전신성 홍반성 루푸스 환자에서 Total Anti-oxidant Capacity와 질병활성도의 상관성

정원태 ( Won Tae Chung ),이성원 ( Sung Won Lee ),김경희 ( Kyung Hi Kim ),김정만 ( Jung Man Kim ),김채기 ( Chae Gi Kim ),윤원찬 ( Wern Chan Yoon ),류재근 ( Jae Kean Ryu ),장성국 ( Sung Gug Chang ),송용호 ( Yong Ho Song ),최정윤 ( J 대한류마티스학회 2001 대한류마티스학회지 Vol.8 No.3

Objective: To determine the correlation between total anti-oxidant capacity (TAOC) and lipid peroxidase (LPO) levels in serum and disease activity in systemic lupus erythematosus (SLE). Methods: The study population consisted of 99 patients with SLE according to the 1982 revised ACR criteria and 83 healthy controls. The serum TAOC levels in 99 patients with SLE and 83 healthy controls by the ABTS(R) inhibition method (Randox Ltd, Antrim, UK) and serum malondialdehyde (MDA) levels in 37 out of 99 patients with SLE were measured. Anti-dsDNA antibody (anti-dsDNA), albumin, AST, ALT, cholesterol, uric acid and creatinine were measured to determine the association with the serum TAOC levels. The correlation between the serum TAOC levels and nephritis in 27 cases with nephritis out of 99 SLE patients was also investigated. The SLE disease activity was determined by the SLE disease activity index (SLEDAI) at the time of sample collection. Results: Compared to the controls (1.37±0.127mmol/L), serum TAOC levels were significantly decreased (1.29±0.124mmol/L) in SLE patients (p=0.001). And there was a negative correlation between serum TAOC levels and SLEDAI total scores in the SLE patients (r=-0.388, p=0.0001), but no significant correlation between TAOC levels and C3 and anti-dsDNA. Further, a significant difference (p<0.04) in TAOC levels was found in SLE patients with and without nephritis. In comparison with other parameters such as AST, ALT, and cholesterol which might change anti-oxidant level, there was no correlation between the serum TAOC levels and them, except for serum uric acid (r=0.387, p=0.0001), creatinine, and albumin (r=0.507, p=0.0001). Additionally, we couldn`t find significant correlation between the serum TAOC levels and MDA. Conclusions: The serum TAOC levels correlate significantly with SLE disease activity and are associated with nephritis. Also, this study showed a significant correlation with serum albumin levels in these patients. Conclusively, the measurement of the serum TAOC levels in patients with SLE will provide useful information on SLE disease activity.

한국인 정상성인의 지질분포에 관한 연구

정원태(Won Tae Chung),신영기(Yeong Kee Shin),배상균(Sang Kyun Bae),이민기(Min Ki Lee),이동일(Dong Il Lee),문창형(Chang Hyung Moon) 대한내과학회 1994 대한내과학회지 Vol.47 No.5

N/A Objectives: The study was designed to establish normal serum lipid levels in the Korean adult population and to identify the effect of sex, age, blood pressure, body mass index (BMI), smoking, alcohol consumption, exercise, economic status, occupation and location on the serum lipid levels. Methods: The serum total cholesterol and triglyceride distribution in the normal Korean adult population were studied in 5,342 cases (males 2,834 and females 2,508) in the age groups of 3rd to 8th decades. Data were collected from 33 institutes and hospitals throughout the country during the period of June 1989 to December 1990. The serum lipid level were examined in the state of fasting of at least 12 hours and by the enzymatic method, Results: The mean level of serum total cholesterol was 183.6 mg/dl. Males had 181.9 mg/dl and females had 185.5 mg/dl without statistical significance between gender, but levels were significantly higher in the 4th decade in males and in the 6th, 7th and 8th decades in females. The mean level of serum triglyceride was 131.9 mg/dl. Males had 140.5 mg/dl and females had 122.2 mg/dl without statistical significance between gender, but levels were significantly higher in the 4th and 5th decades in males and in the 7th decade in females. The lipid levels rose gradually with the increase of age. The peak of serum total cholesterol and triglyceride level for the males was in the 6th decade and that for the females was in the 7th decade. The incidence of hypercholesterolemia with the standard of 240 mg/dl or more was 8.7% Males had 7.2% and females had 10.4% with the significantly higher incidence in females. The incidence of more than 220 mg/dl was 17.5% Males had 15.1% and females had 19.2% with higher incidence in females having statisticai significance. But there was no significant difference between big cities (cities controlled by central government) and smaller cities & towns in the incidence with either standard of levels. The incidence of hypertriglyceridemia of more than 200 mg/dl was 15%. Males had 18.0% and females had 11.5% with the significantly higher incidence in males. The incidence of more than 150 mg/dl was 29.1%. Males had 33.2% and females had 24.3% with higher incidence in males being significance. There was no significant difference between big cities and smaller cities & towns, The incidence of low serum total cholesterol less than 140 mg/dl was 12.4%, Males had 13.0%, and females had 11.7% without significant difference between gender. The incidence of less than 120 mg/dl was 3.7%. Males had 4.2% and females had 3.2%. It was significantly higher in males, and in the comparison between big cities and smaller cities & towns the incidence was significantly higher in smaller cities k towns compared with big cities in the standard of 140 mg/dl but not in the standard of 120 mg/dl. The significantly related factors for the serum total cholesterol level were age, sex, BMI, systolic blood pressure, occupation, location and family history. For the triglyceride level age, sex, BMI, diastolic blood pressure and smoking were the significantly related factors. Conclusion: The results of this research indicates that present lipid level profile in the adult Koreans is not a serious health situation. Additional research and on- going studies should be made to update this study. In addition an educational campaign to inform the medical profession and citizens of the danger of this health problem should be undertaken to avoid the health problems faced by other advanced countries.

