감잎차 제조를 위한 감잎의 성장시기별 함유 성분의 변화

정선화(Sun-Hwa Chung),문광덕(Kwang-Deok Moon),김종국(Jong-Kuk Kim),성종환(Jong-Hwan Seong),손태화(Tae-Hwa Sohn) 한국식품과학회 1994 한국식품과학회지 Vol.26 No.2

메나디온에 의한 혈소판 내 칼슘 변화측정시 형광 색소 사용의 문제점

정선화(Sun Hwa Chung),이무열(Moo Yeol Lee),이주영(Joo Young Lee),정승민(Seung Min Chung),정진호(Jin Ho Chung) 대한약학회 1997 약학회지 Vol.41 No.6

It has been reported that dose-dependent Ca2+ increase by menadione in platelets could be measured by fluorescent dye, quin-2. The problems will be described here relating to measuring Ca2+ in menadione-exposed platelets using fura-2 and fluo-3, widely used fluorescent indicators. Additions of menadione to fura-2 loaded platelets and their lysates resulted in marked reduction in fluorescence intensity at both 340nm (Ca2+-unbound form) excitation wavelengths. Fura-2 excitation spectra were overlapped with UV-visible absorption spectra of menadione, suggesting that light absorption by menadione itself could quench fluorescence generated by fura-2. Next approach was to use fluo-3 which has the higher wavelength (490nm) of excitation. Previous work demonstrated that treatment with probenecid to platelets was required to prevent fluo-3 dye leakage. However, probenecid itself was proven to be inadequate to measure the concentration of intracellular Ca2+ by reducing menadione-induced cytotoxicity in platelets. Our results suggest that it is not feasible to measure Ca2+ in platelets by using fura-2 and fluo-3 in the presence of probenecid, and cautions should be taken to measure changes of intracellular Ca2+ levels by fluorescent dyes following chemical exposure.

비압착 냉동저장 두부의 조직감 변화에 미치는 항냉동제의 효과

정선화(Sun-Hwa Chung),최원석(Won-Seok Choi),손혜숙(Hye-Sook Son),이철호(Cherl-Ho Lee) 한국식품과학회 1999 한국식품과학회지 Vol.31 No.4

Menadione의 대사체인 Menadione-Glutathione Conjugate(MEN-SG)가 흰쥐 혈소판에 미치는 세포독성의 평가 및 MEN-SG의 안정성에 관한 연구

서동철,정선화,이주영,김미정,정진호,Seo, Dong-Chul,Chung, Sun-Hwa,Lee, Joo-Young,Kim, Mee-Jeong,Chung, Jin-Ho 한국독성학회 1995 Toxicological Research Vol.11 No.2

Menadione-ghitathione conjugate (MEN-SG), a metabolite of menadione, is known to be a redoxcycler in rat hepatocyte subcellular fraction. Therefore, it was assumed that MEN-SG could exert cytotoxlclty to ral platelets, another target tissue of menadione. We first synthesized MEN-SG, the identity of which was verified by mass, $^1{H}$-NMR and UV-visible spectra. In addition, the stability of MEN-SG was investigated in biological assay system. MEN-SG was degraded in a time-dependent manner in DMSO which had been used as a vehicle and thus, tris-HCl buffer was used as a vehicle of MEN-SG despite the low solubility in it. Perchloric acid as well as platelets itself did not affect the stability of MEN-SG. Our next attempt was the evaluation of cytotoxicity of MEN-SG in rat platelets. MEN-SG did not induce cytotoxicity to rat platelets measured by two different methods, LDH release and turbidity changes. The extents of oxygen consumption by MEN-SG in intact platelets were significantly lower than those by menadione, though it had been observed that oxygen consumptions by menadione and MENSG were similar in subcellular fractioas of platelets. These results suggest that MEN-SG is not toxic to rat platelets despite its redox cycling capacity and glutathione conjugation reaction of menadione could be regarded as a detoxification process.

hESC로부터 유래된 혈관내피전구세포를 위한 최적의 배양조건 확립

김주미 ( Ju Mi Kim ),문성환 ( Sung Hwan Moon ),이민지 ( Min Ji Lee ),오인록 ( In Rok Oh ),신정민 ( Jeong Min Shin ),박순정 ( Soon Jung Park ),정선화 ( Sun Hwa Chung ),김문규 ( Mun Kyou Kim ),이경일 ( Kyung Il Lee ),정형민 ( Hyung 한국조직공학과 재생의학회 2008 조직공학과 재생의학 Vol.5 No.3

