미생물의 포도당 이성화효소에 관한 연구 (제2보) Streptomyces sp . K-14 균주의 배양특성에 관하여

정태화(Tai Wha Chung),한문희(Moon H . Han) 한국미생물생명공학회 1976 한국미생물·생명공학회지 Vol.4 No.4

우리나라의 장티브스 발생(發生)에 대(對)한 역학적(疫學的) 고찰(考察)

정태화 ( Tai Wha Chung ),김영의 ( Yeung Eui Kim ),전남호 ( Nam Ho Chun ),신광훈 ( Kwang Hoon Shin ) 대한임상검사과학회 1972 대한임상검사과학회지(KJCLS) Vol.4 No.1

Typhoid fever is one of the most serious health hazards in Korea. Although the incidence rate has been assumed to be very high. This study was carried out using the records of the acute communicable diseases reported to the Ministry of Health and Soclal Affalrs during past 10 years from 1961 to 1970. The results are summarized as follows: 1. Typhoid fever cases were 60.2 percent of the class 1 communicable disease cases in the past 10 years in Korea. 2. The Morbidity rate for typhoid fever .was 14.9 based on 100,000 population, and the case fatality ratc was 2. 1 percent. 3. The high epidemic period is from May to October each year. The frequency of incidence was highest in July. 4. The incidence is higher in the rural areas, lower in urban area, but lowest in mountainous districts. 5. The number of typhold fever mobidity was higher amongmales than females and the high incidence age group was the 10 to 35 age groups (Median 22.35 years, standard deviation 12.50) 6. Most typhoid fever cases were diagnosed by clinical dispensary. (Clinical dispensary diagnosis 71.3 %, serological examination 24.3 %, bacteriological examination 4.4 %) 7. The expenditure on typhiod vaccine averaged 20.8 percent of population based on 0.5 ml doses given to the whole population. 8. Piped mineral water supply ratio was 24.0 percent based on the total number of houses in Korea in 1970. The authors recommend the need for nationwide hea1th education and increased safety of drinking watcr supply system in the rural areas. The cnotrol and detecion of carriers should be processed bacteriologically and serological diagnosis and annually vaccintions should be given to the age group from 9 to 35 years seasonally in March or April.

한강하류수역의 기초생산과 식물플랭크톤 색소량의 년변화

최상,정태화,곽희상,Choe, Sang,Chung, Tai Wha,Kwak, Hi-Sang 한국해양학회 1968 韓國海洋學會誌 Vol.3 No.1

This study was undertaken to assess the annual cycle of primary production and plant pigments in a downstream of the Han River. Measurements were carried out at three week intervals during April 1966 and March 1967, and ancillary data include water temperature, transparency, pH, dissolved oxygen and phytoplankton cell number. The seasonal cycle in water temperature profile shows the hihgest in the end of August with 27$^{\circ}C$, lowest in the middle of February with 0.2$^{\circ}C$. The transparency with Secchi disk reading varied from a maximum 4.0m in fall and a minimum 0.5m or less in early spring and flood season of summer. The pH of the river water varied from 6.5 to 7.3, averaged 6.91 in the surface water and 6.98 in the bottom water, showed little seasonalvariability. The dissolved oxygen in the surface water ranged from 5.93-9.64ml/L, while in the bottom water it ranged from 5.54-9.72 ml/L, and the oxygen saturation never fall below 94%. None thermal, the distribution of pH and content of oxygen, stratifications occurred. An apparent seasonal cycle of primary productivity was observed with remarkably high levels in the spring and fall, the lowest level in the winter. The range of net carbon assimilations showed 3.1-112.6 mgC/㎥/day or 15-427 mgC/㎡/day in spring, 37.0-271.2 mgC/㎥/day or 115-329 mgC/㎡/day in summer, 27.2-168.0 mgC/㎥ /day or 139-415 mgC/㎡/day in fall and 0.5-10.9 mgC/㎥/day or 5-19 mg/㎡/day in winter, respectively. Amount of chlorophyll ${\alpha}$ ranged from a minimum concentration of 0.2-0.3 mg/㎥ in the middle of February and a maximum 4.1-6.7 mg/㎥ in the middle of June. A general increase trend in chlorophyll ${\alpha}$ concentration was noted with increase of the river water temperature.

고리해역의 해양학적 특성 I. 수온, 염분, pH 및 투명도의 년간변화에 관하여

최상,정태화,Choe, Sang,Chung Tai Wha 한국해양학회 1971 韓國海洋學會誌 Vol.6 No.1

Observations of water temperature, salinity, pH and transparency of the Ko-ri sea area were made between May 1969 and April 1970. A seasonal thermocline was well defined in August, strongly isolating the warm serface water(19-22$^{\circ}C$) from the cold bottom water (14-17.5$^{\circ}C$) introducing from the open sea. In February the coldest isothermal water (11$^{\circ}C$) occurred. In the warm months(May- September), the salinity patterns show great variations with the coastal run-off During the cold months(December-April) the highest isohaline water (35 ) occurred. Annual ranges of surface and bottom pH values were 7.8-8.4(averaging 8.27) and 7.9-8.4(averaging 8.26), respectively. The transparency was greatest (6.0-7.0m) during winter and spring months and least (1.2-2.5m) during summer months.

