원유로 오염된 지역으로부터 분리한 생물계면활성제 생산균주, Pseudoalteromonas sp. HK-3의 특성조사

조수희,오계헌,Cho, Su-Hee,Oh, Kye-Heon 한국미생물학회 2010 미생물학회지 Vol.46 No.4

The purpose of this work was to investigate the characteristics of a biosurfactant-producing bacterium isolated from crude-oil contaminated soils. During the incubation of strain HK-3 with 1% crude-oil, bacterial growth pattern, the amount of biosurfactant production, and pH changes were monitored. In order to examine the effect of supplemented carbons on the production of biosurfactant, cultivation of HK-3 cells in BH media with different carbons (e.g. glucose, dextrose, mannitol, citrate, or acetate) revealed that the production of biosurfactant reached the maximal level at the 72 h incubation with mannitol, which the area of clear zone was measured to approximately 7.64 $cm^2$. Identification test using the BIOLOG system, morphology study based on scanning electron microscopy and the 16S rRNA sequence-based phylogenetic analysis assigned strain HK-3 to a Pseudoalteromonas species, designated as Pseudoalteromonas sp. HK-3 which was registered in GenBank as [FJ477041]. 본 연구의 목적은 원유에 오염된 지역의 토양시료로부터 분리한 생물계면활성제 생산균주의 특성을 조사하기 위하여 실시하였다. 1% 원유를 포함하는 배지에서 균주 HK-3의 배양기간 동안, 생장, 생물계면활성제의 생산, pH의 변화 등을 조사하였다. 생물계면활성제의 생산능력이 뛰어난 균주인 HK-3를 선별하여, 이 균주의 배양기간에 따른 생장 변화와 생물계면활성제의 생산량, 그리고 pH에 대하여 관찰하였다. HK-3는 배양 36시간이 경과하였을 때, 가장 많은 양의 생물계면활성제를 생산하였다. 생물계면활성제 생산에 대한 부가탄소원의 효과를 알아보기 위하여 HK-3를 다른 부가탄소원(예, glucose, dextrose, mannitol, citrate, acetate)과 함께 배지에서 배양하였다. 그 결과, 생물계면활성제의 최대생산량은 mannitol을 포함하는 BH 고체평판배지에서 관찰되었으며, 투명대의 면적은 직경은 약 7.64 cm2로 측정되었다. 분리균주의 생리학적 및 생화학적 특성을 조사하였으며, 주사전자현미경을 통하여 불규칙한 막대형의 세균으로 관찰되었다. BIOLOG 시스템과 16S rRNA 염기서열을 이용한 계통유전학적 분석을 통하여 동정하여 Pseudoalteromons 종으로 확인되어 Pseudoalteromons sp. HK-3로 명명하였으며, GenBank에 [FJ477041]로 등록하였다.

VAR모형을 이용한 주택정책 실증 연구

조수희(Cho Su Hee),정재호(Chung Jae Ho) 한국부동산학회 2007 不動産學報 Vol.31 No.-

  1. CONTENTS<BR>  (1) RESEARCH OBJECTIVES<BR>  This study researches and focuses on how the housing polices and the macroeconomic variables of each Korean government have an effects on the house sales prices in Korean real estate market. The objectives of this study are to evaluate the housing policies of each Korean governments with in economical environments, and derive the current issues from this study.<BR>  (2) RESEARCH METHOD<BR>  For evaluating, this study first makes the index of the housing policies and then analysis results by using VAR model.<BR>  (3) RESEARCH RESULTS<BR>  First, the analysis results of correlation shows that the index number of industrial production and the total amount of currency have not an big effect on the house sales prices, and the index number of the housing policies and the housing loan keep pace with the house sales prices. The analysis results of Granger casuality shows that there is a Granger casual relationship between the house sales prices and the housing loan, and also shows that there is a two-way Granger casual relationship between AA and the house sales prices. Second, in the sixth Korean regime which accomplishing economic growth with rising the house sales prices, the research is analyzed that the housing market was accompanied well by the government housing policy"s goal as showing a negative reaction to the AA"s shock and a positive reaction to the housing policy"s shock; on the other hand, Noh Moo-Hyun administration react negatively to the AA"s shock and positively to the housing policy"s shock for a while, but soon it reacts negatively to the housing policy"s shock. Third, before Noh Moo-Hyun administration, the analysis results of variancy decomposition shows that the housing loan and AA have an effect on the prediction of house sales prices; however, in Noh Moo-Hyun administration, the analysis results of variancy decomposition shows that the housing policy factors have an effect on the prediction of house sales prices primarily.<BR>  2. RESULTS<BR>  The government should not make a housing plan which has a strong demand restraint and an excessive supply in order to drop in housing prices right now. The government is requested to design a comprehensive housing policy plan in a long-term, not a short-term, taking into consideration such factors like durability. consistency and stability in the coming years and years. Also, the government should not stabilize real estate market by only using the housing policy, they should consider current sociological and economical situation without the government"s intervention if they could.

