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정상과 비정상핵형을 가진 인간 배아줄기세포의 비교 분석
설혜원 ( Hye Won Seol ),백진아 ( Jin Ah Baek ),정주원 ( Ju Won Jung ),윤보애 ( Bo Ae Yoon ),김희선 ( Hee Sun Kim ),오선경 ( Sun Kyung Oh ),구승엽 ( Seung Yup Ku ),김석현 ( Seok Hyun Kim ),문신용 ( Shin Yong Moon ),최영민 ( Young M 서울대학교 인구의학연구소 2011 人口醫學硏究論集 Vol.24 No.-
Human embryonic stem cells (hESCs) are a useful source for biological research fields and applications in regenerative medicine. The maintenance and distribution of the undifferentiated hESCs of normal karyotypes are very important. The changes of colony morphology were observed during the culturing of hESCs, and chromosomal analysis was performed in this study. Abnormal karyotypes were identified in the cells which have the changes of the colony morphology. Attachment ratio and the spontaneous differentiation ratio of abnormal cells were compared to those of normal cells. High cell attachment ratio and low spontaneous differentiation ratio were confirmed in karyotypically abnormal cells. HESCs showed chromosomal anomaly if the colony morphology changed, and high attachment ratio and low spontaneous differentiation ratio have been observed during the cell culture. Our results suggest that the morphology of the colonies would play a ro1e as a criterion for chromosomal anomaly.
Hypoxia-induced Angiogenesis during Carcinogenesis
최규실,배문경,정주원,문효은,김규원,Choe, Gyu-Sil,Bae, Mun-Gyeong,Jeong, Ju-Won,Mun, Hyo-Eun,Kim, Gyu-Won Korean Society for Biochemistry and Molecular Biol 2003 Journal of biochemistry and molecular biology Vol.36 No.1
The formation of new blood vessels, angiogenesis, is an essential process during development and disease. Angiogenesis is well known as a crucial step in tumor growth and progression. Angiogenesis is induced by hypoxic conditions and regulated by the hypoxia-inducible factor 1 (HIF-1). The expression of HIF-1 correlates with hypoxia-induced angiogenesis as a result of the induction of the major HIF-1 target gene, vascular endothelial cell growth factor (VEGF). In this review, a brief overview of the mechanism of angiogenesis is discussed, focusing on the regulatory processes of the HIF-1 transcription factor. HIF-1 consists of a constitutively expressed HIF-1 beta(HIF-1β) subunit and an oxygen-regulated HIF-1 alpha(HIF-1α) subunit. The stability and activity of HIF-1α are regulated by the interaction with various proteins, such as pVHL, p53, and p300/CBP as well as by post-translational modifications, hydroxylation, acetylation, and phosphorylation. It was recently reported that HIF-1α binds a co-activator of the AP-1 transciption factor, Jab-1, which inhibits the p53-dependent degradation of HIF-1 and enhances the transcriptional activity of HIF-1 and the subsequent VEGF expression under hypoxic conditions. ARD1 acetylates HIF-1α and stimulates pVHL-mediated ubiquitination of HIF-1α. With a growing knowledge of the molecular mechanisms in this field, novel strategies to prevent tumor angiogenesis can be developed, and form these, new anticancer therapies may arise.
결핵 환자에서 Rifampin에 의한 Henoch-Shonlein Purpura
김혜련 ( Hye Ryun Kim ),박병훈 ( Byung Hoon Park ),손지영 ( Ji Young Son ),정지예 ( Ji Ye Jung ),안정련 ( Jung Ryun Ahn ),정윤숙 ( Yoon Suk Jung ),임주은 ( Ju Eun Lim ),정주원 ( Ju Won Jung ),문지애 ( Ji Ae Moon ),변민광 ( Min Kwa 대한결핵 및 호흡기학회 2008 Tuberculosis and Respiratory Diseases Vol.65 No.2
