인삼노두 추출물의 위염 및 위궤양에 대한 효과

정기화,이은방,정춘식,Jung, Ki-Hwa,Lee, Eun-Bang,Chung, Chun-Sik 한국생약학회 1996 생약학회지 Vol.27 No.4

In a preliminary screening of plant extracts for the antigastritic and antiulcer actions in rats, the extracts of head of Panax ginseng Radix showed positive activity in HCl ethanol-induced gastric lesion. Among the systematic fractions of hexane, chloroform, butanol and water, the most potent butanol fraction reduced significantly HCl ethanol-induced gastric lesion at the oral dose of 500 mg/kg. In pylorus ligated rats, hexane and butanol fraction showed decreases in the volume of gastric secretion and acid output, of which effects were stronger in butanol fraction. Further assays with butanol fraction disclosed that it significantly suppressed the aspirin-induced and Shay ulcer. The butanol fraction at the intraduodenal dose of 500 mg/kg showed significant stimulation of mucus secretion.

수종추출제제(數種抽出製劑)의 항피로효과(抗疲勞效果)에 관(關)한 연구(硏究) 제3보(第3報) -Polygonatum officinale, Poria cocos 및 Asparagus cochinchinesis의 추출제(抽出劑)-

정기화,Jung, Ki-Hwa 한국약제학회 1985 Journal of Pharmaceutical Investigation Vol.15 No.4

Total ethanol extract of chinese drugs was administered to mice for 4 weeks (small dosage group) and 3 days (large dosage group), and its antifatigue effects and hepatonic activities were compared with those of P. ginseng and A. sessiliflorum. 1. In the long-term administration of extracts, the swimming performance peaked at 2 weeks, and the recovery effects on the intoxicated liver appeared to be significant in the extracts of P. officinale, P. cocos and A. cochinchinensis among tested chinese drugs. 2. The large dose administration of extracts gave high improvement of recovery effect on the intoxicated liver.

수종추출체제(數種抽出製劑)의 항피로효과(抗疲勞效果)에 관(關)한 연구(硏究) (제 2 보)(第 2 報) -Poria cocos 및 Sophora japonica의 추출제(抽出劑)-

정기화,Jung, Ki-Hwa 한국약제학회 1984 Journal of Pharmaceutical Investigation Vol.14 No.4

Extract of drugs in commerce was administered to mice for 3 days and its fatigue effects were compared with those of P. ginseng extract and A. sessiliflorum extract. Mice were used for these experiments divided into ethyl alcohol and water extract of P. cocos, S. japonica, P. multiflorum, L. chinense, A. cochinchinensis, S. indicum, N. nucifera were given ordinary dose orally. For antifatigue test, swimming performance were determined after administration of the extract The group of P. cocos gave high improvement of the swimming performance in ethyl alcohol extract group. And the group of S. japonica an improvement was also observed in water extract group.

Corticosteroid 가 Streptozotocin 유발 당뇨 흰쥐와 태자에 미치는 영향

정기화(Ki Hwa Jung),정춘식(Choon Sik Jeong),주경미(Kyung Mi Joo) 한국응용약물학회 1997 Biomolecules & Therapeutics(구 응용약물학회지) Vol.5 No.3

The effect of corticosteroid on the diabetic pregnant rats and their fetuses was investigated. Streptozotocin (STZ) was injected into the pregnant rats on the fifth day of pregnancy. Dexamethasone (DXM) was injected into the pregnant rats on the 17th, 18th, 19th and 20th days of pregnancy. In prenatal rats, the body weight, an abortion rate, number of fetus, the ratio of lecithin/sphingomyelin (L/S) and the levels of blood glucose and phosphatidylglycerol (PG) were determined. In the postnatal rats, the body weight, the levels of blood glucose, fetal number, stillbirth rate, an organ weight and the levels of hepatic glycogen, protein and triglyceride were determined. The body weight of fetuses was lower in the DXM group and higher in the STZ group than the those of control group. Blood glucose of fetuses produced hypoglycemia in the STZ group compared with the control group. A significant increase in the abortion and stillbirth rates was observed in STZ group. The levels of glycogen, protein and triglyceride in fetus liver and the weight of pancreas were significantly increased in the STZ and STZ+DXM groups compared with the control group. The L/S ratio and the level of PG in the amniotic fluid were significantly decreased in STZ group compared with the control group, whereas those of the STZ+DXM group were similar to the control group. It has been observed that corticosteroid administration on the STZ-induced diabetic rats during final stage of pregnancy can prevent the respiratory depression syndrome of neonatal rats.