Stenotrophomonas maltophilia OK-5에 의한 TNT 함유폐수 (pink water)의 생물학적 처리와 Nitroreductase (pnrB ) 유전자의 RT-PCR 정량화

조수희(Su-Hee Cho),조윤석(Yun-Seok Cho),오계헌(Kye-Heon Oh) 한국생물공학회 2009 KSBB Journal Vol.24 No.6

본 연구는 TNT 분해능이 우수한 세균인 S. maltophilia OK-5를 이용하여 TNT 함유 폐수인 pink water의 미생물학적 처리 가능성에 대한 연구를 하였다. Pink water에 함유된 TNT 제거를 위해 S. maltophilia OK-5를 교반탱크 반응조에서 배양한 결과 pink water 내에 존재하는 100 mg/L의 TNT를 배양 6일 만에 완전 분해하였다. Hydride-Meisenheimer complex에서 유래하는 진한 적갈색은 배양기간 내에 증가하였으며, 이를 정량적으로 확인하였다. 본 연구에서 pink water에 잔류하는 TNT 뿐만 아니라 2,4-dinitrotoluene, 2,6-dinitrotoluene, 2,4-dinitro-6-hydroxytoluene 등의 대사산물도 HPLC 분석방법으로 측정하였으며, GC-MS를 사용하여 확인하였다. 또한 pink water에서 배양된 S. maltophilia OK-5에서 발현되는 nitroreductase (pnrB)의 유전자 발현정량을 real time PCR로 측정하였다. 그 결과 배양 5일째 pnrB copy 수가 10³ 이상 증가하는 것을 확인하였다. The biological treatment of TNT-containing wastewater, known commonly as pink water, was investigated using a stirred tank reactor with Stenotrophomonas maltophilia OK-5 bacterial culture. S. maltophilia OK-5 exhibited effective degradation of TNT contained in pink water, completely degrading TNT (100 mg/L) within 6 days of incubation. The dark-red brown color derived from Hydride-Meisenheimer complex became more pronounced during the incubation period, which was determined quantitatively. High-pressure liquid chromatography was used to measure residual TNT, which also resolved the metabolic intermediates (i.e., 2,4-dinitrotoluene, 2,6-dinitrotoluene and 2,4-dinitro-6-hydroxytoluene). Gas chromatography-mass spectrometry was used to verify these intermediates. Quantification of the nitroreductase (pnrB) gene isolated from S. maltophilia OK-5 growing in pink water was performed with real-time PCR. The amount of pnrB gene copies increased to 10³-fold after 5 days of incubation time.

쇠퇴지방도시 유형화와 재생과제

조수희(Cho, Su Hee),정재호(Chung, Jae Ho) 한국부동산학회 2010 不動産學報 Vol.40 No.-

In this study, 50 cities except for capital region were defined as the provincial cities, and the declining cities among theses cities were classified. And we examined the declining area and internal and external causes of the decline . (2) RESEARCH METHOD Our research has been done with the literature review on thoory and previous research. Furthermore, quantitative statistics, factor analysis, and discriminant analysis were conducted using SPSS 12.0 package. 1n addition, a survey of 165 government officers in charge of urban redevelopment, urban planning, and housing redevelopment in the declining provincial cities was conducted, and the hypothesis was examined through x2-Test and ANOVA-Test. (3) RESEARCH FINDINGS Research results show that 11 declining provincial cities including Jungeub city, Mungyoong city, and Sangzhou city, had been formalized. The internal causes of the decline and the vision of city were similar. but external causes were different. 2. RESULTS We classified the declining cities of 50 provincial cities and found the characteristics. Next we examined the hypothesis and proposed several solutions. We found the declining areas were centered around the city. To prevent the decline, we should strive for job creation and population inflow. Also, the population inflow of metropolitan areas should be prevented, Lastly, the city must have a vision for growth and development.

