마우스에서 확립된 타액선 동위종양에서 혈관성 전이관련 인자의 발현

장재현,권광준,박영욱,Jang, Jae-Hyun,Kwon, Kwang-Jun,Park, Young-Wook 대한악안면성형재건외과학회 2007 Maxillofacial Plastic Reconstructive Surgery Vol.29 No.6

Background and Purpose: Some subtypes of malignant salivary gland tumors such as adenoid cystic carcinoma (ACC) frequently result in distant metastasis of vascular origin, which are main causes of treatment failure. The reasons for the affinity for vascular metastatic potential are unclear. Therefore, molecular characteristics that influence the dissemination of metastatic tumor cells are important for the design of more effective treatment of salivary ACC. Tumor angiogenesis has been known to be essential for the distant metastasis of malignant cells. So, we determined expressions of vascular metastasis related factors in orthotopic (parotid) murine models of parotid ACC and compared with those in ectopic (subcutis) tumors of athymic mice. Experimental Design: Using specimens from murine parotid (orthotopic, experimental group) and subcutaneous (ectopic, control group) tumors, which have developed via transplantation of tumor cells, originated from human parotid ACC, we performed immunohistochemical assays with anti-vascular endothelial growth factor (VEGF), basic fibroblast growth factor (bFGF, FGF2), matrix metalloproteinase (MMP)-9, and interleukin (IL)-8 antibodies. We also performed immunohistochemical assays with VEGF receptor (VEGFR)-1, VEGFR-2, VEGFR-3, and phosphorylated VEGFR-2. Results: Transplantation of human ACC tumor cell $(5{\times}10^5)$ into the parotid and subcutis successfully resulted in orthotopic (parotid) and ectopic (subcutaneous) tumors in athymic mice. Immunohistochemical staining demonstrated higher expression of major angiogenic factors (VEGF, bFGF, MMP-9) in the orthotopic tumors than in ectopic tumors (P<0.05). But the expression level of angiogenic receptors were same in orthotopic and ectopic tumors of parotid ACC. Conclusion: VEGF, bFGF, and MMP-9 could be a good candidates for antiangiogenic therapy for the contol of vascular metastatic lesions of salivary ACC.

확산 반사판 표면 구조에 따른 광학적 특성에 관한 연구

장재현(Jae-Hyun Jang),박광일(Kwang-Il Park),김종태(Jong-Tae Kim),유영문(Young Moon Yu) 한국조명·전기설비학회 2017 조명·전기설비학회논문지 Vol.31 No.3

In this paper, the relationship between the optical properties and the surface structure of the diffuse reflector used in the LED lighting fixtures was studied. Ten samples with different levels of the surface roughness and surface gloss characteristics were prepared using the sand blast method. Measured surface roughness represented by average Ra was widely distributed from 3.34 to 22.59㎛. The surface gloss characteristics of sample were influenced by the frequency of period of the measured roughness. Reflectance for each wavelength (360∼740nm) was same within 1% of deviation and total reflectance was 88% within 0.12% of deviation regardless of surface roughness and surface gloss characteristics. Scattering characteristics were analyzed by BSDF(Bidirectional scattering distribution function) measurement system at the incident angles from 0 to 60° and wavelengths of 645nm and 530nm to analyze the dependency of scattering characteristics on the surface roughness and gloss characteristics of samples. The higher the roughness, the higher FWHM value was regardless of the surface gloss characteristics. At the same condition of roughness, the FWHM value of the matt type sample is higher than that of the glossy type sample with matte type. The deviation of the FWHM value between the sample with matt type and the one with glossy type was smaller as the roughness increased. There was not so much difference in scattering effect on wavelength of incident beam depending on the surface of roughness and gloss characteristics.

한국인에서 염증성 장질환 급성 악화의 계절적 변화

장재현 ( Jae Hyun Jang ),이수희 ( Su Hee Lee ),성준모 ( Jun Mo Sung ),박정호 ( Jung Ho Park ),김홍주 ( Hong Joo Kim ),조용균 ( Yong Kyun Cho ),손정일 ( Chong Il Sohn ),전우규 ( Woo Kyu Jeon ),김병익 ( Byung Ik Kim ),박동일 ( Dong 대한장연구학회 2012 Intestinal Research Vol.10 No.2

