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        디펩타이드의 B16 악성흑색종세포에서 멜라닌 생성억제작용

        남희승 ( Hee Seung Nam ),김은현 ( Eun-hyun Kim ),김수연 ( Su Yeon Kim ),이현이 ( Hyun-e Lee ),홍지연 ( Jiyun Hong ),이재국 ( Jae Guk Lee ),조성태 ( Sung Tai Cho ),조양환 ( Yang Hwan Cho ),윤혜영 ( Hye-young Yun ),백광진 ( Kwang Ji 대한화장품학회 2012 대한화장품학회지 Vol.38 No.1

        본 연구에서는 B16 악성흑색종 세포에서 디펩타이드(dipeptide)의 멜라닌생성 저해 효과를 연구하였다. 실험결과 WV (트립토판-발린), WM (트립토판-메치오닌), CQ (시스테인-글루타민)는 멜라닌 생성을 농도 의존적으로 감소시켰다. 그러나 디펩타이드는 멜라닌 생합성과정의 속도 조절 단계 효소인 타이로시네이즈(tyrosinase)의 활성을 직접 감소시키지는 않았다. 따라서 타이로시네이즈의 발현양상을 조사하였고, 실험 결과 α-MSH가 유도한 타이로시네이즈 발현이 WV, WM, 그리고 CQ에 의해 억제되었다. 그러므로 WV, WM, 그리고 CQ가 타이로시네이즈의 억제성 조절(down-regulation)을 통해 멜라닌 생성을 감소시킨다고 제안될 수 있다. In the present study, we investigated the effects of dipeptides on melanogenesis in B16 melanoma cells. It was found that WV (Trp+Val), WM (Trp+Met), and CQ (Cys+Gln) decreased melanin production dosedependently. However, dipeptides did not directly inhibit tyrosinase activity, the rate-limiting melanogenic enzyme. Therefore, we further investigated the expression of tyrosinase. Our results showed that α-MSH-induced tyrosinase expression was down-regulated by WV, WM, and CQ. Thus, we propose that WV, WM, and CQ show hypopigmentary activity through tyrosinase down-regulation.

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        동백씨 추출물이 세포노화 및 사이토카인과 Nitric oxide 생성에 미치는 영향

        조성태,박재승 한국미용학회 2001 한국미용학회지 Vol.7 No.1

        The present study was undertaken in an effort to explore the effect of Camellia seed extract treatment on the production of IL-1β, TNF-α, IL-6 and IL-12 in mice by LPS. In addition, effect of Camellia seed extract on LPS-and IFN-γ-induced nitric oxide production by peritoneal macrophages. Mice were treated by a single intraperitoneal injection of Camellia seed extract 1, 5, and 10mg per mouse on 1hr before LPS injection or peritoneal cells harvested, It was found that Camellia sued extract treatment inhibited the production of IL-1β, TNF-α, IL-6, and IL-12 but, a small amount of nitric oxide production compared with control mice. Taken together, this study may provide the direct evidences that Camellia seed extract may inhibit the production of IL-1β, TNF-α, IL-6, and IL-12 bin, a small enhanced of nitric oxide production. And suggest that production of cytokines and nitric oxide in direct or indirect related to cell aging by various mechanisms.

      • 韓國美容産業의 現況과 專門美容學徒의 健康增進 行態에 관한 關聯要因分析

        조성태 광주보건대학 1998 論文集 Vol.23 No.-

        From March 1, 1998 through May 31, this questionnaire was made by 242 students who specialized in department of beauty related. It was for helping to devise a policy and to develop a program for student's health promotion by understanding the consciousness behavior level of student in our country and the related factors which are under the influene are as follows; 1. In health promotion score level by related factors, the health diagnosis level was 7.37(81.9/100) of the perfect score 9, nutrition level was 7.00(77.8/100), health education behavior level was 6.00(66.7/100), exercise behavior level was 6.01(66.8/100), health knowledge level was 6.00(66.7/100). 2. Health diagnosis behavior level was significantly high when age is older, when the economic status is better. And when satisfied degree for vocational aptitude, educational environment, and the education contents. 3. Nutrition (dietary habit) status level was high in the age group of 26 over, in the upper economic classes(P<0.001). 4. Health education behavior level was high in the older group(P<0.001). Exercise behavior level was high in the better economic classes(P<0.01). 5. Knowledge level on health was high in the older age group, in the group of having a spouse, and in the residents living not in a large city(P<0.01). 6. When health status was higher, health promotion behavior, behavior level, health diagnosis(P<0.001), nutrition(P<0.05), health education behavior(P<0.05). exercise behavior(P<0.01) and the knowledge level on health was high. 7. The main factors which are under the influence on the degree of practicing healthy life were the level of knowledge and behaivor, health status, and the satisfied degree of educational environment. These variables could explain it 18.0% 8. The factors which are under the influence on health promotion behavior and behavior levels were the variables of the satisfied degree of education contents, health knowledge, economic status, health status. These variables could explain it 21.3%. By these results, it is important for student's health promotion to level up the health diagnosis behavior, dietary habit considering nutrition, behavior on health education, behaivor for exercise, and knowledge on health. Especially we should develop the proper program considered health status, satisfied degree of working environment and education contents, knowledge on health, and behavior level. Key word : Health Promotion, Behaivor, Health diagnosis.

