동물에서 Coxiella burnetii 항체를 진단하기 위한 경쟁효소면역법 개발

조동희,김용주,위성환,조미영,권창희,강영배,박용호,조상래,Cho, Dong-hee,Kim, Yong-ju,Wee, Sung-hwan,Cho, Mi-young,Kweon, Chang-hee,Kang, Yung-bai,Park, Yong-ho,Cho, Sang-nae 대한수의학회 2000 大韓獸醫學會誌 Vol.40 No.1

Coxiella burnetii (C burnetii) is the causative agent of Q fever in animal and human. The distribution of the disease has been documented around world. In this study we developed the competitive enzyme linked immunosorbent assay(cELISA) and compared it with indirect immunofluorescent assay(IFA). A monoclonal antibody(Mab) against C burnetii and a peroxidase-conjugated anti-mouse IgM were used as an indicator system competing against antibody in animal serum or as an indicater of the absence of antibody. Sera were considered antibody positive when the percentage inhibition index(PI index) is upper than 30. PI index is calculated as 100-[sample OD/Mab OD)${\times}100$]. Among 162 bovine serum samples, 23 samples were antibody positive both in cELISA and IFA. And 156 samples showed same results. From goat with experimentally induced infection with C burnetii the antibody was detected 20 days early in cELISA compared to IFA. On the basis of present findings, it was demonstrated that cELISA is a reliable diagnostic method for The detection of specific antibodies against C burnetii infection.

위암에서 $PPAR-{\gamma}$ 발현의 임상적 의의

조동희,강성구,박상수,윤진,김일명,윤성민,이윤경,이용직,양대현,조익행,신동규,Cho, Dong-Hui,Kang, Sung-Gu,Park, Sang-Su,Yoon, Jin,Kim, Il-Myung,Yoon, Seong-Min,Lee, Yun-Kyung,Lee, Yong-Jik,Yang, Dae-Hyun,Cho, Ik-Hang,Shin, Dong-Gue 대한위암학회 2006 대한위암학회지 Vol.6 No.4

목적: 최근 Peroxisome-proliferator-activated receptors (PPAR) 의 역할에 대한 관심이 고조되고 있으나 위암에 대한 실제 임상연구는 부족한 실정이다. 이에 저자들은 위암 조직에서의 $PPAR-{\gamma}$의 발현율과 임상적 의의에 대해 분석을 시행하였다. 대상 및 방법: 2001년 1월부터 2005년 12월까지 서울의료원에서 위암으로 근치적수술을 시행 받은 128명을 대상으로 하였다. 적출된 위조직으로 만들어진 파라핀 블록을 이용해 위암조직 125예와 위암주변 정상조직 128예를 채취하여 $PPAR-{\gamma}$ 발현을 면역조직화학염색으로 확인한 후 비교 분석하였다. 결과: 대상 환자의 평균 연령은 61세였으며 남자 84명 (65.6%), 여자 44명(34.4%)으로 1.9 : 1이었다. $PPAR-{\gamma}$ 발현의 양성률은 정상조직 73/128명(57.0%)에 비하여 위암조직에서는 104/128명(81.3%)으로 유의하게 높았다(P<0.001). 위암조직의 분화도에 따른 $PPAR-{\gamma}$ 발현의 차이에서 분화도가 양호한 경우의 양성률(87.0%)에 비해 나쁜 경우 발현율(74.6%)이 낮았으나 통계적 유의성은 없었다(P=0.074). 위암조직에서와 $PPAR-{\gamma}$ 발현 유무와 생존율 사이에 통계적 유의성은 없었으나(P=0.377) 정상조직에서 $PPAR-{\gamma}$의 발현은 단변량 분석에서 유의하게 높은 생존율을 보였다(P=0.003). 5년 생존율과 연관지어 다변량 분석 통계에서 의미가 있었던 것은 UICC TNM 병기뿐이었다(P<0.001). TNM 병기별 5년 생존율은 IA-89.9%, IB-90.0%, II-89.7%, IIIA-41.0%, IIIB-25.9%, IV-14.6% 였다. 결론: 위암조직에서 정상조직에 비하여 $PPAR-{\gamma}$ 발현이 유의하게 높았으며 위암조직의 분화도가 좋을수록 발현율이 높았다. $PPAR-{\gamma}$의 정상조직 내 발현이 단변량 분석상 유의한 생존율 향상이 있었지만 다변량 분석에서는 유의성이 없어 향후 이에 대한 좀더 많은 증례로 유의성을 찾는 연구가 필요할 것으로 생각된다. Purpose: Recently, interest in peroxisome-proliferator-activated receptors (PPAR) has increased, although clinical studies of the effect of $PPAR-{\gamma}$ expression on gastric cancer have not been reported yet. In this study, we investigated the role of $PPAR-{\gamma}$ expression in gastric cancer patients. Materials and Methods: One hundred twenty-eight (128) samples of both gastric cancer and normal tissues were obtained from 128 patients who had undergone at a curative gastrectomy at Seoul Medical Center from Jan. 2001 to Dec. 2005. $PPAR-{\gamma}$ expression was determined by using immunohistochemical staining, and the results were analyzed. The statistical analysis was based on clinicopathological findings and the differences in survival rates. Results: The mean age of the patients was 6n, and the male : female ratio was 1.9 : 1. $PPAR-{\gamma}$ expression was significantly higher in cancer tissues than in normal tissue (81.3% vs. 57.0%, p<0.001). There was insignificant difference between well and moderately differentiated types and poorly differentiated types in terms of the expression of $PPAR-{\gamma}$ (87.0% vs. 74.6%, P=0.074). In the univariate analysis the survival rate was significantly increased when $PPAR-{\gamma}$ was expressed in normal tissue (P=0.003). In the multivariate analysis, only the UICC TNM staging had significance related to the survival rate. Conclusion: The rate of $PPAR-{\gamma}$ expression was higher in cancer tissue than it was in normal tissue from gastric cancer patients. In the univariate analysis, $PPAR-{\gamma}$ expression in normal tissue had significance with respect to survival, but the multivariate analysis showed no such significance. Thus, we should further evaluate more cases to determine whether or not such a significance exists.