Background/Aims: Conflicting data have been reported about the seasonal variation in flare-up of inflammatory bowel disease (IBD). The aim of this study was to assess the occurrence of seasonal variations in flare-up of IBD in the Korean population. Methods: Patients with IBD, who underwent more than 1 year of follow-up and showed more than 1 episode of flare-up, were included. Flares of disease were refined as follows; receipt of a new prescription and increasing dose of corticosteroids, 5-ASA or immunosuppressant; hospitalized or operated due to development and worsening of symptoms; elevation of inflammatory marker due to worsening of Crohn`s Disease Activity Index (CDAI) or Mayo score. In addition, for patients with more than two episodes of flare-up, they were investigated in a monthly and seasonal pattern. Results: Of 573 patients with IBD, 46 patients with Crohn`s disease and 61 patients with ulcerative colitis were enrolled. There was no association between month of the year and flare of Crohn`s disease (P=0.06) or ulcerative colitis (P=0.58). Further, no association between season of the year and flare of Crohn`s disease (P=0.06) or ulcerative colitis (P=0.68). However, in Crohn`s disease who experience 2 or more flare-ups, symptoms occurred more frequently during winter, especially December (P=0.029). Conclusions: In the Korean population, there was no association of seasonal and monthly variation in flares of Crohn`s disease and ulcerative colitis. However, in Crohn`s disease, individuals with 2 flar-up or more, symptoms occurred more frequently during December. (Intest Res 2012;10:161-167)

체외 배양된 SNU-1 세포주에서 transglutaminase C antisense inhibition이 일으키는 세포핵질 변화

장재현(Jae-Hyun Jang),이석근(Suk-Keun Lee),박영욱(Young-Wook Park) 대한구강악안면외과학회 2003 대한구강악안면외과학회지 Vol.29 No.2

It has been known that transglutaminase C (TGase C, TGase II) is directly participated in the DNA organization of chromosome, and affects the cellular processes such as proliferation, differentiation, and apoptosis of cells, but still not known what mechanism is working on. In this study, the cytogenetic and the immunohistochemical methods were used to observe the TGase C expression in the nuclear chromosome of the proliferating cells, especially in mitotic stage. The human gastric adenocarcinoma (SNU-1) cell line was used for immunohistochemistry and antisense inhibition study in vitro. The present study was also aimed to disclose the efficiency of antisense inhibition by using antisense oligonucleotide DNA labeled with fluorescence, and found that anti-TGase C probe was diffusely infiltrated into the cytoplasm and the nucleus of the cell. By the antisense inhibition the nuclei of SNU-1 cells became rough nuclear shape, as they were greatly reduced in TGase C immunoreactivity both for the normal and apoptotic SNU-1 cells. However, it is clearly presumed that the TGase C directly interacts with the chromosome of SNU-1 cells and it may play an important role in the division and organization of the chromosome during the mitotic stage.

Single cell-strain부터 유래된 무세포 효소 시스템을 이용한 톨루엔 및 아세트산 분해

장재현 ( Jae Hyun Jang ),김예지 ( Yeji Kim ),노태용 ( Tae Yong Roh ),박중곤 ( Joong Kon Park ) 한국화학공학회 2016 Korean Chemical Engineering Research(HWAHAK KONGHA Vol.54 No.5