Rifampin is one of the first line drugs for treating tuberculosis, but it might be associated with serious adverse effects, including renal failure. We report here on a case of a 57-year-old patient who developed Henoch-Shonlein purpura during antituberculosis therapy that included rifampin. The patient converted to negative on the AFB smear for tuberculosis two weeks after the initial administration of antituberculosis medication. After treatment for 60 days, this patient was diagnosed with Henoch-Shonlein purpura by the purpura lesion on the lower legs, the leukocytoclastic vasculitis, the renal impairment and the pathological examination. After stopping rifampin, the skin lesions disappeared in about 10 days and his renal function gradually improved. This case study showed that Henoch-Schonlein purpura can be caused by rifampin during antituberculosis therapy and we recommend that the use of rifampin should be restrained when clinical symptoms of Henoch-Shonlein purpura are observed. (Tuberc Respir Dis 2008;65:116-120)
초기 계대 인간 배아줄기세포의 해동 후 효율적인 배양 방법
백진아,김희선,설혜원,서진,정주원,윤보애,박용빈,오선경,구승엽,김석현,최영민,문신용,Baek, Jin-Ah,Kim, Hee-Sun,Seol, Hye-Won,Seo, Jin,Jung, Ju-Won,Yoon, Bo-Ae,Park, Yong-Bin,Oh, Sun-Kyung,Ku, Seung-Yup,Kim, Seok-Hyun,Choi, Young-Min,Moon, 대한생식의학회 2009 Clinical and Experimental Reproductive Medicine Vol.36 No.4
목 적: 인간 배아줄기세포 (human embryonic stem cells; hESCs)는 미분화 상태로 무한 증식할 수 있는 자가 증식(self-renewal) 능력과 인체의 모든 세포로 분화할 수 있는 전분화능 (pluripotency)의 특징을 가진 세포로, 손상된 세포를 건강한 세포로 대체하고자 하는 세포치료 (cell therapy) 연구에 활용하기 위한 세포 공급원 (cell source)으로 제시되고 있다. 그러나 인간 배아줄기세포는 확립된 초기에 세포를 안정적으로 배양하고 유지하는 과정이 쉽지 않으며, 특히 동결보존되어 있던 세포를 해동한 후 배양할 때 자연 발생적 분화가 높기 때문에 세포주의 유지에 많은 어려움이 따른다. 본 연구에서는 동결보존되어 있던 초기 계대의 인간 배아줄기세포를 해동하여 다시 배양할 때 자연 발생적 분화 부분을 기계적 분리 방법으로 제거하여 미분화 상태의 세포를 보다 빠르게 확보하기 위한 효율적인 방법에 대해 알아보고자 하였다. 연구방법: 인간 배아줄기세포를 계대 배양한지 4일이 되는 날, 50% 이상의 자연 발생적 분화가 나타난 세포군에서 분화된 부분만을 절개용 유리 피펫 (drawn-out dissecting pasture pipette)을 사용하여 기계적인 방법으로 제거하였다. 이후 지속적으로 배양액을 교환해 주며 세포군의 제거된 부분을 7일째 되는 날까지 관찰하였다. 결 과: 기계적 분리 방법을 사용하여 인간 배아줄기세포의 자연 발생적인 분화 부분을 제거한 빈 공간에 미분화상태의 인간 배아줄기세포가 분열하여 채워지는 것을 관찰하였다. 또한, 이 실험 방법을 연속 두 번 적용하여 배양했을 때 미분화 세포로 회복되는 세포군의 비율이 조금 더 높아지는 것을 확인할 수 있었다. 결 론: 동결되어 있던 초기 계대 인간 배아줄기세포의 해동 후, 자연 발생적 분화에 의해 미분화 상태를 유지하는 세포의 수가 적어 계대를 유지 하기가 어려울 때 이와 같은 기계적 분리 방법을 사용하여 자연 발생적 분화 부분을 제거한 후 배양을 지속하는 것이 단기간 내에 미분화 상태를 유지하는 인간 배아줄기세포의 양적 확보를 위한 효율적인 방법이라고 사료된다. Objective: Human embryonic stem cells (hESCs) have the capacity to differentiate into all of the cell types and therefore hold promise for cell therapeutic applications. In order to utilize this important potential of hESCs, enhancement of currently used technologies for handling and manipulating the cells is required. The cryopreservation of hESC colonies was successfully performed using the vitrification and slow freezing-rapid thawing method. However, most of the hESC colonies were showed extremely spontaneous differentiation after freezing and thawing. In this study, we were performed to rapidly collect of early passage hESCs, which was thawed and had high rate of spontaneously differentiation of SNUhES11 cell line. Methods: Four days after plating, partially spontaneously differentiated parts of hESC colony were cut off using finely drawn-out dissecting pipette, which is mechanical separation method. Results: After separating of spontaneously differentiated cells, we observed that removed parts were recovered by undifferentiated cells. Furthermore, mechanical separation method was more efficient for hESCs expansion after thawing when we repeated this method. The recovery rate after removing differentiated parts of hESC colonies were 55.0%, 74.5%, and 71.1% when we have applied this method to three passages. Conclusion: Mechanical separation method is highly effective for rapidly collecting and large volumes of undifferentiated cells after thawing of cryopreserved early passage hESCs.
무인 시스템을 탑재한 다기능 농업 미니로봇의 개발에 관한 연구
김지환 ( Ji-hwan Kim ),김강민 ( Kang-min Kim ),정지욱 ( Ji-wook Jung ),박진혁 ( Jin-hyeok Park ),정주원 ( Ju-won Jung ) 한국정보처리학회 2017 한국정보처리학회 학술대회논문집 Vol.24 No.2
지금의 농산업 기술발전에서 발생하는 사회적 갈등을 방지 할 수 있는 기술혁신의 일종으로 무인 시스템을 탑재한 미니로봇의 개발에 대해 연구한다. 해당 로봇은 모바일 5축 매니퓰레이터 형태를 기반으로 하여 험지에서 이동하기 위해 궤도바퀴를 이용하였고 웹 서버와 자율주행 기술로 일련의 무인 시스템을 구축하였다.