전통의약분야의 지적 재산권 보호를 위한 대상 발굴 및 법적 · 제도적 보장 방안 연구

김홍준(Kim Hongjun),이상정(Lee Sangjung),주영승(Ju Youngsung) 한국한의학연구원 2002 한국한의학연구원논문집 Vol.8 No.2

2종 天南星의 外部 및 內部形態에 대한 연구

김홍준(Kim Hongjun),윤주봉(Yun Jubong),주영승(Ju Youngsung) 한국한의학연구원 2006 한국한의학연구원논문집 Vol.12 No.2

In the original plant of Arisaematis Rhizomas, the features of external and internal shape of Arisaema amurense var. serratum NAKAI and A. amurense MAXIM. are as follows. 1. In the external shape of original plant, Arisaema amurense var. serratum NAKAI has high stem, an oval-elliptic leaflet and a serrate leaf margin. But A. amurense MAXIM has a relatively low stem, an upside oval leaflet and no serrate leaf margin. 2. On the herbs character of original plant, Arisaema amurense var. serratum NAKAI is small in height and diameter, but A. amurense MAXIM is relatively large in. height and diameter. 3. On the gathered herbs character of original plant, the section of Arisaema amurense var. serratum NAKAI is brightly white, easily spilt and strong scent. But the section of A. amurense MAXIM is thin yellowish brown, not easily spilt and weak scent. Also the grade of gathered herbs, both can be classified by diameter and external scent. 4. In the current herbs character, Korean and Sichuan Province products are irregullar shape and have no scent, Jilin Province products are regular shape. Also, Guangxi Province products are small size and concave in one side, Hebei Province products are irregular size and shape. 5. In internal shape of original plant, epidermal cell of Arisaema amurense var. serratum is very tight. With the except of micro difference in parenchyma cell of cortex, on the whole there are nearly no differences with A. amurense MAXIM. Also, samely in the internal shape, according to collecting sites, epidermis exhibits a lot layer in curving state and secreting duct is developed and vascular bundle and exists between parenchyma cell of cortex. In the future, additional study is needed to distinguish herb and effect between same genus-degree of relatedness.

廣藿香과 土藿香의 外部 및 內部形態硏究

김홍준(Kim Hongjun),최정(Choi Jeong),주영승(Ju Youngsung) 한국한의학연구원 2006 한국한의학연구원논문집 Vol.12 No.2

Pogostemonis and Agastachis Herba are the whole of Pogostemon cablin (BLANCO) BENTH or Agastache rugosa (FISCHER et MEYER) O. KUNTZE (family Labiatae) which is produced in all part of Korea and China. This drug is used for removing dampness by means of aromatics in oriental medicine. The standard formula of this drug is important from the viewpoint of the quality control. A characteristic discrimination of internal and external morphological standard in original plants and herbal states of Pogostemonis and Agastachis Herba are as follows. 1. The external characteristics: Pogostemon cablin has hairs and brown-like in stem, elliptical fruit. In the other hand, Agastache rugosa has no hairs and red-like in stem, obovatic trigone fruit. 2 The physical characteristics: Pogostemon cablin is gray in whole, has hairs in stem and numerous hairs of ash in leaf. In the other hand, Agastache rugosa is yellow-green in whole, has no hairs in stem Specially the latter has deep-green colour and numerous hairs presenting mostly at lower epidermis in leaf. 3. The physical characteristics in currents: Pogostemon cablin is brown, has hairs and round-like stem In the other hand, Agastache rugosa is green or yellow-green, has no hairs and tetragon in stem. 4. The internal characteristics: Pogostemon cablin has progressed spongy tissue ill epidermal cell of leaf and many rank of epidermal cell ill stem In other hand, Agastache rugosa has 1 rank palisade tissue in leaf and few rank of epidermal cell in stem. In the external shape, it was possible that herbs were distinguished according to artificial classification and that same genus-degree of relatedness among herbs could be distinguished by more precise and active observation. In the shape of real herbs, I compared current herbs in market with original herbs which were just collected or were on the course of drying. In addition, it was possible that the internal shape could be identified by using microscope after butanol series. Though it was impossible to make distinction of herbs which are not current in my search contents, this search contents will be a standard for applying herbs in the future. An Additional standard establishment including physiochemical reaction and gene research is required in order to supplement the fault of this search.

