대장암 동물 모델에 대한 이해

임도영,Lim, Do Young 제주대학교 의과학연구소 2018 The Journal of Medicine and Life Science Vol.15 No.2

Colorectal cancer (CRC) is a third leading cause of cancer-related death in cancer patients. Sporadic and inflammation-related colon carcinogenesis are major mechanism of colorectal cancer. In vivo CRC models have been developed and implicated to understand their mechanisms upon a different type of CRC. Moreover, recently animal models have played important roles in chemopreventive and preclinical trials over the years. In this mini-review, the aim is to introduce various animal models of CRC and help the understanding to establish in vivo experimental plans according to the cancer type of CRC.

국내 가축분뇨 자원화 특성을 고려한 OECD 질소수지 산정법의 지역단위 적용 연구

임도영,류홍덕,정유진,김용석,이재관,Lim, Do Young,Ryu, Hong-Duck,Chung, Eu Gene,Kim, Yongseok,Lee, Jae Kwan 한국물환경학회 2017 한국물환경학회지 Vol.33 No.5

The Nutrient budget is one of the agricultural-environment indicators of OECD. A nutrient budget measures the surplus as the differential between the inputs and the outputs of within a certain boundary and within a specified period of time (i.e. one year). According to OECD, the annual nitrogen budget for Korea was $245kg\;N\;ha^{-1}$ in 2014, which corresponds to the first position among OECD countries. In Korea in 2014, about 90 % of livestock excreta was composted as solid and liquid manure, which are usually and customarily spread on agricultural land. The objectives of this study are intended to suggest methodology of the regional nitrogen budget as a nitrogen management tool, which considers conversion from raw excreta to composted manures based on the methodology of OECD/Eurostat, and application of the new method in an agricultural region of Korea. As a result, the calculated excess rate of hydrospheric nitrogen surplus was $251kg\;N\;ha^{-1}$ (in the region in 2014), which indicates the presence of potential risks emanating from excessive nitrogen, with regard to both export water and soil environments. The findings also assert that this was shown to be one of the most important elements in the nitrogen budget, which translates to the actual amounts of nitrogen lost during the solid composting process. To better understand the process and the reliability of the method, it is necessary to analyze the sensitivity of the relevant co-efficients used in the method in the near future.

OECD 인 수지 산정법의 지역단위 적용 연구: 유출입 자료 출처 비교

임도영 ( Do Young Lim ),류홍덕 ( Hong-duck Ryu ),정유진 ( Eu Gene Chung ),김용석 ( Yongseok Kim ) 한국환경과학회 2017 한국환경과학회지 Vol.26 No.11

Phosphorus (P) is an essential and major nutrient for both plants and animals. However, anthropogenic P in the environment may cause severe problems such as the deterioration of water quality. Therefore, it is essential for the Korean government to manage P in the agricultural sector. The annual P budget for Korea was 46 kg P ha-1 in 2013, placing Korea in second among Organisation for Economic Co-operation and Development (OECD) countries. P surplus and deficiency in agricultural lands can be estimated according to the P budget, which is one of the OECD agri-environment indicators. In the P budget, it is important to ensure consistency in the input-output data sources, in order to apply national and regional policies for the environmentally sound management of agricultural P. This study examines the impacts on the input-output data sources in the regional P budget in Korea. P budgets were between 99-145 kg-P/ha, depending on different data sources. We suggest two recommended data combinations (DC 1 and DC 2) for reliability of the data. P budgets calculated using DC 1 and DC 2 were 128 kg-P/ha and 97 kg-P/ha, respectively. According to the results, one of the core factors affecting P budgets was crop production. In this study, DC 2 was recommended rather than DC 1 in order to consider the cultivated areas for various crops. It is also necessary to analyze the sensitivity of the coefficients used in P budget in the future.

초등학교시설의 노인여가복지시설 복합화 적합성 평가

임도영(Lim, Do-Young),송병준(Song, Byung-Joon),주범(Chu, Beom) 한국실내디자인학회 2015 한국실내디자인학회논문집 Vol.24 No.6

This study attempts to deduce the necessary and validity of complexation, and suggests complexation through complexation suitability evaluations as follows. First, the concept and current state of leisure facilities for the elderly and elementary facilities and architectural complexation were investigated through theoretical considerations. Second, the target schools for complexation of `elementary school-leisure facility for the elderly` were selected. The aspects of facility introduction of an elementary school, teacher support facility, convenience facility for the disabled, safety and security facilities as well as school facility accessibility were investigated on the basis of the data attained from theoretical considerations. Third, the feasibility of complexation of leisure facilities for the elderly was reviewed, based on the investigated data on complexation-targeted elementary school. Regarding school building and support facilities, with a focus on leisure programs for the elderly, the availability and number of rooms, extra numbers of regular classrooms and the room condition were assessed on the basis of the analysis of operational perspective of each facility. The convenience facilities for the disabled were examined by classifying them into interventional facility in consideration of facilities for the elderly, pursuant to the provisions in the convenience promotion assurance act for the disabled, elderly and pregnant women. Safety and security facilities were evaluated in an effort to assess the safety issue of the elderly and children. The result of the evaluation of the 6 elementary schools in Seong-buk county based on the above-mentioned four measurement standards shows that they scored 107~124 points of the total 155 points, with the average of 118.5 points. This is more than the medium score and shows the possible complexation of the elementary school facilities as the leisure facilities for the elderly, and at the same time it may lead each school to find out the elements conductive to enhancing the complexation as a result of the evaluation. Through the evaluation data, I expect that we contribute to promoting the process of the evaluation of the appropriateness of complexation and the efficient complexation.

