광합성세균의 파이롯트 단위 생산을 위한 고순도 배양

조경숙,임태빈,정해윤,조정섭,김중균,Cho, Kyoung Sook,Yim, Tae Bin,Jeong, Hae Yoon,Cho, Jeong Sub,Kim, Joong Kyun 한국해양바이오학회 2006 한국해양바이오학회지 Vol.1 No.4

오염된 양식 해수의 수질정화능력이 뛰어난 광합성세균의 고순도 대량배양을 위한 실험을 실험실 단위 및 파이롯트 단위에서 수행했다. Gas pack 반응기, flask 반응기 및 N-source로서 질소 가스를 사용한 실험 결과, 광합성세균은 현미경상 약 90%이상의 순도를 나타내었고, CFU 측정 결과에서도 붉은색을 띄는 광합성세균 colony만을 관찰 할 수 있었다. 배지의 교차에 의한 배양실험에서 기초배지를 이용한 배양이 광합성세균의 성장에 가장 효과적임을 알 수 있었다. 이러한 배양조건하에서의 5L bioreactor 배양에서 비증식속도가 $0.18h^{-1}$로 나타났고, 실험실 단위의 결과를 이용한 pilot 단위에서의 배양결과, 실험실 단위의 비증식속도와 동일한 값을 얻을 수 있었다. Lab-scale and pilot-scale productions of photosynthetic bacteria, that were able to efficiently treat wastewater from aquacultural farm, were experimented for their highly-pure culture. The results of experiments in a gas pack reactor, an anaerobic flask and a flask using $N_2$ gas as N-source showed that only photosynthetic bacteria formed red colonies on agar plate and their purity was over 90% in a colony, observed under a microscope. It was found that the basal medium could most promote the growth of photosynthetic bacteria, confirmed by experiments of serial cultures on various media. Under the culture conditions, the specific growth rate was found to be $0.18h^{-1}$ from the culture in 5L bioreactor and the same value could be obtained in pilot-scale production.

Alcaligenes latus 의 배양방법에 따른 생분해성 플라스틱의 생산특성

조경숙,최희식,류희욱,조강현,박성연 ( Kyung Suk Cho,Hee Sik Choi,Hee Wook Ryu,Kang Hyun Cho,Seong Yeon Park ) 한국환경생물학회 1998 환경생물 : 환경생물학회지 Vol.16 No.1

Optimum conditions for cell growth and poly (3-hydroxybutyrate, PHB) biosynthesis of Alcaligenes latus. which can accumulate PHB simultaneously in associated with cell growth and grows well on a cheap carbon source such as sucrose, were investigated. The effect of initial sucrose concentration, ranged from 10 to 50g/L, on the cell growth and PHB accumulation were studied by a batch culture in a 5 L fermentor. PHB productivity was increased from 0.11 to 0. 36 g-PHB/L·h with increasing sucrose concentration up to 30g/L, and it was fairly constant between concentrations of 30g/L and 50g/L. The maximum specific growth rate, 0.44h^-1, was obtained at 30 g-sucrose/L, and the growth was inhibited at concentrations of sucrose over 30g/L. A. latus was also cultivated to attain high PHB productivity by fed-batch or semicontinuous fed-batch culture techniques. PHB productivity by a fed-batch culture under phosphate limitation (1.14g-PHB/L·h) were smaller than that without nutrient limitation (2.10g-PHB/L·h), but there is no significant difference of PHB content (55-60%) obtained in both culture. By a semicontinuous fed-batch culture without nutrient limitation, high PHB productivity could be obtained as 4.5∼4.8 g-PHB/L·h. The average PHB content, the yield of dry cell weight, and the yield of per consumed sucrose were 60%, 0.48, and 0.32, respectively. These results suggest that the semi continuous fed-batch culture technique is one of promising methods to achieve high PHB productivity by A. latus.

