자세제어와 운동의 협응

정현채(Hyun-Chae Jung) 한국코칭능력개발원 2005 코칭능력개발지 Vol.7 No.1

Helicobacter pylori의 병독성 유전자 및 운동성이 인체 위점막 염증 반응의 병태 생리에 미치는 영향 (1) - 병독 인자 보유 유무에 따른 친염증성 cytokine의 발현능

정현채(Hyun Chae Jung),김정목(Jung Mogg Kim),진영주(Yeoung Joo Jin),이경미(Kyung Mee Lee),김우호(Woo Ho Kim),송인성(In Sung Song),김정룡(Chung Yong Kim) 대한소화기학회 1997 대한소화기학회지 Vol.29 No.5

N/A Background/Aims: CagA or cytotoxin-positive H. pylori may be associated with gastroduodenal diseases. However, controversies about this association also exist. Moreover, there could be geographic differences in the prevalence of virulence factors such as cagA or cytotoxin. It is also not clear whether there are substantial differences among clinical isolates which differ in bearing cagA or cytotoxin in the ability to induce IL-8 gene expression and protein secretion from the gastric epithelial cells. We performed this study 1) to determine the prevalence rate of the genes of virulence factor such as cagA and cytotoxin in H. pylori, 2) to test the ability of cagA cytotoxin', cagA cytotoxin or cagA cytotoxin H. pylori strain to evoke the proinflammatory cytokine expression in cultured gastric epithelial cells respectively. Methods: 1) The prevalence of the cagA gene in H. pylori strains isolated from Koreans was determined by PCR analysis. 2) Cytotoxin assay was performed by the concentrated broth supematants and HeLa cells(ATCC CCL2). 3) Proinflammatory cytokine gene expression was observed by RT-PCR analysis using the RNA extracted from human gastric epithelial cells such as Hs746T(ATCC HTB 135) and AGS(ATCC CRL 1739) infected with H. pylori. Cytokine proteins were also measured by ELISA. Results: 1) 96.8% of H. pylori isolates from Korean adults possessed cagA gene. And 80.6% of H. pylori strains have expressed vacuolating cytotoxicity against HeLa cells within 24 hours. 2) All cagAcytotoxin', cagAcytotoxin or cagA cytotoxin H. pylori strains could evoke the expression of cytokines such as IL-la/p, IL-8, MCP-1 and GM-CSF in the gastric epithelial cells. In case of IL-8, number of molecules of the expressed cytokine transcripts was parallel to the amounts of protein secreted from the gastric epithelial cells infected with H. pylori. Conclusions: These results suggest that H. pylori itself could give rise to express the proinflammatory cytokines in gastric epithelial cells regardless of possessing the known viru1ence factors of H. pylori. (Korean J Gastroenterol 1997;29:579-590)

H . pylori 감염에 의하여 유발되는 인체 위상피세포주에서의 Chemokine 유전자의 발현 - 합성 RNA를 이용한 Interleukin-8과 Monocyte Chemotactic Protein-1 mRNA의 정량

정현채(Hyun Chae Jung),김정목(Jung Mogg Kim),김경아(Kyung A Kim),송인성(In Sung Song),김정룡(Chung Yong Kim) 대한소화기학회 1996 대한소화기학회지 Vol.28 No.2

N/A Background/Aims: Despite the prevalent knowledge that Helicobacter pylori is non-invasive, mucosal infiltration of inflammatory cells has been observed in the gastric mucosa. Chemokines have been implicated in a wide range of acute and chronic int1ammatory processes. We tested whether H, pylori-induced inflammation could be triggered by an array of chernokine genes expressed in human gastric epithelial cells. Methods: After the gastric epithelial cel]s, SNU-5, KATO III and Hs746T, and the non-gastric epithelial cells, HCT-8 and HT-29, were infected ivith H. pylori, cellu]ar RNA was extracted by the guanidinium thiocyanate-phenol-chlorofonm method. Expression of severol chemokine genes was assessed using quantitative reverse transcriptional polymerase chain reaction(RT-PCR). Chemokine mRNA was quantified by RT-PCR using specific primers and synthetic standard RNA generated from pHCQ. Results: Interleukin-8(IL-8) mRNA was expressed in gastric epithelial cells throughout the entire infection period. Monocyte chemotactic protein-l (MCP-l) mRNA was expressed in SNU-5 cells after 9 hours of infection. RANTES(regulated on activation, normal T expressed and secreted) wos not expressed in either cell line. In SNU-5 cells, IL-8 mRNA was hiqhly expressed(1.3 >'10 raolecules/(lg total RNA) after 1 hour and reached its peak ]evel(1.0 10 molecules) at 9 hours. In KATO III ce]ls, IL-8 mRAA expression reached the peak level at 4 hours. Gene expression paralleled the amount of IL-8 protein measured by ELISA(enzyme linked immunosorbent assay). IL-S mRNA expression was not observed in KATO III ceIls infected with C. fetus subsp. Fetus, C. jejuni. And nonpatho- genic E. coli DH5a. IL-8 mRNA expression was increased not only in gastric epithelial cells but also in non-gastric cells infected with H. pylori. Conclusions: These results suggest that an inflammatory reaction induced by H. pylori be initially triggered by chernokines expressed in infected gastric epithelial cells. In addition, H. pylori may have a potential to induce inflammatory reactions in non-gastric epithelial cells. (Korean J Gastroenterol 1996; 28:198-210)