여고생의 영양지식, 식태도, 식습관 및 식품영양표시에 대한 인식

조수희(Su Hee Cho),유현희(Hyeon Hee Yu) 대한지역사회영양학회 2007 대한지역사회영양학회지 Vol.12 No.5

This study was carried out to investigate the nutrition knowledge, dietary attitudes, and dietary habits of girl`s high school students in Kunsan, and to investigate their recognition of food-nutrition labeling. The results are summarized as follows. General nutrition knowledge is relatively low, with an average of 0.57. It was shown that high school female students skipped breakfast rather than lunch or dinner, with a skipping rate of 28.9% for breakfast, 0.7% for lunch, and 8.6% for dinner. Regarding snacks, 35.5% of all the surveyed students had 1 snack per day, with 31.9% having them between lunch and dinner. The most popular snacks include biscuits (22.3%), noodles (18.3%) and bakery (13.3%). The most popular response was that students ``sometimes checked`` the food label of processed domestic and imported processed food. The level of satisfaction with food labels is moderate, with an average of 2.96, out of 5. The most satisfactory title about food labels was ``helpful for food selection`` with 3.19. On the other hand, the least satisfactory title was ``understands the label`` with 2.78. Regarding the identification of the nutrition labeling, the highest response was ``sometimes watched, sometimes not,`` with 40.5%. Products which were most often checked were milk/milk products (3.44), snacks/bread (3.33), and soft drinks (3.07). Among nutrition labeling items, total calories was the most important, followed by fat, carbohydrate, cholesterol and calcium. The question regarding the knowledge of nutrition labeling rated an average of 0.58 (out of 1). There was a significant positive correlation between the degree of the nutrition label verification and the dietary attitude score, along with the nutrition labeling knowledge and the nutrition knowledge score of the subjects. On the other hand, the degree of the nutrition label verification and the knowledge on nutrition labeling had a significant negative correlation. Hence, it is of the opinion that education on properly reading nutrient information is necessary to enable adolescents to apply that in real life. Furthermore, labeling nutritional information on processed food through a more comprehensive method is deemed necessary as a supporting measure. (Korean J Community Nutrition 12(5) : 519~533, 2007)

유류누출 지역에서 유래한 토착세균, Pseudoalteromonas sp. HK-3 배양에서 생물계면활성제의 최적 생산

조수희(Su-Hee Cho),마채우(Chae-Woo Ma),오계현(Kye-Heon Oh) 한국생물공학회 2011 KSBB Journal Vol.26 No.1

The principal objective of this study was to determine the optimal conditions for the production of biosurfactant by the indigenous bacterium, Pseudoalteromonas sp. HK-3, originating from oil-spilled areas. The relationship between total biosurfactant production and the factors affecting biosurfactant production were evaluated by statistical analysis using SPSS software. The effects of various supplemental carbon sources (e.g., glucose, dextrose, mannitol, citrate, acetate) on the maximal production of biosurfactant by the test culture of Pseudoalteromonas sp. HK-3 was then evaluated. As a result, mannitol was found in this study to be the best supplemental carbon source for the production of biosurfactant. A spot inoculation of crude cultural liquid containing the HK-3 cells generated the largest clear zone, whereas only small clear zones appeared around the spots inoculated with either supernatant only or cell pellets following centrifugation. Our results demonstrated that the HK-3 test culture supplemented with 2% mannitol at an initial pH of 6 generated the maximal amount of biosurfactant within 72 h of incubation.

기업의 문화예술지원과 경영성과 및 가치와의 상관관계 연구

조수희(Su-Hee Cho),김세준(Sae-June Kim) 대한경영학회 2014 大韓經營學會誌 Vol.27 No.8

도시성장과 쇠퇴로 본 충청권 지역유형화와 도시재생 방향

임준홍(Lim, June Hong),조수희(Cho, Su Hee),김용범(Kim, Yong Beom) 한국부동산학회 2010 不動産學報 Vol.40 No.-

1. CONTENTS (1) RESEARCH OBJECTIVES This study was carried out to set UP the toward of urban regeneration through the analysis for characteristics of declining cities about Chung-chong provmce. (2) RESEARCH METHOD Physical variables which represent the attitude survey of experts and urban level were used to analyze their characteristics and each cities were di1iided into types by their characteristics, and then proposed the policy of urban regeneration. (3) RESEARCH FINDINGS The results showed that the cities of Chung-chong province are stagnant and being declined except a neighboring area to the capital city and the Seoul-Busan expressway. In especially, most of their center are stagnant and being declined. 2. RESULTS They were divided into each types by cluster analysis am the policy of their urban regeneration follows: First cluster needs to create jobs, regenerate economy and their community. Second, it needs to regenerate their center am a new growth engines. Third, it needs the regeneration of city center, new town am the division of the roles of the center and to seek ways to differentiate government supports for city regeneration. The growing cities needs the development way of immovables using deregulation and private vitality and the depressed and declined cities needs the financial resources and constant supports.