정천화담강기탕가녹용(定喘化痰降氣湯加鹿茸)이 Xylene으로 유발된 마우스의 급성 염증에 미치는 영향

조동희,손지영,이연경,최규호,박미연,최해윤,김종대,Cho, Dong-Hee,Son, Ji-Young,Lee, Yeon-Kyeong,Choi, Kyu-Ho,Park, Mee-Yeon,Choi, Hae-Yun,Kim, Jong-Dae 대한한의학방제학회 2007 大韓韓醫學方劑學會誌 Vol.15 No.2

In the present study, the anti-inflammatory effect of "Jungcheonhwadamgangki-tang ga Antler" water extract was tested in Xylene-Application mouse ear acute inflammation model. The test articles were once dosed before Xylene-Application, and the changes on body weight and ear weights and histopathological observation of induced ear were conducted with ear histomorphometry. The obtained results were as follows. The increases of absolute and relative ear weight detected in vehicle control compared to that of sham, were significantly and dose-dependently inhibited by Jungcheonhwadamgangki-tang ga Antler in the present study. A classic acute inflammatory histological changes such as subcutaneous edema, thickness and infiltration of inflammatory cells, was detected in vehicle control. However, these histological changes were significantly and dose-dependently inhibited by Jungcheonhwadamgangki-tang ga Antler. In addition, the increases of ear thickness half and thickness full detected in the vehicle control were also dose-dependently decreased in the all Jungcheonhwadamgangki-tang ga Antler-dosing groups. Base on these results, it is concluded that Jungcheonhwadamgangki-tang ga Antler extracts has clear anti-inflammatory effect on the acute inflammation such as bronchial asthma.

학술발표회 초록 : ( 분리기술 Poster 발표 ) ; 다성분 혼합물의 흡착평형 데이타 예측 모델에 관한 연구

조동희,양재영,조찬휘,이한주 ( Dong Hee Cho,Jae Young Yang,Chan Hwi Cho,Han Ju Lee ) 한국화학공학회 1994 추계학술발표회 Vol.- No.-

마황윤폐탕(麻黃潤肺湯) 추출물의 수컷 SD Rats에서 경구 단회투여 독성 평가

조동희,박미연,최해윤,김종대,전귀옥,Cho, Dong-Hee,Park, Mee-Yeon,Choi, Hae-Yun,Kim, Jong-Dae,Jeon, Kwi-Ok 대한한방내과학회 2006 大韓韓方內科學會誌 Vol.27 No.1

