Antitumor and Immunological Effects of Bovine Thymic Extract

정용자,홍기태,정현옥,Chung, Yong Za,Hong, Gi Tae,Jung, Hyun Ok Korean College Of Clinical Pharmacy 1992 한국임상약학회지 Vol.2 No.1

Thymic extract showed antitumor effect to sarcoma mice with higher dose$(200{\mu}g/mouse/day$ i.p., 4weeks) but not with low dose$(5{\mu}g/mouse/day$ i.p., 6 weeks). Direct cytotoxicities were exhibited against sarcoma 180, L1210 and MOLT-4 by MTT assay. The spleen weight of mice were increased but the number of circulating lymphocytes were not increased after long-term(2 weeks) administration of thymic extract. Evaluating the mitogenesis by MTT assay. $\%$ absorbance of human lymphocytes was not increased by thymic extract. Cell cycle statistics of S phase and $G_2/M$ phase was not increased in the presence of that by PI staining. The formation of rosette was induced, irrespectively of exposure time short-term(l hour) and long-term(2 weeks). The population of mouse blood T-cell to bind Lyt2-antimonoclonal antibody and to $L_2T_4$ were increased after administration of thymic extract$(2-200{\mu}g/mouse/day)$. From the above results, it is suggested that thymic extract exerts antitumor activity by stimulating T cells to differeniate in vivo but not in vitro.

중금속의 독성 기전에 관한 연구 (Ⅰ)

정명규(Myung Kiu Chung),임경택(Gyong Taek Lim),정현옥(Hyun Ok Jung) 大韓環境工學會 1994 대한환경공학회지 Vol.16 No.5

Effects of Barbiturates on the Fluidity of Phosphatidylethanolamine Model Membranes

윤일,김형일,황태호,김종렬,김인세,정용자,신영희,정현옥,강정숙,Yun, Il,Kim, Hyung-Il,Hwang, Tae-Ho,Kim, Jong-Ryol,Kim, In-Se,Chung, Yong-Za,Shin, Yong-Hee,Jung, Hyun-Ok,Kang, Jung-Sook The Korean Society of Pharmacology 1990 대한약리학잡지 Vol.26 No.2

Barbiturates가 분자적 약리작용기전 탐구에 기초자료를 제공하기 위하여 소의 신선한 대뇌피질 synaptosomal plasma membrane vesicles로부터 분리제제한 phosphatidylethanolamine인공세포막(SPMVPE)의 유동성에 미치는 barbiturates의 영향을 형광 probe법으로 검색한 결과는 다음과 같다. 1. Pentobarbital, hexobarbital, amobarbital 및 phenobarbital이 기재한 순위로 SPMVPE내 Py-3-Py의 monomer 형광세기에 대한 excimer 형광세기의 비 (I'/I)를 감소시켰다. 2. Pentobarbital, hexobarbital, amobarbital 및 phenobarbital이 기재한 순위로 SPMVPE내 DPH의 polarization, anisotropy, limiting anisotropy, order parameter 및 rotational relaxation time을 증가시켰다. 3. 따라서 위에 제시한 barbiturates가 SPMVPE의 유동성을 유의성있게 감소시킨다는 것을 확인할 수가 있었다. Intramolecular excimer formation with 1,3-di(1-pyrenyl)propane (Py-3-Py) and fluorescence polarization with 1,6-diphenyl-1,3,5-hexatriene (DPH) were used to evaluate the effects of barbiturates on the bulk fluidity of the model membranes of phosphatidylethanolamine fraction of synaptosomal plasma membrane vesicles (SPMVPE) isolated from bovine cerebral cortex. In the SPMVPE, barbiturates decreased the excimer to monomer fluorescence intensity ratio (I'/I) of Py-3-Py and increased the fluorescence polarization (P), anisotropy (r), limiting anisotropy $(r_{8})$, order parameter (S) and rotational relaxation time $({\bar{P}})$ of DPH in a dose-dependent manner. The relative potencies of barbiturates to order the SPMVPE were in the order: pentobarbital > hexobarbital > amobarbital > phenobarbital. Hence, it is concluded that barbiturates have ordering effects on the SPMVPE. And the membrane-ordering potencies of barbiturates appear to be correlated with the potencies for enhancement of GABA-stimulated chloride influx and with the anesthetic effects of barbiturates.

