음양곽(淫羊藿) 열수 추출물의 Smad 신호 억제를 통한 간성상세포의 활성 조절

정지윤,민병구,박정아,변성희,조일제,김상찬,Jung, Ji Yun,Min, Byung-Gu,Park, Chung A,Byun, Sung Hui,Cho, Il Je,Kim, Sang Chan 대한한의학방제학회 2018 大韓韓醫學方劑學會誌 Vol.26 No.3

Objectives : In Traditional Korean Medicine, Epimedium koreanum Nakai has diverse pharmacological activities to treat impotence, forgetfulness, cataract and exophthalmos. Present study investigated anti-fibrogenic effects of E. koreanum water extract (EKE) in hepatic stellate cells (HSCs). Methods : To study anti-fibrogenic effects of EKE, LX-2 cells, a human immortalized HSCs, were pre-treated with $3-300{\mu}g/mL$ of EKE, and then subsequently exposed to 5 ng/mL of transforming growth $factor-{\beta}1$ ($TGF-{\beta}1$). Expression level of ${\alpha}-smooth$ muscle actin was determined by immunoblot analysis. Phosphorylation of Smad, transactivation of Smad, and expression of plasminogen activator inhibitor-1 (PAI-1) were monitored to investigate the effect of EKE on $TGF-{\beta}1-mediated$ signaling pathway. Results : Up to $100{\mu}g/mL$, EKE did not show any cytotoxicity on LX-2 cells. Pre-treatment of EKE ($100{\mu}g/mL$) significantly inhibited ${\alpha}-smooth$ muscle actin expression induced by $TGF-{\beta}1$. In addition, EKE significantly decreased Smad2 and Smad3 phosphorylations, Smad binding element-driven luciferase activity and PAI-1 expression by $TGF-{\beta}1$. Of three flavonoid compounds found in EKE, only quercertin ($30{\mu}M$) attenuated $TGF-{\beta}1-mediated$ PAI-1 expression. Conclusion : These results suggest that EKE has an ability to suppress fibrogenic process in HSCs via inhibition of $TGF-{\beta}1/Smad$ signaling pathway.

유방의 세침흡인 세포검사: 수기 액상세포검사법(Liqui-$PREP^{TM}$)과 고식적 도말법과의 비교

정지윤,김정식,김영수,김혜정,박지영,Jeong, Ji-Yun,Kim, Jeong-Shik,Kim, Young-Su,Kim, Hye-Jung,Park, Ji-Young 대한세포병리학회 2008 대한세포병리학회지 Vol.19 No.1

Fine needle aspiration (FNA) cytology of the breast is a useful method for diagnosing breast lesions. Yet making the definite diagnosis with performing FNA is limited by some problems, such as the low cellularity, the poor preservation and the obscuring background. Recent studies have found that liquid-based cytology solves such problems, but it is an expensive method and it is limited by the loss of the background information. The purpose of this study is to compare the Liqui-$PREP^{TM}$, a new manual liquid-based method of cytology, and the conventional smears for analyzing breast FNA cytology materials. A total of 31 randomized FNA specimens of breast were studied. In each case, both the conventional smears and the Liqui-$PREP^{TM}$ method were performed, and the smears were evaluated for cellularity, cellular preservation, the background, the cytologic features and the architectural arrangement. The cellularity and architectural arrangement were equal for both preparations. The Liqui-$PREP^{TM}$ specimens showed better cellular preservation, loss of the obscuring background, no overlapping of cells and a smaller area to screen compared with the conventional smears. Moreover, it has the potential advantages of being able to use the remaining specimens for immunohistochemical study and ploidy analysis, and it can reduce the costs for preparation compared with the other liquid-based methods of cytology. But some background information is lost in the Liqui-$PREP^{TM}$ specimens, the same as the other liquid-based methods of cytology. In conclusion, the Liqui-$PREP^{TM}$ and conventional smears showed good correlation, but they have their respective advantages and disadvantages. These results suggest that Liqui-$PREP^{TM}$ can contribute to making the accurate diagnosis with performing breast FNA cytology when it is used along with other methods.