Human embryonic stem cell(hESC) can be very valuable source for the cell therapy due to their pluripotency when they differentiated into specialized cell types. Conventionally, differentiated cell population were obtained from spontaneously formed embryoid body(EB) which could contain various cell types. In this study, we described the optimization of culture condition for endothelial precursor cells derived from human embryonic stem cells(hESC-EPCs). First, we compared the expression of endothelial precursor cell markers such as CD133, CD34 and KDR in day 7 hEBs and isolated CD133 and KDR double positive population as hESC-EPCs. Second, we compared the culture medium and ECM coating to optimize culture condition for hESC-EPCs. As the result, we could confirm EGM-2 and collagen coating were optimized condition to culture of hESC-EPCs. After conducting optimized culture condition for hESC-EPCs, we examined the maintenance of characteristics of hESC-EPCs cultured in optimized culture condition as vascular cells. Finally, we could confirm the characteristics of hESC-EPCs cultured in optimized condition as vascular cells were maintained in developed optimized culture condition. Furthermore, the optimized culture condition developed in this study will be useful to culture of other vascular lineage cells derived from hESCs.

인간배아줄기세포 유래 배상체내의 신경세포군 확인과 분화과정 동안 이뤄지는 신경발생과정의 이해

김동환 ( Dong Hwan Kim ),문성환 ( Sung Hwan Moon ),김주미 ( Ju Mi Kim ),이민지 ( Min Ji Lee ),신정민 ( Jeong Min Shin ),이경일 ( Kyung Il Lee ),박순정 ( Soon Jung Park ),정선화 ( Sun Hwa Chung ),김희정 ( Hee Jung Kim ),정형민 ( Hy 한국조직공학·재생의학회 2009 조직공학과 재생의학 Vol.6 No.4

Recently, human embryonic stem cells(hESCs) have been highlighted to understand early embryogenesis as well as to apply to clinical cell therapy. During human embryogenesis, dynamic processes of early neuronal development are very important to elucidate their developing mechanism. However, the mechanism has not been entirely clear. The formation of embryoid bodies(EBs) derived from hESCs is a tissue-like spheroids in suspension culture and has a potential to differentiate into the three germ layers. Even though many kinds of specific cell makers have been studied, the specific proteins expressed from differentiated cells have been elusive. The objective of this study is to investigate the expression of neuronal protein while EBs is differentiating from hESCs(day 5, 10, 15 and 20). The expressions of neuronal specific makers such as Neurofilament 68kDa(NF 68), tubulin III(TuJ-1) and microtubule-associated protein 2(MAP2) in each EBs stage were analyzed by immunofluorescence(IF). The expressions and localization of neuronal specific markers were shown to be different depending on EBs stages. NF 68 positive cells were expressed on the surface of EBs at day 10 and the expression of NF68 almost disappeared at day 20. MAP2 was not detected in EBs at day 10, whereas it began to be slightly detected on the surface of EBs at day 15. On the other hand, TuJ-1 positive cells were found at all stages and their expression levels were maintained until day 20. These results demonstrate neuronal specific markers can be expressed differently depending on EBs stages, suggesting this allows us to probably understand the early development of neurogenesis in hESCs. Furthermore, this idea expands that we are able to understand the development of all specific cells differentiated from early human embryogenesis.

2003년 국내 중증급성호흡기증후군 진료 현황 및 문제점 분석

이진수,김은실,정문현,백제중,정선화,안주희,최영화,이선희,고철우,김성범,김민자,박승철,기현균,송재훈,최상호,김양수,이상오,조용균,박영훈,정숙인,김연숙,이흥범,손창희,장성희,정희진,김우주 대한감염학회 2004 감염과 화학요법 Vol.36 No.3

