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가스치환포장과 감마선조사 병용 처리한 최소가공 무의 미생물학적 안전성
김재경,조철훈,김현주,이주운,황한준,변명우,Kim, Jae-Kyung,Jo, Cheo-Run,Kim, Hyun-Ju,Lee, Ju-Woon,Hwang, Han-Joon,Byun, Myung-Woo 한국식품과학회 2005 한국식품과학회지 Vol.37 No.1
최소가공 무의 미생물학적 안전성을 확보하고 품질을 보존하기 위하여 가스치환포장과 감마선 조사를 병용 처리하여 4에서 2주간 저장하면서 일반 호기성 미생물, 대장균군, 젖산균 및 pH의 변화를 각각 조사하였다. 일반 호기성 미생물의 변화를 관찰한 결과 저장기간과 포장 방법에 관계없이 감마선 조사에 의해 유의적으로 줄어들었으며 처리 당일의 경우 2kGy의 방사선 조사에 의해 $CO_{2}$나 $CO_{2}/N_{2}$ 포장구가 함기 포장구 보다 유의하게 미생물을 감소하였다. 대장균군 역시 저장기간과 포장 방법에 관계없이 감마선 조사에 의해 유의적으로 줄어들었으며 젖산균도 저장기간과 포장 방법에 관계없이 감마선 조사에 의해 유의적으로 줄어들었다. pH의 변화에 있어서는 감마선 조사와 포장방법에 의한 유의한 차이를 보이지는 않았지만 가스치환포장을 하였을 때 pH가 낮아지는 경향을 보였으며 저장기간 동안 pH 증가하였다. 이러한 결과로 볼 때 최소가공 무의 미생물학적 안전성을 확보하고 품질을 보존하기 위해서는 2kGy의 감마선 조사와 가스치환포장의 병용처리가 효과적이라 사료된다. White radish was minimally processed, packed with air, $CO_{2}$ (100%), and $CO_{2}/N_{2}$ (25/75%), and irradiated at 0, 1, and 2 kGy, and its microbiological quality and pH were investigated during storage for 2 weeks at $4^{\circ}C$. Irradiation significantly reduced total aerobic, coli-form, and lactic acid bacteria counts. Modified atmosphere packaging (MAP) enhanced microorganism control during storage. Acidity decreased by MAP but was restored during storage, Irradiation did not affect sample pH. Results show irradiation at 2 kGy combined with MAP can enhance microbiological safety and quality of minimally processed radish.
반응표면 분석법을 이용한 계란 난황 레시틴 분리 최적조건
장애라,임동균,전희준,조철훈,김일준,이무하,Jang, Ae-Ra,Lim, Dong-Gyun,Jeon, Hee-Joon,Jo, Cheo-Run,Kim, Il-Joon,Lee, Moo-Ha 한국축산식품학회 2007 한국축산식품학회지 Vol.27 No.4
난황으로부터 레시틴 고함유 인지질을 회수하기 위하여 전처리, 표면반응분석기법을 실시한 결과 난황 인지질 분리조건(난황액 100g 기준)은 알코올 농도 95.83%, 알코올 첨가량 1: 6.51, 균질온도 $40.2^{\circ}C$, 20,000 rpm에서 5분간 균질한 후 원심 분리하여 여과 후 진공농축하고 아세톤 150 mL로 1회 세척하여 분리한 것으로 수율은 인지질 64.8%이상 레시틴 함량 55.04%의 인지질 분리를 위한 공정을 확립하였다. The optimum conditions for the extraction of lecithin from egg yolk were determined using response surface methodology (RSM). On the basis of the results of preliminary experiments, the most effective values were selected. The effects of three independent variables, dilution ratio, solvent composition, and extraction temperature on the response of crude egg lecithin (g) were then determined. The optimum conditions for egg lecithin separation obtained using ridge analysis were 6.51, 95.83%, and $40.2^{\circ}C$ for the dilution ratio, solvent composition, and extraction temperature, respectively. Using the optimum conditions, 55.04% of crude lecithin in total phospholipid can be obtained from 100 g liquid egg yolk. The experimental values (56.21% crude lecithin in total phospholipid) agreed with the predicted values.