천심련 물 추출물의 간세포보호효과

정지윤 ( Ji Yun Jung ),박정아 ( Chung A Park ) 대구한의대학교 제한동의학술원 2020 東西醫學 Vol.45 No.1

Andrographis Herba is used as a traditional herbal medicine in the southeast Asian countries for the treatment of stomatitis, pharyngalgia, fever etc. In this study, we investigated the hepatoprotective effect of water extract of Andrographis Herba (AHE; the lyophilized yield was 11.2%) on tert-butyl hydroperoxide (t-BHP)-induced oxidative stress in human hepatoma cell line HepG2. T-BHP is widely used to induce oxidative stress in a variety of cells. In HepG2, exposure to T-BHP results in excessive ROS generation and depletion of anti-oxidant systems. To investigate the hepatoprotective effect of AHE, cell viability was measured by MTT assay, apoptosis-related proteins, poly ADP ribose polymerase (PARP) and procaspase-3 were detected by immuno-blot assay and then analyzed the expression densities. In addition, the changes of H₂O₂ production was determined. Pre-treatment with two dosages of AHE (100 and 300 ㎍/㎖) significantly increased the cell viability, PARP and procaspase-3 reduced by t-BHP. In addition AHE also significantly decreased the oxidative stress in t-BHP-nduced HepG2 cells. These results suggest that AHE has hepatoprotective and anti-oxidant effect.

EU 난민정책 형성과정에서 집행위원회(European Commission)의 역할

정지윤(Ji Yun Jung),박성호(Sung Ho Park) 연세대학교 동서문제연구원 2017 동서연구 Vol.29 No.3

본 연구는 다층거버넌스 (multi-level governance, MLG)이론에 기반하여, 유럽연합(European Union, EU)의 난민정책 형성과정을 분석한다. 특히 EU 집행위원회(European Commission)가 정책결정에 미친 영향력에 초점을 두고, 이를 설명하는 초국적 인과메커니즘을 탐구한다. 핵심가설은 집행위원회가 EU회원국 및 이사회 기구에 정책결정의 영향력을 행사하는 데에, 해당 이슈영역의 권위적 국제기구인 UN난민기구(United Nations High Commissioner for Refugees, UNHCR)와의 초국적 정책연합이 중요 요인으로 작용했다는 것이다. 이러한 가설을 검증하기 위해 본 연구는 현 EU 난민정책의 근간이 형성된 2000년부터 2005년까지의 주요 정책의 도입과정을 살펴본다. This article applies multi-level governance theory to examine refugee policy making in the European Union (EU). The authors focus on the role of European Commission in the policy making process, identifying a transnational causal mechanism which explains the Commission s varying influence vis-a-vis member states and other EU institutions. The core argument is that the Commission s influence depended on whether or not it could build a transnational policy coalition with UNHCR(United Nations High Commissioner for Refugees), the most established international organization on refugee issues. The authors support their claim by examining major refugee policies, introduced during the 2000-2005 periods, which established the foundation of the EU refugee system.

국소 및 경구 스테로이드 제제의 사용과 연관된 인식도 비교 연구

정지윤 ( Ji Yun Jung ),신요섭 ( Yo Sup Shin ),조은별 ( Eun Byul Cho ),박은주 ( Eun Joo Park ),김광호 ( Kwang Ho Kim ),김광중 ( Kwang Joong Kim ) 대한피부과학회 2017 대한피부과학회지 Vol.55 No.4

