지속가능발전교육의 관점을 적용한 음식주제 활동이 유아의 친사회적 행동, 환경친화적 태도, 경제 생활습관에 미치는 영향과 의미

전소영 ( So Young Chun ),김은정 ( Eun Jung Kim ) 한국아동교육학회 2016 아동교육 Vol.25 No.1

본 연구의 목적은 지속가능발전교육의 관점을 적용한 음식주제 활동이 유아의 친사회적 행동, 환경친화적 태도, 경제 생활습관에 미치는 영향을 분석하고, 활동과정에서 실행된 의미 있는 경험을 살펴보는 것이다. 본 연구의 대상은 만 3, 4, 5세 혼합 연령반의 11명이다. 검사도구는 친사회적 행동, 환경친화적 태도, 경제 생활습관 검사 도구를 사용하였다. 본 연구의 결과, 지속가능발전교육의 관점을 적용한 음식주제 활동은 유아의 친사회적 행동, 환경친화적 태도, 경제 생활습관에서 모두 유의미한 차이가 있었고, 활동과정에서 유아의 친사회적 행동, 환경친화적 태도, 경제 생활습관에서 의미 있는 경험이 실행되고 있었다. 이러한 결과는 지속가능발전교육의 관점을 적용한 음식주제 활동이 유아의 친사회적 행동, 환경친화적 태도, 경제 생활습관에 긍정적인 효과가 있음을 시사해 준다. The purpose of this study is to analyze the effects and meanings of the food project activities based on education for sustainable development(ESD) on children``s pro-social behaviors, eco-friendly attitudes, and economic habits. The subjects of the study are 11 children aged from 3 to 5 years old, of a coed class in a daycare center. The research measurements are the measurements used to examine the social behaviors, eco-fiendly attitudes, economic habits related to the views of society, environment, and economy. Based on the results of this study, it could be implicated that the food focused activities based on ESD can be positively effective on children``s pro-social behaviors, eco-friendly attitudes, and economic habits.

줄기세포를 이용한 조직공학적 치아재생 동향

전소영 ( So Young Chun ),이효정 ( Hyo Jung Lee ),최영애 ( Young Ae Choi ),김재영 ( Jae Young Kim ),조제열 ( Je Yeol Cho ),신홍인 ( Hong In Shin ),박의균 ( Eui Kyun Park ) 한국조직공학과 재생의학회 2009 조직공학과 재생의학 Vol.6 No.1

This review focuses on the current understanding of tooth regeneration using embryonic tooth budderived tissues/cells and adult stem cells, and tissue engineering technologies combined with biomaterials. Tooth development is achieved by sequential and reciprocal interactions between the oral epithelium and the mesenchyme. Especially growth factors, transcription factors and extracellular matrix proteins play key roles during tooth development process. To achieve successful bio-engineered tooth, several different sources for epithelium and mesenchymal stem cells are needed to be assessed their potential efficacy. Next, discussion focuses on cell culture and implantation methods, particularly, medium supplements, gene transfection, chimeric tooth bud and optimized implant regions. At the end, we introduce the various biomaterials designed via biomimetic strategies for tooth regeneration applications. Although currently these approaches have several limitations including abnormal morphology of bio-engineered tooth, the tissue engineering approaches for dental regeneration using stem cells is the significant challenge in dental field. Therefore, the knowledge of the stem cells as well as the biomaterials will help our understanding for the tooth regeneration.

두경부암 세포주에서 TPEF 유전자의 methylation 변이

전소영(So-Young Chun),김정옥(Jung-Ock Kim),홍수형(Su-Hyung Hong),정유경(Yu-Kyung Chung),장현중(Hyun-Jung Jang),손윤경(Yoon-Kyung Shon),김정완(Jung-Wan Kim) 대한구강악안면외과학회 2005 대한구강악안면외과학회지 Vol.31 No.6

Head and neck squamous cell carcinoma (HNSCC) is the sixth most common malignancy worldwide. The molecular mechanisms involved in the development and progression of these carcinomas are not well known. Abnormalities of genomic methylation patterns have been attributed a role in carcinogenesis and local de novo methylation at tumor suppressor loci was held to be involved in silencing of tumor suppressor genes. Using Ms APPCR, we previously isolated a hypermethylated fragment corresponded to the 5’end of TPEF gene from primary liver and lung cancer cells. To confirm the inactivation of TPEF gene by hypermethylation in HNSCC, we investigated correlation between methylation pattern and expression of TPEF in 10 HNSCC cell lines. In methylation analysis such as combined-bisulfite restriction analysis(COBRA) and bisulfite sequencing, only RPMI 2650 showed none methylated pattern and another 9 cell lines showed dense methylation. The TPEF gene expression level analysis using RT-PCR showed that these 9 cell lines had not or significantly low expression levels of TPEF as compared with RPMI 2650. In addition, the increase of TPEF reexpression by 5-AzaC as demethylating agent in 9 cell lines also indicated that TPEF expression was regulated by hypermethylation. These results of this study demonstrate that epigenetic silencing of TPEF gene by aberrant methylation could play an important role in HNSCC carcinogenesis.

