흡연자와 비흡연자간의 구강 내 세균 분포 및 항균제 감수성

정현자,김수정,Jeong, Hyun-Ja,Kim, Su-Jung 한국미생물학회 2010 미생물학회지 Vol.46 No.4

흡연은 음주와 더불어 치과질환을 유발하는 원인인자로 알려져 있으나, 흡연자와 비흡연자간의 구강 내 분포하는 미생물을 조사한 연구는 현재 매우 미비한 상태이다. 본 연구에서는 흡연자 30명, 비흡연자 30명으로 나누어 구강 내 분포하는 세균을 분리하고 혈액한천배지에서 배양하였다. 분리된 병원성 세균은 Kirby-Bauer 항생제 감수성 검사 방법으로 항생제에 대한 감수성 및 내성을 검사하였다. 각각의 콜로니는 그람 염색을 하였고 자동 동정기인 VITEK을 이용하여 동정하였다. 그람 염색법을 이용한 결과 흡연자의 구강에서는 총 41개 균주가 분리되었고, 그 중 그람 양성 구균 63%, 그람 음성 구균 29%, 그람 양성 간균 3%, 그람 음성 간균 5%가 나타났고, 비흡연자의 구강에서는 총 38개 균주가 분리되었고 그 중 그람 양성 구균 55%, 그람 음성 구균 26%, 그람 양성 간균 3%, 그람 음성 간균 16%가 나타났다. VITEK system을 이용하여 세균의 특이적 분포를 조사한 결과 흡연자의 구강에서는 Streptococcus mutans (6/41), Gemella morillorum (6/41), Streptococcus oralis (2/41), Streptococcus pneumoniae (1/41), Staphylococcus aureus (3/41), Streptococcus anginosus (1/41), Streptococcus intermedius (1/41), Streptococcus uberis (1/41), and Streptococcus sanguinis (1/41) 반면 비흡연자의 구강에서는 Streptococcus sanguinis (8/38), Staphylococcus aureus (1/38), Staphylococcus auricularis (1/38), Streptococcus uberis (1/38), Streptococcus intermedius (1/38), Streptococcus mutans (1/38), and Streptococcus oralis (1/38)로 나타났다. 3명의 흡연자에서 분리된 S. aureus는 Beta-lactamase를 분비하는 MRSA로 동정된 반면 비흡연자 한 명에서 분리된 S. aureus는 Beta-lactamase를 분비하지 않고 methicilin에 감수성을 보였다. 흡연자와 비흡연자의 상재균은 다른 분포를 보였고 특히 G. morillorum와 MRSA가 흡연자의 구강에서 흔히 발견됨을 알 수 있었다. 이러한 결과들은 흡연자의 구강질환을 예방 및 치료에 유용할 것으로 사료된다. It is well known that smoking as well as drinking is a factor of stomatopathy, however there are few investigations about comparison of oral flora between smokers and non-smokers. In this study, we isolated the oral flora of 30 smokers and 30 non-smokers and cultured them on blood agar plates. The isolated pathogenic microorganisms were tested for antibiotic susceptibility and resistance using the Kirby-Bauer antibiotic testing method. Each colony was stained using the Gram staining method and was identified by an automatic identifier, known as the VITEK system. We isolated 41 colonies from smokers' oral cavity, and they were sorted as 63% of Gram-positive cocci, 29% of Gram-negative cocci, 3% of Gram-positive bacilli, and 5% of Gram-negative bacilli by gram staining, whereas 38 colonies were isolated from non-smoters' oral cavity, and their proportions were 55% of Gram-positive cocci, 26% of Gram-negative cocci, 3% of Gram-positive bacilli, and 16% of Gram-negative bacilli. The VITEK system revealed specific distribution of bacteria species that Streptococcus mutans (6/41), Gemella morillorum (6/41), Streptococcus oralis (2/41), Streptococcus pneumoniae (1/41), Staphylococcus aureus (3/41), Streptococcus anginosus (1/41), Streptococcus intermedius (1/41), Streptococcus uberis (1/41), and Streptococcus sanguinis (1/41) in smokers oral cavity whereas Streptococcus sanguinis (8/38), Staphylococcus aureus (1/38), Staphylococcus auricularis (1/38), Streptococcus uberis (1/38), Streptococcus intermedius (1/38), Streptococcus mutans (1/38), and Streptococcus oralis (1/38) in those of non-smokers'. Three cases of Staphylococcus aureus from smokers produced Beta-lactamase and were identified methicillin-resistance Staphylococcus aureus (MRSA). However one case of Staphylococcus aureus from non-smoker did not produce Beta-lactamase and was sensitive to methicillin. In conclusion, the distribution of oral flora was different between smokers' and non-smokers' oral cavity, especially Gemella morillorum and MRSA were predominantly found in smoker's oral cavity. These results are useful in the treatment and prevention of patients with stomatopathy caused by smoking.

