Further characterization of the causative virus of rabbit viral hepatitis, so-called rabbit haemorrhagic disease in Korea

정종식,정규식,이차수,신태균,Jyeong, Jong-sik,Jeong, Kyu-sik,Lee, Cha-soo,Shin, Tae-kyun The Korean Society of Veterinary Science 1992 大韓獸醫學會誌 Vol.32 No.3

국내에서 발생한 토끼 바이러스성 간염 소위 토끼 출혈병의 원인 바이러스를 감염토끼의 간조직으로 부터 분리정제한 후 바이러스의 핵산과 구성 단백질의 특징을 관찰하였던 바 다음과 같은 결과를 얻었다. 토끼간염바이러스는 분자량이 약 54 kilodalton인 한개의 구조단백을 가진 RNA 바이러스이며 바이러스 핵산의 크기는 약 7.5 kilobases로 나타났고 바이러스의 RNA는 배양세포에서는 감염을 일으키지 않았다. 바이러스 구성단백의 양상과 핵산의 크기 등을 종합해 볼 때 토끼의 간염 바이러스는 Caliciviridae에 속하는 것으로 간주된다. The causative virus causing rabbit hepatitis has been further characterized by evaluating viral proteins and viral nucleic acids of purified viruses from the liver of the experimentally infected rabbits. Rabbit hepatitis virus has one major structural protein of 54 kilodaltons and some minor proteins. Vrial RNA was resistant to DNAse I. The size of viral nucleic acid of this virus was calculated to be about 7.5 kilobases. These findings indicate that rabbit hepatitis virus belongs to the family Caliciviridae.

A pathological observation on the experimental rabbit viral hepatitis

정종식,권영란,이차수,신태균,Jyeong, Jong-sik,Kwon, Young-ran,Lee, Cha-soo,Shin, Tae-kyun The Korean Society of Veterinary Science 1992 大韓獸醫學會誌 Vol.32 No.4

This paper dealt with the clinical and pathological observations on the experimental rabbit viral hepatitis. Rabbits with 2-14 months of ages were intramuscularly inoculated with virus suspension. The results were summarized as follows. Ninety percents of experimental rabbits inoculated with virus died within 96 hours postinoculation. Common clinical signs were inappetence, increase in body temperature, depression, mild diarrhea and in three cases, bloody foam from nostrils was recognized. At necropsy, in most of the experimental cases, there were hyperemia or haemorrhages in many organs and pale liver. Intestinal catarrh and retention of turbid urine in urinary bladder were seen in some cases. Histopathologically, liver necrosis was found in all the cases died of this disease. However, there was a difference in the severity of hepatic necrosis. Haemorrhages were Iecognized in lungs, heart, liver, spleen, kidneys and thymus, in order. Liver necrosis was marked even in the cases with no haemorrhage. Perivascular cuffing in brain and catarrhal enteritis in small intestine were seen in many cases. From these results, consistent and primary lesion in this viral disease is hepatitis in susceptible rabbits. It was concluded that rabbit hepatitis virus might have the properties of hepatotropism and consequently induce peripheral necrosis in the liver leading to acute viremia with haemorrhages. 국내에서 발생된 토끼 virus성간염의 병리학적 소견을 명확히 규명하기 위하여 2~14개월령의 재래토끼에 간염에 이환된 토끼의 간조직 유제를 접종한후 임상 및 병리학적으로 관찰하였던 바 다음과 같은 결과를 얻었다. 접종한 30마리중 90%인 27마리가 감염되어 접종후 96시간이내에 폐사되었고 2~3월령 3두는 감염이 일어나지 않았다. 임상증상으로는 식욕부진, 체온상승, 연변 등이 나타났고 3두에서는 비강내로부터 혈액성 포말이 관찰되었다. 부검소견으로는 장기의 충출혈, 간장의 변성이 전예에서 관찰되었고 소장의 카타르성 장염과 방광내 혼탁한 뇨충만도 관찰되었다. 광학현미경적 소견으로는 간괴사가 정도의 차이가 있기는 하나 폐사된 27두 전예에서 관찰되었고, 출혈빈도는 폐가 가장 많았고 심장, 간장, 비장, 신장 및 흉선 순으로 나타났다. 한편 출혈이 관찰되지 않은 예에서도 간의 괴사소견은 현저하였다. 소장의 카타르성 장염과 뇌의 혈관주위 임파구 침윤도 관찰되었다. 이상의 결과를 종합해 볼 때 본 질병에서 출혈소견은 개체에 따라 변화가 심하였으나 중요한 소견은 간염으로 나타났고 원인 virus는 일차적으로 간에 친화성을 가지며 동시에 viremia를 일으켜 전신장기에 출혈을 야기하는 것으로 추정된다.