해양심층수의 타라소테라피 적용을 위한 효능검정

전소영(So-Young Chun),이규식(Kyu-Shik Lee),남경수(Kyung-Soo Nam) 한국해양환경·에너지학회 2017 한국해양환경·에너지학회 학술대회논문집 Vol.2017 No.4

탈세포 장기 지지체의 제조 및 분석기법

전소영 ( So Young Chun ),오세행 ( Se Heang Oh ),유지 ( James J Yoo ),권태균 ( Tae Gyun Kwon ) 한국조직공학과 재생의학회 2015 조직공학과 재생의학 Vol.12 No.1s

Organ transplantation has often been successful for treatment of end-stage organ failure. However, the shortage of donor organ still remains problematic in clinical practices. As an alternative, the tissue-engineering approach for functional organ replacement has been extensively studied. More recently, decellularized organs have been emerged as a promising scaffold for reconstruction of the complicated organs (e.g., heart, liver, lung and kidney). The ideal decellularized organ scaffolds need to contain extracellular matrix (ECM), bioactive molecules, vascular systems and tissue microarchitecture. To fulfill these requirements, physical, chemical, and biological techniques have been adapted in the process of organ decellularization. In this review, the representative techniques for the organ decellularization and their characterization as well as considerations for implantation are discussed.

자가골이식재로서 RGD펩타이드가 고정된 탈회상아질

전소영 ( So Young Chun ),( Bodhraj Acharya ),신홍인 ( Hong In Shin ),박의균 ( Eui Kyun Park ) 한국조직공학·재생의학회 2010 조직공학과 재생의학 Vol.7 No.1

The purpose of this work is to modify demineralized dentin particle (DDP) with cell adhesion peptide (Arg-Gly-Asp; RGD) for bone regeneration. DDP was prepared with EDTA chelating agent and proved cell proliferation and mineralization effect in vitro. FT-IR analysis showed that DDP contained rich amine group on the surface. Endogenous amine group was applied for immobilization of a cell adhesive peptide. DDP surface was modified with RGD peptide. In mouse calvaria defect model, RGD immobilized DDP showed osteoconduction. Tartrateresistant acid phosphatase (TRAP) staining showed that the implanted DDP was dissolved by osteoclasts. Von Kossa staining proved mineral deposition on the defected area. Therefore, these results suggested that the surface modified DDP can be used as a suitable bone autograft material.

대장암의 예후와 Apoptosis 활성

전소영(So-Young Chun),배옥석(Ok-Suk Bae),김종봉(Jong-Bong Kim) 대한의생명과학회 1998 Biomedical Science Letters Vol.4 No.2

본 연구에서는 genomic DNA 전기영동과 TUNEL labeling법을 이용하여 정상대장조직, 대장암조직, 대장암인접림프절조직과 대장암환자 혈액에서 apoptosis 발현을 분석하였다. 정상대장조직 37례중 4례에서, DNA ladder가 확인되었고, 암조직은 47례중 20례에서, 림프절조직은 15례중 5례에서 나타났으며 대장암환자혈액은 7례 모두에서 발현되지 않았다. TUNEL labeling법을 이용한 조직상의 in situ apoptosis 발현은 암조직과 림프절조직에서 확인되었다. 따라서 본 연구에서는 대장암이 진행됨에 따라 apoptosis 발현비가 증가되었으므로 apoptotic index가 대장암 발현과 관련되어 있는 듯 하며 예후예측지표자로서 이용될 가능성이 있다고 사료된다. We studied on the expression of apoptosis in colorectal cancer, lymph node, their corresponding normal mucosa and colorectal cancer patient's blood by genomic DNA electrophoresis and TUNEL labeling method. From 7 cases among 37, 20 cases among 47 and 5 cases among 15, DNA ladders were expressed in normal tissues, colorectal tissues and lymph node tissues, respectively. A DNA ladder was not observed in 7 cases of colorectal cancer patients blood. In case of TUNELlabeling, we could observe TUNEL color espression in colorectal cancer and lymph node tissues. As these result suggest that apoptotic index may be associated with the colorectal cencer development, and mat be used as a prognostic indicator but further evaluations will be needed.