사염화탄소에 의한 간독성에 미치는 홍화자의 보호작용

정기화(Ki Hwa Jung),정춘식(Choon Sik Jeong) 한국응용약물학회 1996 Biomolecules & Therapeutics(구 응용약물학회지) Vol.4 No.4

The protective effect of Carthamus tinctorius L. Semen on the carbon tetrachloride induced liver damaged rats were studied. First, methanol extract was prepared and the extract was fractionated with hexane, CHCl₃, BuOH and H₂O respectively. Animals were divided into 6 groups and each group was treated with each fraction respectively. To investigate the hepato-protective effect of Carthamus tinctorius L. Semen AST, ALT, albumin, TP, cholesterol, TG, creatinine and total bilirubin values were measured in each treated group and compared with those of control group. GST activity was increased in BuOH group compared with the control group. In malondialdehyde levels, all fractions was decreased compared with the control group. In histopathologic examination, hexane and H₂O fractions of Carthamus tinctorius L. Semen observed mild degree of ballooning degeneration. The results show the protective effect of Hexane, CHCl₃, BuOH and H₂O fractions on hepatotoxicity of CCl⁴, by decreasing ALT, AST, bilirubin, cholesterol, TG and BUN. It seems that the decrease of MDA are related to the recovery effect. The protective effects of Carthamus tinctorius L. Semen fractions in hepatotoxic pathogenesis by CCl⁴ was suggested in blood chemistry analysis and histopathologic examination.

타이어 공기압에 따른 LKAS 제어성능 변화 연구

정기화(Ki hwa Jung),김순태(Soon Tae Kim) 한국자동차공학회 2011 한국자동차공학회 학술대회 및 전시회 Vol.2011 No.11

LKAS(Lane Keeping Assistant System) is an assistance system that helps maintain the lane through steering wheel operation during drowsy or careless driving or deviating from the lane are expected. Lane-keeping performance characteristics are an important factor in the behavior of the vehicle. In this paper, due to changes in tire pressure and vehicle behavior characteristics were studied. By applying the test results reflected in the controller design by implementing a more robust control results were derived.

승마추출물이 흰쥐의 사염화탄소 유발 간독성에 미치는 효과

정기화(Ki Hwa Jung),정춘식(Chun Sik Chung),노혜림(Hye Rim No) 한국응용약물학회 1996 Biomolecules & Therapeutics(구 응용약물학회지) Vol.4 No.1

The effect of Cimicifuga·heracleifolia on CCl₄-induced hepatotoxicity was investigated. Cimicifuga heracleifolia has been used to diaphoresis, antipyretics and detoxification in oriental remedy. We examined the effect of Cimicifuga heracleifolia methanol extract by blood chemical analysis and histopathologic examination. ALT and AST were decreased by 38% and 67% in pretreatment group of Cimicifuga heracleifolia compared to CCl₄ control group respectively. There were significant changes neither in total protein, albumin, triglyceride and cholesterol nor in BUN and creatinine. In histopathologic examination, there were severe necrosis and hemorrhage with infiltration of inflammatory cells around the central vein, zone 3 in the liver of CCl₄ treated rat. Ballooning degeneration of hepatocytes were frequently noted in the periphery of the hemorrhagic necrosis. In Cimicifuga heracleifolia pretreatment group, we observed mild degree of ballooning degeneration and inflammatory cell infiltration. No gross necrosis was observed. We measured malondialdehyde (MDA) formation by TBA method. It showed that the formation of MDA in Cimicifuga heracleifolia pretreatment group was decreased compared to the CCl₄, control group. We got the result of the effect of Cimicifuga heracleifolia on CCl₄-induced hepatotoxicity by decreasing serum ALT and AST. It seems that the decrease of lipid peroxidation is related to the recovery effect.

SLIF(Speed-Limit-Information-Function) 시험평가 방법에 관한 연구

정기화(Ki-hwa Jung),이동열(dong-yeol Lee),노재만(Jea-Man No),모재현(jae-hyun.mo) 대한전자공학회 2015 대한전자공학회 학술대회 Vol.2015 No.6

SLIF(Speed-Limit-Information-Function)is a driver support function which can be used to notify and warn the driver which restrictions may be effective on the current stretch of road. In this paper, Test and evaluation methods are proposed such as establishing criteria for the study. In addition, we propose a scheme that can be verified in Lab environment to replace the actual vehicle evaluation. As a result, it can improve the robust performance test of SLIF System.