Determination of Gross-${\beta}$ and ${\gamma}$-Ray Activity Concentrations of Human Tooth

정현자,강현경,김성환,Jeong, Hyunja,Kang, Hyun-Kyung,Kim, Sunghwan Korean Society of Radiological Science 2014 방사선기술과학 Vol.37 No.4

10세에서 70세까지 국내 남녀를 7개 연령대별 그룹으로 구분한 후, 치아의 감마선 농도와 각 그룹별 전${\beta}$ 방사능 농도를 측정하였다. 전${\beta}$ 농도는 P10가스(아르곤 90%, 메탄 10%)를 충진한 Tennelec XLB 측정기로 측정하였으며, 감마선은 고순도 게르마늄 검출기를 사용하여 감마선분광분석법으로 측정하였다. 본 실험에서 측정된 여성의 전${\beta}$ 방사능 농도범위는 0.089~0.32 Bq/kg이었으며, 남성의 전${\beta}$ 방사능 농도범위는 0.13~0.26 Bq/kg이었다. 치아의 감마선분광분석 결과 자연방사성동위원소인 $^{40}K$, $^{208}Tl$, $^{228}Ac$ 및 $^{234}Th$가 검출되었으며, 측정된 감마선 방사선 농도는 각각 20.7, 21.9 3.88 및 5.24 Bq/kg 이었다. 본 연구 결과는 향후 후쿠시마 원전사고 등 불의의 방사선 누출 사고 등에 대비하여 정상 환경에서의 인체의 치아에 축적된 방사능 준위 데이터로 활용할 수 있을 것으로 사려된다. The ${\gamma}$-ray concentration and gross-${\beta}$ activity by age group were measured in the teeth of males and females of the domestic residents. They were divided into 7 age groups from 10s to the age of 70s. The gross-${\beta}$ activity concentration was measured by using the Tennelec XLB measuring instrument filled with P10 gas (argon 90%, methane 10%). The ${\gamma}$-ray was measured through the ${\gamma}$-ray spectroscopic analytical method by using the high purity germanium (HPGe) radiation detector. The range of gross-${\beta}$ activity concentration was measured 0.089 to 0.32 Bq/kg in females and 0.13 to 0.26 Bq/kg in males. From the ${\gamma}$-ray spectroscopic analysis of the teeth, the natural radioactive isotopes of $^{40}K$, $^{208}Tl$, $^{228}Ac$ and $^{234}Th$ were detected and their measured ${\gamma}$-ray activity concentrations were found to be 20.7, 21.9, 3.88 and 5.24 Bq/kg, respectively.

농촌지역 일부 노인의 구강처치 프로그램수행 및 구강관리의식조사

정현자 ( Hyun Ja Jeong ),정기옥 ( Gi Ok Jung ),김혜진 ( Hye Jin Kim ) 한국위생과학회 2008 한국위생과학회지 Vol.14 No.2