키토산올리고당의 유방암 전이억제능에 미치는 해양심층수의 효과

권윤숙 ( Yun Suk Kwon ),전소영 ( So Young Chun ),이도형 ( Do Hyung Lee ),남경수 ( Kyung Soo Nam ) 한국키틴키토산학회 2013 한국키틴키토산학회지 Vol.18 No.2

본 연구에서는 해양심층수가 키토산올리고당의 암전이 인자 및 혈관신생 조절인자의 발현 억제 효과에 미치는 영향을 평가하기 위하여 MCF-7을 이용하여 MMP-9, uPA, uPAR 및 VEGF의 발현을 평가하였다. 그 결과 3 mg/mL의 키토산올리고당 단독처리에 의한 MMP-9와 uPA의 발현저해 효과는 경도 400 이상의 해양심층수와 혼합 처리하였을 때 발현저해 효과가 더욱 향상되는 것으로 나타났다. 뿐만 아니라 uPAR의 경우 키토산올리고당에 의한 발현저해 효과가 없었으나 경도별해양심층수와 혼합하여 처리하였을 때 그 저해효과가 유의적으로 더욱 증가하였다. MMP-9, uPA 및 uPAR은 세포외기질과 기저막을 분해하여 암세포가 다른 조직으로 전이되는 과정에 반드시 필요한 단백질이다. 따라서 키토산올리고당과 해양심층수의 혼합처리에 의한 이들 효소의 발현억제는 암의 전이를 억제 및 예방할 수 있는 가능성을 보여주는 것을 의미한다. 또한 VEGF는 혈관신생을 조절하는 주요인자로 해양심층수와 키토산올리고당의 혼합 처리에 의한 이의 발현 저해는 새로운 혈관의 생성을 저해시킴으로써 암세포의 성장 및 전이를 억제 또는 예방할 수 있음을 추측할 수 있다. 이상의 해양심층수에 의한 키토산올리고당의 유방암 세포 전이인자 발현억제능 증강효과는 Ca:Mg의 섭취비율이 높은 경우 결장직장종양 (colorectal neoplasia)의 발병위험이 높은 반면 Ca:Mg 섭취비율이 낮을 때 그 위험성이 감소한다고 밝혀진 연구결과에 비춰볼 때, 해양심층수에 존재하는 Mg 2+ 및 Ca2+의 비율에 의한것으로 추측된다 (27). 따라서 본 연구는 해양심층수와 키토산올리고당의 혼합사용을 통해 이미 많은 연구에서 밝혀진 키토산올리고당의 암전이 억제효과를 더욱 향상시키는 효과가 있음을 보여주고 있으며, 유방암의 예방, 특히 전이억제를 위한 이들 혼합액의 새로운 응용 가능성을 제시하는 바이다. 하지만 이에 대한 명확한 효과는 해양심층수의 Mg과 Ca의 농도 및 비율 조절을 통해 추가적인 연구가 진행되어야 할 것으로 생각된다. Metastasis is a major cause leading to high mortality to breast cancer patients. Invasiveness of breast cancer cells is associated with matrix metalloproteinases (MMPs), urokinase type plasminogen activator (uPA), and its receptor (uPAR), and angiogenesis modulated by vascular endothelial growth factor (VEGF) is necessary for growth of cancer cells in distant site. In order to evaluate the effect of deep-sea water (DSW) on antimetastatic effect of chitosan oligosaccharide (COS-L, MW ≤ 1,000) in MCF- 7 human breast cancer cells, we estimated inhibitory effect of COS-L (0.1~5 mg/mL) dissolved in hardness 0 (H 0) DSW on TPAinduced MMP-9 expression. And then expression changes of TPA (phorbol-12-myristate-13-acetate)-induced MMP-9, uPA, uPAR, and VEGF by COS-L (3 mg/mL) in DSW with various hardness (200, 400, 800, 1,500) were also assessed. Expression of MMP-9 induced by TPA was diminished in a range of 3~5 mg/mL of COS-L. Moreover Interestingly, DSW of H 400~1,500 significantly enhanced inhibitory effect of COS-L on expressions of TPA-induced MMP-9, uPA, uPAR. In the case of VEGF, DSW of over H 800 had a significant inhibitory effect on the expression. The results of this study indicate that DSW promotes antimetastatic effect of COS-L in MCF-7 human breast cancer cells by decreasing expression of metastastic factors (MMP-9, uPA, uPAR) and angiogenic factor (VEGF).