石菖蒲의 內外部形態 硏究

최고야(Choi Goya),김홍준(Kim Hongjun),주영승(Ju Youngsung) 한국한의학연구원 2006 한국한의학연구원논문집 Vol.12 No.3

The purpose of this study was to introduce the differential standard of the types of Acori Graminei Rhizoma. We established the classificatory standard according to the external and internal morphology for the several types of Acori Graminei Rhizoma. 1. External shape: Acorus gramineus has smaller size and fibroidal section. A. calamus has bigger size and cancellate section. A. tatarinowii has middle size and fibroidal section. Anemone altaica has very small long-spindle shape and flat section that has 6-12 row of vascular bundle. 2. Internal shape: Acorus gramineus has some bundle sheath fiber, but A. calamus has not. A. tatarinowii has many calcium oxalate crystalloid around bundle sheath. Anemone altaica has obscure cambium. All sample except A. altaica, in the mass, has scattered bundle, thin cell wall, broad cortex and well-developed endodermis. For the future, such results will be used as the basic source of additional research, and a far-reaching comparative study is needed to distinguish between many kinds of same genus-degree of relatedness.

형태적 특징과 유전자분석을 통한 半夏 감별 연구

김홍준(Kim Hongjun),이미영(Lee Miyoung),홍성미(Hong Seongmi),고병섭(Ko Byoungseob),주영승(Ju Youngsung) 한국한의학연구원 2002 한국한의학연구원논문집 Vol.8 No.1

4종류 木香類의 外部 및 內部形態와 이화학패턴연구

김홍준(Kim Hongjun),강경식(Kang Kyoungsik),최고야(Choi Goya),김호경(Kim Hokyung),정승일(Jeong Seungil),주영승(Ju Youngsung) 한국한의학연구원 2006 한국한의학연구원논문집 Vol.12 No.3

The purpose of this study was to introduce the differential standard of Mok-Hyaeng Radix species. We established the classificatory standard according to the external and internal morphology and the pattern in 4 kinds of Mok-Hyaeng Radix. The results follow: 1. On the external morphology, Radix of Vladimiria souliei had a sticky material in the head of roots and it was yellowish white in the cut surface. It attached to teeth during chewing. Radix of Aucklandia lappa (=Saussurea lappa) was dark grayish brown and was grayish brown in the cut surface. It had special odor and was not attache to teeth chewing it. On the other hand, Radix of Inula helenium was dark brown. In the cut surface, cortical layer was different from wood layer. Radix of Aristolochia contorta was yellowish brown. In the cut surface, it was not smooth and cortical layer was different from wood layer. 2. On the internal morphology, Radix of Vladimiria souliei had many fascicular fibers and scattered large oil chambers in the bast ray, xylem ray, and parenchyma. On Radix of Aucklandia lappa (=Saussurea lappa), it was difficult to find out the trace when the cork layer was exfoliated. The cambium formed circle and the large oil chambers were scattered in the phloem and xylem On the other hand, external cortical layer of Radix of Inula helenium was detached and cambium was formed to indistinct circle and large oil chambers were scattered in the phloem and xylem. On Radix of Arustolochia contorta, the large oil chambers were scattered in the surface layer, bast part was relatively broad and cambium formed circle. 3. On the TLC pattern, Radix of Aristolochia contorta, Inula helenium; Aucklandia lappa, and Vladimiria souliei were chromogenic to violet and purple according to sulfuric acid colour pattern, especially faint on Aristolochia contorta. 4. On the pattern analysis of HPLC, we compared the content and the pattern of constunolide and dehydrocostus lactone, Radix of Aucklandia lappa was similar to Radix of Vladimiria souliei and Radix of Aristolochia contorta was similar to Radix of Inula helenium. On the total HPLC component pattern, constunolide and dehydrocostus lactone were similar in all four types, but it showed the Rf 5-6 peak was only in Radix of Aristolochia contorta, not in Radix of Inula helenium, AuckLandia lappa, and Vladimiria souliei. It is considered the results of this study will be furnished the basis to succeeding studies and it is needed to extensive comparative study for the same genus-degree of relatedness.

