보웬병의 임상 및 병리조직학적 연구

최희봉 ( Hee Bong Choi ),조옥자 ( Ok Ja Joh ),박시룡 ( See Ryoug Park ),김낙인 ( Nack In Kim ) 대한피부과학회 2006 대한피부과학회지 Vol.44 No.9

Background: Bowen`s disease, also known as squamous intraepidermal carcinoma, is a pre-malignant skin tumor with a potential to progress to an invasive carcinoma. Objective: The purpose of this study was to investigate the clinical and histopathological characteristics of Bowen`s disease. Methods: A total of 45 patients with Bowen`s disease were enrolled in this study. The hospital charts and histopathological slides from 1994 to 2005 were reviewed retrospectively at Seoul Veterans Hospital. Results: There were thirty-seven cases (82%) of single lesions and eight cases (18%) of multiple lesions. Thirty cases (81%) of solitary Bowen`s disease were located in non sun-exposed areas. These included the trunk, extremities and genital area in decreasing order. Seven cases (19%) of solitary Bowen`s disease occurred on sun-exposed areas including the face and the dorsum of the hand in decreasing order. Analysis of the clinical character of Bowen`s disease was performed and results were as follows: the most common type was patch (20 cases; 44%), plaque (14 cases; 31%) and pigmented lesions (12 cases: 27%). Analysis of the histopathological character of Bowen`s disease was performed and results were as follows: the most common type was irregular (18 cases; 46%), psoriasiform (11 cases; 28%) and verrucous, atrophic type in decreasing order. Of 18 cases where surgical removal was performed, there were 2 case (11%) of recurrence. Of 16 cases where cryotherapy treatment was administered, there were 3 cases of recurrence. And of 4 cases where CO2 laser therapy was used, there was 1 case (25%) of recurrence, in 2 cases where multiple or large-sized lesions were observed, clinical recovery processes were monitored after applying isotretinoin and 5-FU combined therapy, as well as surgical removal and cryotherapy combined therapy. Conclusion: In Bowen`s disease among Koreans, there were many distinctive clinical and histopathological features compared to those of non-Korean cases. (Korean J Dermatol 2006;44(9):1058~1064)

고엽제에 노출된 베트남전 참전군인들의 피부질환에 대한 임상적 고찰

노진혁 ( Jin Hyuk Rho ),조옥자 ( Ok Ja Joh ),박시룡 ( See Ryong Park ),허충림 ( Choong Rim Haw ),한지윤 ( Jee Yoon Han ) 대한피부과학회 2005 대한피부과학회지 Vol.43 No.4

피부의 발육과정에서 α-와 γ-catenin의 면역조직화학적 발현양상

이나영 ( Na Young Lee ),조옥자 ( Ok Ja Joh ),박시룡 ( See Ryong Park ),김미경 ( Mi Kyung Kim ),이선 ( Sun Lee ),송계용 ( Kye Yong Song ) 대한피부과학회 2004 大韓皮膚科學會誌 Vol.42 No.6

태아 피부의 발육과정에서 저분자량 케라틴(K8/18)의 발현

고유수 ( Yoo Soo Ko ),조옥자 ( Ok Ja Joh ),이재승 ( Jai Seung Lee ),송계용 ( Kye Yong Song ) 대한피부과학회 2007 대한피부과학회지 Vol.45 No.10

Background: The epidermis and adnexal epithelium might express different types of keratin (K) during fetal development. Objective: The objective is to observe the distribution of K8/18 in the skin of fetuses and to find out the distinction of expressions of K8/18 during fetal development. Methods: Immunohistochemical analysis was applied to the skin of the scalp and sole of 42 fetuses ranging from 10 to 39 weeks of gestation. Immunohistochemical staining with monoclonal antibodies with CAM5.2 using LSAB kit against K8/18 was conducted. Results: In the skin of the scalp, K8/18 was expressed in the periderm and basal layer of epidermis from the 10th week to the 31st week of fetal gestation. K8/18 was expressed in the hair germ, bulge and basal cells of fetal the infundibulum and sebaceous glands. Root sheath cells were weakly positive but matrix cells were negative. The expression of K8/18 was negative in the basal layer of the sole. Merkel cells, which are located in the basal layer and upper dermis, were positive from the 12th week of gestation. Terminal eccrine ducts and acinar cells were positive after the 20th week of gestation. Conclusion: K8/18 in the skin of the scalp and sole of fetuses were expressed in different ways. The expression of K8/18 in the basal cells of the sole were negative while basal cells of the epidermis of the scalp were positive transiently from the 12th to the 31st week of gestation. Early hair germ cells and bulge cells were expressed strongly in hair follicles. Terminal eccrine ducts and acini were expressed strongly in the eccrine gland. Merkel cells located in the basal layer and papillary dermis also express K8/18. (Korean J Dermatol 2007;45(10):1013∼1019)

두피에 발생한 사마귀양 이각화종

최희봉 ( Hee Bong Choi ),고유수 ( You Soo Ko ),조옥자 ( Ok Ja Joh ),박시룡 ( See Ryong Park ),송계용 ( Kye Yong Song ) 대한피부과학회 2005 대한피부과학회지 Vol.43 No.6