닭 다리 분쇄육에 초고압 처리시 Phosvitin의 첨가가 미생물학적 품질과 지방 및 단백질 산화에 미치는 효과
정사무엘,강민구,김일석,남기창,안동욱,조철훈,Jung, Samooel,Kang, Min-Gu,Kim, Il-Suk,Nam, Ki-Chang,Ahn, Dong-Uk,Jo, Cheo-Run 한국축산식품학회 2012 한국축산식품학회지 Vol.32 No.2
본 연구는 초고압 처리가 부분육으로 유통되는 닭 다리 분쇄육의 저장 안전성에 미치는 효과와 난황 phosvitin 첨가가 지방 및 단백질의 산화 안정성에 미치는 효과를 확인하기 위하여 수행되었다. 분쇄된 닭 다리육에 phosvitin을 각각 500 및 1000 mg/kg 첨가 후 비가열 또는 가열하고, 초고압 처리(300 및 600 MPa, 5분, $15{\pm}3^{\circ}C$)에 따른 총 호기성 미생물, 지방 및 단백질산화 및 육색의 변화를 $4^{\circ}C$에서 7일간 저장 하면서 측정하였다. 연구 결과 생육에서 300 및 600 MPa 처리에 의해 총 호기성 미생물의 증식이 저장 7일 후 각각 4 및 5 Log CFU/g 정도 억제 되었으며(p<0.05), 600 MPa 처리에 의해 지방 및 단백질산화가 촉진됨이 확인되었다(p<0.05). 초고압 처리압력 증가에 따른 $L^*$ 값의 증가와 $a^*$ 및 $b^*$ 값의 감소가 확인되었다(p<0.05). 가열육은 초고압 처리와 무관하게 총 호기성 미생물의 증식이 일어나지 않았으며, 저장 7일 후 600 MPa 처리에 의한 지방의 산화가 확인되었고, 단백질의 산화는 저장 3일차에서만 600 MPa처리에 의해 유의적으로 증가함이 나타났다(p<0.05). 이상의 결과를 종합해 볼 때 초고압 처리는 부분육으로 유통되는 닭 다리 분쇄육의 저장 안전성을 높일 수 있으며, 초고압 처리시 발생하는 지방 및 단백질의 산화와 육색의 변화를 억제하기 위한 항산화제로서 phosvitin의 이용은 제한적일 것으로 생각된다. The objective of this study was to investigate the effect of high pressure (HP) processing on shelf life, as well as the addition of phosvitin on lipid and protein oxidation stability of minced chicken leg meat. Minced chicken leg meat was mixed with yolk phosvitin at 500 or 1000 mg/kg meat levels, and divided into raw and cooked groups. Then, the samples were subjected to HP at 0.1, 300, and 600 MPa. The total aerobic bacteria, lipid and protein oxidation, along with instrumental meat color ($L^*$, $a^*$, and $b^*$value) of the samples were measured during storage for 7 d at $4^{\circ}C$. In raw meat, the number of total aerobic bacteria was decreased by HP at 300 MPa (4 Log reductions) and 600 MPa (5 Log reductions) after 7 d of storage (p<0.05). HP at 600 MPa increased lipid oxidation of samples at all storage days and protein oxidation of samples during storage at 3 and 7 d. HP induced the changes of meat color by increase of $L^*$ value and decrease of $a^*$ value (p<0.05). The total aerobic bacteria was not detected in the cooked samples, regardless of HP pressure, and the lipid or protein oxidation of the cooked sample treated by 600 MPa was higher than that of the control (0.1 MPa) on day 7 or control on day 3, respectively (p<0.05). The results suggested that HP can improve the shelf life of minced chicken leg meat. However, phosvitin might be a limited antioxidative agent for the improvement of oxidation stability induced by HP.