류마티스 관절염 환자의 슬관절 활막염에서 T 세포 수용체 레퍼토리의 시차에 따른 비교 연구

정원태 ( Won Tae Chung ),김진호 ( Jin Ho Kim ),장광열 ( Kwang Yul Chang ),이성욱 ( Sung Wook Lee ),김종성 ( Jong Seong Kim ),박순규 ( Soon Kew Park ),정성수 ( Sung Soo Jung ),김성윤 ( Seong Yoon Kim ) 대한류마티스학회 1997 대한류마티스학회지 Vol.4 No.1

류마티스 관절염(Rheumatoid Arthritis; RA)의 면역학적 병인에 있어 T 림프구가 중추적인 역할을 하는 것은 잘 알려져 있다. 최근에 관절염 병인성 T 림프구의 특성을 규명하고자 하는 시도가 여러 연구자들에 의해서 진행되고 있다. 이에 저자들은 관절염 병인성 T 림프구의 특성을 규명하는 방법으로서 류마티스 관절염 환자에서 발병 시기가 서로 다른 두 관절로부터 활액을 얻어 T 세포수용체(TCR)의 Vβ 유전자 사용 빈도를 검색하여 비교함으로써 공통적으로 우세한 Vβ 유전자계를 찾는 데 목적을 두었다. 방법: 증상 시작이 7개월 된 류마티스 관절염 환자로, 양측 손목과 중수지 관절에서 관절염이 시작되어 활액과 말초 혈액의 시료를 얻기 2주 전에 우측 슬관절에, 1주 전에 좌측 슬관절에 부종을 경험한 환자로부터 양측 슬관절에서 활액을 얻어 T 림프구를 분리한 다음 이 T 림프구로부터 추출한 RNA를 이용하여 cDNA를 합성한 뒤 이 cDNA를 주형으로 Vβ family specific oligonucleotide를 시발체(primer)로 반 정량적 역전사 연쇄중합반응(semiquantitative RT-PCR)을 시행하여 T 세포수용체(T cell receptor; TCR) Vβ 레퍼토리를 비교 분석하여 다음과 같은 결과를 얻었다. 결과: 1. CD4+ T 세포 아형의 T 세포 수용체 Vβ 유전자 레퍼토리 사용 빈도 1) 활액과 말초 혈액 비교; 활액에서 말초 혈액보다 2배 이상의 사용 빈도를 보인 CD4+ T 세포 아형의 Vβ계는 Vβ14(우측 슬관절: 3.83%, 좌측 슬관절: 3.39%) 유전자계였다. 2) 우측과 좌측 슬관절 비교; 우측 부위에서 좌측에 비해 우세하게 많은 빈도로 사용된 Vβ계는 Vβ15(Rt: 2.98%, Lt: 1.15%), Vβ18(Rt: 3.92%, Lt: 2.25%) 유전자계였다. 반대로 좌측 부위에서 우측보다 우세하게 많은 빈도로 사용된 Vβ계는 Vβ1(Rt: 2.17%, Lt: 4.78%), Vβ2(Rt: 0.63%, Lt: 5.84%), Vβ5.1(Rt: 1.07%, Lt: 3.39%), Vβ5.2(Rt: 0.31%, Lt: 1.91%), Vβ8(Rt: 0.19%, Lt: 4.20%), Vβ9(Rt: 0.12%, Lt: 2.61%), Vβ10(Rt: 1.27%, Lt: 4.08%), Vβ12(Rt: 0.99%, Lt: 4.53%), Vβ22(Rt: 0.38%, Lt: 3.85%), Vβ23(Rt: 0.63%, Lt: 3.99%) 유전자계였다. 2. CD8+ T 세포 아형의 T 세포 수용체 Vβ 유전자 레퍼토리 사용 빈도 1) 활액과 말초 혈액 비교; 활액에서 말초 혈액보다 2배 이상의 빈도를 보인 Vβ계는 Vβ1(Rt: 5.03%, Lt: 2.17%), Vβ5.2(Rt: 2.32%, Lt: 0.56%), Vβ9(Rt: 1.95%, Lt: 0.32%), Vβ10(Rt: 3.70%, Lt: 0.55%), Vβ13(Rt: 2.57%, Lt: 1.90%) 유전자계였다. 2) 우측과 좌측 슬관절 비교; 우측 부위에서 좌측에 비해 2배 이상 많은 빈도로 사용된 유전자계는 Vβ1(Rt: 5.03%, Lt: 2.17%), Vβ5.2(Rt: 2.32%, Lt: 0.56%), Vβ8(Rt: 3.62%, Lt: 1.55%), Vβ(Rt: 1.95%, Lt: 0.32%), Vβ10(Rt: 3.70%, Lt: 0.55%), Vβ15(Rt: 1.44%, Lt: 0.77%), Vβ(Rt: 4.31%, Lt: 1.38%), Vβ17(Rt: 2.74%, Lt: 0.44%), Vβ19(Rt: 3.29%, Lt: 1.64%), Vβ21(Rt: 4.11%, Lt: 0.52%), Vβ22(Rt: 5.64%, Lt: 1.19%), Vβ23(Rt: 1.86%, Lt: 0.30%)계였다. 반대로 좌측부위에서 우세하게 사용된 유전자계는 없었다. 결론: 초기 관절염에서 CD4+ T 림프구가 주도적인 역할을 하지만, 본 연구에서 특정 Vβ 유전자의 편향은 관찰할 수 없었다. 