Staphylococcus aureus와 Bacillus cereus에 대한 유산과 과산화수소의 증식억제 효과

장재선,고종명,김용희,Jang Jae Seon,Go Jong Myeong,Kim Yong Hee 한국환경보건학회 2005 한국환경보건학회지 Vol.31 No.2

This study was performed to investigate inhibitory effect on growth of Staphylococcus aureus and Bacillus cereus in lactic acid, hydrogen peroxide and combination of lactic acid and hydrogen peroxide. The minimun inhibitory concentration (MIC) of lactic acid in Staphylococcus aureus were 2500 ppm at pH 7.0, 1250 ppm at pH 5.5, 6.0 and 6.5, while in Bacillus cereus 625 ppm at pH 5.5 and 6.0, 1250 ppm at pH 6.5 and 7.0, respectively. MICs of hydrogen peroxide in Staphylococcus aureus were 50 ppm at pH 6.0, 75 ppm at pH 6.5 and 7.0, while in Bacillus cereus was 75 ppm at pH 5.0, 5.5 and 6.0, respectively. MICs of combined treatment of lactic acid and hydrogen peroxide in Staphylococcus aureus were 1250 ppm of lactic acid with 25 ppm of hydrogen peroxide and 625 ppm of lactic acid with 50 ppm of hydrogen peroxide. When Bacillus cereus were with 1250 ppm of lactic acid with 50 ppm of hydrogen per-oxide and 625 ppm of lactic acid with 75 ppm of hydrogen peroxide at 6.5. The correlations between MICs of lactic acid and hydrogen peroxide in S. aureus and B. cereus obtained through the coefficient of determination ($R^2$). $R^2$ value were 0.9934 and 0.9986, respectively. The inhibitory effect of lactic acid and hydrogen peroxide in S. aureus and B. cereus could be confirmed from the result of this experiment.

경기지역 중국인 유학생의 스트레스가 식습관 및 식품구매 형태에 미치는 영향

장재선,Jang, Jae-Seon 한국식품영양학회 2019 韓國食品營養學會誌 Vol.32 No.3

This study was performed to provide fundamental data on the dietary habits and food purchase types according to the stress patterns. The subject was 312 Chinese students in Gyeonggi region through a self-administered questionnaire. According to the results of the reliability analysis, the stress items showed an average of 2.91 and 0.846 for Cronbach alpha coefficient. The results of ANOVA analysis on the difference of dietary habits according to stress are as follows; There were significant differences for the meal frequency per day according to schoolwork, economic, general living, and dietary habits (p<0.05). Also, the general living pattern showed significant differences for the meal outside frequency and Chinese food intake (p<0.05). The results of ANOVA analysis on the difference of food purchase type according to stress are as follows; There was significant differences in degree of use of convenience foods according to interpersonal relationship, cooking method of convenience foods according to economics, the selection criteria of convenience foods according to schoolwork (p<0.05). The correlation coefficient of dietary habits and convenience food intake are 0.223, -0.147 in stress degree and dietary habits. In conclusion, I would like to provide the basic data necessary for the right choice of Chinese students' dietary habits and food purchases.

유기산이 Escherichia coli O157:H7, Salmonella 및 Listeria monocytogenes의 증식에 미치는 영향

장재선,이혜정,오보영,이제만,고종명,김용희,Jang, Jae-Seon,Lee, Hye-Jeong,Oh, Bo-Young,Lee, Jea-Mann,Go, Jong-Myeong,Kim, Yong-Hee 한국환경보건학회 2007 한국환경보건학회지 Vol.33 No.5

The inhibitory effect of the food processing agent on growth of Escherichia coli O157:H7, Salmonella Enteritidis, and Listeria monocytogenes was performed with organic acid, and combination of citric acid, acetic acid, propionic acid and vanillic acid. The minimun inhibitory concentration(MIC) of propionic acid was 5,000 ppm in E. coli O157:H7, 2,500 ppm in Salmonella Enteritidis and Listeria monocytogenes. MIC of citric acid was 10,000 ppm in E. coli O157:H7 and Salmonella Enteritidis, 2,500 ppm in Listeria monocytogenes. MIC of acetic acid was 2,500ppm, while in vanillic acid was 5,000 ppm in Escherichia coli O157:H7, Salmonella Enteritidis, and Listeria monocytogenes. MIC of combined organc acid in E. coli O157:H7 were 2,500ppm in PC, 1,250 ppm in PA, PV, CA, CV and AV. MIC of combined organc acid in Salmonella Enteritidis were 2,500 ppm in PC, PA, PV, CA, and CV, 1,250 ppm in AV. MIC of combined organc acid in Listeria monocytogenes were 1,250 ppm in all treatment group. MIC of combined treatment of three organc acid in E. coli O157:H7, S. Enteritidis and L. monocytogenes were 1,250 ppm in PCA, PCV, PAV and CAV. The inhibitory effect of organc acid in E. coli O157:H7, S. Enteritidis and L. monocytogenes could be confirmed from the result of this experiment. Therefore, it was expected that the food process would increase or maintain by using organic acid.

