국내산 원유 중의 구리 및 망간 함량의 계절적 변화

곽병만,전연명,김강섭,이기웅,안장혁,장치훈,Kwak Byung-Man,Jun Yeun-Myoung,Kim Kang-Seob,Lee Ki-Woong,Ahn Jang-Hyuk,Chang Chi-Hoon 한국축산식품학회 2006 한국축산식품학회지 Vol.26 No.2

본 연구는 2003년 7월부터 2004년 6월까지 약 1년에 걸쳐 국내의 충청도 및 전라도지역에서 집유한 원유에 함유된 구리 및 망간의 함량을 계절별로 정량한 결과이다. 원유 중 구리와 망간 함량은 계절에 따른 변화가 크지 않았으며, 구리의 경우, 봄($3월{\sim}5윌$)은 $10.10{\sim}26.00{\mu}g/100 mL$로 평균 $13.79{\mu}g/100 mL$, 여름($6윌{\sim}8월$)은 $5.06{\sim}15.41{\mu}g/100 mL$로 평균 $10.17{\mu}g/100 mL$, 가을($9월{\sim}11월$)은 $5.04{\sim}19.70{\mu}g /100 mL$로 평균 $10.70{\mu}g/100 mL$, 겨울($12월{\sim}2월$)은 $6.96{\sim}17.80{\mu}g/100 mL$로 평균 $12.11{\mu}g/100 mL$로 나타났다. 한편 망간의 경우는 봄($3월{\sim}5월$)은 $3.00{\sim}8.30{\mu}g/100 mL$로 평균 $4.87{\mu}g/100mL$, 여름($6월{\sim}8월$)은 $2.30{\sim}6.44 {\mu}g/100mL$로 평균 $3.75{\mu}g/100 mL$, 가을($9월{\sim}11월$)은 $2.81{\sim}6.04{\mu}g/100mL$로 평균 $3.82{\mu}g/100 mL$, 겨울($12월{\sim}2월$)은 $2.25{\sim}9.02 {\mu}g/100 mL$로 평균 $4.48{\mu}g/100 mL$로 나타났다. 대체적으로 봄에 가장 높고 여름에 가장 낮은 경향을 보였으나, 계절에 따른 통계상의 유의적인 차이는 인정할 수 없었다. This study was conducted by using the ICP-AES (Inductively Coupled Plasma - Atomic Emission Spectrometry) to investigate the seasonal changes of copper and manganese content in the raw bovine milk. From July 2003 to June 2004, the milk samples were collected from the two geographical locations, Chungcheong-do and Jeolla-do, in Korean peninsula. Copper (Cu) content was determined in the range of min. 10.10 to max. 21.00 ${\mu}g$/100 mL (13.79 ${\mu}g$/100 mL) in spring, 5.06 to 15.41 (10.17) in summer, 5.04 to 19.70 (10.70) in autumn and 6.96 to 17.90 (12.11) in winter. For manganese (Mn), 3.00 to 8.30 (4.87) in spring, 2.30 to 6.44 (3.75) in summer, 2.81 to 6.04 (3.82) in autumn and 2.25 to 9.02 (4.48) in winter. Those data have shown that levels of copper and manganese was relatively constant but higher in Spring than other seasons, suggesting that the levels were not affected by seasons and different locations in Korea.

혈당 강하 요구르트 개발에 관한 연구

조영훈,신현정,장치훈,남명수,Cho Young-Roon,Shin Hyun-Jung,Chang Chi-Hoon,Nam Myoung-Soo 한국축산식품학회 2006 한국축산식품학회지 Vol.26 No.2