Barbiturates가 소의 신선한 대뇌피질 Synaptosomal Plasma Membrane Vesicles로 부터 추출하여 제제한 Phosphatidylethanolamine 인공세포막의 유동성에 미치는 영향

윤일(Il Yun),김형일(Hyung-Il Kim),황태호(Tae-Ho Hwang),김종렬(Jong-Ryoul Kim),김인세(In-Se Kim),정용자(Yong-Za Chung),신영희(Yong-Hee Shin),정현옥(Hyun-Ok Jung),강정숙(Jung-Sook Kang) 대한약리학회 1990 대한약리학잡지 Vol.26 No.2

Barbiturates가 분자적 약리작용기전 탐구에 기초자료를 제공하기 위하여 소의 신선한 대뇌피질 synaptosomal plasma membrane vesicles로부터 분리제제한 phosphatidylethanolamine인공세포막(SPMVPE)의 유동성에 미치는 barbiturates의 영향을 형광 probe법으로 검색한 결과는 다음과 같다. 1. Pentobarbital, hexobarbital, amobarbital 및 phenobarbital이 기재한 순위로 SPMVPE내 Py-3-Py의 monomer 형광세기에 대한 excimer 형광세기의 비 (I /I)를 감소시켰다. 2. Pentobarbital, hexobarbital, amobarbital 및 phenobarbital이 기재한 순위로 SPMVPE내 DPH의 polarization, anisotropy, limiting anisotropy, order parameter 및 rotational relaxation time을 증가시켰다. 3. 따라서 위에 제시한 barbiturates가 SPMVPE의 유동성을 유의성있게 감소시킨다는 것을 확인할 수가 있었다. Intramolecular excimer formation with 1,3-di(1-pyrenyl)propane (Py-3-Py) and fluorescence polarization with 1,6-diphenyl-1,3,5-hexatriene (DPH) were used to evaluate the effects of barbiturates on the bulk fluidity of the model membranes of phosphatidylethanolamine fraction of synaptosomal plasma membrane vesicles (SPMVPE) isolated from bovine cerebral cortex. In the SPMVPE, barbiturates decreased the excimer to monomer fluorescence intensity ratio (I /I) of Py-3-Py and increased the fluorescence polarization (P), anisotropy (r), limiting anisotropy (r₈), order parameter (S) and rotational relaxation time (P ̅) of DPH in a dose-dependent manner. The relative potencies of barbiturates to order the SPMVPE were in the order: pentobarbital > hexobarbital > amobarbital > phenobarbital. Hence, it is concluded that barbiturates have ordering effects on the SPMVPE. And the membrane-ordering potencies of barbiturates appear to be correlated with the potencies for enhancement of GABA-stimulated chloride influx and with the anesthetic effects of barbiturates.

Cytotoxicities of Dimethylsulfoxide and Glycerol on Proliferative and Quiescent BALB/c 3T3 Fibroblasts estimated by MTT colorimetric assay