목적 : 2002년 말 중국에서 SARS가 발생한 이후 국내에서도 2003년 10월까지 총 3명의 추정환자, 17명의 의심환자가 보고되었다. 향후 추가적인 SARS의 유행이 우려되는 상황에서, 그간의 SARS 환자 진료에 있어서의 실질적인 준비사항, 진료 현황 등에 대한 조사를 통해 문제점을 파악하여, 향후 더 나은 대비가 될 수 있도록 개선점을 제시하고자 하였다. 재료 및 방법 : SARS로 의심되는 환자를 진료 경험이 있는 병원의료진을 대상으로 2003년 10월에 설문조사를 실시하였다. 설문에는 SARS 환자 진료 시의 실질적인 조치, 진료 현황, 병실, 응급실 및 외래에서의 격리 시설과 준비사항, 보건당국의 관리와 지원에 관한 사항을 포함하였다. 결과 : 대상이 되는 22개 병원 중 17개(17/22, 77.2%) 병원이 설문에 응하였다. SARS 환자를 위한 격리실은 응급실, 외래, 일반병실 및 중환자실에서 각각 9개(9/17, 52.9%), 5개(5/17, 29.4%), 15개(15/16, 93.7%), 4개(4/16, 25%) 병원에서 음압처리가 되어있지 않은 일인실 혹은 다인실이 사용되었고, 1개(1/16, 6.3%) 병원에서만 일반병실에서 음압격리실이 운영되었다. 입원환자의 진찰 시 개인보호구의 착용은 거의 모든 의료기관에서 이루어졌다. 보건당국에서 SARS지정병원의 시설 등을 사전에 확인한 곳은 1곳(1/12, 8.3%)이였고, 14개 병원(14/15, 93.3%)에서는 보건당국에 의뢰한 검사결과를 통하 받지 못하였다. 결론 : 의료기관에서 SARS 환자용 격리실뿐만 아니라 기존의 격리실 설비 등이 미흡하였으며, 특히 중환자실 및 외래의 준비가 더욱 부족하였다. 보건당국의 의료기관에 대한 종합적인 지원이 부족하였고, 병원과의 원활한 연계가 잘 이루어지지 않았다. SARS 만이 아닌 격리를 필요로 하는 질환의 적절한 진료를 위해 향후 병원 시설의 정비와 정부차원에서의 보다 구체적이고 실질적인 대책마련이 필요하다. Background : There was an worldwide outbreak of the Severe Acute Respiratory Syndrome (SARS) originated from China in late 2002. During that period three cases of suspected SARS and 17 cases of probable SARS were reported in Korea. With the concerns about the reemergence of SARS-coV transmission, it is important to be prepared for any possibility. So, this study is aimed to analysis the past measures in managing SARS and propose the amendatory plans to improve the preparedness. Materials & Methods : Questionnaires were collected among clinicians with any experience in managing the probable or suspected SARS cases in Oct. 2003. 17 out of 22 hospitals responded to the questionnaire. The contents in the questionnaire were practical activities, personal equipments, response plans, isolation facilities in emergency centers, outpatient clinics, general wards and intensive care units, and relationship with the public health department. Results : The dedicated isolation rooms in emergency centers, outpatient clinics, general wards, and intensive care units were prepared in 9 (9/17, 52.9%), 5 (5/17, 29.4%), 15 (15/16, 93.7%), and 4 (4/16, 25.0%) hospitals, respectively. Except for one hospital that newly made negative pressure room for SARS, single or multi-bed rooms without airborne infection control were used in all the other hospitals. The personal precaution principles were kept quite well in general wards. Before the designation of SARS hospital by the public health department prior evalution to see if the hospital was suitable for managing SARS was conducted in only 1 (1/12, 8.3%) hospital. The results of laboratory diagnosis were reported back in 1 (1/15, 6.6%) hospital. Conclusions : The isolation facilities which can control airborne infection were almost deficient not only for SARS but also for other respiratory transmissible diseases. For the infection control of transmissible diseases including SARS, more investment is needed on medical facilities and comprehensive support from the public health department required.

상대적 우위 시스템의 개발 방법에 대한 소재 및 현장적용

황선문,정선화 전주대학교 공학연구소 1997 전주대학교 공학기술종합연구소 학술논문집 Vol.3 No.3

This study has been devoted to develop a designing method for the relative advanced system which should have higher productivity than its original system. By this research, Design Method for Relatively Advanced System (DMRAS) has been developed and applied in a real system. DMRAS includes diagnosing a present system, designing alternative systems and evaluating them relatively and quantitatively, For these process, a computer simulation method was used as a designing and comparing tool for the systems. Usefulness of DMRAS was examined by its application on a medium sized assembly manufacturing company. The results from it proved that overall productivity of the alternative model was much higher than the system Since the alternative system used the same facilities as the system, the effective system could be designed by using DMRAS.

Initial Risk Assessment of Acetanilide with Respect to Human Health

Lee, Su-Rae,Choi, Seon-Ju,Lee, Mi-Kyung,Nam, U-Kyung,Chung, Sun-Hwa,Seog, Geum-Su,Park, Kwang-Sik,Kim, Kyun,Kim, Yong-Hwa 한국환경독성학회 2000 환경독성보건학회지 Vol.15 No.1