통원 백내장 수술 후 개별 교육이 불안과 자가관리 수행에 미치는 영향

조경숙,노창래,Kyung-Sook Cho,Chang Rae Rho 대한안과학회 2012 대한안과학회지 Vol.53 No.3

Purpose: To investigate the influence of additional postoperative individualized teaching on the anxiety levels and self-care compliance of postoperative care in patients after one-day cataract surgery. Methods: The present study included 80 cataract patients who underwent phacoemulsification and posterior intraocular lens insertion performed by a single operator between January 2011 and February 2011. The control group consisted of patients who received postoperative education from an ophthalmology coordinator based on previous hospital policy. The experiment group consisted of patients who received additional individualized postoperative education from an operator in the recovery room. Trait anxiety on operation day, state anxiety on postoperative day 1, and self-care compliance and postoperative satisfaction at postoperative 1 month were evaluated. Results: There were no significant differences of trait anxiety levels on operation day or state anxiety on postoperative day 1 between the control and experiment groups. The experiment group revealed a higher score on the postoperative self-care compliance questionnaire, especially regarding care of the operation site. Conclusions: Individualized postoperative education increases self-care compliance level and contributes to improve postoperative recovery after one-day cataract surgery. J Korean Ophthalmol Soc 2012;53(3):412-419

국내 주요 일간지의 한방(보완)의료 관련 기사의 추세 분석

조경숙,박종구,김춘배,이선동,최서영,Cho Kyung-Sook,Park Jong-Ku,Kim Chun-Bae,Lee Sun-Dong,Choi Seo-Young 대한예방한의학회 2000 대한예방한의학회지 Vol.4 No.1

This study is conducted to evaluate the social concern of the related issues through trend analysis of reports on Oriental medical care in major domestic newspapers including Dong-a Ilbo, Hankook Ilbo, Chosunilbo, and JoongAng Ilbo. Materials of this study were collected from a total of 1,039 reports on Oriental medical care by internet search of each newspaper company's homepage from fan 1, 1993 to Dec 31, 1999. Two researchers analyzed the trend of report contents on Oriental medical care by publication year. The major results were as follows: The most frequent subject in these reports related to Oriental medical care was 'disease', appearing in 45.5% (473) of tile reports, followed by 'oriental health system (31.5%)' and "oriental medical facilities (16.5%)'. Also, topics on the collaborative efforts of Oriental and Western medical care were reported in 7.9% of the cases. According to a survey of the opinions held for the contents in these reports, 51.0% reports held neutral views while 48.2% and only 0.8% reports had favorable and negative views, respectively. In conclusion, the reports covering Oriental medical care in major domestic newspapers have increased in recent times and many held favorable views. Considering the widespread influence of mass-media, the government and health-related organizations must continuously monitor the opinions in daily newspapers, for the opinions are the influencing force of health-rotated behaviors and changes. In this light, these organizations should enforce the health policy on strategies for mutual development of Western and Oriental medicine in Korea.

광합성 세균을 포함한 고정화 복합미생물에 의한 질소성분 제거

조경숙,김정보,정수경,정해윤,조정섭,김중균,Cho, Kyoung Sook,Kim, Jeong Bo,Jeong, Soo Kyoung,Jeong, Hae Yoon,Cho, Jeong Sub,Kim, Joong Kyun 한국해양바이오학회 2006 한국해양바이오학회지 Vol.1 No.2