Background: Topical and oral steroids are widely prescribed in dermatology. While interest in steroid use has increased rapidly, people have also acquired misinformation that causes rampant fears. Objective: To identify fears and misunderstandings about steroid use by comparing doctors` attitudes and treatment and non-treatment group experiences with steroid use. Methods: A total of 346 participants including outpatients who visited the Department of Dermatology at Hallym University Sacred Heart Hospital, physicians who work at the hospital, and visitors to its Department of Occupational and Environmental Medicine completed questionnaires. Results: The topical treatment group had a high response rate of 46.7% regarding feeling “somewhat anxious” about using topical steroids, while the oral treatment group had a high response rate of 53.8% regarding feeling “somewhat anxious” about using oral steroids. With respect to information about the adverse effects of steroid use, those in the non-experience group obtained their information mostly from the Internet (67.5%) or the media (47.5%), whereas those in the topical treatment group obtained their information from product guides (48%) and doctors (38.7%). Meanwhile, the oral treatment group acquired information from acquaintances (39.7%) or doctors (32.1%). Conclusion: Dermatologists must evaluate the validity of the information that patients have about the adverse effects of topical and oral steroid use as well as the resulting phenomenon of steroid phobia. If the information possessed by patients is inaccurate, clinicians must provide a full understanding and proper education to enhance patient satisfaction and treatment compliance and success rates. (Korean J Dermatol 2017;55(4):227∼236)

통일교육시범학교와 일반학교 통일교육 비교 연구

정지윤 ( Ji Yun Jung ),김재기 ( Jai Gi Kim ) 조선대학교 동북아연구소(구 통일문제연구소) 2014 동북아연구 Vol.29 No.1

학교통일교육은 통일시대를 이끌어나갈 주역인 학생들에게 올바른 통일의식을 심어주고, 통일문제를 객관적으로 판단할 수 있는 능력을 갖도록 하는 교육 과정이다. 학교통일교육은 독립적인 교과로 존재하는 것이 아니라 다양한 교과목에 일부내용이 포함되어 있으며 범교과적인 특징이 있다. 통일교육협의회, 서울대 통일평화연구원, 한국교육개발원 등이 시행한 연구에서는 10대 청소년들의 통일의식이 낮아지고 있다고 보고하고 있다. 따라서 앞으로 통일한국의 주역으로서 청소년을 대상으로 한 통일교육이 시급하며 이를 위해서는 청소년들에게 교육의 접근성이 가장 용이한 학교 통일 교육을 강화할 필요가 있다. 이 연구는 경기도 파주시 지역에 위치한 통일교육시범학교(experimental group)와 비시범학교(control group) 학생들의 통일 의식의 차이를 비교해보았다. ‘통일교육에 대한 인식’에 있어서 통일교육시범학교가 비시범학교에 비해 현재 학교에서의 통일교육이 유익하다고 생각하는 학생들이 더 많았고, 통일교육의 방식에 대해서도 만족하는 학생들이 더 많았으며, 통일교육이 북한 및 통일에 대해 이해하는데 도움이 되었다고 생각하는 학생이 더 많은 것으로 나타났다. 학교에서의 통일교육에 대한 필요성에 대해 통일교육시범학교 학생들보다 비시범학교 학생들이 그 필요성을 더 많이 느끼고 있었다. 또한 비시범학교 학생들에 비해 통일교육시범학교 학생들이 통일교육에 대해 더 유익하다고 느끼고 있었다. 이는 통일교육시범학교가 비시범학교에 비해 통일교육을 더 효과적으로 실시하고 있는 것을 알 수 있다. Above all, the view on North Korea and Korean unification for teenagers who will be the leaders of a unified Korea is very important. According to the ‘2013 Korean unification survey of teenagers’, lots of teenagers still have bad impressions of North Korea. The 2012 survey, from a unification and peace researcher at Seoul National University, also shows the younger generation have lower consciousness about unification. This research tells us unification education is significant and should be reinforced, especially in school, where the best access to teenagers is. Therefore, focusing on management of the unification model school, rather than extension and strengthening of unification education in regular schools, this study tried to find what different points of view about Korean unification students have between the unification model school and regular school. In terms of perception of unification education, the model school students receive lecture instruction, audio-visual education, discussion courses, field study and North Korean defector guest lectures about North Korea and unification many times, compared with the non-model school students. The survey suggests that these teaching methods are helpful to unification education. Therefore, there is a significant difference between the model school students and the non-model school students about awareness of unification, attitude towards North Korea and perception of unification education. That is, the model school students are more widely informed about North Korean unification than the non-model school students. Additionally, the model school students more often feel the need for unification than the non-model school students. Operation of the model school for unification education paid off by promoting proper unification perception.