향후 CDR3 부위의 염기서열을 확인하는 방법을 사용하여 자가 면역성이 있는 T 림프구 클론의 검색을 시행해야 할 것으로 생각된다. Objective: To understand T cell role in the immunopathogenesis of rheumatoid arthritis (RA), authors investigated Vβ usage of T cell receptor (TCR) in different onset RA lesion of the same patient using a reverse transcriptase-polymerase chain reaction. The current pathogenic model of RA plays a critical role in CD4+ T cells, which are thought to be able to recognize a disease-relevant antigen in the joint. In this model, activation of a certain, specific, antigen induced T cells plays a pivotal role in the development and maintenance of chronic inflammation. To search a common clone in different onset of inflammatory joints will furnish the most exact information about T cells which playa role at initiation and perpetuation of synovitis. Here, We first characterized the change in TCR Vβ shape with elapsing time between the two joints that have the consecutive inflammation. Methods: Synovial fluids and peripheral blood were obtained from a patient with active RA who had two successively developing inflammatory joints with 1 week interval(the right knee joint(RT) was involved first and the left knee joint (LT) later). RT-PCR technology was employed to examine synovial fluid and peripheral T cells. Oligonucleotide primers specific for individual TCR Vβ gene families were used to amplify the TCR gene products in a semiquantitative assay of their relative utilization in fresh T cells subpopulations(CD4+, CD8+ T cells). Results: The CD4+ T cells with TCR Vβ l(LT: 4.78%, RT: 2.17%), Vβ 2(LT: 5.84%, RT: 0.63%), Vβ 5.l(LT: 3.40%, RT: 1.07%), Vβ 5.2(LT: 1.91%. RT: 0.31%), Vβ 8(LT: 4.20%, RT: 0.19%), Vβ 9(LT: 2.61%, RT: 0.12%), Vβ 10(LT: 4.08%, RT: 1.27%), VP 12(LT: 4.53%, RT: 0.99%), VP 22(LT: 3.85%, RT: 0.38%), Vβ 23 (LT: 3.99%, RT: 0.63%) were used more predominantly in the left knee joint than in the right knee joint while VP 15 and 18 were used far more in the right knee joint. The CD8+ T cells were used less frequently in the left side than in the right side except the Vβ 3, 4 and 7 families. Conclusions: Among the CD4+ T cells. TCR Vβ 1, 2, 5.1, 5.2, 8, 9, 10, 12, 22, 23 families might play a key role in early symptomatic synovitis in RA. The role of TCR Vβ 15 and 18 families increase in progressing synovitis with time. On the other hand, the late recruitment of CD8+ T cells in the inflamed site might be related to nonspecific inflammatory reaction.