강민구,김현주,장애라,감동근,윤관식,조철훈,Kang, Min-Gu,Kim, Hyun-Joo,Jang, Ae-Ra,Gam, Dong-Keun,Yun, Gwan-Sik,Jo, Cheo-Run Institute of Agricultural Science 2012 Korean Journal of Agricultural Science Vol.39 No.1
This study was carried out to investigate the effects of dietary supplementation of quercetin ($Kocetin^{TM}$, QR) on antioxidative activity and meat quality of beef cattle (Holstein-Friesian). Beef cattle were divided into 3 groups; dietary supplementation of QR at 21 (n=4) and 42 ppm (n=3), and non-supplemented control (n=4). The QR comprised of 10% of quercetin. After slaughtering the beef cattle, loins were obtained and analyzed. Dietary supplementation of QR at 42 ppm showed significantly higher final pH of loin but did not affect the water holding capacity, drip loss, cooking loss, surface color, total phenolics content, 1,1-diphenyl-2-picrylhydrazyl radical scavenging activity. Dietary QR showed no difference in both 2-thiobarbituric acid reactive substances and volatile basic nitrogen values. Textural characteristic results also showed no difference, except for cohesiveness. Cohesiveness was significantly higher in loin from beef cattle treated by dietary QR at 42 ppm when compared to control. Results suggest that dietary QR, which has only 10% of quercetin is not sufficient to have positive biochemical effects on beef meat quality.
UV-C 및 전자선 조사가 막걸리의 품질에 미치는 영향
김현주 ( Hyun Joo Kim ),이경행 ( Kyung Haeng Lee ),용해인 ( Hae In Yong ),조철훈 ( Cheo Run Jo ) 한국식품저장유통학회(구 한국농산물저장유통학회) 2013 한국식품저장유통학회지 Vol.20 No.1
This study was conducted to evaluate the changes in the quality of rice wine treated with UV-C and electron bean irradiation during its storage at 4℃ for 15 days. The EB irradiation was found to be more effective than the UV-C irradiation for microbiological control. The pH tended to be increased by UV-V, EB irradiation, and storage period. Acidity was significantly higher in rice wine treated with EB irradigtion than those of control and UV-C irradiation during storage period, The L*-calue of the samples treated with UV-C and EB irradiation tended to be proportionately higher than that of the control, but this was not consistent during storage period. The a&-calud of the sample treated with EB was higher than that of the others, but this was not consistent during thestorage period. The reducing sugar content was higher in the control at day 0, but it rapidly decreased compared with the control during the storage. The results indicate that EB irradiation was more effective than UV-C irradiation in controlling microorganisms of rice wine. However, a further study is needed to minimize the rice wine quality deterioration caused by UV-V or EB irradiation during storage.
신지혜,정석근,한기성,장애라,채현석,유영모,안종남,이주운,조철훈,이완규,함준상,Shin, Ji-Hye,Jeong, Seok-Geun,Han, Gi-Sung,Jang, Ae-Ra,Chae, Hyun-Seok,Yoo, Young-Mo,Ahn, Chong-Nam,Lee, Ju-Woon,Jo, Cheo-Run,Lee, Wan-Kyu,Ham, Jun-Sang 한국축산식품학회 2008 한국축산식품학회지 Vol.28 No.3
The aim of this work was to examine the reduction in antigenicity of milk proteins in powdered milk by gamma irradiation which is increasingly used for food safety. Skim milk powder samples were exposed to irradiation doses of 1, 5, and 10 kGy. A greater reduction of ${\alpha}_{S1}$-casein and ${\beta}_{A1}$-casein was found than ${\alpha}_{S0}$-casein and ${\beta}_{A2}$-casein by capillary electrophoresis. Competitive indirect ELISA and passive cutaneous anaphylaxis tests using guinea pigs showed a reduction in antigenicity of powdered milk by 10kGy gamma irradiation. These results indicated that gamma irradiation reduce allergenicity of milk proteins by structural changes of ${\alpha}_{S1}$-casein and ${\beta}_{A1}$-casein, and can be useful for dairy products.