태아피부 발육과정 상피에서 클라우딘-1의 발현

김혜경 ( Hye Kyoung Kim ),고유수 ( Yoo Soo Ko ),조옥자 ( Ok Ja Joh ),이재승 ( Jai Seung Lee ),송계용 ( Kye Yong Song ) 대한피부과학회 2008 대한피부과학회지 Vol.46 No.8

Background: Claudins are integral membrane proteins at tight junctions of simple epithelium and have an important role in cell-cell adhesion and barrier function. Their altered expression modifies the biological function and behavior of epithelial cells. Objective: The purpose of this study was to observe the distribution and the degree of expression of claudin-1 during fetal skin development. Methods: The expression of claudin-1 in the fetal skin was analyzed after immunohistochemical stain was performed on paraffin embedded tissue of sole and scalp of 34 human fetuses, ranging from 10 to 39 weeks of gestational age, using anti-human monoclonal antibody against the claudin-1. Results: Claudin-1 appeared strongly in the sole skin at the 12th week of gestation thereafter expressed more in middle layer in epidermis of the sole and the scalp. Inner root sheaths and early hair germ cells were strongly positive in fetal hair development. Eccrine gland epithelium was positive in later stages. Focal positive expression in superficial layer and strong in middle layer were noted in oral mucosa side of the lip. Conclusion: Claudin-1 showed slightly different expression in skin and oral mucosa and has a important role in the development of tight junction and barrier formation in skin and mucosa during fetal stage. (Korean J Dermatol 2008;46(8):1048~1055)

피부 발육과정에서 데스모콜린 1의 발현에 관한 면역조직화학적 연구

최지현 ( Ji Hyun Choi ),김은정 ( Eun Jung Kim ),박향준 ( Hyang Joon Park ),조옥자 ( Ok Ja Joh ),송계용 ( Kye Yong Song ) 대한피부과학회 2012 대한피부과학회지 Vol.50 No.9

Background: Desmocollins (Dsc) are calcium-dependent transmembrane glycoproteins of desmosomes that are important in the junction complex of epidermis and maintain structural integrity of the skin from external stressors. Among three Dscs (Dsc 1, 2, 3), Dsc 1 and 3 are distributed on skin. Objective: The purpose of this study was to observe the Dsc 1 distribution pattern on the skin and oral mucosa during fetal development. Methods: Skin was obtained from the sole and scalp of 33 fetuses, ranging from 10 to 37 weeks of gestational age. Immunohistochemical staining was performed on the paraffin-embedded tissue using a Dsc 1 monoclonal antibody. Results: Dsc 1 was expressed in the suprabasal layer but not in the basal layer of the epidermis of the sole at the 10th week of gestation. Thereafter, Dsc 1 expression further increased in the suprabasal layer with initiation of stratification and increased gradually in the granular layers of the sole and scalp epidermis. Dsc 1 was strongly expressed in the superficial layer of the infundibulum and inner root sheath of the hair follicle but was not expressed in the sebaceous cells or other hair components. The eccrine duct epithelium was focally and weakly positive for Dsc 1 expression. Furthermore, Dsc 1 was not expressed in oral mucosa, although the oro-cutaneous portion was strongly expressed in the superficial layer. Conclusion: Dsc 1 was strongly expressed in the suprabasal cells of the epidermis during fetal skin development, and expression increased gradually in the granular layer and inner root sheath of the hair follicle. However, Dsc 1 was not expressed in basal cells or in oral mucosa. Dsc 1 may play a role in the maintenance of epithelial integrity as part of desmosomes.

원전 : 태아 피부의 발달과정에서 데스모글레인-3 표현에 대한 면역조직화학적 연구

김준범 ( June Bum Kim ),박향준 ( Hyang Joon Park ),이재승 ( Jae Seung Lee ),조옥자 ( Ok Ja Joh ),송계용 ( Kye Yong Song ) 대한피부과학회 2010 대한피부과학회지 Vol.48 No.7

Background: Desmogleins are calcium-dependent transmembrane glycoproteins of the desmosome that form an import component of the junction complexes of epithelial cells. Desmogleins are involved in maintaining the structural integrity of tissues. So far, four different desmogleins (Dsg1, Dsg2, Dsg3 and Dsg4) have been identified. Objective: The purpose of this study was to observe the distribution pattern of desmoglein-3 in the fetal skin during development. Methods: Skin was obtained from the sole, scalp and lip of 34 fetuses that ranged in age from 10 to 39 weeks of gestational age. Immunohistochemical staining was performed on the paraffin embedded tissue using anti-human monoclonal antibody against the desmoglein-3. Results: The expression of desmoglein-3 in the epidermis appeared in the basal layer of the sole at the 10th week of gestation age. Thereafter, a stronger expression was noted in the middle layer of the sole and scalp epidermis. The basal layer had a stronger expression than did the other layers of the epidermis, followed by the middle and superficial layers. A stronger expression of desmoglein-3 in hair was noted in the outer root sheath, the bulge cells and the eccrine duct cells. The expression of desmoglein-3 in the lip mucosa was strong in both the basal and middle layers, while the skin side of the mucosa showed a stronger expression in basal layer. Conclusion: These results suggested that desmoglein-3 plays an important role in the development and differentiation of the epidermis and skin adnexa in the fetal stage, and especially in basal and suprabasal layers. (Korean J Dermatol 2010;48(7):558∼566)