순환여과식 양어 생산시스템에 있어 발생되는 폐수의 효율적 처리를 위하여, 유독 질소성분 처리능력이 뛰어난 광합성세균(PSB)을 PVA에 고정화한 beads 또는 CTA에 고정화한 cubes와 $NH{_4}^+$를 잘 이용하는 균(AUB)을 고정화한 beads를 여러 비율로 혼합한 고정화 혼합균에 의한 N 제거 특성을 알아보았다. AUB 최적 배지에서의 반응결과를 보면, 최대 가스 생성율은 PSB:AUB beads의 비율이 10:40 일 때, 0.87ml/h로 가장 높았고, 전체 beads 중 AUB beads의 혼합비율이 높을수록 가스 생성율이 높아짐을 알 수 있었다. PSB:AUB beads의 혼합비율이 50:0, 40:10, 25:25 및 10:40 일 때 pH값은 6.29, 6.01, 5.69 및 5.13으로 나타났다. 이에 반해 미량의 $NH{_4}^+$를 포함하는 PSB 최적배지에서의 반응결과를 보면, 전체 beads 중 AUB가 차지하는 비율이 높을수록 가스 생성양 및 가스 생성율은 현저하게 낮게 나타났다. 이때, pH값은 두 균주의 혼합 비율에 관계없이 약 6.5로 일정하게 나타났다. PSB cubes와 AUB beads를 여러 비율로 섞어 실험한 결과는 PSB beads와 AUB beads를 섞어 반응시킨 결과와 같은 경향을 보였으나 가스의 생성량 및 생성속도가 현저하게 감소하였고, control 반응에서도 가스 생성량이 0.2ml로 매우 적은 양이 발생하였다. 이상의 결과로 보아, $NH{_4}^+$, $NO{_2}^-$ 및 $NO{_3}^-$ 성분이 들어있는 실제 양식폐수의 수질정화에 있어서 PSB:AUB beads를 10:40으로 혼합한 고정화 균주를 이용할 경우 폐수내 N 성분의 동시 제거에 효과적일 것으로 보인다. For efficient removal of nitrogenous compounds produced in recirculating aquaculture system, the N removal characteristics of immobilized mixed microorganisms were investigated at various mixing ratios of photosynthetic bacteria (PSB) immobilized in PVA beads or CTA cubes and ammonium utilizing bacteria (AUB) immobilized in PVA beads. On the optimal medium of AUB, the maxium gas production rate was obtained at the mixing ratio of 10:40 (PSB:AUB), and the gas production rate increased as the portion of AUB beads in the mixed beads increased. When the mixing ratios of PSB:AUB beads were 50:0, 40:10, 25:25 and 10:40, the final pHs were measured to be 6.29, 6.01, 5.69 and 5.13, respectively. On the optimal medium of PSB, however, the volume and the rate of gas production decreased remarkably as the portion of AUB beads in the mixed beads increased. The final pH was measured to be approximately 6.5, regardless of the mixing ratio. In the reactions by the mixed culture of PSB cubes and AUB beads, all results showed the same tendency of those by the mixed culture of PSB and AUB beads, but the volume and the rate of gas production decreased remarkably, even with 0.2ml of gas production in control. From all the results, the use of mixed PSB and AUB beads at the ratio of 10:40 seems to be efficient to remove nitrogenous compounds in wastewater from recirculating aquaculture system.

골관절염 환자를 위한 타이치, 타이치-자조관리 및 자조관리과정의 효과 비교

조경숙,강현숙,김종임,박원숙,송라윤,안양희,임난영,이인옥,최선하,한상숙,Cho, Kyung-Sook,Kang, Hyun-Sook,Kim, Jong-Im,Bak, Won-Sook,Song, Rha-Yun,Ahn, Yang-Heui,Lim, Nan-Young,Lee, In-Ok,Choi, Sun-Ha,Han, Sang-Sook 대한근관절건강학회 2007 근관절건강학회지 Vol.14 No.1