결핵균과 비결핵성항산균 검출에 Real-time PCR의 유용성

윤은영 ( Eun Young Yun ),조수희 ( Su Hee Cho ),고세일 ( Se Il Go ),백종하 ( Jong Ha Baek ),김유은 ( You Eun Kim ),마정은 ( Jeong Eun Ma ),이기동 ( Gi Dong Lee ),조유지 ( Yu Ji Cho ),정이영 ( Yi Yeong Jeong ),김호철 ( Ho Cheol Kim 대한결핵 및 호흡기학회 2010 Tuberculosis and Respiratory Diseases Vol.69 No.4

Background: The purpose of this study was to evaluate recently developed real-time polymerase chain reaction (PCR) assay kit to detect Mycobacterium tuberculosis (MTB) and nontuberculous mycobacteria (NTM) in respiratory specimens. Methods: We assessed the positive rate of the real-time PCR assay to detect MTB and NTM in 87 culture-positive specimens (37 sputum, 50 bronchial washing), which were performed real-time PCR by using Real-QTM MTB&NTM Kit from January 2009 to June 2009, at Gyeongsang University Hospital. To compare the efficacy with the TB-PCR assay, we evaluated 63 culture-positive specimens (19 sputum, 44 bronchial washing) for MTB or NTM, which were performed TB-PCR by using ABSOLUTETM MTB II PCR Kit from March 2008 to August 2008. Results: Among 87 specimens tested using real-time PCR, MTB and NTM were cultured in 58 and 29, respectively. The positive rate of real-time PCR assay to detect MTB was 71% (22/31) and 92.6% (25/27) in AFB stain-negative and stain-positive specimens. For NTM, the positive rate of real-time PCR was 11.1% (2/18) and 72.7% (8/11) in AFB stain-negative and stain-positive specimens. Among 63 specimens performed using TB-PCR, MTB and NTM were cultured in 46 and 17, respectively. The positive rate of TB-PCR was 61.7% (21/34) and 100% (12/12) in AFB stain-negative and stain-positive specimens. TB-PCR was negative in all NTM-cultured 17 specimens. Conclusion: TB/NTM real-time PCR assay is useful to differentiate MTB and NTM in AFB stain-positive respiratory specimens and it is as effective in detecting MTB with TB-PCR.

건조표고 버섯의 침지조건에 따른 수화양상

조수희,조은자 성신여자대학교 생활문화연구소 2001 生活文化硏究 Vol.15 No.-

Dried pyogo mushrooms(Lentinus edodes) rehydrated under various condition. In this study was investigated the hydration aspect of the dried Lentinus edodes that differed drying temperature while soaked in distilled water at 4±1℃, 15±2℃, 40±2℃, 60±2℃. This study was compared with the properties of hydration while soaked in distilled water, 0.5M NaCl solution and 0.5M sucrose solution at the temperature. This study also investigated the effects of drying temperature on hydration of the dried Lentinus edodes. Results were as follows. 1. The average moisture content of fresh Lentinus edodes was 80.0% and those of natural dried sample, hot air dried sample at 40℃ and hot air dried sample at 60℃ were 8.2%, 8.2% and 7.9% respectively. 2. The amount of water uptake of dried Lentinus edodes soaked in distilled water was increased as soaking temperatures get higher. Water uptake of all samples soaked at 40±2℃ and 60±2℃ increased rapidly at initial soaking and then slow as soaking time prolonged. the water absortion rate of natural dried sample and hot air dried sample at 60℃ soaked at 60±2℃ was the highest. 3. Water uptake rate constant(K) of natural dried sample dried sample soaked for 60 minutes at 15±2℃, was the highest by 0.7304. The amount of water gain of all the dried samples showed a linear relation with the square root of soaking time regardless of soaking temperature. Activation energy of water uptake of natural dried sample, hot air dried sample at 40℃ and hot air dried sample at 60℃ soaked at 40~60℃ was greater than that of at 15~40℃ by 5.5, 6.5, 2.5 times respectively. 4. Water uptake rate constant(K) of natural dried sample, hot air dried sample at 40℃ and hot air dried sample at 60℃ was the lowest in 0.5M sucrose solution to 60 minutes of soaking times by 0.319., 0.5806, 0.2352 respectively. All of them take 300 minutes over of soaking times to reach equilibrium water content state.