낙동강 하구수역의 영양염류와 유기현탁물질

최상,정태화,Choe, Sang,Chung Tai Wha 한국해양학회 1972 韓國海洋學會誌 Vol.7 No.1

Seasonal changes in nutrients and suspended organic particulates were measured in the estuary of Nak-dong River in relation to the black laver bed. Monthly measurements of water temperature, pH, dissolved oxygen, nutrients ( $NH_4$-N, $NO_2$-N, $NO_3$\-N, $PO_4$-P and $SiO_2$-Si) and organic suspended particulates (organic carbon and nitrogen) were determined at five stations from February through December, 1970. PH varied 7.6-8.4 with an average of 8.0, and percent saturation of dissolved oxygen were 71-147% with an average of 100.8%. Studies gave evidence that Nak-dong River estuary is strongly enriched with nutrients. Concentrations of nutrients were: 0.13-12.54 ${\mu}g$-atoms/${\iota}$ (averaging 1.63 ${\mu}g$-atoms/${\iota}$) for $NH_4-N$, 0.12-2.09 ${\mu}g$-atoms/${\iota}$ (averaging 0.71 ${\mu}g$-atoms/${\iota}$) for $NO_2-N$, 3.46-56.79 ${\mu}g$-atoms/${\iota}$ (averaging 21.54 ${\mu}g$-atoms/${\iota}$) for $NO_3$-N, 4.04-57.90 ${\mu}g$-atoms/${\iota}$ (averaging 23.79 ${\mu}g$-atoms/${\iota}$) for total soluble nitrogen, 0.18-5.05 ${\mu}g$-atoms/${\iota}$ (averaging 0.96 ${\mu}g$-atoms/${\iota}$) for $PO_4$-P, and 18.33-133.29 ${\mu}g$-atoms/${\iota}$ (averaging 71.57 ${\mu}g$-atoms/${\iota}$) for $SiO_2$-Si, respectively. These nutrient levels were considerably higher compare with other productive laver beds of Wan do and Pyung-il Do in Korea or Ise Bay in Japan. Concentrations of suspended organic particulates varied 55-648 ${\mu}g/{\iota}$ (averaging 392 ${\mu}g/{\iota}$) with organic carbon, 30-155 ${\mu}g/{\iota}$ (averaging 92 ${\mu}g/{\iota}$) with organic nitrogen, and its carbon-nitrogen ratios were varied within 1.5-8.4 with an average of 4.6.

Vinblastine Determination Measured by a Sensitive ELISA Inhibition Assay

Jae-Wha Kim(김재화),Mi-Young Han(한미영),Hee-Gu Lee(이희구),Eun-Young Song(송은영),Tai-Wha Chung(정태화),Kyung-Soo Nam(남경수),In-Seong Choe(최인성),최용경 대한의생명과학회 1996 Biomedical Science Letters Vol.2 No.1

Vinblastine을 포함하는 bis-indole alkaloids에 대한 단일클론 항체를 생산하여 Vinca alkaloids의 양을 측정할 수 있는 간편한 immunoassay 체계를 확립하였다. Vinca alkaloids는 periwinkle식물체의 배양된 세포로부터 추출하여 BSA와 접합한 후 Balb/c생쥐에 면역시켜 얻은 비장세포와 골수종양세포의 융합을 유도하여 VBL-BSA에 반응하는 클론을 ELISA 방법으로 분석하였으며 이들 클론 중 bis-indole alkaloids와 특이적으로 반응하는 항체는 inhibition assay를 통하여 분리할 수 있었고 그 결과 두 개의 단일클론 항체를 형성하는 세포주(KN-1과 KN-2)를 확립하였다. KN-1의 경우 dimeric bis-indole alkaloids와는 상당한 교차반응을 나타낸 반면 monomeric bis-indole alkaloids와는 교차반응을 나타내지 않았으며 이 클론의 항체를 이용하여 배양된 세포 추출물에 포함된 Vinca alkaloids의 양을 측정한 결과 0.05 nM정도의 dimeric Vinca alkoloids까지도 측정할 수 있었다. Specific monoclonal antibodies(mAbs) against bis-indole alkaloids related to vinblastine were established to develop a simple and specific immunoassay system for the quantitation of Vinca alkaloids. Vinca alkaloids were extracted from tissue cultured cells of periwinkle plant (Vinca rosea L.). Spleen cells from Balb/c mice immunized with vinblastine-bovine serum albumin(VBL-BSA) conjugate as immunogen were fused with myeloma cells(Sp2/0-Ag.14) in the presence of polyethylene glycol. In the preliminary experiments, 32 clones which highly reacted with VBL-BSA conjugate were selected by ELISA(Enzyme-linked immunosorbent assay). These clones were further analyzed by inhibition assay of ELISA. The results obtained with two typical monoclonal antibodies, KN-1 and KN-2, were described. KN-1 exhibited considerable reactivities with soluble dimeric bis-indole alkaloids, whereas no cross reacted with monomeric bis-indole alkaloids. However KN-2 showed cross reactivity with mono- and di-meric bis-indole alkaloids. Furthermore, KN-1 was applied to the immunoassay system for determining the VBL amounts of in vitro cultured cell extracts. This assay system could detect dimeric vinca alkaloid as low as 0.05 nM.

