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RISS 인기검색어
- 검색키워드
- 저자 : 정일하 ( Il Ha Jeong ) (검색결과 3 건)
- 무료
- 기관 내 무료
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정일하,지상우,노성수,Il-ha Jeong,Sang-woo Ji,Seong-soo Roh 대한한방내과학회 2023 大韓韓方內科學會誌 Vol.44 No.3
Objective: Liver fibrosis is a highly conserved wound-healing response and the final common pathway of chronic inflammatory injury. This study aimed to evaluate the potential anti-fibrotic effect of the combination of Rhei Radix et Rhizoma water extract (RW) and silymarin in a thioacetamide (TAA)-induced liver fibrosis model. Methods: The liver fibrosis mouse model was established through the intraperitoneal injection of TAA (1 week 100 mg/kg, 2-3 weeks 200 mg/kg, 4-8 weeks 400 mg/kg) three times per week for eight weeks. Animal experiments were conducted in five groups; Normal, Control (TAA-induced liver fibrosis mice), Sily (silymarin 50 mg/kg), RSL (RW 50 mg/kg+silymarin 50 mg/kg), and RSH (RW 100 mg/kg+silymarin 50 mg/kg). Biochemical analyses were measured in serum, including aspartate aminotransferase (AST), alanine aminotransferase (ALT), malondialdehyde (MDA), and ammonia levels. Liver inflammatory cytokines and fibrous biomarkers were measured by Western blot analysis, and liver histopathology was evaluated through tissue staining. Results: A significant decrease in the liver function markers AST and ALT and a reduction in ammonia and total bilirubin were observed in the group treated with RSL and RSH. Measurement of reactive oxygen species and MDA revealed a significant decrease in the RSL and RSH administration group compared to the TAA induction group. The expression of extracellular matrix-related proteins, such as transforming growth factor β1, α-smooth muscle actin, and collagen type I alpha 1, was likewise significantly decreased. All drug-administered groups had increased matrix metalloproteinase-9 but a decreasing tissue inhibitor of matrix metalloproteinase-1. RSL and RSH exerted a significant upregulation of NADPH oxidase 2, p22<sup>phox</sup>, and p47<sup>phox</sup>, which are oxidative stress-related factors. Furthermore, pro-inflammatory proteins such as cyclooxygenase 2 and interleukin-1β were markedly suppressed through the inhibition of nuclear factor kappa B activation. Conclusions: The administration of RW and silymarin suppressed the NADPH oxidase factor protein level and showed a tendency to reduce inflammation-related enzymes. These results suggest that the combined administration of RW and silymarin improves acute liver injury induced by TAA.
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RAW 264.7 cell에서 생강나무 가지 추출물의 항염증 효과
이주형 ( Ju Hyung Lee ),이진아 ( Jin A Lee ),김민주 ( Min Ju Kim ),정일하 ( Il-ha Jeong ),노성수 ( Seong-soo Roh ) 한약응용학회 2022 한약응용학회지 Vol.22 No.1
Objective : This study aimed to investigate the activity according to the extraction method of Lindera obtusilba Bulme Twig (LO) in lipopolysaccharide (LPS) -induced RAW264.7 cells. Method : The methods of the two extractions included 70% ethanol and boiling in water (water extract, LOW; 70% ethanol extract, LOE). Total polyphenol, total flavonoid, EPPH and ABTS radical scavenging assays were performed to compare in vitro antioxidant effects. LOW and LOE (50, 100, and 200μg/mL) were applied to 0.1 μ g/mL LPS-induced RAW 264.7 macrophages in vitro. The level of nitric oxide (NO) and pro-inflammatory cytokines in the supernatant fraction were determined using Gress regent and the enzyme-linked immunosorbent assay (ELISA). The mitogen-activated protein kinase (МАРК) subgroups extracellular signal-regulated kinase (ERK), С-Jun N-terminal kinase (JNK), and p38 activation in extracts were detected via Western blot. Moreover, mRNA levels of cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) were measured using Real-time polymerase chain reaction (qPCR). Result : The increased NO level in the bPS-only-treated group was inhibited in a concentration―dependent manner by the treatment of two samples. Among the inflammatory cytokines, TNF-α was significantly inhibited only in the LOE (100 and 200 g/mL), but IL-1β and IL-6 were significantly inhibited in a concentration―dependent manner in all drug treatment groups. The protein expressions of MAPKs (ERK, JNK, and p―38) showed that the LOW treatment affected only p-ERK whereas the ELT treatment affected all three factors. In particular, it was confirmed that the levels of COX-2 and iNOS mRNA were more dominant in the LOE treatment than in the LOW treatment. Conclusions : Our findings indicate that the LOE treatment exerted a superior anti-inflammatory effect on inflammation induced by LPS than the LOW treatment in the occurrence of inflammation.
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薑黃 桂枝 복합물이 RAW 264.7 세포에서 항염증 활성에 미치는 영향
최지 ( Ji Choi ),박해진 ( Hae-jin Park ),정일하 ( Il-ha Jeong ),김민주 ( Min Ju Kim ),신미래 ( Mi-rae Shin ),노성수 ( Seong-soo Roh ),박순애 ( Soon-ae Park ),김미림 ( Mi-lim Kim ) 대한본초학회 2023 大韓本草學會誌 Vol.38 No.2
Objectives : A persistent inflammatory response can cause diseases such as fibrosis, cancer, and allergies. This study aimed to investigate the anti-inflammatory activity of Curcumae longae Rhizoma and Cinnamomi Ramulus Mixture (CCM) in lipopolysaccharide (LPS)-stimulated RAW264.7 cells. Methods : The total polyphenol and flavonoid contents of CCM were confirmed through an in vitro experiment. Also, radical scavenging activities of 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2'-azino-bis(3-ethylbenzothiazoline-6- sulfonic acid) (ABTS), and Hydroxyl were confirmed. Moreover, ferric reducing antioxidant power (FRAP) activity were confirmed. After, CCM (50, 100, and 200 μg/mL) were applied to 0.1 μg/mL LPS-stimulated RAW264.7 cells. The levels of nitric oxide (NO) and pro-inflammatory cytokines in the supernatant fraction were determined. Also, the expressions of mitogen-activated protein kinase (MAPK) and nuclear factor-κB (NF-κB) pathways were detected using Western blot. Results : As a result of in vitro experiments, there was an excellent antioxidant activity in CCM-treated cells. In addition, in RAW264.7 cells stimulated with LPS, the increased NO level was inhibited in a concentration-dependent manner by the treatment of CCM. In addition, inflammatory cytokines production were significantly inhibited in a concentration-dependent manner in CCM-treated group. CCM treatment significantly decreased the protein expressions of MAPKs. Moreover, the expressions of NF-κBp65 and cyclooxygenase-2 (COX-2) were significantly decreased when 200 mg/kg of CCM was applied, and phospho-inhibitor of nuclear factor kappa B-α (p-IκBα) and inducible nitric oxide synthase (iNOS) were significantly decreased at all concentrations treated with CCM. Conclusion : Our findings show that CCM exhibited excellent antioxidant activity and exhibited superior anti-inflammatory effect through the MAPKs and NF-κB pathways in LPS-stimulated RAW 264.7 macrophages.
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