최근 5년간 인천지역 설사환자에서 분리한 Salmonella Enteritidis와 Salmonella Typhimurium의 항생제 내성 양상

장재선,이제만,공용우,이미연,Jang, Jae-Seon,Lee, Jea-Man,Gong, Young-Woo,Lee, Mi-Yeon 한국환경보건학회 2013 한국환경보건학회지 Vol.39 No.3

Objectives: Antimicrobial resistance and multidrug resistance patterns have been studied with a total of 189 samples of Salmonella Enteritidis and Salmonella Typhimurium isolated from diarrhea patients in Incheon from 2008 to 2012. Methods: Antimicrobial resistance tests were determined by Disc Diffusion method. Results: The serological distribution of Salmonella spp. showed 108 strains (30.1%) of S. Enteritidis, 81 strains (22.6%) of S. Typhimirium, eight strains (8.0%) of S. Typhi, 11 strains ( 3.1% ) of S. Paratyphi, and the 151 other strains (42.1%). The separation rate of Salmonella spp. by year showed 14.5% (52 strains) in 2008, 13.6% (49 strains) in 2009, 22.8% (82 strains) in 2010, 25.3% (91 strains) in 2011, and 23.7% (85 strains) in 2012. Additionally, the separation rate of S. Enteritidis and S. Typhimirium in 2010 was the highest. The Salmonella spp. isolated from diarrhea patients showed significant differences according to age (p<0.05), gender (p<0.01) and medical institution (p<0.05). The highest resistance was found to the following antimicrobial agents: imipenem 77 strains, ampicillin 47 strains, ciprofloxacin 34 strains, nalidixic acid 29 strains for S. Enteritidis, and ampicillin 45 strains, nalidixic acid 45 strains for S. Typhimurium. Separated S. Enteritidis and S. Typhimurium resistance to the antibiotics by the year showed significant differences (p<0.05). The patterns of multidrug resistance rates were 43.1% (47 strains) for one drug, 8.3% (9 strains) for two drugs, 11.0% (12 strains) for three drugs, 15.62% (17 strains) for four drugs, and 13.7% (15 strains) for five or more drugs for S. Enteritidis. For S. Tyhpimurium, the rates were 15.0% (12 strains) for one drug, 10.0% (8 strains) for two drugs, 6.3% (five strains) for three drugs, 18.7% (15 strains) for four drugs, and 23.8% (19 strains) for five or more drugs. Conclusion: The antibiotic resistance issue is directly related to people's lives. Thus, the usage of antibiotics should be reduced in order to manage antibiotic resistance.

전공 여부에 따른 대학생의 유전자재조합식품의 인식, 지식 및 구매 태도에 관한 비교 연구

장재선,Jang, Jae Seon 한국식품영양학회 2016 韓國食品營養學會誌 Vol.29 No.1

This study was analyzed to provide fundamental data of food safety education way on the recognition, knowledge and purchase attitude of GMO foods. Data was collected from 270 University students in the Gyeong-gi region through a self-administered questionnaire. According to the results of the reliability and factor analysis, the knowledge and purchase attitude of GMO foods showed 0.742 and 0.832 in Cronbach alpha coefficient, 0.791 and 0.831 in KMO. There were significant differences in the recognition and intake possibility and purchase will, safety and absorption to a GMO foods between the specialty person and non-specialist (p<0.05). By the kind of GMO foods it appeared highly by the order corn (70.0%), tomato (67.5%), beans (63.3%) and potato (51.3%). There were significant differences in the general knowledge of GMO foods (p<0.05), whereas no significant differences in question 'There were no genes in ordinary beans' by a presence of a specialty (p>0.05). After the purchase attitude difference to the GMO foods was analyzed by a presence of a specialty, I appeared most highly in the question to which I say 'I think a GMO foods is ethical.' by a negative question and 'I think a GMO foods is mass-produced, and it's possible to make a food problem decrease.' by an affirmative question. Therefore, the purchase attitude of GMO foods showed a significant differences of University students according to their major (p<0.05). Based on this study, it is considered that provision of detailed and continuous education must be accomplished by raising the recognition of GMO foods and its acceptance level, allowing a proper understanding of GMO foods to be conveyed and assisting subjects from receiving the information they desire effectively by various education mediums.