한강의 영양염류 및 주요이온류의 년변화와 그 수질적고찰

최상,정태화,곽희상,Choe, Sang,Chung, Tai Wha,Kwak, Hi-Sang 한국해양학회 1968 韓國海洋學會誌 Vol.3 No.1

Nutrients and principal ions contents in downstream water of the Han River, near Seoul, measured at somewhat three week intervals over a period of two years were shown its characteristic seasonal cycles. The annual ranges of nutrients were 0.5-7.7 $\mu\textrm{g}$ at/L of ammonia, 0.12-0.90 $\mu\textrm{g}$ at/L of nitrite, 2.9-35.9 $\mu\textrm{g}$ at/L of nitrate, 8.8-42.0 $\mu\textrm{g}$ at/L of tatal soluble nitrogen, 0.09-0.57 $\mu\textrm{g}$ at/L of phosphorus, and 3.0-15.3 mg/L of silicate, and these were lower in spring and fall closely correlate with phytoplankton blooms and higher in winter except ammonia and silicate contents. Annual variations of BOD and COD were 0.10-2.14 ml/L, 0.88-6.82 mg/L, respectively. The ionic concentrations of Cl, Ca and Mg were not high of which Cl 5.0-13.1 mg/L, Ca 3.7-9.1 mg/L and Mg 1.5-10.4 mg/L of annual ranges. Fe ranged 18.5-82.8 $\mu\textrm{g}$/L, Mn 15-58 $\mu\textrm{g}$/L, Cu 0.3-4.7 $\mu\textrm{g}$/L annually In general, the Han River water is low in nutrients, mineral contents, and may be said not yet polluted, excellent quantity and quality of river water.

미립자 응집반응을 이용한 C-reactive Protein의 면역측정법에 관한 연구

김재화(Jae-Wha Kim),송은영(Eun-Young Song),이희구(Hee-Gu Lee),최용경(Yong-Kyung Choe),최명자(Myung-Ja Choi),김용호(Yong Ho Kim),최인성(In-Seong Choe),정태화(Tai-Wha Chung) 대한의생명과학회 1996 Biomedical Science Letters Vol.2 No.1

환자의 복수와 늑막액으로부터 p-diazonium phenylphosphorylcholine(DPPC) coupled Separose-4B affinity chromatography와 hydroxylapatite chromatography를 실시하여 C-reactive protein(CRP)를 분리, 제정하였다. 정제된 CRP를 토끼에게 면역화하여 항혈청을 얻고 affinity chromatography를 하여 면역항체(IgG)를 분리하였다. 분리된 면역항체를 미립자에 감작시킨 후 미립자 응집반응에 의하여 3분내에 CRP를 측정할 수 있는 간이 면역측정법을 개발하였다. 본 연구에서 개발된 CRP측정법의 검출범위는 0.5~20㎎/dl이며, 임상 시험 결과 0.7~2.9㎎/dl에서는 강한 응집반응을, 5.0~13.2㎎/dl에서는 약한 응집반응을 보였고 28㎎/dl이상에서는 항원 과잉으로 인한(zone of Ag excess phenomenon) 위음성을 나타냈다. 74명의 환자 혈청을 대상으로 CRP의 농도를 조사한 결과 평균치는 3.8㎎/dl이었으며 대부분의 환자에서는 10㎎/dl 이하의 농도로 존재하였다. 그러므로 1차판정시 음성을 나타낸 시료라도 혈청을 5~10배정도 희석하여 재분석한다면 오차없이 CRP를 검출할 수 있었다. 환자 혈청을 검체로 하여 본 연구에서 개발한 면역측정법과 현재 수입 시판중인 프랑스의 B사 제품과 일본의 I사 제품을 비교한 결과 좋은 상관관계를 보였다. 이와 같은 평가분석을 통하여 볼 때 본 연구에서 개발한 간이 면역측정법은 사용이 비교적 간편하며 신빙성이 있어 CRP를 스크리닝 하는데 효과적임을 알 수 있었다. The C-reactive protein(CRP) from ascitic and pleural fluid was purified using calcium dependent affinity chromatography of CNBr activated Sepharose-4B covalently coupled to p-diazonium phenylphosphorylcholine(DPPC) and hydroxylapitite chromatography. Polyclonal antibody was prepared from rabbit by immunizing the purified CRP. Specific immunoglobulin G was isolated using affinity chromatography and coupled to microparticles. A sensitive microparticle-based immunoassay was developed to measure CRP within 3 mins. The detection range was between 0.5㎎/dl and 20㎎/dl in serum, showing strong response in the range of 0.7~2.9 ㎎/dl, weak response in 5.0~13.2 ㎎/dl and zone phenomenon over 28㎎/dl. The average value of CRP in 74 samples was 3.8㎎/dl and most of the values were lower than 10㎎/dl. The CRP values of serum samples were determined by our microparticle-based immunoassay, and were compared with those obtained using the other commercial products(B Co., France and I Co., Japan). Good correlations were shown between the values obtained by our developed mi-croparticle-based immunoassay system and those by other commercial products. All performance characteristics evaluated make our developed microparticles-based immunoassay suitable for a simple, rapid, and reliable screening of CRP in serum.

