한국산 외생균근균의 배양 특성

전성민,가강현,Jeon, Sung-Min,Ka, Kang-Hyeon 한국균학회 2015 韓國菌學會誌 Vol.43 No.1

균근성 버섯은 나무의 뿌리에 외생균근을 형성하는 공생균류로서 인류에게 맛있는 버섯을 제공하고 있다. 국립산림과학원에서는 이러한 균근성 버섯류의 인공재배뿐만 아니라 이들의 순수분리 배양과 보존, 기초 배양 특성 조사등에 집중하여 연구를 진행하고 있다. 본 총설은 국립산림과학원에 보존된 많은 균근성 버섯들의 배양특성을 소개한 것으로 차후 균근성 버섯을 연구하고자 하는 이들이 유용한 산림자원을 발굴하는 데에 도움이 되는 기본 정보가 되길 바란다. Many ectomycorrhizal fungi provide delicious foods for humans as symbiotic fungi forming ectomycorrhizas on roots of trees. Korea Forest Research Institute (KFRI) is focused on studying the pure culture, conservation, and fundamental characteristics of ectomycorrhizal fungi as well as their artificial cultivation. In this review, we described the cultural characteristics of many ectomycorrhizal fungi that are preserved in the cold room of KFRI. The aim of this article is to provide basic information that will be useful in investigating good forest resources for any researchers who are interested in this topic.

한국산 외생균근균의 배양 특성

전성민 ( Sung Min Jeon ),가강현 ( Kang Hyeon Ka ) 한국균학회 2015 Mycobiology Vol.43 No.1

Many ectomycorrhizal fungi provide delicious foods for humans as symbiotic fungi forming ectomycorrhizas on roots of trees. Korea Forest Research Institute (KFRI) is focused on studying the pure culture, conservation, and fundamental characteristics of ectomycorrhizal fungi as well as their artificial cultivation. In this review, we described the cultural characteristics of many ectomycorrhizal fungi that are preserved in the cold room of KFRI. The aim of this article is to provide basic information that will be useful in investigating good forest resources for any researchers who are interested in this topic.

방사선(학)과의 작업종사자와 수시출입자의 교내 실습에 따른 피폭선량에 대한 고찰

전성민(Seong-Min Jeon),이용기(Yong-Ki Lee),안성민(Sung-Min Ahn) 한국방사선학회 2021 한국방사선학회 논문지 Vol.15 No.3

본 연구에서는 교내 실습 중 방사선(학)과 에 지정된 방사선작업종사자 및 수시출입자의 피폭 정도를 분석하여 방사선(학)과에 대한 원자력안전법의 방사선 방호에 대한 타당성과 최적화에 대한 기초 연구에 목적을 두었다. 연도별 작업종사자의 평균 피폭선량 2014년과 2016년에 0.01 mSv로 가장 낮은 수치가 나타났으며. 가장 높은 수치는 2018년도로 0.12 mSv이다. 연도별 수시출입자의 평균 피폭선량은 2018년 0.013 mSv로 가장 낮은 수치를 보였으며, 2016년 0.022 mSv로 가장 높은 수치가 나타났다. 본 연구를 통하여 방사선발생장치만을 사용하는 대학의 담당교수, 실습 조교 및 방사선(학)과의 학생들은 교내실습 과정에서 받는 연간 피폭선량은 일반인의 선량한도인 1mSv에도 미치지 못하는 수준이다. 그러므로 방사선(학)과 학생들의 피폭선량이 일반인의 선량한도 보다 낮게 나오는 시점에서 현재 원자력안전법의 안전규제는 과도한 규제라고 판단된다. 따라서 현재의 원자력안전법에서 방사선 발생장치의 규정을 개정하거나 대학의 재학생에 대한 방사선안전관리 체계를 수정하는 것은 필요하다고 사료된다. In this study, the purpose of this study was to analyze the degree of exposure of radiation workers assigned to the Department of Radiology and frequent visitors during on-campus practice, and to conduct a basic study on the feasibility and optimization of the radiation protection of the Nuclear Safety Act for the Department of Radiology. . The average exposure dose of occupational workers by year was 0.01 mSv, the lowest in 2014 and 2016. The highest figure was 0.12 mSv in 2018. The average exposure dose of frequent visitors by year was the lowest at 0.013 mSv in 2018, and the highest at 0.022 mSv in 2016. According to this study, the annual exposure dose received by professors, practical assistants, and students in the department of radiology (department) who use only radiation generators in the course of in-school practice is less than 1 mSv, which is the dose limit for the general public. Therefore, at the time when the radiation dose of students in the Department of Radiology is lower than the dose limit of the general public, the current safety regulation of the Nuclear Safety law is judged to be excessive regulation. Therefore, it is considered necessary to revise the regulations for radiation generators in the current Nuclear Safety law or to revise the radiation safety management system for university students.