신장재건을 위한 신장 세포외기질 지지체 개발

채선영 ( Seon Yeong Chae ),전소영 ( So Young Chun ),박민 ( Min Park ),장유진 ( Yu Jin Jang ),김진래 ( Jin Rae Kim ),오세행 ( Se Heang Oh ),이진호 ( Jin Ho Lee ),송필현 ( Phil Hyun Song ),권태균 ( Tae Gyun Kwon ),김태환 ( Tae Hwan 한국조직공학과 재생의학회 2014 조직공학과 재생의학 Vol.11 No.1s

The innate extracellular matrix (ECM) scaffolds can be a promising scaffold for regeneration of complex organ such as heart, liver and kidney. They possess intact 3-dimentional architecture and biochemical components that allow to access to the organ`s capillary network. In this study we have developed a porcine renal ECM scaffold and analyzed its physical and biochemical characteristics including biocompatibility for human kidney regeneration. A segmented porcine kidney cortex was obtained and treated with 1% (v/v) Triton X-100 (Triton) or sodium dodecyl sulfate (SDS) in a shaking chamber, and rinsed with distilled water. After confirmation of decellularization with H&E stain, the matrix was lyophilized and sterilized. Scanning Electron Microscope (SEM) analysis showed that both scaffolds were preserved with proper architecture including porosity for cell adhesion and composition of the renal ECM. The water uptake ability of the Triton treated scaffold was higher than that of SDS treated one. The maximum compressive strength of Triton was lower than SDS treated scaffolds and that correlates with the results of porosity and water uptake analysis. In ATR-IR analysis, both scaffolds showed a peak at 3445-3446 cm-1 and that indicates the presence of amide II (-NH). Triton treated scaffold demonstrated that there are richer contents of ECM proteins and growth factors compared to SDS treated one. When scaffolds were seeded with primary human kidney cells, Triton treated scaffold showed 2.66 times higher number of adherent cells than SDS treated one at 24 hrs postseeding. On a CCK-8 analysis, the Triton treated scaffold showed significantly higher cell viability and proliferation rate than that of SDS treated one. Both scaffolds had no tumorigenecity for 8 weeks in vivo analysis. In conclusion, we successfully developed porcine renal ECM scaffold and confirmed that there is a great potential of porcine renal ECM scaffold to be used as human kidney regeneration. We also verified that 1% Triton X-100 is more suitable decellularizing agent than SDS regarding structural, biochemical integrity and biocompatibitilty of the scaffold. To support our findings and human application of practical regeneration, we are planning to perform in vivo experiment for kidney regeneration near future.

해양심층수가 키토산올리고당의 Superoxide Anion Radical 소거에 미치는 영향

이민구 ( Min-gu Lee ),전소영 ( So-young Chun ),이규식 ( Kyu-shik Lee ),남경수 ( Kyung-soo Nam ) 한국키틴키토산학회 2017 한국키틴키토산학회지 Vol.22 No.1

본 연구에서는 키토산올리고당(COS-L)의 superoxide anion radical 소거능에 미치는 해양심층수(DSW)의 영향을 ESR(electron spin resonance) spectroscopy를 사용하여 확인하였다. 먼저, 본 실험에 사용한 키토산올리고당 농도와 해양심층수 경도에서는 HaCat 인간상피세포에 별다른 독작용을 나타내지 않았다. 따라서 키토산올리고당과 해양심층수를 혼합 처리하여 superoxide anion radical 소거능을 확인하였다. 그 결과 키토산올리고당과 해양심층수 혼합처리 조건에서 키토산올리고 당에 의한 superoxide anion radical 소거능이 해양심층수의 경도의존적으로 증가됨을 알 수 있었다. 특히 경도 750 이상의 해양심층수는 키토산올리고당에 의한 소거 효과를 더욱 증가시키는 것으로 나타났다. 결론적으로 본 연구에서 해양심층수는 고경도(750 이상)에서 키토산올리고당의 superoxide sca-venging activity를 더욱 상승시키는 효과를 보여주고 있다. 앞으로 다양한 spin trap을 사용한 추가적인 free radical 생성에 미치는 연구가 수행되어야 할 것으로 생각된다. In this study, we investigated the effect of deep-sea water (DSW) on chitosan oligosaccharide (COS-L)-mediated superoxide anion radical scavenging activity using electron spin resonance (ESR) spectroscopy. At first, we observed anti-oxidative activity of COS-L in a concentration-dependent manner (25~750 μg/mL). Also, superoxide scavenging activity of DSW was found in a hardness-dependent manner (hardness 250~1,000). The concentrations of COS-L and the hardness of DSW used in the experiment were not found to be toxic to the HaCat cells, respectively. Based on the results, we evaluated effects of DSW on superoxide scavenging activity of COS-L (250 μg/mL). Then, COS-L was dissolved in DSW of various hardness and then the mixtures were added in 5,5-dimethyl-1-pyrroline-N-oxide (DMPO) solution. Next, the mixtures added in DMPO solution were exposed with ultraviolet light to produce superoxide. The result showed that superoxide anion radical scavenging activity induced by COS-L (250 μg/mL) were significantly increased by DSW with hardness dependently (the range of hardness was 250, 500, 750 and 1,000, respectively). These data suggest that mixtures of COS-L (250 μg/mL) and DSW should increase superoxide scavenging activity by spin trap. Consequently, DSW effectively enhanced superoxide scavenging activity of COS-L.