튜터링 프로그램에 참여한 D대학 대학생들의 자기조절 학습능력 수준에 관한 조사

정현자(Jeong, Hyun-Ja),표창우(Pyo, Chang-Woo) 한국산학기술학회 2011 한국산학기술학회논문지 Vol.12 No.1

본 연구의 목적은 튜터링 프로그램에 참여한 D대학 학생들의 일반적 특성과 연령에 따른 자기조절 학습능 력의 차이와 튜터와 튜티의 자기조절 학습능력의 차이를 인지조절과 동기조절 및 행동조절로 분류, 조사하여 효율적 인 튜터링 프로그램 운영에 필요한 기초자료를 확보하기 위한 것이다. 2010년 4월 26일부터 6주간 실시된 튜터링 프로그램에 참여한 15개 학과 대학생 200명을 대상으로 자기조절 학습능 력에 관한 설문지를 배포하여 자기기입식 응답법으로 응답한 183명의 자료를 조사하였다. 연구결과 참여자 중 20세 이상의 대학생들은 자기조절학습능력 중 조직화, 메타인지전략의 사용, 계획, 자아효능감, 학업시간관리 능력이 유의 하게 높았다(p<0.05). 또한 튜터는 튜티에 비하여 인지전략의 사용, 시연, 조직화, 메타인지전략의 사용, 계획, 점검, 숙달목적지향성, 자아효능감, 성취가치, 행동통제와 도움을 구하기 능력이 높았다(p<0.05). 결론적으로 튜터링 프로그 램에 참여한 학생들이 1~2년의 학습과정을 통해 전반적인 자기조절학습능력이 높게 나타났음을 확인할 수 있다. 따라서 전문대학에서는 상대적으로 자기조절 학습능력이 부족한 신입생 및 복학생들을 대상으로 상대적인 자기조절 학습능력이 우수한 튜터를 양성하여 튜터링 프로그램과 같은 양질의 학생지원 프로그램을 지원할 필요가 있으며, 향 후 성적이 우수한 선배가 후배들을 지도할 수 있는 학습 환경을 제공하고, 다양한 프로그램을 연구, 개발한다면 튜터 링 프로그램이 점차 차별화되고 효율적인 자기 조절 학습능력향상을 도모할 수 있을 것으로 사료된다. The purposes of this study are to find out the effectiveness of the tutoring program and the level of self-regulated learning ability in tutoring program attending students. This study has been performed for team-tutoring program attending students(n=183) who are in 15 department, D college in Daegu. Following results were drawn thought correlation analyses of variables obtained during the survey period. The results were as follows; There was statistically significant difference among perception controlling ability, purpose controlling ability, and action controlling ability. In over 20 ages, organization, meta–perception stratagem, arrangement, self-effectiveness, controlling learning time ability were higher than of lower 20 ages(p<0.05). In tutors, demonstration, organization, meta-perception stratagem, arrangement, checking, purpose intentions, self- effectiveness, achievement, controlling action, help requirement ability were higher than tutees(p<0.05). As results, the tutoring program was effective for both tutors and tutees in college students. Further studies in an education program for students in all years should be implemented to examine tutoring effects. Implementation of tutoring should address the frustrations and difficulties encountered by the students to facilitate better outcomes.

당뇨병 이환유무에 따른 구강건강상태에 대한 조사연구

정현자 ( Hyun Ja Jeong ),최옥선 ( Ok Sun Choi ),김진경 ( Jin Kyung Kim ) 한국위생과학회 2005 한국위생과학회지 Vol.11 No.1

한·일 치위생(학)과 현황과 일부 대학의 교육 과정 비교

정현자(Jeong, Hyun-Ja),후지와라(Aiko, Hujiwara) 한국산학기술학회 2010 한국산학기술학회논문지 Vol.11 No.12

2010년 D대학 치위생과 3학년 200명을 대상으로 해외 진학 및 취업에 관심이 있는 아시아 국가를 조사한 결과, 일본이 114명(60.3%)으로 가장 높게 나왔고, ‘기회가 된다면 아시아권 해외 진학 및 취업을 희망하십니까?’라는 질문에 그렇다가 72명(38.1%)으로 가장 많았다. 가장 관심이 높았던 일본은 2년제 51개 대학, 3년제 101개 대학, 4년 제 6개 대학으로 총 158개 대학에서 치위생학과가 개설되어 있었고, 학교 법인은 관동지방(간토)이 25개로 가장 많 았다. 일개 학제별 한․일 교육과정을 분석한 결과, 동일하거나 유사한 교과목이 다수였으며, 3년제는 39개 4년제는 44 개였다. 일본의 국가고시 교과목이 19개 과목으로 해부학 외 9개 과목이 100점, 치과임상개요의 치과임상개론 외 8개 과목이 50점, 치과진료보조 외 1과목이 50점으로 총 배점이 200점이였고, 일본의 4년제 치위생사양성과정은 졸업 후 치과위생사 면허와 함께 동경의과치과대학 치과구강보건학과에서 사회복지사, 히로시마대학 치과 구강건강과학과는 양호교사(보건교사) 1종 면허 등을 선택적으로 동시에 자격을 취득할 수 있도록 업무영역을 확대한 교육과정을 편성 하고 있다. This study was conducted in order to promote students’ interest in overseas employment and to present methods of improving education that suits globalization and internalization. From responses to individual written survey questionnaires answered by 3 rd grade 200 students who are studying dental hygiene at colleges in Daegu. They had most interested in Japan (60.3%), and they want to work abroad if they are given the changes (38.1%), which indicate that the dental hygiene majors have much interest and positive attitude in overseas employment. In Japan, there are 158 schools had department of dental hygiene (51 to 2 years, 101 to 3 years, 6 to 4 years). Especially, Kanto area has 25 private schools. When compared Korea and Japan, they similar were curriculum, 39 at 3 years, 44 at 4 years, respectively. The current national board dental hygiene examination system was 200 in total score, and 19 in total subjects. The 9 basic clinical courses including oral anatomy have 100 scores, and the 8 subjects including preclinical course have 50 scores, one dental assistant course have 50 scores. In Japan, 4 years pre-dental hygiene curriculum content provide certificate of dental hygiene after graduation. They provide additionally ‘social worker’ in Tokyo University, ‘teacher in nursing’ in Hirosima University. They scheduled the various foundational knowledge and skill such as competencies to communicate effectively, to participate community health program, and to make decisions regarding dental hygiene service.

