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압출방식으로 제작된 PCL/NaCl 와이어를 이용하여 제작된 이중기공 세포지지체 제작
조용상(Yong Sang Cho),송기명(Gi Myoung Song),안상현(Shang Hyun An),이세환(Se Hwan Lee),조영삼(Young-Sam Cho) 대한기계학회 2010 대한기계학회 춘추학술대회 Vol.2010 No.11
Firstly, we prepared PCL/NaCl mixture with 20wt% NaCl constituents. After that, we fabricated PCL/NaCl wire with 400 and 600 micrometer using extrusion technique. Firstly, one layer was composed with (PCL wire/metal wire) repeatedly. The second layer was composed by same process but 90 degree rotated. This layer stacking process was executed repetitively when the height is reached at the designed height. After that, PCL wires became a porous scaffold under the environmental temperature over 60 degrees Celcius. Subsequently, metal wires were removed from the resultant. We soak this scaffold into D. I. water to leach out salt. Finally, we could get bi-pore scaffolds.
SLUP+WNM 세포지지체에서 osteoblast cell과 saos-2 cell의 in-vitro 세포 배양 특성 비교
조용상(Yong Sang Cho),홍명화(Myung Wha Hong),김소연(So-Youn Kim),이승재(Seung-Jae Lee),이준희(Jun Hee Lee),김영율(Young Yul Kim),조영삼(Young-Sam Cho) 대한기계학회 2013 대한기계학회 춘추학술대회 Vol.2013 No.12
In this study, a novel technique for dual-pore scaffold fabrication using SLUP (salt leaching using powder) and WNM (wire network molding) was proposed. To fabricated dual-pore scaffold, firstly, PCL with size of 63~100mm and NaCl powders with size of 100~180mm were prepared. Secondly, prepared PCL and NaCl powders were mixed using the stirrer at a certain ratio. Subsequently, stainless-steel needles with size of 500mm were inserted into a designed stainless steel mold. And then, the mixture powders were filled in the stainless-steel mold. Afterword, after the mold was pressurized at 30 MPa for 1 min using a lab-made compressor, the mold was heated to melt PCL powders in an oven at 100℃ for 20 min. Thereafter, after the stainless-steel needles were removed in the stainless-steel mold, the PCL/NaCl structure was separated from the stainless-steel mold. And then, the PCL/NaCl structure was soaked in D.I water for 24 hours using a sonicator to leach out the NaCl particles and dry for 24 hours in the desiccator. Consequently, the remaining PCL structure became the dual-pore scaffold. To analyze surface and cross-section characteristics of scaffolds, scaffolds was observed using SEM. Additionally, cell-culture experiments were performed using CCK-8 assay, ALP and compared with the bio-plotter and SLUP scaffolds.
SLUP (Salt-Leaching Using Powder) 세포지지체의 생체 내외 거동 특성 평가
조용상(Yong Sang Cho),권미령(Quan Meiling),홍명화(Myoung Wha Hong),김영율(Young Yul Kim),조영삼(Young-Sam Cho) 대한기계학회 2015 대한기계학회 춘추학술대회 Vol.2015 No.11
In tissue engineering, salt-leaching method which is one of the conventional methods has been widely used, because if its easiness and simplicity. However, conventional salt-leaching method has some disadvantages: lack of interconnectivity and usage of solvent or high-pressure. Therefore, to overcome these drawbacks, our team developed SLUP (Salt-Leaching Using Powder) method which needs no solvent and high-pressure and provides good interconnectivity. In this study, proposed SLUP scaffold was compared to PED (Precision Extruding Deposition) scaffold using in-vivo and in-vitro experiments. Firstly, SLUP scaffold with ratio of 1:5 wt % (PCL/NaCl) was prepared. Secondly, PED scaffold was prepared with 500 micrometer strand size and 1000 micrometer distance of strands. After that, to assess cell-culture characteristics of osteoblast, scaffolds were assessed by in-vitro CCK-8 assay for 14 days. Furthermore, for in-vivo experiments, rats having calvaria defect were used.
Spray 방법을 이용한 다양한 고분자의 표면거칠기 생성
안상현(Sang Hyun Ahn),이세환(Se Hwan Lee),조아라(A Ra Jo),조용상(Yong Sang Cho),송기명(Ki Myoung Song),조영삼(Young-Sam Jo) 대한기계학회 2011 대한기계학회 춘추학술대회 Vol.2011 No.10
PS, Nylon6, PC, and PMMA which are dissolved by solvents such as THF, Formic acid, and acetone are used for manufacturing rough surface. We varied several parameters, such as pressure, spraying distance, and spraying time to study the effect of parameters to the surface topology and sprayed-particle adhesion. Firstly, via spin coating, we prepared PS, Nylon6, PC, PMMA thin films to be sprayed. If we sprayed the dissolved polymer onto slide glass directly, the sprayed-particle adhesion to the surface of glass is very weak. Therefore, we make thin films on the slide glass with the same polymer as the spraying dissolved polymer. After that, we sprayed the dissolved polymer onto the thin film of same polymer with varying parameters, i.e., pressure, spraying distance, and spraying time. Using the sprayed surface, we characterize the topology with SEM and surface energy with contact angle measurement.