Objectives & Methods : To obtain the 50% lethal dose(LD50), approximated lethal dose(ALD) and approximated target organs of 'Mahwangyounpae-tang' for further study into such things as repeated dose toxicity, genotoxicity and reproductive toxicity, single dose toxicity was tested in male SD rats according to KFDA Guideline 1999-61[KFDA, 1999] at dosage levels of 2,000, 1,000, 500, 250 and 125 mg/kg/$10m{\ell}$. In this study, mortalities, clinical signs, body weight changes and body weight gains, gross findings and weight of principal organs were detected during and/or after 14 days of single dosing. Results & Conclusions : After 2 or 3 days of dosing, 1 or 2 animals in 2,000 mg/kg-dosing groups died. Excitation and leaping response were observed as test article-treatment related clinical signs. These abnormal signs were restricted to 2,000 and 1,000 mg/kg-dosing groups and survivors recovered to normal within 3 or 4 days after dosing. Significant decrease in body weight were observed in some periods of observation in 2,000 and 1,000 mg/kg-dosing group, from 1 days after dosing compared to those of vehicle control group. Significantly diminished body weight gains were observed in observation periods in 2,000 and 1,000 mg/kg-dosing group compared to those of vehicle control group. Hypertrophy and hemorrhage of heart and decoloration of kidney were observed as test article-treatment related gross findings. These abnormal findings were restricted to 2,000 and 1,000 mg/kg-dosing groups. A significant increase of absolute and relative heart and kidney weight were demonstrated in 2,000 mg/kg-dosing groups. The value for LD50 found in this study was 2,218.57 mg/kg. ALD in this study was 2,000 mg/kg, and the target organs are considered to be the heart and the kidney.

증례 : 호흡기 ; 비특이적 간질성 폐렴의 양상으로 발생한 IgG4 연관 폐 질환의 1예

조동희 ( Dong Hui Cho ),안지환 ( Ji Hwan An ),강유미 ( Yu Mi Kang ),채은진 ( Eun Jin Chae ),송준선 ( Joon Seon Song ),송진우 ( Jin Woo Song ) 대한내과학회 2015 대한내과학회지 Vol.88 No.3

특발성 비특이적 간질성 폐 질환과 IgG4 related lung disease는 증상, 폐기능 검사, 영상의학 소견만으로는 감별이 어려운 질환이다. 하지만 IgG4 related lung disease는 steroid 치료에 좋은 치료 반응을 보이는 것으로 알려져 있어 이들의 감별은 매우 중요하다고 할 수 있다. 본 증례는 특발성 간질성 폐 질환, 특히 비특이적 간질성 폐렴에서 혈청 IgG4의 증가 혹은 조직 소견상 lymphoplasmacytic infiltration이 다수 관찰되고 obliterative phlebitis, storiform fibrosis 등이 관찰된다면 IgG4 연관 질환의 폐 침범 가능성을 고려해야 함을 시사해 준다. Immunoglobulin (Ig) G4-related disease is a recently described systemic inflammatory disease characterized by high serum IgG4 concentrations and sclerosing inflammation of numerous IgG4-positive plasma cells that responds favorably to steroid treatment. Although initial description of this disorder focused on its pancreatic presentation, it has become apparent that it is a systemic disease. In this report, we describe a case of IgG4-related lung disease presenting as non-specific interstitial pneumonia in a 78-year-old male with interstitial lung disease. Pathological examination through video-assisted thoracic surgery showed a non-specific interstitial pneumonia pattern and numerous (> 50/high-power field) infiltrating IgG4-positive plasma cells. Laboratory tests also revealed a high serum IgG4 concentration. Prednisolone therapy was initiated and his symptoms and reticular opacity improved after two months of treatment.(Korean J Med 2015;88:308-312)

증례(證例) : 한방 치료 후 폐기능이 개선된 폐기종 환자 증례보고

조동희 ( Dong He Cho ),손지영 ( Ji Young Son ),박미연 ( Mee Yeon Park ),최해윤 ( Hae Yun Choi ),김종대 ( Jong Dae Kim ) 대구한의대학교 제한동의학술원 2008 東西醫學 Vol.33 No.3