Purpose: The purpose of the study was to compare the effects of Tai Chi exercise, Tai Chi self help program, and self help management program on arthritic pain, stiffness, fatigue and difficulties in performing daily activities in patients with osteoarthritis. Method: The pre-post experimental design was utilized with convenient sampling at Public Health Centers. Total of 38 subjects agreed to participate in the study with the inclusion criteria of being diagnosed as osteoarthritis and no previous regular exercise. The 12 subjects were assigned to Tai Chi exercise group, 13 subjects to Tai Chi exercise combined with self help management program, and the other 13 to self help management program. Tai Chi exercise group performed exercise twice a week for one hour per each session. Tai Chi self help management group came to the class twice a week, one hour for Tai Chi exercise, and another hour for self help management program. The third group came to the class once a week for one hour to participate self help management program. At the completion of 6 week programs, 10 subjects from Tai Chi exercise, 9 from Tai Chi self help, another 10 from self help management groups completed the post-test measures. The data were analyzed by SPSSWIN 11.0. The homogeneity tests for three experimental groups were conducted by $X^2-test$, Fisher's exact test, Kruskal-Wallis test. The final analysis of the post-test measures was conducted by ANCOVA with fatigue, pain, stiffness, and difficulties in daily activities of the pretest measures as covariates. Results: The results were as follows. There were no significant differences in fatigue, pain, and difficulties of daily activities among three groups for the pretest measures. The score of arthritic stiffness was significantly different among groups after controlled for covariates, but the post-hoc tests showed no group differences. Conclusion: The nursing interventions applied for osteoarthritis patients have been previously tested to show the cost effectiveness of symptoms management of this population.

부식된 하수관에서 분리한 곰팡이 OMSOf1 의 황화수소 제거 특성

조경숙,박상진 ( Kyung Suk Cho,Sang Jin Park ) 한국환경생물학회 1997 환경생물 : 환경생물학회지 Vol.15 No.2

A sulfide-oxidizing fungus was isolated from corroded concrete sewer pipes. From the physiological and morphological properties such as growth characteristics on diverse media, reproduction pattern, and size and shape of spores, the isolated fungus was considered as Sporormia sp. The fungus OMSOf1 could oxidize hydrogen sulfide (H_2S) to thiosulfate due to intracellular enzyme systems. The fungus was immobilized in polyurethane carriers which contained super absorbent polymer (sodium polyacrylic acid) permeated with mineral medium, and polyurethane. When 28ppm of H_2S was supplied to the biofilter packed with the carriers at 50㎖/min, more than 95% removal ratio of H_2S was obtained for 15 days without supply of water. The reduction of H_2S removal ratio by drying of the carriers could be recovered by supply of water.

음질을 고려한 환경소음 평가 인자의 기여도분석에 관한 연구

조경숙,조연,황대선,허덕재,Jo Kyoung-Sook,Cho Yeon,Hwang Dae-Sun,Hur Deog-Jae 한국음향학회 2006 韓國音響學會誌 Vol.25 No.3

소음에 대한 인간의 심리적 인지는 소음의 여러 가지 물리적 요인에 의하여 영향을 받는다. 그러나 현재의 환경소음 평가는 A 가중치 등가 소음 수준을 기준으로 평가되고 있으며 인간의 다양한 감성적 요인을 반영하지 못하고 있다. 따라서 본 연구에서는 환경소음 평가를 위하여 환경소음원 43개에 대하여 주관평가와 통계적 분석방법을 사용하여 인간의 감성에 영향을 주는 음질인자 (Sound quality factor)를 분석하였다. 분석방법은 먼저 다양한 환경소음원에 대한 음질물리량 (Sound quality metrics)을 분석하여 음원을 분류하였으며 분류된 음원의 주된 음질 물리량을 고찰하였다. 그리고 쌍대비교법 (Paired comparisons method)과 어의분별척도법 (Semantic differential method)을 사용하여 주관 평가를 실시하고 음질의 요인을 분석하였으며 물리량과의 관계를 규명하였다. 마지막으로 요인에 대한 물리량의 기여도를 분석함으로써 환경소음의 감성적 인지에 대한 기초 물리량을 설정하였다. For the environmental noise assessments. A weighted equivalent noise level (LeqA) is used to measure the time varying environmental noise. However, it is not appropriately reflect various environmental noise features and human emotions. The human perception of the noise is affected largely by the psychoacoustic characteristics of noise as well as the sound pressure level In this study, the effective factors of noise qualify are analyzed using the subjective assessment and statistical analysis of environmental noise, such as road traffic noise. construction site noise, noise in daily living. and other. The analysis methodology is composed to three steps as follows : firstly, the values of the sound qualify metrics of various noise sources were analyzed. And to classify the noise sources, we conducted a cluster analysis using sound quality metrics. Secondly, subjective jury testing was carried out using the methods of paired comparisons and semantic differential. Finally, the correlation between the subjective parameters and the noise quality metrics were analyzed. As a result. the human perception characteristics of the various environmental noise are described in some physical parameters of the noise qualify metrics.