Improvement of Boar Semen Quality by Sperm Selection Using Magnetic Nanoparticles

KI-Hwa Chung(정기화),Jung-Ho Son(손종호) 한국생명과학회 2016 생명과학회지 Vol.26 No.8

본 연구는 간단히 활용할 수 있는 나노 크기의 마그네틱 비드를 이용하여 정자의 품질을 향상시킬 수 있는지 여부를 규명하기 위하여 실시하였다. 돼지 정자 시료는 인공수정 센터에서 공급받아 실험실로 2시간 이내로 이송한 후 CASA 측정을 통하여 4개의 활력을 나타내는 그룹으로(1, > 90%; 2. 80~90%; 3. 70~80%; 4. < 70%) 분류하였다. 정액은 BTS 희석제를 사용하여 보존하였고, 총 정자수와 동일한 농도의 마그네틱 비드를 정액에 20분간 처리한 후, 5분간 실온에서 마그네틱 비드에 반응한 정자를 자석을 이용하여 분리하였다. 마그네틱 비드 처리 전과 후 정자의 생존율 및 활력은 CASA를 이용하여 측정하였고, 기형율과 정자응집의 정도는 현미경으로 검사하였다. 처리 후의 정자 활력은 4개 그룹 모두에서 유의하게(p<0.0.5) 높은 차이를 보였으며 처리 전에 비하여 평균 7.11% 향상되었다. 정자 활력의 변화는 처리 전 낮은 활력을 보인 그룹에서 보다 현저한 차이를 보였다(< 70% and 70~80%; 19.12±1.08% and 5.67±0.71%, p<0.0.5). 정자 활력을 VCL, VSL, VAP 및 LIN (%)로 구분한 성적에서도 유사한 패턴을 나타냈고, 이러한 현상은 활력 70% 이하를 나타낸 그룹에서 개선 효과가 더욱 뚜렷하였다. 마그네틱 비드 처리 후 정자 생존율은 처리 전에 비하여 평균 4%가 향상 되었고(p<0.0.5), 정자 기형율 또한 3.7~4.5%(p<0.0.5) 정도 감소하였다. 정자 응집의 정도 또한 마그네틱 비드 처리를 통하여 처리 전 낮은 활력을 나타낸 그룹에서 감소됨을 알 수 있었다. The objective of this study was to see if fairly simple magnetic nano-particle treatment enhances boar semen qualities. Boar semen samples were prepared from the swine AI center and samples were divided by 4 different motility groups (1, >90%; 2. 80~90%; 3. 70~80%; 4. <70%) using computer assisted sperm analysis (CASA) evaluation. Boar semen was extended using BTS extender and same number of magnetic nano-particles as total number of spermatozoa in each sample was treated for 20 min and collected for 5 min at room temperature. Sperm qualities such as motility and viability were evaluated by the CASA before and after treatment. Sperm abnormality and degree of agglutination were also evaluated under the microscopic examination before and after treatment. There were significant changes (p<0.05) on sperm motility from all 4 different groups in the average of 7.11% after treatment. The enhancement of sperm motility changes was more clear in the groups of lower sperm motile groups (<70% and 70~80%; 19.12±1.08% and 5.67±0.71%, p<0.05). The sperm motility character in terms of curvilinear velocity (VCL), straight line velocity (VSL), average path velocity (VAP) and linearity (LIN, %) showed also similar pattern but motility enhancement wear more clear in below 70% motile group. Average sperm viability was increased to 4% by magnetic nano-particles (p<0.05). The percentage of sperm abnormality was also reduced significantly (p<0.05) to the range of 3.7~4.5% before after treatment. The degree of sperm agglutination was also reduced in lower motility groups by the magnetic nano-particle purification.

Protective Effects of 3-Aralkylthio-6-allylthiopyridazine Derivatives on Aflatoxin B1-Induced Hepatotoxicity in Rat

Eun-Jung Cho(조은정),Hae-Sun Park(박혜선),Soon-Kyoung Kwon(권순경),Ki-Hwa Jung(정기화),Hea-Soon Shin(신혜선) 환경독성보건학회 2006 한국독성학회 심포지움 및 학술발표회 Vol.- No.-