Growth of 2-Year-Old Ginseng and the Contents of Ginsenoside according to Cultivation Type

Ji Yun Jung(정지윤),Jin Kook Choi(최진국),Yang Ae Choi(최양애),Myeong Hwan Jang(장명환),Do Yeon Won(원도연),Im Soo Kim(김임수) 한국약용작물학회 2017 한국약용작물학회 학술대회논문집 Vol.2017 No.2

조기위암의 점막하박리술 후 발생한 위주변 농양

정지윤 ( Ji Yun Jung ),김준성 ( Joon Sung Kim ),김병욱 ( Byung Wook Kim ),박성민 ( Sung Min Park ),김기준 ( Gi Jun Kim ),류승지 ( Seung Ji Ryu ),김영욱 ( Young Wook Kim ),지정선 ( Jeong Seon Ji ),최황 ( Hwang Choi ) 대한소화기학회 2016 대한소화기학회지 Vol.67 No.3

Endoscopic submucosal dissection (ESD) for early gastric cancer (EGC) is a widely accepted and well established procedure because of its curative potential and low invasiveness compared with surgery. Perforation is a potential major complication during ESD, and non-surgical treatments such as endoscopic closure with clips are sufficient in most cases. Here, we report a case of perigastric abscess that occurred as a complication of ESD for EGC. The patient improved with administration of antibiotics without surgical intervention. (Korean J Gastroenterol 2016;67:142-145)

통일교육시범학교와 일반학교 통일교육 비교 연구

정지윤 ( Ji Yun Jung ) , 김재기 ( Jai Gi Kim ) 조선대학교 동북아문제연구소 2014 동북아연구 Vol.29 No.1

Above all, the view on North Korea and Korean unification for teenagers who will be the leaders of a unified Korea is very important. According to the ‘2013 Korean unification survey of teenagers’, lots of teenagers still have bad impressions of North Korea. The 2012 survey, from a unification and peace researcher at Seoul National University, also shows the younger generation have lower consciousness about unification. This research tells us unification education is significant and should be reinforced, especially in school, where the best access to teenagers is. Therefore, focusing on management of the unification model school, rather than extension and strengthening of unification education in regular schools, this study tried to find what different points of view about Korean unification students have between the unification model school and regular school. In terms of perception of unification education, the model school students receive lecture instruction, audio-visual education, discussion courses, field study and North Korean defector guest lectures about North Korea and unification many times, compared with the non-model school students. The survey suggests that these teaching methods are helpful to unification education. Therefore, there is a significant difference between the model school students and the non-model school students about awareness of unification, attitude towards North Korea and perception of unification education. That is, the model school students are more widely informed about North Korean unification than the non-model school students. Additionally, the model school students more often feel the need for unification than the non-model school students. Operation of the model school for unification education paid off by promoting proper unification perception. 학교통일교육은 통일시대를 이끌어나갈 주역인 학생들에게 올바른 통일의식을 심어주고, 통일문제를 객관적으로 판단할 수 있는 능력을 갖도록 하는 교육 과정이다. 학교통일교육은 독립적인 교과로 존재하는 것이 아니라 다양한 교과목에 일부내용이 포함되어 있으며 범교과적인 특징이 있다. 통일교육협의회, 서울대 통일평화연구원, 한국교육개발원 등이 시행한 연구에서는 10대 청소년들의 통일의식이 낮아지고 있다고 보고하고 있다. 따라서 앞으로 통일한국의 주역으로서 청소년을 대상으로 한 통일교육이 시급하며 이를 위해서는 청소년들에게 교육의 접근성이 가장 용이한 학교 통일 교육을 강화할 필요가 있다. 이 연구는 경기도 파주시 지역에 위치한 통일교육시범학교(experimental group)와 비시범학교(control group) 학생들의 통일 의식의 차이를 비교해보았다. ‘통일교육에 대한 인식’에 있어서 통일교육시범학교가 비시범학교에 비해 현재 학교에서의 통일교육이 유익하다고 생각하는 학생들이 더 많았고, 통일교육의 방식에 대해서도 만족하는 학생들이 더 많았으며, 통일교육이 북한 및 통일에 대해 이해하는데 도움이 되었다고 생각하는 학생이 더 많은 것으로 나타났다. 학교에서의 통일교육에 대한 필요성에 대해 통일교육시범학교 학생들보다 비시범학교 학생들이 그 필요성을 더 많이 느끼고 있었다. 또한 비시범학교 학생들에 비해 통일교육시범학교 학생들이 통일교육에 대해 더 유익하다고 느끼고 있었다. 이는 통일교육시범학교가 비시범학교에 비해 통일교육을 더 효과적으로 실시하고 있는 것을 알 수 있다.