제48차 대한내과학회 추계학술대회초록집 : 심포지움 ; Helicobacter pylori의 최신지견 : 헬리코박터 파일로리 감염의 병태생리

정현채 ( Hyun Chae Jung ) 대한내과학회 1996 대한내과학회지 Vol.51 No.-

전구해양 모의에서 나타난 생지화학과정의 영향

정현채 ( Hyun-chae Jung ),문병권 ( Byung-kwon Moon ),위지은 ( Jieun Wie ),박효진 ( Hyo-jin Park ) 전북대학교 과학교육연구소 2017 과학과 과학교육 논문지 Vol.42 No.2

생지화학 과정이 해양의 평균 상태와 경년 변동에 미치는 영향에 대해서는 여전히 논란중인데 분석에 사용되는 모델이 일치하지 않는 결과를 모의하고 있는 것이 주된 이유다. 우리는 전구 해양 모델을 이용하여 생지화학 과정이 미치는 영향을 조사하였다. 생지화학 과정에 의해서 열대 해양의 연변화는 강화되었고, 전지구 표층 순환의 세기가 세졌다. 특히 서안 경계류와 남극 순환류의 강화가 두드러졌다. 이에 덧붙여, 엘니뇨가 강화된 특징을 보였는데, 우리는 그 원인으로 연직 경압 모드의 변화를 새롭게 제시하였다. 세 개 경압 모드가 포함된 간단한 모형을 이용한 실험 결과로 생지화학 과정에 의해 연직 열적 구조가 바뀌고 결국 경압 모드의 변화로 인해 엘니뇨가 강화된다는 것을 알 수 있었다. 이것은 해양 생지화학 과정의 영향에 관한 새로운 메커니즘으로서 향후 정교한 실험이나 분석에 의해 검증이 필요할 것으로 판단된다. Debate continues about the impacts of biogeochemical processes on the oceanic mean state and interannual variability, since climate models show diverse simulation results. We investigate the biophysical impacts on the ocean climate using an global ocean general circulation model. The annual cycle in the tropical Pacific has been amplified due to biological processes. Global surface currents, particularly western boundary currents and Antarctic Circumpolar current, also have been strengthened. Furthermore, ocean model with biological module shows the larger amplitude of El Niño, which is resulted from the modulation of vertical baroclinic mode. Simple model experiments with three baroclinic modes indicate that the biological-induced changes in oceanic thermal structure act to amplify El Niño amplitude via altered vertical modes. The processes presented in this paper are preliminary and need to be validated using more comprehensive models.

의료인에 대한 죽음 교육으로서 영화의 활용

정현채 ( Hyun Chae Jung ) 대한소화기학회 2012 대한소화기학회지 Vol.60 No.3

Death and dying is an ultimate process that every human being must experience. However, in these days we do not like to think or discuss about death and dying. Actually, hatred and denial is the usual feeling when we encounter death and dying. Dying is more than a biological occurrence. It is a human, social, and spiritual event, but the spiritual dimension of patients is too often neglected. Whether death is viewed as a wall or as a door can have significantly important consequences for how we live our lives. Near death experience is one of the excellent evidences to prove that there should be spiritual component being separated from the human physical body when we experience death. People have called it soul, spirit, or nonlocal consciousness. Caregivers need to recognize and acknowledge the spiritual component of patient care. Learning about death and dying helps us encounter death in ways that are meaningful for our own lives. Among the several learning tools, utilizing cinema with its audio and visual components can be one of the most powerful learning tools in death education. Korean J Gastroenterol 2012;60:140-148)

지구과학 강의에 활용 가능한 그래픽 툴 GMT 소개

정현채 ( Hyun-chae Jung ),위지은 ( Jieun Wie ),이효미 ( Hyomee Lee ),문병권 ( Byung-kwon Moon ) 전북대학교 과학교육연구소 2018 과학과 과학교육 논문지 Vol.43 No.1

Generic Mapping Tool (GMT)은 하와이 대학교에서 지구과학 관측 자료에 대한 가시화를 목적으로 개발된 프로그램이다. GMT는 개인이 소스코드를 다운받아 자유롭게 사용이 가능하도록 제공되고 있다. 이 논문에 설명된 내용은 GMT를 사용하는데 필요한 기초적인 내용을 다루고 있다. 즉, 지구과학 강의나 연구에 활용할 수 있도록, GMT의 기본적인 옵션과 예시를 중심으로 설명하였다. 교사, 학생 혹은 연구자들이 각자의 목적에 맞도록 GMT를 활용하는데 도움이 되기를 바란다. The Generic Mapping Tool (GMT) is developed to visualize Earth science observations at the Hawaii University. GMT is provided for individuals to download the source code freely. In this paper, the basic functionality of GMT is described by focusing on the options and providing examples of GMT for use in Earth science lectures and research. We hope that teachers, students, or researchers will be able to use GMT to achieve their goals.

