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제주지역의 akabane virus에 대한 역학조사 및 원인체 분리동정
강완철 ( Wan Cheul Kang ),김은주 ( Eun Joo Kim ),현관종 ( Kwan Jong Hyun ),전창익 ( Chang Ik Cheon ),김희석 ( Hye Seck Kim ),이두식 ( Du Sik Lee ) 한국가축위생학회 2000 韓國家畜衛生學會誌 Vol.23 No.1
In this experiment, we studied the sero-positive rate of akabane virus in cattle from Jeju-do and analyzed the seroepidemiological features. In an analysis of 1051 samples, the positive rate for neutralizing antibody in sera collected in nine regions on Jeju-do was 56.7%. The rate varied with the region. The positive rate was 69.6% in Aewol, 63.1% in Jeju city, 54.4% in Anduck, 51.0% in Hallim, 69.8% in Jocheun, 47.6% in Pyosun, 40% in Daejeong, 30.0% in Flankyung, 71.6% in Namwon, 24.5% in Sungsan, 83.3% in Seokypo and 44.5% in Gujwa, respectively. The rate also depended on the age of the cattle. The positive rate was 67.2% in calves 0- to 12-month old, 48.3% in cattle 13- to 24-month old, 65.4% in cattle 25- to 36-month old, arid 65.4% in cattle more than 37 months old. To isolate the virus from calves with malformations including arthrogryposis and hydranencephaly, cerebral homogenates were inoculated into Vero cells, which were determined for cytopathic effect (CPE). Vero cells with CPE were examined for Akabane virus using an electron microscope (EM) and indirect immunofluorescent antibody test (WA). Typical virus particles with a width of 90-l3Onm and specific immunofluorescence in the cytoplasm of infected cells were sought for identification.
김은주 ( Eun Joo Kim ),정경주 ( Kyong Joo Jeong ),김진영 ( Jin Young Kim ),김진희 ( Jin Hoe Kim ),전창익 ( Chang Ik Cheon ),이두식 ( Du Sik Lee ),임윤규 ( Yoon Kyu Lim ) 한국가축위생학회 2001 韓國家畜衛生學會誌 Vol.24 No.1
In order to investigate the relationship between milk hygienic quality and some environmental factors such as the herd size and types of milking machines, we sampled and examined the level of total bacterial count, coliforms, Staphyococcus aureus, somatic cell counts(SCC) and fat rates in raw milk. Of the 84 dairy farms, the prevalence of level on number of standard plate count over l00,000cfu/mℓ and coliforms over 1,000cfu/mℓ in bulk milk were 25.0% and 15.6%, respectively. Also, 2 farms(2.4%) were exceed the level on number of 500cfu/㎖ S aureus in raw milk. The prevalence of dairy herd with first grade of total bacterial count(TBC) according to bucket, pipe line and parlour milking system was 40.0%, 74.0% and 84.0%, respectively. The prevalence of dairy herd with first grade of TBC according to grade 1, 2 and 3 by SCC was 77.8%, 83.2% and 69.2%, respectively. Therefore, the relationships between hygienic quality in raw milk and the herd size, types of milking machines, were significant. In conclusion, this study could be overemphasized the importance of herd management condition for milk hygienic quality.
김은주 ( Eun Joo Kim ),강원명 ( Won Myoung Kang ),정경주 ( Kyong Joo Jeong ),김우택 ( Woo Taek Kim ),김진희 ( Jin Hoe Kim ),전창익 ( Chang Ik Cheon ),임윤규 ( Yoon Kyu Lim ) 한국가축위생학회 2000 韓國家畜衛生學會誌 Vol.23 No.4
The growth of bacteria on the surface of the meat was monitored to investigate the relationships between microbiological quality and some environmental factors such as the chilling temperature, alcohol spraying, and transport in slaughter process of pigs. The temperature changes of the surface and inner part of pork carcass were monitored with GreenTrack system during the process of chilling and transport. Of the 100 pigs tested, the prevalence of level on number of standard plate count (SPC) less than i? CFU/cm2 and Escherichia coil less than 102 CFU/cm2 in pig were 82% and 80%, respectively. Surface bacterial numbers are decreased in the course of chilling process of the carcass. Alcohol spray process before packing meat also could decrease the surface bacterial count. In conclusion, this study could be overemphasized the importance of relationship between microbiological quality and refrigerating temperature in the process of refrigeration and cutting.