건전한 카지노 문화의 정착을 위한 도박중독과 충동성에 관한 이해

정지윤(Ji Yun Jung),황현국(Hyun-kuk Hwang) 한국엔터테인먼트산업학회 2016 한국엔터테인먼트산업학회 학술대회 논문집 Vol.2016 No.5

본 연구는 도박 고위험군을 미리 파악하고자 정상적이라 여겨지는 대학생 집단을 대상으로 충동성과 관련된 성격유형의 특징이 도박에 미치는 영향을 알아보고, 그리고 도박집착이 조절실패를 거쳐 궁극적으로 중요관계손상에 미치는 영향관계를 조사하였다. 실증분석에 따른 본 연구의 결론 및 시사점은 다음과 같다. 첫째, 인지충동성, 운동충동성 및 무계획충동성으로 구분된 충동성의 특징들은 도박집착과의 영향관계에서 유의한 영향을 미치지 못하는 것으로 나타났다. 이러한 분석결과는 충동성의 성격특성이 도박집착이라는 도박행위과정에 뚜렷한 영향을 미치는 요인이 될 수 없다는 것을 시사한다. 둘째, 도박행위과정에서 도박집착이 도박조절실패에 미치는 영향력은 39.4%이었고 유의적인 영향을 미치는 것으로 나타났다. 이러한 분석결과는 도박행위가 반복되거나 습관화될 경우, 결과적으로 통제력상실과 같은 도박중독의 경과를 보여줄 수 있음을 시사한다. 셋째, 도박행위과정에서 도박집착 및 도박조절실패가 중요관계손상에 미치는 직접효과는 각각 33.1%, 19.4%로 유의적인 영향을 미치는 것으로 나타났다. 또한 도박집착이 도박조절실패를 통해 중요관계손상에 미치는 간접효과는 7.6%로 나타나 중요관계손상에 대한 도박집착의 영향력은 총 40.8% 이었다. 이러한 분석결과는 도박집착이 직접적으로, 또는 통제력상실과 같은 도박중독의 과정을 거쳐 중요관계로의 손상으로 이어질 수 있음을 시사한다. 따라서 본 연구결과는 도박중독의 위험성뿐 아니라 도박의 폐해로 고통을 경험할 가능성이 높은 습관적 또는 장기 카지노 출입고객에 대해서는 캠페인 또는 중독관련 치료프로그램을 통해 도박 문제에 대한 심각성을 일깨우고, 변화를 유도할 필요성을 제기하고 있다.