인천지역 주민들의 건강관련 생활습관과 식품선호도 및 체성분의 비교 연구

장재선,Jang, Jae-Seon 한국식품영양학회 2015 韓國食品營養學會誌 Vol.28 No.4

The present study was designed to investigate health-related life habits, food preference, body composition for proper dietary habits, health promotion of communities in Incheon area. The effects of personal characteristics and health-related life habits such as gender, age, having breakfast, smoking, drinking, and exercise were analyzed using a surveying. 961 community subjects (262 males and 699 females) were investigated using a questionnaire and Inbody. Data were analyzed by descriptive statistics, chi squared test, and one-way ANOVA (analysis of variation) with SPSS/WIN 21.0. The result of gender distribution showed there were 262 males and 699 females, and with respect to the effect of gender on health-related life habits, smoking, drinking, and exercise showed significant differences (p < 0.05), whereas having breakfast was not significantly difference (p>0.05). Therefore, the present provide evidence of a relationship between health-related life habits and gender. Regarding the effect of BMI on health-related life habits, exercise showed significant differences (p < 0.05), whereas smoking, drinking, and having breakfast were not significantly difference (p>0.05). Thus, the present study also provides evidence of a relationship between health-related life habits and BMI. Our analysis shows that food preference and body composition were significant different from health-related life habits such as smoking, drinking, exercise, and having breakfast (p < 0.05). Our analysis showed that body composition was significant differences from health related habits such as smoking, drinking, exercise, and breakfast (p < 0.05). In conclusion, the present study suggests that diet guidelines support and improve health promotion designed by communities.

일부 노인들의 건강관련 생활습관, 식습관, 영양지식 및 식품섭취에 관한 비교 연구

장재선,홍명선,Jang, Jae-Seon,Hong, Myung-Sun 한국식품영양학회 2015 韓國食品營養學會誌 Vol.28 No.6

This study was designed to provide the basic data of an effective nutrition education for desirable lifestyle and dietary habits to improve the nutritional status for the elderly by investigating health-related lifestyle, dietary habits, nutritional knowledge, and food intake of the elderly. The subjects included 58 elderly men and 146 elderly women. 35.8% had no more than an elementary school education. Most subjects (54.4%) were widows/widowers. Most subjects (71.0%) made over 150,000 won. 52.5% of subjects lived in houses. Smoking, drinking, exercise, the average sleeping time were significantly difference between the gender (p<0.05). In terms of dietary habits, our results showed that 80.9% of respondent eat regularly meals, including breakfast (83.5%). Both sexes prefer soft and salty food. Women enjoys more spicy and salty food compared with men and then shows meaningful difference (p<0.05). Their favorite meals are soup, stew, salad and boiled vegetables. Compared to women, men have a higher rate of correct answers about questions related to nutrition knowledge as showing significantly difference (p<0.05). In a study of dietary habits, they take in carbohydrates with the highest percentage and following by vegetables and fruits. The amount of meat, first and egg they eat is more than the previously. Eating meat is higher men as showing significant difference (p<0.05). In summary, nutritional status for the elderly shows healthy lifestyle and diet about half of those and most them try to live healthy life in future. It is hope that the elderly may need to learn proper nutrition knowledge for healthy lifestyle and nutrition education and counseling for building up healthy lifestyle and desirable dietary habits. Furthermore, it is necessary to start work to establish a baseline nutritious evaluation for the elderly and at a time to study the development of standard eating tool proven reliability and validity, consequently to provide a basic framework for the evaluation of nutritional status.

당귀, 작약, 천궁, 삼백초, 인진쑥 및 산조인을 첨가한 한방차의 팽화 온도별 고형분 용출율과 Benzo[${\alpha}$]pyrene의 변화

장재선,최미용,오성천,Jang, Jae-Seon,Choi, Mi-Yong,Oh, Sung-Cheon 한국응용과학기술학회 2014 한국응용과학기술학회지 Vol.31 No.2

팽화 처리온도를 각각 $140{\sim}220^{\circ}C$로 한 한방차 제품의 성분변화를 분석한 결과는 다음과 같다. 처리온도의 상승에 따라 일부 탄화가 발생하며 조회분 함량이 상대적으로 상승하는 소폭의 변화가 있었고, 조단백질 및 조지방 함량은 거의 변화가 없는 것으로 나타났으며 수분함량은 감소하였다. 한방차의 고형분 용출률은 0.18~0.27%(w/w)로 나타내었는데, 팽화온도가 상승할수록 증가하였다. 고형분의 용출은 온도가 화학적 변화보다 물리적 변화에 의해 식품의 원재료 성분인 탄수화물, 단백질, 지방 등이 천연 상태에서 상호가교 결합이 물리적인 힘으로 어느 정도 파괴되어 성분의 용출이 용이해지기 때문인 것으로 생각된다. 벤조피렌 함량은 0.18~0.24ppb로 처리온도, 원재료에 따라 B(${\alpha}$)P 함량에 차이가 발생한 것으로 나타났다. The following study is the result of herbal teas puffed at different temperatures between $140{\sim}220^{\circ}C$. There was change of single breadth that some carbonization occurs according to rise of processing temperature and crude ash content rises relatively, and crude protein and crude fat content had hardly changed and moisture content decreased. The solid elution rate of the herbal teas appeared by 0.18~0.27% (w/w), it increased as puffing temperature rises. The reason for the increase in solid elution rates is due to the breakage of cross bridges between the raw materials in the herbal tea which are carbohydrates, proteins, lipids and etc. after treatments of physical changes rather than chemical ones. Benzopyrene content happened difference in B(${\alpha}$)P content according to processing temperature, raw material by 0.18~0.24 ppbs.

