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B형 간염 바이러스 Pre - S2 Peptide 의 단일클론 항체의 생산
이영익,임선영,김영훈,윤미선,함경수,한문희 ( Young Ik Lee,Suhn Young Im,Young Hoon Kim,Mi Seon Yoon,Kyong Soo Hahm,Moon Hi Han ) 생화학분자생물학회 1989 BMB Reports Vol.22 No.3
Monoclonal antibody against HBV pre-S2 peptide was generated. Six clones among 70 hybridomas which were survived in HAT media produced anti pre-S2 antibody. All six clones has been identified to produce IgM-type monoclonal antibody which has kappa light chain after ELISA subtyping analysis. After purification of IgM monoclonal antibody, heavy and light chain of immunoglobulin has been identified whose molecular weights are 79KD and 26.5KD, respectively. These pre-S2 antibodies were confirmed using immunoblotting techniques. IgM type pre-S2 monoclonal antibody were bound to 55 amino acid pre-S2 antigen whose M.W. is 55KD.
Vibrio vulnificus의 인혈청살균력에 대한 감수성과 Vibrio 감염이 마우스의 Hematocrit치에 미치는 영향
하대유,임선영,전상남,김철기,Ha, Tai-You,Im, Suhn-Young,Chun, Sang-Nam,Kim, Chul-Kee 대한미생물학회 1986 大韓微生物學會誌 Vol.21 No.3
Vibrio vulnificus, a halophilic Vibrio has gained worldwide attention as a cause of severe and frequently fatal wound infections and life-threatening septicemia. For this reason V. vulnificus is thought to produce extreme hemoconcentration and rapid death after infection, and because V. vulnificus is thought to be less susceptible to bactericidal activity of normal human serum, the present study was undertaken to assess the susceptibility of V. vulnificus to human serum bactericidal activity with that of V. parahemolyticus and V. cholerae and to assess the effect of Vibrio species, Salmonella typhimurium and E. coli on hematocrit values in experimentally infected mice. Serum bactericidal activity against both V. vulnificus and V. cholerae was higher than against V. parahemoltyicus. Survival of the test strains in heat-inactivated human serum was greater than that in heat-uninactiveted serum. Both V. parahemolyticus and V. cholerae produced slight hemoconcentration within 2 to 6 hr after intraperitoneal injection of $10^7$ viable bacteria into mice. In contrast, V. vulnificus, S. typhimurium and E. coli produced hemodilution rather than hemoconcentration after 4 or 6 hr after infection. With these results the author can conclude that V. vulnificus is more susceptible to serum bactericidal activity than other Vibrio species, and V. vulnificus did not produce hemoconcentration.
Generation of Monoclonal Antibody Against Hepatitis B virus (HBV) Pre-S2 Peptide
이영익,임선영,김영훈,윤미선,함경수,한문희,Lee, Young-Ik,Im, Suhn-Young,Kim, Young-Hoon,Yoon, Mi-Seon,Hahm, Kyong-Soo,Han, Moon-Hi 생화학분자생물학회 1989 한국생화학회지 Vol.22 No.3
대장균에서 재조합 발현된 Hepatitis B virus 표면항원 Pre-$S_2$ peptide로 면역시킨 Balb/c 마우스 비장세포와 SP2/O-Ag 14 미에로마 세포를 융합시켜 Pre-$S_2$에 대한 단일클론 항체를 생산하였다. HAT 선택배지에서 자란 70개의 융합세포군 중 Pre-$S_2$에 대한 항체를 생산하는 6개의 융합세포군을 제한 희석법으로 클로닝하였으며 ELISA 방법을 이용하여 이들 6클론을 isotype 한 결과 모두 kappa light chain을 갖는 IgM이었다. 이들 항체의 항원특이성은 western bolt를 이용하여 55 amino acid Pre-$S_2$ peptide와 결합함을 확인하였다. Monoclonal antibody against HBV pre-S2 peptide was generated. Six clones among 70 hybridomas which were survived in HAT media produced anti pre-S2 antibody. All six clones has been identified to produce IgM-type monoclonal antibody which has kappa light chain after ELISA subtyping analysis. After purification of IgM monoclonal antibody, heavy and light chain of immunoglobulin has been identified whose molecular weights are 79KD and 26.5KD, respectively. These pre-S2 antibodies were confirmed using immunoblotting techniques. IgM type pre-S2 monoclonal antibody were bound to 55 amino acid pre-S2 antigen whose M.W. is 55KD.
인체 인터루킨 - 2 - ( IL - 2 ) Peptide 의 단일클론 항체 생산
이영익,김영훈,임선영,함경수,염현정,한문희 ( Young Ik Lee,Young Hoon Kim,Suhn Yong Im,Hyeon Jeong Yeom,Kyong So Hahm,Moon Hi Han ) 생화학분자생물학회 1991 BMB Reports Vol.24 No.2
Monoclonal antibody against Interleukin-2 peptide was generated. One hundred four hybridomas which were survived in HAT media produced anti-IL2 antibody. Among 104 hybridomas, eleven clones has been identified to produce IgG1 type monoclonal antibody which has kappa light chain after ELISA subtyping analysis. After purification of IgG1 monoclonal antibody, heavy and light chain of immunoglobulin has been identified whose molecular weights are 55 kD and 25 kD, respectively. These IL-2 antibodies were confirmed using immunoblotting techniques. IgGl type IL-2 monoclonal antibody were bound to 133 amino acids IL-2 antigen whose M.W. is 15 kD. These monoclonal antibodies were used for purifying IL-2 peptides. IL-2 ELISA systems were generated using immuno-affinity purified IL-2 as antigens.