혈당 강하 요구르트의 개발을 위해 Streptococcus salivarius subsp. thermophilus와 Lactobacillus acidophilus145, bifido-bacterium infantis 혼합균주에 사균인 FK-23 유산구균, 피니톨, 식이섬유, 백강낭콩 추출 분말, 에리스리톨, 바나바 추출물 등의 기능성 성분을 포함한 요구르트를 제조하여 정상인과 혈당치가 높은 당뇨환자를 대상으로 간이 임상시험을 실시하였다. 1. 정상인의 식후 혈당치는 일반 요구르트를 섭취했을 때보다 혈당 강하 요구르트 음용 시 더 낮게 측정되었으여 임상대상자 대부분에서 동일한 현상이 나타났다. 2. 혈당치가 높은 당뇨환자 10명을 대상으로 식후 혈당치변화 및 음용기간 중 혈당치 변화를 확인한 결과 혈당 강하요구르트를 음용하기 전보다 음용 후 혈당치가 식후 30분, 식후 60분, 식후 120분 각각 19 mg/dL, 30 mg/dL, 31 mg/dL 감소하였다. 또한, 혈당 증가 억제율도 임상대상자의 80%가 감소하는 효과를 나타냈다. 3. 30일간 혈당 강하 요구르트를 음용한 후 식후 혈당치의 변화를 확인한 결과 음용전보다 음용 후 120분 경과 때, 혈당치가 59 mg/dL 감소하였으며 음용기간이 증가할수록 지속적으로 감소하는 경향을 나타내었다. This experiment was carried out to investigate the effect of hypoglycemic yogurt as a clinical test with person both who have diabetes and who don't have diabetes. Diabetes is a disease caused by unused and accumulated glucoses produced via the foods digestion due to the lack of insulin or lower tissue reaction to insulin. Patients with diabetes or complications of diabetes are on the rise annually. For the therapy and prevention of diabetes, a yogurt made from functional materials such as insulin-resistant FK-23 lactic acid bacteria, Pinitol, edible fiber which delays glucose-absorption, extract powder of glucose-absorption-inhibitory white bean and low-caloric Erythritol and banaba extract is effective for reduce or restrain the blood glucose levels. The blood glucose levels after a meal of non-diabetic persons were lower after drinking a hypoglycemic yogurt than they after drinking general yogurt. The blood glucose levels after a meal of 10 diabetic patients were checked after drinking the yoghurt. As a result, 19 mg/dL, 30 mg/dL, 31 mg/dL of blood glucose levels decreased in 30 min, 60 min and 120 min respectively after drinking the yogurt. Blood glucose inhibition rates of 80 % of the tested patients also decreased. The blood glucose level of the diabetic patients having drunk the yogurt for 30 days consistently decreased into 59 mg/dL in 120 min after a meal.

Static headspace, purge & trap 및 solid-phase microextraction을 이용한 시판우유의 휘발성 향기성분 분석

김성한(Sung-Han Kim),이홍민(Hong-min Lee),이기웅(Ki-Woong Lee),장치훈(Chi-Hoon Chang) 한국식품과학회 2006 한국식품과학회지 Vol.38 No.6

Column-switching HPLC를 이용한 성장기용 조제식 중 비타민 D<SUB>3</SUB>, K<SUB>1/SUB>의 동시분석

곽병만(Byung-Man Kwak),안장혁(Jang-Hyuk Ahn),장치훈(Chi-Hoon Chang) 한국식품과학회 2005 한국식품과학회지 Vol.37 No.6

HPLC-ELSDS 이용한 조제분유 중 fructooligosaccharides 및 raffinose 분석

신만섭(Man-Sub Shin),박재우(Jae-Woo Park),조미란(Mi-Ran Cho),송성옥(Sung-Ok Song),김춘선(Chun-Sun Kim),최춘배(Chun-Bae Choi),이승원(Scoung-Won Lee),이기웅(Ki-Woong Lee),장치훈(Chi-Hoon Chang),곽병만(Byung-Man Kwak) 한국식품과학회 2006 한국식품과학회지 Vol.38 No.6