아세트아닐리드는 그의 생산 및 이용 공장에서 환경으로 방출된 다음 인체에 노출될 수 있다. 아세트 아닐리드는 진통효과를 나타내는 것으로 알려져 과도 노출시에는 건강에 부작용을 초래할 수 있다. EUSES시스템에 의하면 아세트아닐리드는 지역노출의 경우 6×10⁴을 초과하는 높은 MOS 값(안전성 마진)을 보여주어 공중보건상 충분히 안전한 것으로 나타났다. 국지수준(작업장)에서 경피노출에 의한 MOS 최저 값은 3×10⁴으로 추정되었지만 작업장에서 개인장비나 환기와 같은 예방조치에 의하여 그 위험을 부분적으로 경감시킬 수 있다. 아세트아닐리드는 분진 흡입하는 작업자에게 위험 가능성이 나타날 수 있다. 작업장에서 건강보호를 위해서는 산업보건 측면에서의 안전함이 증명될 수 있도록 반복투여독성, 생식독성 및 발육독성에 관한 자료가 보완되어야 할 것이며, 따라서 이에 대한 실험이 진행되어야 할 것으로 사료된다. Acetanilide may be released into the environment through air and wastewater from its production and use sites and exposed to human. Acetanilide is known to produce an analgesic effect and may pose adverse effects on human health by overly exposure. According to the EUSES system, acetanilide showed a high MOS (Margin of safety) value exceeding 6 × 10⁴ on a regional exposure, which is safe enough for public health. Whereas the lowest MOS value in dermal exposure was estimated as 3 x 10^(-4)on a local basis (workplace), the risk could be partly counteracted by taking preventive measures such as using mask and globes and good ventilation in the work places. Acetanilide may pose a potential risk for workers by dust inhalation. For the sake of health protection in the work places, additional data should be accumulated with respect to repeated dose toxicity, reproduction toxicity and developmental toxicity, etc. It is, therefore, re-commended that acetanilide should be a candidate for further work to supplement the lacking data until it is proved to be safe in the occupational health aspects.

Initial Risk Assessment of Acetanilide with Respect to Ecological Integrity

Lee, Su-Rae,Choi, Seon-Ju,Lee, Mi-Kyung,Nam, U-Kyung,Chung, Sun-Hwa,Seog, Geum-Su,Park, Kwang-Sik,Kim, Kyun,Kim, Yong-Hwa 한국환경독성학회 2000 환경독성보건학회지 Vol.15 No.1

아세트아닐리드는 의약품과 염료의 합성과정에서 중간체로서 공기와 폐수를 통하여 환경 중에 방출 될 수 있다. 아세트아닐리드는 호기적 조건하에서 신속히 생분해되고 OH래디컬의 존재하에 간접적으로 광분해된다. 생물농축계수는 4.5로 추정되므로 수생생물에서의 생물농축은 낮을 것으로 예상된다. 아세트아닐리드에 관한 생태독성학적 데이터 조사결과 4종 어류에 대한 급성독성치만 보고되어 있으며, EUSES시스템에 의하면 어류에서의 최저 PNEC값 (예상 무작용농도)은 0.01㎎/l이고 표면수에서의 PEC값(예상 환경농도)은 지역수준에서 최악의 경우 9.1?0^(-5)㎎/l이다. 지역수준에서 표면수에 대한 아세트아닐리드의 RCR(위해성지수)은 9.1?0^(-3)으로 추정되어 어류에 대한 안전성을 충분하다. 그러나 국지 수준에서의 RCR은 물과 침적물에서 각각 1.3과 1.6이므로 제조공장 주변에서는 생태독성 위험이 존재할 것으로 추정된다. 아세트아닐리드의 환경위해성 평가를 보다 정확하기 하기 위해서는 물벼룩과 조류에 대한 급성독성 자료가 보완되어야 할 것이며, 따라서 이에 대한 실험이 진행되어야 할 것으로 사료된다. Acetanilide may be released into the environment through air and wastewater from its production and use sites as an intermediate in the synthesis of phar-maceuticals and dyes. Acetanilide is biodegraded rapidly under aerobic conditions and decomposed by indirect photolysis in the presence of OH radicals. An estimated bioconcentration factor of 4.5 suggests that bioaccumulation in aquatic organisms is low. Ecotoxicological data on acetanilide exist on acute toxicity to fishes of 4 species only. According to the EUSES system, the lowest PNEC (Predicted no effect concentration) in fishes is 0.01 mg/l and PEC (Predicted environmental concentration) for surface water on a regional scale is 9.1?0^(-5) mg/l as the worst case. RCR (Risk characterization ratio) of acetanilide for surface water on a regional scale was estimated as 9.1?0^(-3), which is safe enough C(=)or fishes. RCR on a local basis slightly exceeds the value I in water and sediment; that is, 1.3 and 1.6, respectively, which suggests the existence of ecotoxicological risk at the vicinity of the manufacturing site. For the refinement of environmental risk assessment on acetanilide, more data should be collected regarding prolonged fish toxicity, acute toxicity to ward daphnia and algae. It is, therefore, recommended that acetanilide should be a candidate for further work to supplement the lacking data until it is proved to be safe in the ecotoxicological aspects.