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유방암세포주에서 고농도 5-fluorouracil의 세포주기 조절효과
장정순,양중일,장세호,이원섭,이종석,안명주,박병규,Jang, Joung Soon,Yang, Jung Ill,Chang, Seho,Lee, Won Sup,Lee, Jong Seok,Ahn, Myung-Ju,Park, Byung-Kiu 대한면역학회 2002 Immune Network Vol.2 No.1
Background: Chemotherapy with 5-fluorouracil (5-FU) has been one of the mainstay in breast cancer treatment. The effects of high dose 5-FU on cell cycle regulation were studied in breast caner cells. Methods: A breast cancer cell line MCF-7 was used. Protein expressions of G1/S cyclins, $p21^{Waf1/Cip1}$, cdk2, E2F1 and retinoblastoma were tested by western blot analysis. Immunoprecipitation and immune complex kinase assay were done for the assessment of E2F1/RB interacton and the activity of cdk2 respectively. Results: $p21^{Waf1/Cip1}$ expression was barely detectable in control cells. With addition of 5-FU level of $p21^{Waf1/Cip1}$ were induced and cyclin D3 level was decreased as cell growth decreases. In accordance with increased expression of $p21^{Waf1/Cip1}$, cyclin E-associated cdk2 kinase activity was reduced. Retinoblastoma protein (RB) became dephosphorylated and E2F-1 binding activity with RB was increased. Conclusion: In this situation of high concentration of 5-FU breast cancer cells tend to be G1/S cell cycle arrested. Overexpression of $p21^{Waf1/Cip1}$ and dephosphorylation of RB may mediate the effectss of 5-FU by inhibiting E2F-1 activity, which contributes to G1/S cell cycle arrest. These results could be an indicating landmark for further study of high dose chemotherapy with 5-FU.
장정순(Joung Soon Jang),전재범(Jae Bum Jun),이오영(Oh Young Lee),유대현(Dae Hyun Yoo),김성윤(Seong Yoon Kim),주경빈(Kyung Bin Joo) 대한내과학회 1990 대한내과학회지 Vol.39 No.3
N/A Dry mouth is a commnon symptom in rheumatoid arthritis patients and an important component of sjoren's syndrome. The symptoms of dry mouth are very sultle difficult to assess. So, different procedures have been employed to assess, alivary gland function. One of them, parotid sialography, is a useful diagnostic procedure for sialadenitis. But this kind of investigation can cause pain, swallowing of the parotid gland, or exceptional allergic reaction to radiologic contrast media. In addition, it is a relatively complicated procedure to implement. On the other hand, despite the fact that the salivary gland can readily be evaluated by ultrasonography (US), there have been few reports describing the value of US in salivary gland diseases. We investigated the discriminative role of US versus parotid sialography in 31 patients with complaints of dry mouth and took minor salivary gland biopsies prospectively. All the patients in our study were women, and the age distribution was 28 to 66. The results were as follows: 1) In parotid sialography, 16 of the 20 patients with sialadenitis in minor salivary gland biopsy had abnormal findings over stage 2, and nine of the 11 without sigladenitis had normal findings (p=0.003). 2) In parotid US, 15 of the 20 patients with sialadenitis in minor salivary gland biopsy had decreased, namely abnormal, parotid gland echogenecity, and eighe of the 11 without sialadenitis had normal findings (p=0.028). 3) Comparing paratid US with parotid sialography, a good correlation between US parotid gland echogenecity and sialographic abnormalities was found. Ten of the 13 patients with abnormal US findings had abnormal sialograms, whereas three of the 15 with normal parotid gland echogenecity had abnormal sialo-grams (p=0.003). 4) No significant difference in submandibular echogenecity on US could be demonstrated. ESR tends to increase for patients with decreased parotid gland echogenecity (p=0.03). In summary, our study demonstrated that US, although not perfectly sensitive or specific, is a valuable noninvasive imaging techinque in screening for salivary gland involvement in patients with complants of dry mouth. Together with other studies it could be used as a first step in the diagnostic investigation of sialadenitis.