Chung,Young-Za,Jung,Hyun-Ok,Lee,Byung-Hwang 慶星大學校 1992 論文集 Vol.13 No.3

Dimethylsulfoxide(DMSO)와 glycerol은 비수용성 물질의 용매나 냉동 보호제로 널리 사용되고 있다. DMSO 또는 glycerol를 사용하였을 때 세포에 미치는 독성을 검색하기 위하여 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide(MTT) 비색 분석법을 이용하였다. 증식 및 정지 상태의 BALB/c 3T3 섬유아세포는 세포 수의 증가에 따라 540nm에서 홉광도가 증가함으로, DMSO 혹은 glycerol의 처치후 홉광도의 감소는 세포 독성의 증가로 간주되었다. 증식중인 세포가 정지 상태의 세포보다 일반적으로 DMSO와 glycerol에 의한 손상이 더 컸었다. 증식중일 때는 DMSO 및 glycerol의 농도가 증가할수록 세포 독성도 증가하였으나 정지 상태일 때는 DMSO와 glycerol은 세포 손상을 나타내기는 하나 농도 증가에 따른 독성의 증가가 뚜렷하지는 아니하였다. 증식중인 세포에 대하여 만성폭로시는 급성 폭로시보다 세포 독작용이 더 우세함을 보여 주었다. 그리고 가장 낮은 농도(0.2%v/v)에서 조차 만성 폭로시 뿐아니라 급성 폭로시에도 세포독성이 있음을 보여 주었다. 한편, 정지 상태일 띠는 세포 독성이 폭로시간의 경과에 따라 증가하긴 하지만 낮은 농도에서는 세포독성이 미약하였으며, 농도 증가에 따른 세포 독성의 뚜렷한 증가도 증식중인 세포에 비하여 훨씬 낮음을 보여 주었다. The cytotoxicities of dimethylsulfoxide (DMSO) and glycerol were evaluated in proliferative and quiescent BALB/c eT3 fibroblasts under normal condition, using MTT colorimetric assay. There was a good correlation between the absorbance by MTT assay and the number of viable cells. By treatment with DMSO or glycerol, the absorbance was decreased dose-dependently, representing the increase of cytotoxicity. This cytotoxicity was much more higher against proliferative cells than against quiescent cells. In the proliferative state, the increase of cytotoxicity induced by DMSO or glycerol was dependent on dose and duration of incubation time in acute(10 minutes after treatment) and chronic(48 hours after treament) exposure. However, the cell damage by DMSO or glycerol were not noticeable in the quiescent state but increased with administered dose. In summary, the cytotoxicity by DMSO or glycerol against proliferative BALB/c 3T3 fibroblasts was increased, depending on dose and exposure time, and much higher than that against the quiescent cells. Even the lowest dose(0.2%) of DMSO or glycerol may damage the growth of the fibroblasts.

Purification and Characterization of Lectin from Epimedium Koreanum Nakai

Park,Jung-Ok,Suh,Soo-Suh,Jung,Hyun-Ok,Chung,Yong-Za,Hwang,Jin-Young 慶星大學校 1992 論文集 Vol.13 No.2

Epimedium Koreanum N????로부터 lectin을 이온 교환 chromatography와 gel filtration후 전기영동하여 분리 정제하였다. 이 lectin은 분자량이 47 kd인 단량체이었으며, 58.2%의 단밸질과 41.8%의 탄수화물로 이루어져 있었고, xylose, aspartic acid 및 phenylalanine의 구성비가 높았다. 혈구응집 반응도 여러 동물의 적혈구에서, 그리고 사람의 혈액에 대하여도 혈액형에 관계없이 응집을 일으켰다. 혈구 응집 반응은 생쥐의 적혈구를 사용하여 실험하였을 때, 25 mM fructose 및 50 mM arabinose, N-acety-D-glucosamine, N-acetyl-D-galactosamine에 억제되었으며, 나아가 5 mM Ca++, Ba++, Mg++의 존재하에서 억제되었다. 한편 propidium iodide 염색 후 세포주기 분석을 하여 mitogenic activity를 검색하였을 때 이 lectin은 사람이나 생쥐의 림프구를 증식시키지는 못하였다. Lectin from Epimedium Korean urn N???? was purified by ion exchange chromatography and gel filtration. This lectin was a monomer of M. W. of 47 kd and a glycoprotein composed of 58.2% carbohydrates and 41.8% protein each on a weight basis. Among those, xylose, aspartic acid and phenyl-alanine residues was included with relatively high percentages. This lectin was shown to cause hemagglu-tination with erythrocytes of various animals including human erythrocytes irrespective of blood types, and thus considered as Epimedium Koreanum Agglutinin(EKA). This hemagglutination was inhibited in the presence of 25 mM fructose and 50 mM of each arabinose, N-acetyl-D-glucosamine, and N-acetyl-D-galactosamine. Furthermore, metal ions such as Ca++, Ba++ and Mg++ (5 mM each) inhibited hemagglutination It was not seemed that EKA is mitogenic to human lymphocytes when cell cycle analysis was tested with propidium iodide staining by flow cytometer.