http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
2006~2007년 국내 종오리 농장에서의 HPAI 증례 보고
김민정 ( Min Jeong Kim ),조성준 ( Seong Joon Joh ),김민철 ( Min Chul Kim ),이윤정 ( Youn Jeong Lee ),정옥미 ( Ok Mi Jeong ),우계형 ( Gye Hyeong Woo ),권준헌 ( Jun Hun Kwon ),모인필 ( In Pil Mo ) 한국예방수의학회(구 한국수의공중보건학회) 2011 예방수의학회지 Vol.35 No.2
Korea had experienced second epidemic highly pathogenic avian influenza (HPAI), and there were the seven affected farms, including two breeder duck farms, from 22nd November 2006 to 6th March 2007. Here, we reported the clinico-pathological characteristics of domestic breeder ducks farms naturally infected with HPAI virus (H5N1). Clinically, the most ducks showed various signs from depression, decreased egg production and feed consumption to even, death. The most commonly gross changes were hepatomegaly, splenomegaly, petechial and ecchymotic hemorrhage on the liver surface, a white stripe on the cardiac muscle, and severely hemorrhagic and deformed eggs. The most significant histopathological changes were necrosis of various cells such as neuron, lymphocytes, cardiac myocytes, hepatocytes, blood vessels and pancreatic acinar epithelium. The viral antigen was mainly detected in the endothelium of blood vessels of various organs and tissues, peripheral nerves and neuronal cells. Based on the above results, we identified that HPAI H5N1 induced systemic infection in the adult breeder ducks.
채현영(HyunYoung Chae),정옥미(Ok-Mi Jeong),허문(Moon Her),강정우(JeongWoo Kang) 한국예방수의학회 2021 예방수의학회지 Vol.45 No.4
The objective of this study was to develop a simultaneous method for 8 amino acids including alanine, arginine, glutathione, lysine, ornithine, methionine, threonine and tryptophan in veterinary products using LC-MS/MS. To optimize MS analytical condition of 8 amino acids, each parameter was established by multiple reaction monitoring in positive mode. The chromatogram separation was achieved on a C18 column with mobile phase of 0.1% formic acid in D.W. and 0.1% formic acid in acetonitrile for green technology at a flow rate of 0.4mL/min for 5 min with gradient elution. The developed method was validated for mass accuracy, precision, linearity in veterinary products. Calibration curves were linear over the calibration ranges (0.5 - 10 mg/L) for all the analytes r2>0.99. Average recoveries were 92.96 - 105.61% and relative standard deviations (RSD) were 0.27 - 3.5%. The limit of detection (LOD) and the limit of quantification (LOQ) were 0.04 - 0.83 mg/L and 0.12 - 2.52 mg/L, respectively. All values were corresponded with the criteria ranges requested by CD 2002/657/EC. The application of this method will be helpful in quality control analysis of amino acids in veterinary products.