한방의료기관 환자진료부에 의한 암환자 현황에 대한 연구

조경숙,신현규,Cho, Kyung-Sook,Shin, Hyeun-Kyoo 대한암한의학회 2003 大韓癌韓醫學會誌 Vol.9 No.1

Objectives : The former study of Oriental Medicine on cancer has been mostly focused on lab studies with herbal medicine. Among this atmosphere, the aim of this study is to investigate the suitability of Oriental Medicine institutions to participate in cancer registry programme in R.O.K by investigating the type of treatments used for cancer patients in Oriental Medicine institutes. Methods : To investigate the actual conditions of cancer patients who visited Oriental Medicine institutes, a survey was done based on clinical charts of 258 patients of cancer. Results : The order for the percentage of the original tumor organ of the patients, the number of patients who recognized the outbreak on the year of the first visit was the biggest, and the number gradually decreased while the years passed. For the type of treatment, herbal medicine was the most for 92.6%, acupuncture 52.6%, moxibustion 16.7%, cupping 14.7%. It can be seen that multiple treatment was preferred to single treatment. Among the periods for herbal medication, 10 days was the most for 34.1%, 29.8% for within 50 days and 10.5% for within 100 days. The percentage of patients who took treatment of western medicine at the same time and those who took treatment of Oriental medicine only was 47.4% and 43.8% respectively. By surveying categories such as the symptoms, diagnosis of syndrome, principle of treatment from the chart by a free description, there was 841 names of symptoms, 207 diagnoses of syndromes and 206 principles of treatment. But no relation or connection between these could be found statistically. At there were even occasions which the doctor didn't record the cancer itself, precise investigatio for the actual condition of cancer patients in Oriental Medicine institutions appeared to be very difficult. Conclusions : It seems impossible to enroll cancer patients of Oriental Medicine institutions to the cancer registry programme in R.O.K as far as the patients are recorded only under the paradigm of Oriental Medicine. However, if the Oriental Medicine doctors keep a consistency in classifying categories such as cause of death, syndrome diagnosis, principle of treatment and prescriptions, and limit the choices, for each category, a pilot study for cancer registry programme in R.O.K. in Oriental Medicine could be carried out.

일개 국립대학병원의 진료.교육.연구 기능에 대한 평가

조경숙,이해종,이규식,김춘배,설동진,추상채,Cho, Kyung-Sook,Lee, Hae-Jong,Lee, Kyu-Sik,Kim, Chun-Bae,Seol, Dong-Jin,Choo, Sang-Chae 한국병원경영학회 2001 병원경영학회지 Vol.25 No.3

The purpose of this study was to evaluate the environments of patient care, education, and research as indicators of university hospital performance. The objective data for this study were collected by secondary data. The subjective data were collected by a total of 523 questionnaire survey interviews conducted in C university hospital, of which 123 were students, interns, or residents, 76 were professors, and the remaining 324 were patients. The major statistical methods used for the analysis were t-test and ANOVA. We found that the environment of patient care is better than the environment of education and research in C university hospital. While the concern for patient care of C university hospital was about 60%, the equivalent figures for education and research were both 20%. And the patients' satisfaction level was higher than the others. The environment of education and research was found to be deficient in some aspects, especially in such areas as facilities, equipment, and investment. In particular for professors the time for research was very limited because of the demands of patient care. These findings clearly demonstrate that university hospitals need to pay greater attention to the areas of education and research, as well as to patient care. This article concludes with a discussion and summary and presents issues in need of further study.