본 연구에서는 톨루엔 분해 균주인 Pseudomonas putida와 아세트산 분해 균주인 Cupriavidus necator에 무세포 효소 시스템(cell-free enzyme system)을 적용하여 톨루엔과 아세트산에 대한 분해 가능성을 확인하는 실험을 수행하였다. P. putida는 톨루엔 존재 하에서만 toluene dioxygenase를 생성하여 톨루엔을 cis-toluene dihydrodiol로 분해하며, C. necator는 acetyl coenzyme A synthetase-1을 생성하여 아세트산을 acetyl CoA로 전환시켜 생존에 필요한 ATP나 생분해성(biodegradable) 고분자인 Polyhydroxyalkanoate (PHA)를 합성한다. P. putida의 톨루엔 분해 효소인 toluene dioxygenase는 유도효소이기 때문에 toluene dioxygenase 생성 전과 후로 나누어 실험을 진행하였다. P. putida의 톨루엔 분해능력 확인을 위한 gas chromatography (GC) 분석 결과, 대조군과 toluene dioxygenase 생성 전인 실험군 1에서는 검출된 톨루엔의 양이 거의 유사하였으나, toluene dioxygenase 생성 후인 실험군 2에서는 검출된 톨루엔의 양이 대조군 및 실험군 1에 비해 감소하였다. 또한 C. necator의 아세트산 분해능력 확인을 위한 gas chromatography-mass spectrometer (GC-MS) 분석 결과, 무세포 효소 시스템을 적용한 실험군에서는 아세트산에 대한 피크가 검출되지 않았다. 따라서 P. putida와 C. necator는 무세포 효소 시스템 적용 후에도 톨루엔 및 아세트산 분해 능력이 유지되었으나, P. putida는 무세포 효소 시스템을 적용하기 전에 유도 효소를 생성하는 과정이 필요하다. This study deals with the possible degradation of toluene and acetic acid when subjected to cell free enzyme system from the toluene degrading bacteria Pseudomonas putida and acetic acid degrading bacteria Cupriavidus necator. P. putida produces toluene dioxygenase only under the existence of toluene in culture medium and toluene is degraded to cis-toluene dihydrodiol by this enzyme. C. necator produces acetyl coenzyme A synthetase-1 and converts acetic acid to acetyl CoA in order to synthesize ATP to need for growth or PHA which is biodegradable polymer. In case of toluene degradation, the experiment was conducted before and after production of toluene dioxygenase as this enzyme, produced by P. putida, is an inducible enzyme. Toluene was detected using gas chromatography (GC). Similar amount of toluene was found in control group and before production of toluene dioxygenase (experimental group 1). However, reduction in toluene was detected after the production of toluene dioxygenase (experimental group 2). Acetic acid was detected through application of gas chromatography-mass spectrometer (GC-MS). The results showed the acetic acid peak was not detected in the experimental group to apply cell-free enzyme system. These results show that the cell-free enzyme system obtained from P. putida and C. necator retained the ability to degrade toluene and acetic acid. However, P. putida needs to produce the inducible enzyme before preparation of the cell free enzyme system.

증례 : 닭 골편에 의해 발생한 간농양 1예

장재현 ( Jae Hyun Jang ),원경준 ( Kyung Jun Won ),이준 ( Jun Lee ),변유미 ( Yu Mi Byeon ),박찬국 ( Chan Guk Park ),김만우 ( Man Woo Kim ) 대한내과학회 2007 대한내과학회지 Vol.73 No.1S

백서에서 레이저 조사가 연조직 치유에 미치는 영향에 관한 연구

박영욱,장재현,김정환,박정민,이석근,Park, Young-Wook,Jang, Jae-Hyun,Kim, Jung-Hwan,Park, Jung-Min,Lee, Suk-Keun 대한악안면성형재건외과학회 2009 Maxillofacial Plastic Reconstructive Surgery Vol.31 No.3

Objectives: This study is aimed to compare the wound healing processes between conventional scapel wound and Er,Cr:YSGG (Erbium,Chromium, Yttrium, Scandium, Gallium, Garnet) laser wound using experimental animals. Experimental Design: Two types of wounds were made by linear and round incisions using scalpel and Er,Cr:YSGG laser, respectively, on the thigh of Sprague-Dawley rats. Sprague-Dawley rats were serially sacrified as follows: post operative 12, 24, 48 hours, and 3, 7, 14 days. The skin wounds were grossly and microscopically analyzed during the healing period. Result: The Er,Cr:YSGG laser incision showed better wound healing for the linear incision experiment than the scapel incision. Whereas the scapel incision showed better wound healing for the round incision experiment than the Er,Cr:YSGG linear incision. As the Er,Cr:YSGG laser damage in the round incision experiment could be much increased compared with the round incision by scapel. So, the round incisions by the Er,Cr:YSGG laser were resulted in the poor wound healing compared with those by the scapel. Conclusion: The Er,Cr:YSGG laser is more favorable for the fast linear incision, while the scapel is more favorable for the modified round incision.