음용수의 염소처리 시 Ca<SUP>2+</SUP>, Mg<SUP>2+</SUP>의 첨가에 따른 Trihalomethane(THM)과 Haloacetic acids(HAA) 형성에 대한 영향

정원태(Won-Tae Jeong),신다희(Da Hee Sin),김덕현(Deok Hyeon Kim),정근욱(Keun-Yook Chung) 한국토양비료학회 2013 한국토양비료학회 학술발표회 초록집 Vol.2013 No.10

철근 콘크리트 깊은보의 전단 거동에 대한 개구부의 영향

정원태(Won-Tae Jung),양근혁(Keun Hyeok Yang),이영호(Young-Ho Lee),은희창(Hee-Chang Eun),정헌수(Heon-Soo Chung) 한국콘크리트학회 2002 한국콘크리트학회 학술대회 논문집 Vol.2002 No.11

류마티스 관절염에 동반된 이차성 아밀로이드증의 임상양상

이성원 ( Sung Won Lee ),이종훈 ( Jong Hoon Lee ),김경희 ( Kyeong Hee Kim ),정원태 ( Won Tae Chung ) 대한류마티스학회 2004 대한류마티스학회지 Vol.11 No.4

Objective: In order to study the clinical manifestations in 17 cases of secondary amyloidosis with rheumatoid arthritis (RA). Methods: We investigated the clinical characteristics and survival of 17 RA (8 men and 9 women) patients with biopsy-proven secondary amyloidosis who were followed up in our department from 1998 to 2004 by medical records review. Results: The mean age and duration of RA at diagnosis of amyloidosis were 58.4 (range 35∼81) years and 7.8 (range 2∼33) years respectively. Upper gastrointestinal tract biopsy was performed in 14, rectal and abdominal fat biopsy in 4 and 2, and renal and bladder biopsy in 3 and 2 patients respectively. Sixteen patients (94.1%) were diagnosed as secondary amyloidosis by endoscopic biopsy. Common symptoms were diarrhea and abdominal pain. Serological activities determined by C-reactive protein (CRP) and erythrocyte sedimentation rates (ESR) were moderate to high in the patients. Three patients died 2 to 45 (mean 19.3) months after the diagnosis of amyloidosis. One patient died of sepsis after etanercept therapy for treatment of RA, the other of intractable diarrhea, and another of acute myocardial infarction. Conclusion: Our results indicate that the mainly involved organ in secondary amyloidosis associated with RA is gastrointestinal tract rather than kidney and the biopsy in the second portion of duodenum may be a good diagnostic tool for the evaluation of secondary amyloidosis.

