.