      • 일광차단제 효과에 의한 흰쥐의 표피변화

        조성태 광주보건대학 1996 論文集 Vol.21 No.-

        The investigation of the change in Sprague-Dawley Rat skin in process of time, being irradiated by UVA(2 joul/㎠) and UVB(50 joul/㎠), some areas were applied with sunscreen (SPF 4. 15. 34) and one was applied nothing, gave me the results as follows ; 1. UVBs were more remarkable than UVAs in the change symptoms of skin structure such as hyperkeratosis, parakeratosis, hypergranubsis and hypertrophy. 2. SPF 4 areas were more similar to the unapplied one in the change of skin structure than UVAs. 3. SPF 15 areas were noticed slight hyperkeratosis at 28th in UVAs, while UVBs showed the same symptom at 7th. 4. SPF 34 areas were noticed nothing in UVAs and UVBs. From the above results I conclude that the change of rat skin according to irradiation UVB was more remarkable than UVA in the areas of applied nothing The effective usage of sunscreen is resulted by applying sunscreens of higher-grade than SPF 15.

      • 사과의 저온저장 중 superoxide dismutase 활성의 변화

        조성태,권태영 광주보건대학 2000 論文集 Vol.25 No.-

        We are in incestigated the water content, total sugar content, titratable acicidity, polyphenol content, and superoxide activities of 3 apple culfivars (Jonathan, Ralls Janet, and Fuji) at 5℃ for 6 months. For the index of storage stability such as the content of water andt total sugar, Ralls Janet was the most excelleut among 3 Cultivars, and its polyphenol content and SOD activity Were more higher. The total SOD acticity of Ralls increased from 186units/gfw at initial of storage to 318units/gfw after 6 month. Also, SOD activity of Fuji increased from 137units/gfw to 155units/gfw. However, SOD activity of Jonathan was decreaed from 185units/grw to 136units/gfw. The study strongly suggested that SOD activity was involved in storage. It is likely taht this result affect a study in protection of skin and hair and the beauty.

      • VIDAL SASSOON과 TONI & GUY의 커트기법과 특성의 차이점에 관한 비교연구

        지상기,조성태 공주영상정보대학 1999 논문집 Vol.6 No.-

        At present, our society have encountered a variety of changes under the course of getting into a developed county. To cope with the advancement, the creation of women's hair style also should be emphasized based on a scientific and geometrical process and which could be an effective-coreation meeting the current changing society. Firstly, accurate importation of cutting system based on academic ground. Secondly, creation of healthy hair-quality for active life style matching with individual subject. Thirdly, Impdrtion of strong professionalism that can help improve technical knowledge and sensitiveness. Thus, we under our internal necessity, have to develope our beauty science through a aggressive study of the differences and characteristic of two(2) leading groups in the (lewd of hair science. The study and method will be to find out the origination of specific and scientific skill through the historical and contemporary social background and to compare and analyze characteristic of cutting skill against actually completed hair-styles. In fact the characteristic differences between two groups will be a matter of cutting skill/namely, while the VIDAL-SASSOON is pursuing a simple and formative style based on line-beauty, TONI & GUY is emphasizing on italian characteristic sensitiveness subjected on texturized avant-garde creation. The blunt cutting itself is almost similar in the basic technique but a big deference exists in the process of making a sihouette. And further, the lineal beauty demonstrates a strong unisexual feeling, the curbed silhouette beauty reflects a feminine touch. in conclusion VIDAL-SASSOON does emphasize a sharp line and TONI & GUY is emphasizing flexible curved beauty added by hair-dressing. As foregoing statement indicates. We have to accept the fact that the two groups have accomplished a geometrical and scientific cutting hair-style through basical ground and further create a logical hair-style in the coaming 21st century.

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