사라리 축우페사의 원인에 관한 연구

정종식 ( Jong Sik Jyeong ),박노찬 ( No Chan Park ),김정화 ( Jung Hwan Kim ),김영환 ( Young Hoan Kim ),조광현 ( Kwang Hyun Cho ),조민희 ( Min Hee Cho ),손재권 ( Jae Kweon Son ),김영욱 ( Young Wook Kim ) 한국가축위생학회 1999 韓國家畜衛生學會誌 Vol.22 No.4

This study was conducted to investigate the epidemiological, clinicopathological, microbiological, pathological observations and other tests from sudden death in feedlot cattle at the region of Sarari in Korea during the period from 1994 to 1999. Massive or sporadic occurrence of sudden death has been observed in 101 heads of 47 farmhouse. There were 20.8% in spring, 29.7% in summer, 16.8% in autumn, 32.7% in winter, and 62.3% in reproductive, 27.7% in growing, 5.0% in beef cattle, 5.0% in calf in prevalence of sudden death in cattle. Enterotoxemia(88.0%), pneumonia(3.5%), intestinal diarrhea(3.5%), liver abscess(1.5%) and indigestion(1.5%) were detected from 67 heads of sudden death cattle. In clinical observations, cattle were generally died of sudden recumbency with convulsions followed anorexia, depression, ataxia, muscular tremor, tachycardia and dyspnea without any premonitory symptoms. Epidemiological surveys showed no evidence that other factors such as pesticide, insecticide, fertilizer, chemical drugs and those of others caused sudden death. Macroscopically, there were coagulation disorders of blood, congestion, edema and haemorrhage of lung, congestion and haemorrhages, watery and blood-tinged contents of small intestine. Histopathologically, we observed pulmonary congestion and haemorrhage, necrotic intestinal mucosa accompanied with haemorrhage and congestion, and also increased globule leukocytes between bronchial epithelia with mild pneumonia. Clinicopathologically, only elevation of blood glucose and aspartate aminotransferase (AST) was detected. Magnesium and calcium deficiency were not detected, but parasites were detected highly in normal and dead cattles. Microbiologically, Clostridium(Cl) perfringens were detected from small intestinal contents of 94%(63/67) of sudden death cattle and 51%(51/101) of slaughter cattle, and the population were 10(6-8)cfu/mℓ after 16~32 hours. Consequently, it was proved that the cause of death in cattle was enterotoxemia. Pathogenic test of mouse and goat inoculated with Cl perfringens type A toxin has been demonstrated as similar observation to natural cases. In antimicrobial susceptibility test, ampicillin, bacitracin, polymycin, cephalothin, penicillin, choramphenicol, erythromycin, tetracycline were highly susceptible, and amikacin, gentamicin, kanamycin, neomycin, streptomycin, sulfamethoxine, sulfamethazine were resistant. Cl perfringens were resisted for 4 hours in 3% formalin, 20 minutes in 4% phenol, 20 minutes in 05% mercuric chloride and 40 minutes in 0.1% sodium hydroxide, respectively. The useful method to prevent from occurrance of enterotoxemia in feedlot cattle was a dietary administration of antibiotics and miyari acid.