방사면역학적 측적방법을 이용한 운동 선수들의 뇨중 T / E , T / LH 비율 측정 검사

최명자,강혜경,정태화,박종세,한문희 ( Myung Ja Choi,Hea Kyung Kang,Tai Wha Chung,Jon Gsei Park,Moon H . Han ) 생화학분자생물학회 1987 BMB Reports Vol.20 No.3

The studies reported that the urinary ratios of testosterone/epitestosterone (T/E) and testosterone/leutenizing hormone(T/LH) were increased significantly by the administration of anabolic steroids to normal subjects. Based on the report above, the T/E and T/LH ratios were investigated by a RIA method for the 194 urine specimens of the Asian Games atheletes. The normal distribution of the testosterone(T), epitestosterone(E), and leutenizing hormone(LH) values were established and the T/E and T/LH ratios were investigated for steroid doping control. The correlation study between the T/E and T/LH ratios indicates that the high T/E ratio is not necessarily associated with the high T/LH ratio within the population who have the normal values of the T/E and T/LH ratios. It was also found that the RIA performances of T and E assays were very sensitive and could quantitatively detect the T and E respectively without cross-reaction. The standard curve range covers a broad range of steroid values.

The Evaluation of T/E and T/LH Ratios in Athelete's Urine by the Radioimmunoassay Methods

최명자,강혜경,정태화,박종세,한문희,Choi, Myung-Ja,Kang, Hea-Kyung,Chung, Tai-Wha,Park, Jong-Sei,Han, Moon-H. 생화학분자생물학회 1987 한국생화학회지 Vol.20 No.3

Anabolic steroid를 정상인에게 투약하면 testosterone/epitestosterone(T/E) 비율과 testosterone/leutenizing hormone(T/LH) 비율은 상승된다고 보고된 바 있다. 위와같은 보고를 근거로, 1986년도 제 10회 아시아 경기에 입상한 선수 및 그 후보선수의 뇨를 검체로 하여 RIA를 이용한 testosterone(T)과 epitestosterone(E) leutenizing hormone(LH)의 정량분석을 수행했으며, T/E, T/LH의 정상치 및 그 분포도를 확립하였다. 또한 T/E와 T/LH의 상관관계를 조사한 결과 T/E와 T/LH가 정상범위에 속할 경우에는 T/E가 높은 값을 갖더라도 T/LH가 반드시 높은 것으로 나타나지는 않았다. 이번에 사용한 RIA방법은 강도가 매우 높고 광범위한 농도에 걸쳐서 교차 반응없이 T와 E를 각각 정량적으로 측정할 수 있음을 확인하였다. The studies reported that the urinary ratios of testosterone/epitestosterone(T/E) and testosterone/leutenizing hormone(T/LH) were increased significantly by the administration of anabolic steroids to normal subjects. Based on the report above, the T/E and T/LH ratios were investigated by a RIA method for the 194 urine specimens of the Asian Games atheletes. The normal distribution of the testosterone(T), epitestosterone(E), and leutenizing hormone(LH) values were established and the T/E and T/LH ratios were investigated for steroid doping control. The correlation study between the T/E and T/LH ratios indicates that the high T/E ratio is not necessarily associated with the high T/LH ratio within the population who have the normal values of the TIE and T/LH ratios. It was also found that the RIA performances of T and E assays were very sensitive and could quantitatively detect the T and E respectively without cross-reaction. The standard curve range covers a broad range of steroid values.