Ciprofloxacin의 동물유래 병원세균에 대한 시험관내 항균효과시험

장경수,장치훈,김일택,박승춘,윤효인,전무형 충남대학교 수의과대학 동물의과학연구소 1996 動物醫科學硏究誌 Vol.4 No.-

In this study the antimicrobial spectrum and sensitivity of ciprofloxacin(CFX) against the major pathogenic bacteria isolated from the diseased poultry, pig, cattle in Korea were evaluated in comparison with morfloxacin(NFX), enrofloxacin(EFX), nalidixic acid(NA), gentamicin(GM), tetracycline(TC), erythromycin(EM), streptomycin(SM) and penicillin(PC). Increasing by paper disk diffusion test for total of 439 isolates from poultry, pig and cattle, CFX showed remarkably higher sensitivity(>83%) as compared with other drugs. When three quinolones such as CFX, NFX and NA were compared for the inhibition activity against 4 major pathogens, CFX induced significantly larger diameter of inhibition zone through all of the tested concentration than NFX and NA. As MICs of all drugs for 11 bacterial species isolated from poultry were measured, the MIC range of CFX was 0.125 - 40 ㎍/㎖ in Gram positives, ≤ 0.005 - 2.5 ㎍/㎖ in Gram negatives and 0.08 - 1.25 ㎍/㎖ with 12 isolates of Mycoplasma. MIC_50 and MIC_90 of CFX in these cases revealed consistently lower as compared with those of NFX, GM and PC. When the MICs of the drugs were tested with 11 bacterial species from the diseased pigs, MIC range of CFX was 0.05 - 3.5 ㎍/㎖ in Gram positives, ≤ 0.005 - 0.8 ㎍/㎖ with Gram negatives and 0.5 - 9.0 ㎍/㎖ with 11 isolates of Mycoplasma. MIC_50 and MIC_90 of CFX and NFX were remarkably lower than those of GM and PC. As MICs of CFX, NFX, GM and PC for 10 bacterial species isolated from cattle were tested, the MIC range of CFX was found 0.01 - 4.0 ㎍/㎖ in Gram positives, and ≤ 0.005 - 0.2 ㎍/㎖ in Gram negative bacteria. The MIC_50 and MIC_90 of CFX in cases of cattle appeared remarkably lower than those of the other drugs. CFX, as compared with the other drugs, showed invariably lower MIC_50 and MIC_90 in both of GRam positives and negatives. As MICs of CFX, NFX and PC for 12 reference bacteria were examined, MIC of CFX was 0.03 - 0.25 ㎍/㎖ that was much lower drug concentration than those of NFX(0.25 - 1.0 ㎍/㎖) and PC(0.5 - 16.0㎍/㎖).

忠南地域 屠畜豚의 肺病變으로부터 分離한 Actinobacillus pleuropneumoniae의 生物學的 및 免疫學的 特性

이종훈,장경수,장치훈,김일택,정용성,김귀현,전무형 충남대학교 수의과대학 동물의과학연구소 1996 動物醫科學硏究誌 Vol.4 No.-

An epidemiologic study on pleuropneumonia in the slaughter pigs(Chonan and Asan area, Chungnam province, Korea) during the period of January 1994 through December 1995 was conducted. Isolation of A. pleuropneumoniae was attempted in 425 pigs with pneumonic lesions. Biochemical properties, antimicrobial susceptibility, serotypes and pathogenicity of A. pleuropneumoniae isolates were investigated. In addition, outer membrane protein(OMP) of the isolates were extracted to determine its properties and immunogenicity in both mice and piglets. Of 3,395 slaughter pigs, pleuropneumonia was observed in 425 pigs(10.6%). A. pleuropneumoniae was isolated from 22 pigs(5.2%) out of 425 pigs with pneumonic lesions. The biochemical properties of all isolates were same as those of reference A. pleuropneumoniae strain. Among 22 isolates, 9, 1 and 12 isolates were serovar 2, 3 and 5, respectively. The results of antimicrobial susceptibility test revealed that the isolates showed high susceptibility to ciprofloxacin and cephalothin, moderate susceptibility to amikacin, gentamicin, kanamycin and streptomycin, and low susceptibility to erythromycin, tylosin and sulfadimethoxin. The isolates were varied in pathogenicity to mice. Median lethal dose of LE9402(serovar 2) and LE9511(serovar 5) were 9.2×10^7CFU and 2.8×10^7CFU, respectively. Specific pneumonic lesions were observed from the infected mice with clinical signs. Bacteria recovery rate was high in the lung, but low in heart blood and tracheas. Serovar 2 was found to be more pathogenic than serovar 5 in guinea pig. Mortality on guinea pigs inoculated with serovar 2(5.4×10^8 - 5.4×10^6CFU) abd serovar 5(2.8×10^8 - 2.8×10^6CFU) was 20-40% and 40-80%, respectively. A severe hemorrhagic lesions and focal pneumonic lesions were observed from dead guinea pigs. Bacteria recovery rate was relatively higher in the lung than that of other organs. In the SDS-PAGE analysis, OMP-enriched fractions of both isolates and reference strains contain common peptide bands equivalent to molecular weight of 17, 27, 42, 52 and 95Kd. In addition to common peptide bands, the bands which are specific to each isolate were also observed. The profiles of Sephadex G25 fractions showed 3 major peaks. The common peptide bands which were observed by SDS-PAGE of the crude OMPs were found i the peaks 1 and 2. The OMPs extracted from serovar 2(LE9402) and serovar 5(LE9511) provided high level of protection in mice(70-80%) and pigs(100%). All animals inoculated with OMPs were seroconverted, showing micro-agglutination titer of 640 to 1280.