로스팅 조건이 삼백초, 인진쑥, 산조인, 당귀, 작약 및 천궁을 첨가한 한방차의 벤조피렌 변화에 미치는 영향

장재선,최정윤,오성천,Jang, Jae-Seon,Choi, Jeong-Yun,Oh, Sung-Cheon 한국응용과학기술학회 2014 한국응용과학기술학회지 Vol.31 No.4

한방차의 roasting 온도를 $80{\sim}140^{\circ}C$ 범위로 하여 성분 변화를 분석한 결과, 온도의 상승에 따라 수분함량이 감소하고 일부 탄화가 발생하며 조 회분 함량이 소폭 상승하였고, 조 지방 및 조단백질 함량은 소폭 감소하였다. 한방차의 고형분 용출율은 0.15~0.32%(w/w)를 나타내었는데, roasting 온도가 상승할수록 감소하는 경향을 나타내었다. 처리온도가 $80{\sim}110^{\circ}C$구간에서는 큰 변화를 나타내지 않은 반면 $110{\sim}140^{\circ}C$구간에서는 고형분 용출율이 급격히 감소하였다. 온도가 상승할수록 용출율이 감소하는 것은 내부 조직이 치밀하여 상대적으로 용출이 어렵기 때문이다. 벤조피렌 함량은 0.29~0.51ppb으로 온도가 높을수록 $B({\alpha})P$함량이 증가하였다. 처리온도와 원재료에 따라 $B({\alpha})P$ 함량에 차이가 발생하며, 내부온도는 약 $200^{\circ}C$정도지만 roaster 표면의 실제 온도는 약 $2000^{\circ}C$에 이르는데 표면과 접촉한 부분에서 $B({\alpha})P$가 생성된다. $B({\alpha})P$는 주로 음식을 조리, 가공할 때 식품의 주성분인 탄수화물, 단백질, 지방 등이 열분해 되어 생성되는 것으로 생각된다. The following is the study result of herbal tea roasted at different temperatures between $80{\sim}140^{\circ}C$. Depending on treatment temperature the water content decreased, some carbonization occurred and crude ash content relatively increased. Also crude protein and crude fat decreased little. Solid elution rate of herbal tea showed 0.15~0.32%(w/w) and the rate of solid elution decreased with higher roasting temperature. There was no big change in $80{\sim}110^{\circ}C$ treatment section but the solid elution decreased rapidly in $110{\sim}140^{\circ}C$ section. The reason for decreasing solid elution rate at higher treatment temperature is because the compact inner tissue makes elution difficult. Benzopyrene content (0.29~0.51ppb) showed a tendency to increase with higher treating temperature. From this result, the $B({\alpha})P$ content differed depending on the treatment temperature and raw materials. In case of roasting, the actual inside temperature is around $200^{\circ}C$ but since the surface temperature of the roaster reaches around $2000^{\circ}C$ some portion of $B({\alpha})P$ content was presumed to be produced from the area that came in contact with this surface. When the processing which is a main component of food carbohydrate, protein, fat reason despite serve heat treatment as a whole is to be detected even though the $B({\alpha})P$ in this way is considered to be.

한국 녹차 화분의 화학적 조성과 기능성 연구

장재선,Jang, Jae-Seon 한국식품영양학회 2018 韓國食品營養學會誌 Vol.31 No.1

In this study, we investigated the nutritional composition including proximate, amino acid, vitamin, minerals, and the antioxidant activity of green tea (Camellia sinensis) pollen grains collected by Apis mellifera bees, for use as a health food. The crude protein and fat content was estimated at 26.14% and 3.49%, respectively. Eighteen amino acids were identified in green tea pollen, including 8 essential amino acids and 10 non-essential amino acids. The predominant amino acids were glutamic acid, proline and aspartic acid accounting for about 33.3% of total free amino acids. The concentration of vitamin C was the highest value of 35.7%, followed by $B_3$ and $B_2$ among the detected vitamins. The predominant minerals were potassium (790.32 mg/100g), followed by phosphorus (707.52 mg/100g) and sulfur (302.67 mg/100g), whereas copper, zinc and sodium were detected as minor elements. The antioxidant activity and phenolic content accounted for 33.8% at $500{\mu}g/mL$ extract and $2.55 {\mu}g/mg$, respectively.