동물(動物)바이러스의 새로운 분류법(分類法)

정종식 ( Jong Sik Jyeong ) 한국가축위생학회 1996 韓國家畜衛生學會誌 Vol.19 No.1

경북지역에서 수거된 식육의 Listeria sp 오염실태 조사

조종숙 ( Jong Suk Cho ),김유정 ( Yoo Jung Kim ),김성숙 ( Seong Suk Kim ),도재철 ( Jae Cheul Do ),김석한 ( Suk Hwan Kim ),이창우 ( Chang Woo Lee ),김인성 ( In Sung Kim ),정종식 ( Jong Sik Jyeong ) 한국가축위생학회 2000 韓國家畜衛生學會誌 Vol.23 No.4

Nowadays we continue to face challenges to the safety of our foods. It seems clear that contaminated meats provide the major route of listeria monocytogenes transmission from the environment to humans. L monocytogenes is the most important species associated with disease in humans among the listeria sp. The current incidence of symptomatic listeriosis caused by L morzocytogenes is uncertain. Although the number of reports in the literature on listeriosis are increasing, it is likely that they are actually unrecognized or underreported because of a lack of awareness on the part of some laboratory workers who fail to isolate or identify these organisms. To get the information of sanitary development, we survey various meats (beef, pork, etc) in Kyongbuk area. Samples were collected from local meat shop at Kyongbuk area. Total sixty six case were isolated and identified from one hundreds and seven samples. L innocua was the most abundant in listeria sp. Among 66 isolates, the number of isolated L innocua was 43(65.2%). The numbers of isolated L murrayi, L welshimeri, L monocytogenes and L seeligeri were 12 (18.2%), 7 (10.6%), 3 (4.5%) and 1 (1.5%), respectively, but L grayi, L ivanovii were not isolated.

간질감염(肝蛭感染) 및 구충제(驅蟲劑) DS-6 부작용(副作用)에 관(關)한 조사연구(調査硏究)

곽수동,정종식,조용준,Kwak, Soo-Dong,Jyeong, Jong-Sik,Cho, Yong-Joon 대한수의학회 1983 大韓獸醫學會誌 Vol.23 No.2

A servey on bovine fascioliasis in 52,876 cattle of Euiseong, Andong, Yecheon, Wolseong areas in Gyeongbug Province was carried out by the intradermal test, and the clinical side action of the positive cattle administered with DS-6 intramuscularly was observed. In addition pathological examination of 2 cattle died with side action of DS-6 was also conducted. The results of the studies are summarized as follows; 1. The average infestation rate of bovine fascioliasis was 31.1% and regional infestation rates were 35.1% in Eiseong, 34.5% in Yecheon, 32.2% in Andong, 26.9% in Wolseong, respectively. 2. The side actions were occurred in 1.5%(115/7,667) cf cattle administered with DS-6 and the clinical observations were salivation, dyspnea, hyperamia of conjunctiva, lacrimation, perspiration, urticaria, tremor, diarrhea, staggering, convulsion, edema and abortion. 3. Within 1 hour after the injection, side actions were occurred in 61.7% of the 115 cattle while they were observed in 37.4% of the 115 catte from 1 hour to 2 hours post injection. 4. The duration of the side action was less than 2 hours in 29.5%, 2 to 10 hours in 47.0% and more than 10 hours in 23.5% of the 115 cattle. 5. Autopsy findings and histopathological observations indicate that the death of cattle is caused by anaphylactic shock.