한의학 고문헌 분석을 통한 무궁화의 약용부위와 기대효능 연구

최고야(Choi Goya),윤태숙(Yoon Taesook),추병길(Choo Byungkil),이아영(Lee Ayeong),채성욱(Chae Sunguk),주영승(Ju Youngsung),김호경(Kim Hokyoung) 한국한의학연구원 2007 한국한의학연구원논문집 Vol.13 No.2

Hibiscus syriacus is rarely used herb. For finding up new applications of this herb, the medicinal parts and expected efficacys that recorded in 24 classics of oriental medicine were inquired. As a result of the literature review, medicinal parts and expected efficacys of Hibiscus syriacus were as follows: The medicinal parts were mainly flower and bark But the root, branch, leaf and fruit were mentioned too. Some unidentified medicinal parts seemed to be branch or bark. The mentioned symptoms that prescribed Hibiscus syriacus are intestinal 'wind', red-white dysentery, thirst with fever after dysentery, insomnia, 'wind' syndrom, leukorrhea, regurgitation, etc. And this herb was mentioned that applied externally for scabies, hemorrhoids, prolapse of the rectum, headache, extracted tooth, etc. Therefore, bleeding intestinal diseases, insomnia, vaginal or uterine hemorrhage(for internal use), scabies or mycosis, hemorrhoids, anal fistula, prolapse of the rectum, headache(for external use) were presented as expected efficacys of Hibiscus syriacus. In future, studies for verifying the efficacys are needed.

獨活의 外部 및 內部形態와 이화학패턴연구

김홍준(Kim Hongjun),김자영(Kim Ja-Young),최고야(Choi Goya),정승일(Jeong Seungil),주영승(Ju Youngsung) 한국한의학연구원 2006 한국한의학연구원논문집 Vol.12 No.3

The purpose of this study was to introduce the differential standard of the types of Angelicae Pubescentis Radix. We established the classificatory standard according to the external and internal morphology and the physicochemistrical pattern for the four types of Aucklandiae Radix. The results follow: 1. At the external shape, Angelica pubescens f. biserrata has rising lenticel and dark brown surface, Its section is light gray and its xylem is yellow gray. Aralia continentalis has big stem mark in upper portion, its section has no dense tissue with yellow gray. Heracleum hemsleyanum has dark brown surface and little lenticel, its cortical layer is white yellow and xylem is isabella and powdered. 2. At the internal shape, Angelica pubescens f. biserrata has phloem with half of the root and rare vessel. Aralia continentalis has phloem with two-thirds of the root and it has broad cutting and putting vessel that formed 1-2 row. Heracleum hemsleyanum also has phloem with half of the root and wide scattered latex tube and many large vessel. 3. At the TLC pattern, Heracleum hemsleyanum has remarkable dark spot at R 0.23 on the sulphuric acid color pattern test, but others have faint. 4. At the HPLC pattern, all samples have generally similar patterns. But Angelica pubescens f. biserrata shows diminutive continentalic acid content and the peak at Rt 20.278min comes out on Heracleum hemsleyanum, but do not come out on Aralia continental is and Angelica pubescens f. biserrata. Heracleum hemsleyanum has remarkable peak at Rt 20.278min, but shows no peak at Rt 29.023min unlike Aralia continentalis or Angelica pubescens f. biserrata. Also Aralia continentalis and Angelica pubescens f. biserrata show one remarkable peak at Rt 29.023, but Heracleum hemsleyanum do not show. Consequently, Aralia continentalis and Angelica pubescens f. biserrata are comparable whit continentalic acid content and Heracleum hemsleyanum is comparable with the peak at Rt 20.273 and Rt 29.023. So it is thought that content of continentalic acid and the peaks at Rt 20.278 and Rt 29.023 can apply to differentiate a species from others. It is considered the results of this study will be furnished the basis to succeeding studies and it is needed to extensive comparative study for the same genus-degree of relatedness.