대용량 플라스틱 섬광체를 이용한 치과 X-ray 촬영실의 공간선량 측정

정현자(Jeong Hyun-Ja) 한국콘텐츠학회 2022 한국콘텐츠학회 종합학술대회 논문집 Vol.2022 No.7

일부 성인의 구강보건지식과 행동변화에 따른 치면 내 구강미생물 변화

정현자(Hyun-Ja Jeong),김혜진(Hye-Jin Kim) 한국구강보건과학회 2018 한국구강보건과학회지 Vol.6 No.2

This study was conducted to present biological grounds as quantitative criteria by collecting causative organisms of periodontal diseases. A comparative analysis was conducted after implementing periodontal management based on a dental hygiene process. A further goal was to understand the role of oral health professionals for preventing periodontal diseases. 26 persons who visited the Clinical Dental Hygienist Lab of D. University from September 28, 2017 through November 30, 2017, were final research subjects. This study obtained the following conclusions by analyzing oral microorganisms through real-time PCR by dental examination and oral collection (tooth surface and gingival crevicular fluid). As a result of a comprehensive analy-sis of the results of this study, bacteria in the tooth surface and the gingival sulcus tended to decrease according to oral health knowledge, oral health practice, and verbal oral health literacy by implementation of periodontal management. Thus, it is judged that it is necessary to check the effect of the implementation of periodontal management based on the dental hygiene proc-ess with patients who have periodontal diseases, and at the same time to conduct an evi-dence-based self-directed performance in the process of dental hygiene management in order to solve the subjects’ oral health problems. In addition, it is judged that it is crucial to the needs and importance of ongoing management for patients with periodontal diseases to conduct a broader follow-up study, e.g. Understanding characteristics by which certain bacterial species appear.

칫솔질 방법에 따른 치은염 환자의 구강건강상태지수 변화

정현자 ( Hyun Ja Jeong ),김혜진 ( Hye Jin Kim ),정애화 ( Ae Hwa Jeong ) 한국치위생학회(구 한국치위생교육학회) 2011 한국치위생학회지 Vol.11 No.4

Objectives : This study purpose were the effect of toothbrushing for decreasing halitosis for gingivitis and periodontitis patients. Methods : The university staffs were examined oral condition and analysised a change of oral health state score after using 3 types of toothbrushing. Results : The results were as followed : OHI-S shows that the decreasing effect takes place in the M. bass method and Tooth pick method, but shows no differences by each method. GI for Self test method shows decreasing effect after 2nd week during education while the M. bass method and Tooth pick method shows after 1st week during education. The M. bass method shows much greater effect of halitosis amongst 3 kind of method. PI for Self test method and Tooth pick method show decreasing effect. Decreasing effect during education shows until 2nd week by 3 kind of method, but it shows no differences after 3rd week. VSC(ppb) for M. bass method and Tooth pick method show decreasing effect. Decreasing effect during education shows after 3rd week by 3 kind of method, but it shows no differences until 2nd week. PHP for Self test method, M. bass method and Tooth pick method show decreasing effect after 2nd week during education. But, there is no differences of decreasing effect by among 3 kind of method. Conclusions : This study reports that it is necessary to carry outt further studies on the improvement of oral health management of adults based on the development of oral health education. (J Korean Soc Dent Hygiene 2011; 11(4): 595-602)

구강에서 분리한 Enterococcus faecalis CHJ-5에 대한 생약재의 항균효과

정현자 ( Hyun Ja Jeong ),조명숙 ( Myung Sook Cho ),지원대 ( Won Dae Ji ) 한국치위생학회 2001 한국치위생학회지 Vol.1 No.1