신생자돈(新生仔豚)의 선천성(先天性) 소면구증례(小眠球症例)

곽수동,정종식,권헌일,조용준,이차수,Kwak, Soo-dong,Jyeong, Jong-sik,Kwun, Hyun-ik,Jo, Yong-joon,Lee, Cha-soo 대한수의학회 1984 大韓獸醫學會誌 Vol.24 No.1

This study was undertaken to investigate congenital microphthalmia occurred at a pig farm in Gyeongbug province. These microphthalmic piglets composed of 20 piglets farrowed by three different sows(1, 11 and 8 piglets by No.1, 2 and 3 sow, respectively) that were apparently normal during pregnancy and after parturition. Attempts were made to know epidemiological findings and histopathological changes of these piglets and the results were summarized as follows; 1. One piglet from No. 1 sow exhibited staggering and incoordination and remained alive, but 19 piglets from No. 2 and 3 sow died between 3 and 20 minutes after birth. 2. In histopathological finding, hypoplasia and edema of all organs were observed in all piglets, and significant of lesions related to infection of microrganisms were not observed. 3. The cause of microphthalmia was considered as hypovitaminosis A in the sown intaked only barley barn before and during early pregnancy.

닭 Leucocytozoon병(病)의 집단발생(集團發生)과 병리학적(病理學的) 관찰(觀察)

곽수동,정종식,Kwak, Soo-Dong,Jyeong, Jong-Sik 대한수의학회 1983 大韓獸醫學會誌 Vol.23 No.2

The clinical and histopathological observation of leucocytozoonosis was carried out during July, 1982 at a poultry compound in Gyeongbuk province and the results were summarized as follows: 1. Leucocytozoonosis was observed in 36,900 of 59,900 chickens (61.6%) and the mortality rate was 18.3% (6,760 of 36,900 chickens infected). 2. Clinical findings were anorexia, dyspnea, paleness of comb, greenish diarrhea and reduction of egg production. 3. Gross lesions were yellowish white spots scattered on parenchymal organs and swelling of liver and spleen. In addition, petechiation and ecchymosis of small intestine, egg yolk and subcutaneous tissues were observed. 4. Histological findings were infiltration of mononuclear cells in liver, heart and lung, appearance of megaloschizonts in heart, liver, proventriculus and pancreas, proliferation of foreign body giant cells in heart, and hyperemia and swelling of all parenchymal organs.

토끼의 바이러스성 간염(肝炎)에 관한 연구(硏究) II. 실험적(實驗的) 오염(汚染) 토끼 비장(脾臟)의 전자현미경적(電子顯微鏡的) 관찰(觀察)

이차수,권영란,정종식,신태균,Lee, Cha-soo,Kwon, Young-ran,Jyeong, Jong-sik,Shin, Tae-kyun 대한수의학회 1993 大韓獸醫學會誌 Vol.33 No.1

An acute fatal infectious disease in rabbits has been outbroken in Korea since 1985. This disease has been characterized as an acute hepatitis caused by viruses. However, viral pathogenesis in rabbit viral hepatitis leading to sudden death remain unclear. This report dealt with the electron microscopic findings on the spleen of experimentally infected rabbits, because spleen is one of the affected organs which have high titer of virus by a haemagglutination test. A typical crystalline array of virus was not found in the splenic cells of infected rabbits with acute hepatitis. Virus-like particles were seen within the phagosome of macrophages of the spleen. Ultrastructural changes in the spleen were severe with the lapse of time after inoculation. From these results, virus-like particles in the spleen were supposed to be phagocytosed by macrophage during viremia, while active replication of virus occurred in the liver. It was concluded that sudden death in this viral disease was caused by hepatic coma and/or circulatory disturbance.

소의 이상산(異常産)에 대한 형청항체가 검사

김영환 ( Young Hwan Kim ),정종식 ( Jong Sik Jyeong ),원문부 ( Bunhu Hara ),죽하성일랑 ( Sei I ChroTake Sida ) 한국가축위생학회 1997 韓國家畜衛生學會誌 Vol.20 No.1

The cattle serum was investigated many cases of deformity calf in kyung-ju area. The serum antibody titer of Aino virus, which was not reported in domestic, was showed 3 cattle to ×2~64.