인공지능 스피커의 사용자 만족이 지속적 사용의도에 미치는 영향 요인 - 감정적 애착의 매개효과를 중심으로 -

조동희 ( Dong-hee Cho ),이연준 ( Youn-joon Lee ) 한국디자인트렌드학회 2019 한국디자인포럼 Vol.24 No.2

연구배경 4차 산업혁명과 함께 핵심 산업으로 인공지능이 대두되고 있다. 그중 핵심기기로 인공지능 스피커가 주목받으며 홈 IoT 시장에서 빠른 속도로 성장하고 있으나 이러한 성장세와는 달리 인공지능 스피커의 사용자 만족도는 낮다. 이러한 사용자의 불만족은 결국 사용자가 해당 제품의 사용을 중단하게 되는 중요한 요인으로 작용하고 있다. 연구방법 본 연구에서는 인공지능 스피커의 지속적 성장 및 확산을 위하여 국내 인공지능 스피커 제품을 대상으로 사용자의 지속적 사용의도에 영향을 주는 요인에 관해 연구하였다. 연구목적을 달성하기 위해, 지속적 사용의도와 관련된 선행연구를 통해 사용자 만족 중심의 이론과 사용자 경험의 감정 요인으로 감정적 애착의 선행요인들이 인공지능 스피커의 지속적 사용의도에 어떠한 영향을 미치는지 살펴보았다. 또한, 감정적 애착이 사용자 만족과 지속적 사용의도 간에 매개효과를 하는지 확인하였다. 연구결과 인공지능 스피커의 사용자 만족이 지속적 사용의도에 유의미한 영향을 주며, 사용자 만족이 지속적 사용의도에 미치는 영향을 감정적 애착이 부분매개를 하는 것으로 나타났다. 결론 인공지능 스피커 사용자의 지속적 사용의도 향상을 위해 미흡한 기능 개선과 사용 편의성 향상을 통한 사용자 만족 향상도 중요하지만, 사용자가 자신만의 공간과 조화를 이룰 수 있는 상품을 선택하고 변경할 수 있도록 사용자의 라이프스타일(lifestyle)에 맞춘 디자인과 커스터마이제이션(customization)이 가능하도록 개발되어 인공지능 스피커에 대한 애착을 향상시켜야 할 필요가 있다. Background With 4th Industrial Revolution, AI (Artificial Intelligence) has emerged as a major industry. Among variety products that are developed using the AI technology, AI speaker is gaining attention as a major product and shows rapid growth in consumer IoT (Internet of Things) market. Unlike the rapid growth and attention in the market, consumer satisfaction of AI speaker is low. Low satisfaction level is the important factor to ultimately end the usage of AI speaker. Methods In this study with the hope of securing continuous growth and the spread of the AI speaker, factors that would affect the user’s continuous usage of the AI speaker usage have been explored. To achieve research objectives, we looked at the impact of emotional attachment as an emotional source of emotional attachment as an emotional factor in user satisfaction theory and in prior research related to continuous use. It also confirmed that emotional attachment also mediated between user satisfaction and continued use. Result The research result shows that satisfaction level does affect the usage continuity; furthermore, users’emotional factors are identified as important variables to allow users decision to use AI speakers continuously. Conclusion This shows that AI speakers to have an ability to be customized to create unity with user’s personal space is as important as the development of AI speaker technology to increase the user’s satisfaction level and to increase the consumers’ usage continuity. Thus, by having AI speakers’ design to fit into user’s lifestyle, user’s emotional attachment toward the product will increase, ultimately increasing the continuity of the product usage.

해양 유류 유출 사고를 대비한 서로게이트 모델 기반의 방제 자원 최적 배치

조동희(Dong-Hee Cho),김혜진(Hye-Jin Kim),김용혁(Yong-Hyuk Kim) 한국해양환경·에너지학회 2020 한국해양환경·에너지학회 학술대회논문집 Vol.2020 No.7

재발성 급성 췌장염의 진단과 치료

조동희 ( Dong Hui Cho ),송태준 ( Tae Jun Song ) 대한췌장담도학회 2016 대한췌담도학회지 Vol.21 No.1

Acute recurrent pancreatitis (ARP) refers to a clinical condition characterized by repeated episodes of acute pancreatitis, diagnosed retrospectively after at least the second episode of acute pancreatitis. It is still controversial that acute pancreatitis can progress to chronic pancreatitis, and acute, acute recurrent and chronic pancreatitis is a continuum of disease. The causes of ARP can be divided into mechanical, hereditary and metabolic factor. Despite recent advances in diagnostic technologies, the etiology of ARP still remains unknown in up to 30% of cases. Especially in recurrent episode of idiopathic pancreatitis, a clinician should be considered not only the common causes of ARP, such as gallstone disease and alcohol, but also rare causes of ARP. The common causes of ‘idiopathic’ recurrent pancreatitis are microlithiasis, sludge, sphincter of oddi dysfunction, pancreas divisum and hereditary pancreatitis. Various treatment options, such as cholecystectomy, endoscopic sphincterotomy, medical and surgery can be applied according to the identified etiology of ARP and treatment should be individualized. Currently, endoscopic treatment is increasingly performed and served as a curative treatment strategy. The medical treatment can be an option in microlithiasis and sludge, but it has limitation in terms of systemic side effect, efficacy and lack of long term outcome. Endoscopic treatment should be considered in selected patients with identifiable cause, and post procedural complication should be considered before endoscopic treatment.