강윤식,장정순,황영실,홍대용,김장락,Kang, Yune-Sik,Jang, Joung-Soon,Hwang, Young-Sil,Hong, Dae-Yong,Kim, Jang-Rak 대한예방의학회 2003 Journal of Preventive Medicine and Public Health Vol.36 No.3
Objective : This study was conducted in order to investigate predictors of smoking cessation in outpatients. Method : Subjects were 40f adult smoking patients who saw their doctors in the outpatient setting at a university hospital, regardless of their willingness of otherwise in smoking cessation. Physicians delivered a brief, stop smoking prompt to all patients who smoked one or more cigarettes a day. Then they referred to on-site counselors who provided a brief, nurse assisted intervention with a survey to a randomly assigned intervention group (200 smoking patients), whom the counselors telephoned later to prevent relapse or promote the motivation to quit, or gave only a survey to a control group (201 smoking patients). After at least 5 months, self-reported current smoking cessation was confirmed later using cut-off values of 7 ppm or less in expired alveolar air after breath holding portable CO analyzer. Results : After 5 months, subjects in the intervention group were 1.56 times (95% C.I. 0.89-2.73) more likely to quit smoking than those in the non-intervention group (14.0% vs. 9.0%). Willingness to quit smoking in a month, scheduled admission in a month, self efficacy score and FTND (Fagerstrom Test for Nicotine Dependence) score were all significantly related with smoking cessation. In stepwise multiple logistic regression, previous attempts to quit smoking were significant instead of self efficacy score. In the intervention group who had willingness to quit smoking in a month (132 smoking patients), FTND score, whether quit date was today, and whether quit promise paper was submitting were all significantly related with smoking cessation. In stepwise multiple logistic regression, scheduled admission in a month and whether quit date was today were significant predictor variables, Smoking cessation treatment should be tailored to individual smoking patients considering these predictors.
증례 : 혈액종양 ; 치료적 혈장반출술을 포함한 병합요법으로 치료된 후천성 혈우병 2예
박은경 ( Eun Kyung Park ),장정순 ( Joung Soon Jang ),박선양 ( Seon Yang Park ),이상재 ( Sang Jae Lee ) 대한내과학회 2009 대한내과학회지 Vol.77 No.3
Acquired hemophilia is a rare disorder associated with fatal bleeding caused by the development of autoantibodies against factor VIII. Here, we report the cases of two young women with acquired hemophilia who presented with massive internal hemorrhage and purpura. Both patients were successfully treated with combination therapy including factor VIII or factor VIII bypassing agent, immunosuppressants, and therapeutic plasmapheresis. (Korean J Med 77:367-370, 2009)
신령버섯균사체 액체배양물의 자가분해에 의한 항암성 isoflavone-conjugated glycoprotein 분리
김소영(So Young Kim),김영숙(Young Suk Kim),장정순(Joung Soon Jang),김보현(Boh Hyun Kim),라키브 압두르(Abdur Md. Rakib),김곤섭(Gon Sup Kim),김정옥(Jeong Ok Kim),하영래(Yeong Lae Ha) 한국생명과학회 2014 생명과학회지 Vol.24 No.12
신령버섯균사체(Agaricus blazei mycelia: ABM)를 대두박이 함유된 액체배지에 배양하고, 이것을 자가분해(53 ℃, pH 5.5, 120 rpm, 3 hr)하여 항암성이 강한 isoflavone-conjugated glycoprotein (Gluvone 이라 명명)을 분리하였다. Gluvone 은 지금까지 알려진 당단백질과는 달리 분자량이 작고(9,400 Da), isoflavone이 결합되어 있다는 점이 다르다, Gluvone 은 60% 탄수화물(glucose, fructose, ribose), 31% 단백질 및 2% isoflavone (daidzein, genistein)으로 구성되어 있었다. 이 Gluvone은 S-180 복수암세포, MCF-7 인체유선암세포에 대한 독성이 강하였고, S-180 세포로 유발한 mouse 복수암을 강하게 억제하였다. Most beta-glucans obtained from various fruit bodies of mushrooms and mushroom mycelial cultures have high-molecular weight glycoproteins, conjugated with beta-glucans. We report that isoflavone- conjugated glycolproteins (designated as gluvone) were isolated and exhibited stronger anticarcinogenic activities. Agaricus blazei mycelia (ABM) was cultured in a liquid medium containing soybean flakes for 14 days. The liquid culture was autolyzed by incubating at 53℃ (pH 5.5) for 3 h. A crude glycoprotein (CGP) fraction with a cytotoxic effect on a mouse ascite cancer cell line (S-180) and a human breast cancer cell line (MCF-7) was isolated from the autolyzed ABM cultures by 80% ethanol treatment. Gluvone was isolated from the CGP with Sephadex G-75 column chromatography. It exhibited a stronger anticancer effect than CGP against the S-180 cell-induced female ICR mouse ascites carcinogenesis. Gluvone with 9,400 daltons was identified as a glycoprotein conjugated with isoflavone. According to HPLC and GC analysis, in conjunction with ¹H-NMR spectral analysis, it contained 60% carbohydrates (glucose, fructose, and ribose), 31% protein, and 2% isoflavone (daidzein and genistein), which is a novel material. These results indicate that a strong anticarcinogenic gluvone was isolated from the autolyzed product of a submerged liquid culture of ABM, suggesting that autolysis could be a useful tool to produce antitumor agents.