전신성 홍반성 루푸스 환자에서 항리보솜항체 (Antiribosomal-P Antibody)의 발현빈도와 임상양상에 대한 고찰

이성원 ( Sung Won Lee ),정원태 ( Won Tae Chung ) 대한류마티스학회 2000 대한류마티스학회지 Vol.7 No.3

Objective: To determine the frequencies and clinical associations of anti-ribosomal P antibodies (anti-P) in patients with systemic lupus erythematosus (SLE). Methods: Sera from 99 patients with SLE were analyzed for anti-P using an enzyme-linked immunosorbent assay (ELISA) and clinical symptoms, signs and SLE disease activity index (SLEDAI) in all patients were evaluated by retrospective clinical chart review. Results: The frequency of anti-P was 24 of 99 SLE patients (24.2%). Of these patients, three patients were male (12.5%) and 21 patients were female (87.5%). The correlation coefficient between anti-P and anti-ds-DNA antibody (anti-ds-DNA) was 0.205 (p=0.042) and between C3 and anti-P was -0.235 (p=0.024). Of 24 patients with anti-P, none had lupus psychosis. The other clinical features of the patients examined in relation to anti-P status were nephritis, hematologic abnormalities, arthritis, skin lesions, CNS lesions, acute myoacardial infarction, and autoimmune hepatitis but there was no significant association with anti-P. Some patients with anti-P had concomitant ENA, 7 had anti-Ro antibody (anti-Ro), 2 had anti-La antibody (anti-La) and 10 had anti-RNP antibody (anti-RNP) that was the most frequently observed ENA, but there was no significant association between anti-P and ENA. ANA types in patients with anti-P were cytoplasmic pattern in three patients, homogenous in two, peripheral in three, and speckled type in 14 patients. The most frequent ANA type in SLE patients with anti-P was speckled type but statistically significant difference could not shown. Conclusion: The frequency of anti-ribosomal P antibody was 24.2%. Anti-ds-DNA had weak positive correlation (p=0.042) and C3 had weak negative correlation (p=0.024) with anti-P. In contrast to the findings of some previous studies, an increased frequency of lupus psychosis, nephritis, and hepatitis was not observed and there was no significant correlation between lupus psychosis, nephritis, hepatitis, SLEDAI and anti-P in this study.

환경기초시설에 대한 시민 의식 조사

정재춘(Jae-Chun Chung),정원태(Won-Tae Chung),탁성제(Seung-Je Tak),강헌(Hun Kang) 유기성자원학회 1995 유기물자원화 Vol.3 No.2

환경기초시설에 대한 시민의 의식을 알아보기 위하여 설문조사를 실시하였다. 대부분의 시민이 쓰레기 처리문제의 심각성을 인식하고 있었으며 퇴비화시설을 가장 많이 지어야 한다고 느끼고 있었다. 시민들이 가장 싫어하는 시설은 매립장이었고 분뇨처리장, 소각장, 퇴비화시설의 순이었다. 집과의 허용이격거리는 매립장은 4km 이상 소각장과 퇴비화시설은 2km 이상 하수처리장은 1km 이상 떨어져야 한다고 응답한 사람들이 많았다. 지역주민이 원하는 보상정도는 이사비용 + 토지가 + 불편감수비용 (토지 및 건물가격과 비퉁한 금액) 을 원하는 사람이 가장 많았다. 처리시설에 대한 수용도에 있어서는 완벽한 처리시설이라도 30% 정도의 응답자가 허용을 하지 않겠다고 응답하였다. A questionare survey was performed to investígate the citizen's attitude concerning waste treatment facílíties. Most people recognized the seriousness of the waste treatment problem and think that more composting facilities should be built. People dislíke most the landfill facility, followed by the nightsoíl treatment facílity, the íncíneration facílíty and the compostíng facility. Most people answered that the disirable distance from their house to the treatment facílity should be farther than 4km for the landfill facílity, farther than 2km for incineration and composting facílities and farther than lkm for the wastewater treatment plant. Most people want moving cost + land price+inconvience suffering cost (amount equal to the land prize+building prize) for their retrieval. About 30% of people answered that they will not accept any waste treatment facility even though it is perfect.

유리용기의 재활용 흥진률 위한 제도 개선 방안

정재춘(Jae-Chun Chung),정원태(Won-Tae Chung) 유기성자원학회 1996 유기물자원화 Vol.4 No.1

최근들어 유리병 재활용에 대한 관심이 고조되고 있다. 이것은 자원의 절약과 쓰레기 감량화의 관점에서 볼때 유리병 재활용의 중요성이 점점 더 크게 부각되고 있기 때문이라 생각된다. 본 논문에서는 국내 유리병 회수 · 재활용 체계의 현황과 문제점을 분석해 보고 외국의 재활용 현황을 요약하였다. 또한 시민들의 의식조사에 기초하여 유리병의 재활용 증진을 위한 법적, 제도적 개선방안을 제시하였다. Recently, signigicant interest is given to the recycling of glass bottles. This is because the importance of glass bottle recycling is growing in terms of resource conservation and waste minimization. In this paper, authors analyzed the present situation and problems in glass bottle recycling, summarized the recycling activity in foreign countries and suggested legal and institutional strategies based upon citizen’s attitude survey to improve glass bottle recycling activIty

SIADH(항이뇨호르몬 부적절분비증)를 동반한 세균성 폐렴

박현 ( Hyun Park ),정원태 ( Won Tae Chung ),이민기 ( Min Ki Lee ),강영진 ( Yeong Jin Kang ),박순규 ( Soon Kew Park ),신영기 ( Yeong Kee Shin ) 대한결핵 및 호흡기학회 1988 Tuberculosis and Respiratory Diseases Vol.35 No.2