만성 하악골 골수염에서 발생한 편평상피세포암종

박영욱,박정민,장재현,김지혁,권광준,이석근,Park, Young-Wook,Park, Jung-Min,Jang, Jae-Hyun,Kim, Ji-Hyuck,Kwon, Kwang-Jun,Lee, Suk-Keun 대한악안면성형재건외과학회 2008 Maxillofacial Plastic Reconstructive Surgery Vol.30 No.5

We experienced a rare case of oral squamous cell carcinoma arisen from gingival tissues overlying prolonged chronic osteomyelitis of the mandible. A 66 years old man complained of unhealed extraction sockets of left mandibular second premolar and first molar, and showed extensive leukoplakia in the gingival tissues of the same area. The inflammation of the socket granuloma became severe and extended into adjacent mandibular proper, resulted in diffuse suppurative chronic osteomyelitis of mandibular body, exhibiting irregular osteolytic changes of mandibular trabecular patterns in mottled radiolucent appearance. The leukoplakia was initially diagnosed under microscope, and the involved gingival tissues were radically removed. Thereafter, the gingival soft tissue inflammation involving the mandibular osteomyelitis was hardly healed for two years. During the period of repeated surgical treatments for the inflamed lesion, nine biopsies were taken sequentially. Until the eighth biopsy, there consistently showed the suppurative osteomyelitis with ingrowing gingival tissues into the bony inflammatory lesion. The gingival epithelium showed the features of leukoplakia but no evidence of malignant changes. However, the ninth biopsy, taken about 2 years after initial diagnosis, showed the early carcinomatous changes of the gingival epithelium. The neoplastic epithelial cells were relatively well differentiated with many keratin pearls, and infiltrated only into underlying connective tissues. So, we presumed that the present case of squamous cell carcinoma was caused by the persistent inflammatory condition of the mandibular osteomyelitis, and also suggest that the leukoplakia should be carefully removed in the beginning to prevent the neoplatic promotion of the chronic inflammation.

구강 내 소수술에 있어 Midazolam을 이용한 의식하진정 치료원칙

김성민,김지혁,박영욱,장재현,권광준,김세정,남대우,권기열,Kim, Soung-Min,Kim, Ji-Hyuck,Park, Young-Wook,Jang, Jae-Hyun,Kwon, Kwang-Jun,Kim, Se-Jung,Nam, Dae-Woo,Kwon, Ki-Yeul 대한치과마취과학회 2003 Journal of Dental Anesthesia and Pain Medicine Vol.3 No.1

Background: Conscious sedation is a minimally depressed level of consciousness that retains the patient's ability to maintain the airway continuously and to respond appropriately to physical stimulation and verbal command at any time, produced by a pharmacologic or nonpharmacologic method or a combination thereof. In dental anesthesia, a state of sedation in which the conscious patient is rendered free of fear, apprehension, and anxiety through the use of pharmacologic agents. Midazolam is a useful drug of benzodiazepine on minor operation of dentistry. The purpose of this study is to estimate the appropriate dosage protocol of midazolam to the patient receiving intravenous sedation at the department of oral and maxillofacial surgery. Methods: Total subject was fifty-one patients and fifteen patients of this subject were decided to control group (Group I) that they were not received intravenous sedation. The experimental group was randomly subdivided into two groups and each of those groups was administrated to 0.05 mg/kg (Group II) and 0.08 mg/kg (Group III) each other. All patients of the subject were surveyed with postoperative one day questionaire to evaluate the level of sedation. Results: In view of antegrade amnesia, 1. Group II and III were statistically different compared to Group 1, respectively. 2. Group II and III were not statistically different. Conclusions: Apparent antegrade amnesia was found in both groups 0.05 mg/kg and 0.08 mg/kg intravenously administered. So we present the protocol of the department of oval and maxillofacial surgery, Kangnung National University Dental Hospital. But further studies concerning patient's age, body weight, and general condition will be carried out.

아이스링크 빙판 내부 LED디스플레이 결빙 구조 및 영상 최적화에 관한 연구

박광일(Kwang Il Park),장재현(Jae-Hyun Jang),유영문(Yong Moon Yu) 한국조명·전기설비학회 2018 조명·전기설비학회논문지 Vol.32 No.3

In this paper, we investigated the structure of ice for optimized image mapping after installing LED display device in the ice. The LED display device installed inside the ice transmits the image to the outside of the ice sheet, while the projector above the ice reflects the image from the ice surface. The core part that affects the LED display and projector image inside the ice structure is the scattering layer. The light transmittance was measured by the scattering layer application frequency, and the relative luminance ratio was calculated by measuring the LED display transmission luminance and the projector reflection luminance. Then, the mapping characteristics that overlapped the LED display image and the projector image were observed and the optimum image mapping condition was confirmed. Through this study, it was confirmed that the larger the scattering layer concentration, the smaller the difference between the LED display luminance and the projector luminance, and the better the mapping image can be obtained.