천왕보심단(天王補心丹)∘쌀발효추출물(GABAː gamma-aminobutyric acid 30%) 복합물의 抗스트레스에 대한 유효성 연구ː무작위 배정 및 이중맹검

홍초영 ( Choyoung Hong ),서상권 ( Seo Sang Gwon ),나승곤 ( Seunggon Na ),조호성 ( Hosong Cho ),이미경 ( Mi-kyung Lee ),진무현 ( Mu Hyun Jin ),노석선 ( Seok-seon Roh ),주영승 ( Youngsung Ju ) 대한본초학회 2023 大韓本草學會誌 Vol.38 No.2

Objectives : From this study, we sight to identify Anti-stress effect of Chenwangbosim-Dan (Tianwangbuxin-Dan) Extract∘Fermented Rice Extract (GABA ː gamma-aminobutyric acid 30%) Complex Methods : In order to measure the degree of functional evaluation of tension relief due to stress, it was evaluated whether the psychological indicators VAS(Visual Analog Scale) and POMS(Profile Of Mood State) were improved. Which is generally used for stress measurement and has a high frequency of clinical use. All clinical trial subjects were required to take the drug once a day at 9 ± 30 min in the morning for 2 weeks. Results : After intake, the group that Chenwangbosim-Dan(Tianwangbuxin-Dan) Extract∘Fermented Rice Extract (GABA ː gamma-aminobutyric acid 30%) Complex had a statistically significant improvement effect compared to before intake due to an improved 30.81% of the VAS scores. The POMS-T score improved 54.13%, the POMS-V score improved 117.5% and the POMS-F score improved 59%, which had a statistically significant effect compared to Placebo group (p<0.001). There is a significant difference between two group, so even considering the placebo effect, the anti stress effect was confirmed in the Chenwangbosim-Dan (Tianwangbuxin-Dan) Extract∘Fermented Rice Extract (GABA ː gamma-aminobutyric acid 30%) Complex intake group. Conclusions : The above results showed that the Chenwangbosim-Dan(Tianwangbuxin-Dan) Extract∘Fermented Rice Extract (GABA ː gamma-aminobutyric acid 30%) Complex had an anti-stress effect. Therefore, it is expected to be developed as a safe and comfortable mental care health functional food that can reduce the risk of drug abuse.

木防己와 粉防己의 藥用 및 非藥用部位에 따른 鑑別

이금산(Guemsan Lee),김영식(Youngsik Kim),박경범(Kyoungbum Park),백지성(Jiseong Baek),김형우(Hyungwoo Kim),김홍준(Hongjun Kim),조수인(Suin Cho),이제현(Jehyun Lee),주영승(Youngsung Ju) 한국한의학연구원 2011 한국한의학연구원논문집 Vol.17 No.1

Object : As though the roots of Stephania tetrandra and Cocculus trilobus are used for medicinal purposes, climbing stems of these plants which do not have medicianl value are often adulterated and distributed in markets. Therefore, distinguishing the medicinal parts of these plants from the non-medicinal parts is necessary. Method : Samples of Stephania tetrandra and Cocculus trilobus were collected and authenticated. They were separated into root(StR, CtR) and climbing stem(StS, CtS), and then examined using stereoscope and microscope. Result : 1. Morphological characteristics: StR contained abundant powder and had a lotus flower-patterned section whereas StS had less powder and a wheel-patterned section. CtR had an irregular longitudinally wrinkled surface and a superb pink flower-patterned section whereas CtS had a regular furrowed surface and wheel-patterned section. 2. Anatomical characteristics: StR was abundant in starch grains and crystals (calcium oxalate), whereas StS lacked these features. In contrast to CtS, the width of the CTR medullary rays was equal or greater than that of the wood rays. Additionally, CtS contained high number of crystals but CtR did not. Conclusion : Morphological and anatomical characteristics are useful for identifying non-medicinal climbing stems of S. tetrandra and C. trilobus.