순수배양 시 외생균근균의 질소원 요구성과 선호도

전성민 ( Sung Min Jeon ),기강현 ( Kang Hyeon Ka ) 한국균학회 2013 韓國菌學會誌 Vol.41 No.3

The nitrogen source-requirement and preference of ectomycorrhizal fungi (42 strains belong to 13 genera) collected from Korean forests were investigated by incubating mycelial cells in four nitrogen source test media for 56 days. The nitrogen requirement was relatively high in five strains (Heimipoprus japonicas (KFRI 1224), SuillUs luteus (KFRI 1232), Tylopilus castaneiceps (KFRI 1383), SUillU5 granulates (KFRI 1997), Rhizopogon sp. (KFRI 1434)) in comparison with other strains. Forty-five percent of ectomycorrhizal strains including Boletus griseus (KFRI 1362) preferred the ammonium-form of inorganic nitrogen source for mycelial growth, whereas thirty-six percent of tested strains including Suillus grevillei (KFRI 1125) preferred the nitrate-form as an inorganic nitrogen source.

한국산 가송이(가칭)의 균사생장 특성과 소나무에 외생균근 형성

전성민 ( Sung Min Jeon ),가강현 ( Kang Hyeon Ka ),홍기성 ( Ki Sung Hong ) 한국균학회 2014 韓國菌學會誌 Vol.42 No.4

Tricholoma bakamatsutake is one of the edible ectomycorrhizal mushrooms as an allied species of Tricholoma matsutake. This is the first report on physical characteristics of T. bakamatsutake strains collected from Quercus mongolica forests in Korea. The pure cultures of these strains were isolated from the tissues of fruit bodies and the culture characteristics were investigated under different conditions (media, temperatures, nitrogen sources). Most strains showed the highest mycelial growth on potato dextrose agar (PDA) at 20 or 25℃. Two strains of T. bakamatsutake preferred the ammonium-form rather than the nitrate-form as an inorganic nitrogen source. T. bakamatsutake showed significantly slower mycelial growth when compared with T. matsutake from a Korean forest, although the optimum culture conditions for the two allied species were similar. We also tested the ability to form mycorrhizae as well as cellulase activity of T. bakamatsutake. All strains showed cellulase activity on a carboxymethylcellulose (CMC) agar plate. The mycorrhizae on axenic Pinus densiflora seedlings were formed by two strains of T. bakamatsutake after 3 or 8 months of inoculation. P. densiflora seedlings inoculated with T. bakamatsutake had a much higher biomass than un-inoculated seedlings.

균근 형성과 소나무 유묘 생장이 우수한 송이 균주의 선발

전성민 ( Sung-min Jeon ),가강현 ( Kang-hyeon Ka ) 한국균학회 2016 韓國菌學會誌 Vol.44 No.3

송이산의 방치, 기주식물의 질병, 산불, 기후변화 등 다양한 원인에 의해 국내외 송이 생산량이 감소하고 있어 송이균의 생태적 특성을 이용한 인공재배 기술 개발이 필요한 실정이다. 본 연구에서는 송이 접종묘 생산에 적합한 균주를 선발하기 위해 무균 배양병 내에서 소나무 유묘와 송이균 간의 외생균근 합성을 시도하였다. 송이 균근 형성률은 총 19개 균주 중 5개 균주(NIFoS 421, 434, 1681, 1984, 2001)가 80% 이상을 나타냈다. 7개 송이 균주(NIFoS 434, 441, 561, 562, 1016, 1807, 1812)는 평균 3.0 이하의 S/Rratio를 나타내었고, 소나무 유묘 지상부의 생물량이 지하부보다 2.0~4.8배 정도 높았다. 8개 균주(NIFoS 441, 561, 562, 1016, 1807, 1812, 1984, 2001)는 유묘의 지상부 부피 생장에, 3개 균주(NIFoS 441, 562, 1812)는 균근을 형성하면서 지하부 부피 생장을 돕는 것을 알 수 있었다. 결론적으로 시험균을 접종한 지 6개월 후, 총 19개 시험균 중 4 균주(NIFoS 434, 561, 1984, 2001)는 타 균주들에 비해 송이균의 균근 형성력과 소나무 유묘 생장력이 우수한 것으로 나타나 송이 접종묘 제작에 있어 이들의 활용이 기대된다. Domestic and international production of Tricholoma matsutake has decreased owing to matsutake forests being left alone, host plant disease, forest fires, climate change, and so on. In order to identify strains that are suitable for the production of T. matsutake-inoculated seedlings, Pinus densiflora seedlings were inoculated with T. matsutake after in vitro rooting and mycorrhization was examined in the roots of T. matsutake-inoculated seedlings after 6 months. The mycorrhization rate was greater than 80% for 5 strains (NIFoS 421, 434, 1681, 1984, and 2001) out of 19 total strains. Seven strains (NIFoS 434, 441, 561, 562, 1016, 1807, and 1812) showed shoot/root ratios of less than 3.0 and had a seedling shoot biomass of 2.0 to 4.8 times higher than that of the root. Eight strains (NIFoS 441, 561, 562, 1016, 1807, 1812, 1984, and 2001) stimulated increases in shoot volume and three stains (NIFoS 441, 562, and 1812) promoted the growth of root biomass by mycorrhizal formation. In conclusion, 4 strains (NIFoS 434, 561, 1984, and 2001) out of 19 total strains tested showed higher mycorrhization rates and seedling growth than those of the other strains. We expect that the use of these four strains may contribute to T. matsutake-inoculated seedling production.