Self-Inflicted Dermatoses의 임상적 고찰

김학주 ( Hak Ju Kim ),고유수 ( Yoo Soo Ko ),이재승 ( Jae Seung Lee ),조옥자 ( Ok Ja Joh ) 대한피부과학회 2009 大韓皮膚科學會誌 Vol.47 No.3

Background: Self-inflicted dermatosis is a kind of self-inflicted skin disease. Most Vietnam veterans from Korea have visited the Veterans Hospitals for dermatological examination to determine the relationship between their skin diseases and Agent Orange. We were suspicious of the possibility that several Vietnam veterans intentionally produced their own skin lesions. Objective: We conducted this study to determine the clinical characteristics of self-inflicted dermatoses of Korean Vietnam veterans. Methods: From January, 1999 to December, 2007, a total 24 Vietnam veterans who were diagnosed as having self-inflicted dermatoses during their dermatological examinations at our hospital were included in this study. We investigated their clinical presentation, the laboratory examinations, the pathologic examinations, the medical records and the associated diseases. Results: Itching was the most common subjective complaint and the duration of the itching was as follows; 20 to 30 yrs (50.0%), 1 to 10 yrs (25.0%), 10 to 20 yrs (20.8%) etc. The skin lesions were all multiple, and the involved area was as follows; upper extremities (83.3%), trunk (75.0%), lower extremities (70.8%) etc. There were two categories of clinical skin presentation. The first was the cases with lesion that were thought to be made by physical trauma such as stinging or excoriation, and the second more common cases had lesions that were thought to be made by chemical agents (70.8%). These cases resembled allergic or toxic irritant dermatitis and the lesions were composed of vesicles, erosions, ulcers and crusts that were within an arm`s reach. Only 4 cases admitted to intentionally inflicting their skin lesions because of intense itching, and the others denied that they made the skin lesions by themselves. Skin biopsies were carried out in 19 of the total 24 cases, and the most common finding was spongiotic dermatitis (57.8%). Conclusion: This study showed the distribution and characteristics of self-inflicted dermatoses, and the study provides fundamental data for dermatologists to assess patients with self-inflicted dermatoses. (Korean J Dermatol 2009;47(3):259∼267)

피부의 발육과정에서 데스모글레인-1의 발현

김지혜 ( Ji Hye Kim ),김은정 ( Eun Jung Kim ),박향준 ( Hyang Joon Park ),조옥자 ( Ok Ja Joh ),송계용 ( Kye Yong Song ) 대한피부과학회 2011 大韓皮膚科學會誌 Vol.49 No.9

Background: Desmosomes are cell-cell adhesion complexes that provide mechanical integrity to keratinocytes by linking them to keratin intermediate filaments. Desmosomes are composed of two major transmembrane proteins, desmoglein and desmocollin. In humans, four desmoglein isoforms have been identified: Dsg1, Dsg2, Dsg3, and Dsg4. Desmogleins are Ca2+-dependent adhesion molecules and play important parts in the formation and maintenance of desmosomes. Desmoglein-1 is the main skin-associated desmosomal cadherin. It is expressed throughout the epidermis, but most prominently in the differentiated layers. Objective: The purpose of this study was to observe the distribution pattern of desmoglein-1 in the skin and oral mucosa during fetal development. Methods: Skin was obtained from the sole and scalp of 35 fetuses, ranging from 10 to 37 weeks of gestational age. Immunohistochemical staining was performed on paraffin embedded tissue using anti-human monoclonal antibody against desmoglein-1. Results: Expression of desmoglein-1 in the epidermis appeared in the upper layer of the sole, but the basal layer was negative at the 10th gestational age. Thereafter, stratification began with stronger expression in the middle layer than in the basal layer of the sole and scalp epidermis. Expression in the middle spinous layer is stronger in the fetal period than in other layers of the epidermis. Expression in the superficial layer seemed to increase in later stages. Expression of desmoglein-1 in hair was strong in the infundibulum, inner root sheath, sebaceous glandular epithelium, and eccrine duct epithelium. Expression of desmoglein-1 in oral lip mucosa was very weak or negative in the upper half of the mucosal epithelium, though the lower half was strongly positive, while the skin side of the mucosa was similar with the sole skin. Conclusion: Desmoglein-1 may play a complementary role in the maintenance of epithelial integrity along with other desmogleins, because desmoglein-1 distribution is slightly different from that of desmoglein-3 in epidermis, hair and mucosa in fetal skin development. (Korean J Dermatol 2011;49(9):786~794)