염증성 장질환의 실험 동물모델

정현채(Hyun Chae Jung) 대한소화기학회 2001 대한소화기학회지 Vol.37 No.2

N/A

Clostridium difficile Toxin A 에 의한 장상피세포에서의 CXC 및 CC Chemokine 의 발현

정현채(Hyun Chae Jung),김정목(Jung Mogg Kim),김주성(Joo Sung Kim),송인성(In Sung Song),김정룡(Chung Yong Kim) 대한소화기학회 2001 대한소화기학회지 Vol.37 No.5

Background/Aims: Intestinal epithelial cells can play a role in signaling the influx of inflammatory cells. We investigated the regulated expression of CXC and CC chemokines by intestinal epithelial cells in response to Clostridium difficile (C. difficile) infection. Methods: Quantitative RT-PCR and ELISA were used to assess the expression of CXC and CC chemokines in human intestinal epithelial cells after stimulation with C. digcile toxin A. To determine the polarity of chemokine secretion, chemokine production was measured from culture supernatants of Caco-2 cells which were cultured in transwell chambers. Results: The expression of the CXC chemokine, GRO-α and IL-8, increased in the first hr after stimulation. In contrast, the expression of epithelial neutrophil activating protein (ENA)-78 mRNA was delayed for 18 hr. The CC chemokine monocyte chemotactic protein (MCP)-1 mRNA, was expressed in 3 hr after stimulation. Upregulated mRNA expression of chemokines was paralleled by the increase of protein levels. After stimulating Caco-2 cells with toxin A, CXC and CC chemokines were released predominantly into the basolateral compartment. Moreover, the addition of IFN-y and tumor necrosis factor (TNF)-a to toxin A-stimulated Caco-2 cells showed an increased basolateral release of CC chemokine, MCP-1. Conclusions: These results suggest that the expression of CXC and CC chemokine in the epithelial cells stimulated with C. difficile toxin A may be an important factor in the mucosal inflammatory response. (Korean J Gastroenterol 2001;37:345-355)

인체 대장 상피세포 및 대장점막에 발현된 여러 Cytokine 유전자의 정량분석을 통한 인체 숙주 방어기전에 관한 연구 - 합성 RNA 를 이용한 정량적 역전사 PCR 법의 응용

정현채(Hyun Chae Jung),김정목(Jung Mogg Kim),송인성(In Sung Song),김정룡(Chung Yong Kim) 대한내과학회 1995 대한내과학회지 Vol.49 No.1

N/A Objectives: We performed a series of quantitative analysis of mRNA using reverse transcription(RT) PCR which is not only more sensitive than Northern blot but also does not require any radioisotope. For this purpose, standard RNA was designed and synthesized as the standard for quantitative RT-PCR. Using this standard RNA, we established the method of cytokine mRNA quantitation. We also tested whether imaging densitometry could replace the isotope use or not while in quantitation. According to this method, first, we studied cytokine gene profiles on human colonic epithelia cells, second, analyzed quantitatively those on the cells infected by the invasive enteric bacteria. Finally, we investigated the IL-8 mRNA expression of colonic mucosa in a patient with ulcerative colitis and a normal subject. Methods: The plasmid pHCQ encoding the synthetic RNA has the 5' and 3' priming sites for IL-1α, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-8, IL-10, TNF α, TGFβl, IFN-γ, β-actin, TCRα, TCRδ, CD4, and CD8, and the pGMC has those for MCAF and GM-CSF. Their arragement was designed to yield PCR products that differ in size by about 100 bp from those of the target RNA. After their sense and antisense primers were linked serially by oligo-nucleotide overlap extension and PCR amplification, each primer cassettes and poly(A) taill were subcloned into the plasmid, pGEM 3Zf(-), in order. And complementary standard RNAs were obtained by in vitro transcription. Using these standard RNA encoded from pHCQ and pGMC, we carried out quantitative RT-PCR for target RNAs extracted from cultured cells or tissue. Target RNAs were extracted by method using guanidinium thiocyanate-phenol-chloroform. Resu1ts: The size differences of PCR products from the standard RNA and the extracted target RNA were matched as designed. And the results obtained from either 32P-labelled PCR primer or imaging densitometry were also same. When the cultured colonic epithelial cells were infected by the invasive bacteria or stimulated by TNFα, proinflammatory cytokines, such as IL-8, TNFα, and IL-1β, were upregulated. And IL-8 mRNA was upregulated at the mucosa in a patient with ulcerative colitis comparing to that in a normal subject, Conclusion: These results suggest that synthetic standard RNAs encoded from pHCQ and pGMC are able to be used in quantitation of cytokine mRNA from cultured cells or biopsied tissue. In addition, human colonic epithelial cells may play a role in the host immune response by expressing mRNA for an array of cytokine which are already known to be important in the inflammatory reaction. In the near future, quantitative RT-PCR will be used widely in the studies of expression of specific human genes.