우리나라 산림에서 분리한 외생균근균의 균사생장에 있어 배지, 온도, pH의 영향과 셀룰라아제 활성

전성민 ( Sung Min Jeon ),김민수 ( Min Soo Kim ),가강현 ( Kaang Hyeon Ka ) 한국균학회 2012 韓國菌學會誌 Vol.40 No.4

우리나라 산림에서 수집한 외생균근균 8종 27개 균주에 대한 균사 생장 특성을 다양한 배양 조건(배지, 온도, pH)에서 조사하였다. 60일 배양 후, 모든 균주는 PDA와 MMN 배지에서 생장하였으나, 송이를 포함한 일부 균주들은 MEA나 SDA 배지에서 전혀 생장하지 않았다. 또한 대부분의 균주들을 PDA 배지에서 배양했을 때에 저온(10˚C)보다 고온(30˚C)에서 균사 생장이 저조하였다. 또한 PDA 배지 상에서의 최적 생장온도는 20-25˚C, PDB 배지 내 균사 생장을 위한 최적 pH는 4-5로 나타났다. Carboxymethyl cellulose(CMC) 활성은 모든 시험 균주에서 나타났으며, CMC(pH 5.0) 한천배지 상에서 최대의 셀룰라아제 활성을 나타낸 균주는 송이(1266)였다. Mycelial growth of ectomycorrhizal fungi (27 strains of 8 species) collected from Korean forests was observed on various culture conditions (media, temperature, pHs). After 60 days of incubation, all strains grown on potato dextrose agar (PDA) and modified Melin-Norkran`s agar (MMNA), whereas no mycelial growth was observed on malt extract agar (MEA) or sabouraud dextrose agar (SDA) in some strains including Tricholoma matsutake. Mycelial growth on PDA was poor at high temperature (30oC) than the low temperature (10oC). The optimal temperature on PDA and pH in potato dextrose broth (PDB) for mycelial growth in most strains were 20-25oC and pH 4-5, respectively. All strains tested showed the carboxymethyl cellulase (CM-cellulase) activity and the maximal cellullase activity was expressed by the mycelium of T. matsutake (KFRI 1266) on the CMC agar plate with pH 5.0.

주거래 은행의 자산건전성과 기업의 재무제표 비교가능성 간 관련성

전성민(Sung Min Jeon),이상혁(Sang Hyuk Lee) 한국경영학회 2023 經營學硏究 Vol.52 No.5

치매요양시설의 옥외공간 조성에 관한 기초연구

전성민 ( Sung Min Jeon ),권순정 ( Soon Jung Kwon ) 한국의료복지건축학회 2007 의료·복지 건축 Vol.13 No.1

한국에 자생하는 녹청균류의 기초 배양 특성

전성민 ( Sung-min Jeon ),가강현 ( Kang-hyeon Ka ),왕은진 ( Eun-jin Wang ),유림 ( Rhim Ryoo ),장영선 ( Yeongseon Jang ) 한국균학회 2018 韓國菌學會誌 Vol.46 No.2

Members of Chlorociboria are soft-rot ascomycetes that produce blue-green pigment. We investigated the growth characteristics of two Korean species of Chlorociboria, eight strains of Chlorociboria aeruginascens and Chlorociboria poutoensis, under various culture conditions (solid media, temperature, pH) and screened them for extracellular enzyme activity. Although the growth rate was slow, all tested strains of Chlorociboria spp. grew well on potato dextrose agar (PDA; 16.3~42.6 mm after 60 days) or Sabouraud dextrose agar (SDA), but not on malt extract agar (MEA). Compared with C. aeruginascens strains, C. poutoensis strains exhibited higher expression of blue-green pigments on both PDA and SDA media. The optimal temperature for mycelial growth was 20~25°C, and mycelial growth was lower at 30°C than at 10°C. All strains tended to have increased mycelial growth as the incubation temperature increased in the range of 10 to 20°C. The optimal pH of potato dextrose broth (PDB) for mycelial growth varied according to the strain under static culture conditions. Maximum biomass production was obtained at pH 6.0 for NIFoS 579 (114.3 ± 5.1 mg/60 days), but it maintained a stable pigment expression under a broad pH spectrum. The activities of both cellulase and laccase were observed in all tested strains of Chlorociboria spp. Enzyme activities of NIFoS 579 were remarkably higher than those of the other strains. From these results, we suggest that C. poutoensis NIFoS 579 is a potential candidate for use as a source of natural blue-green dye.