가축분뇨실태조사를 위한 질소수지 모델 민감도 분석

임도영 ( Do Young Lim ),류홍덕 ( Hong-duck Ryu ),정유진 ( Eu Gene Chung ),김용석 ( Yongseok Kim ),이재관 ( Jae Kwan Lee ) 한국물환경학회(구 한국수질보전학회) 2018 한국물환경학회·대한상하수도학회 공동 춘계학술발표회 Vol.2018 No.-

경력단절 여성의 진로 연구에 관한 통합적 문헌고찰: 구성주의 진로이론의 진로적응모형을 중심으로

임도영(Do-Young Lim),김성길(Seong-Gil Kim) 한국진로교육학회 2020 진로교육연구 Vol.33 No.2

본 연구의 목적은 국내 경력단절 여성의 진로 연구 문헌에서 구성주의 진로이론 기반의 진로적응모형의 틀을 적용하여 연구 변인을 분류하고, 진로적응모형 변인 분류에 따른 연구 현황과 진로적응성 연구의 특징을 확인하는데 있다. 이를 위해 2010∼2019년까지 발표된 연구들의 논문을 Torraco(2005)가 제시한 통합적 문헌고찰의 방법을 활용하여 총 69편을 분석하였다. 분석 결과, 첫째, 진로적응모형의 적응준비, 적응자원, 적응반응, 적응결과의 범주로 확인된 변인은 총 51개였으며, 적응준비 변인이 30개로 가장 많았다. 연구된 변인의 빈도를 확인한 결과 진로결정자기효능감, 진로준비행동, 진로장벽, 사회적지지, 진로적응성, 역할지향성 순으로 나타났다. 둘째, 연구주제별로는 프로그램개발·교육 경험 관련 효과성을 살펴본 연구가 20편(29%), 진로적응성 연구 12편(17%)이었으며, 적응자원에서의 환경변인은 진로장벽과 사회적지지가, 적응반응 변인에서 진로결정자기효능감, 진로준비행동이 가장 많이 연구되었다. 본 연구결과를 중심으로 우리나라 경력단절 여성의 진로 연구의 의의 및 제한점과 추후 경력단절 여성의 진로 연구를 위한 제언을 제시하였다. The purpose of this study is to classify research variables by applying the theoretical framework of the career adaptation model based on constructivism career theory in domestic career interrupted women’s career research literature, and to identify the current status of research and the characteristics of career adaptation research. Therefore, this study analyzed 69 research papers published in Korea from 2010 to 2019, using the integrated literature method presented by Torraco (2005). According to the analysis, first, a total 51 of variables were identified as the four categories of career adaptation model-adaptivity readiness, adaptability resources adapting-response, and adaption results. Among 51 of variables, 30 variables of them were categorized by adaption variables which is the largest number of variables in four career adaption model’s categories. In terms of frequency of variables, career-setting self-efficacy made up the greatest portion of variables, followed by career preparation behavior, career barriers, social support, career adaptability and role orientation. Secondly, with regard to research topic, there were twenties studies on the effectiveness of program development and education experiences(29%) and twelve studies on career adaptation(17%). While in the environmental variables of adaptation resources were focused on career barriers and social support, and in adaptive response variables were focused on career decision-making self-efficacy and career preparation behavior. Based on the results of this study, this study points out the significance and limitations of research related to career adaptation of career-interrupted women in Korea, and present suggestions for conducting further research on career-Interrupted married women.

목향 헥산추출물의 LNCaP 전립선암세포 증식 억제 효과

박소영(So Young Park),김은지(Eun Ji Kim),임도영(Do Young Lim),김정상(Jong-Sang Kim),임순성(Soon Sung Lim),신현경(Hyun-Kyung Shin),윤정한(Jung Han Yoon) 한국식품영양과학회 2008 한국식품영양과학회지 Vol.37 No.1

목향은 국화과에 속한 다년생 식물인 Saussurea lappa의 뿌리로서 한의학에서는 구토, 설사 및 염증치료 등에 사용되고 있다. 목향 추출물 및 그 성분들은 항균 작용, 항염증 작용, 혈관생성 억제 효능 등을 지니고 있으며 위암과 대장암의 세포증식을 억제하는 것으로 알려져 있다. 그러나 현재까지 전립선암에 대한 목향의 효과는 연구된 바 없다. 본 연구에서는 목향이 전립선 암세포에 미치는 영향을 조사하기 위해 인간의 전립선에서 유래한 암세포인 LNCaP 세포의 증식과 apoptosis에 미치는 영향을 조사하였다. 목향 헥산추출물을 LNCaP 세포 배양액에 여러 농도(0~4 ㎎/ℓ)로 첨가하여 세포의 증식에 미치는 영향을 조사하였다. 목향 헥산추출물의 농도가 증가할수록 LNCaP 세포의 증식은 현저하게 감소하였고 apoptosis는 증가함을 관찰하였다. 목향 헥산추출물이 LNCaP 세포의 apoptosis 일으키는 기전을 연구하기 위하여 목향 헥산추출물을 첨가하고 세포를 48시간 배양한 후 cell lysate를 얻어 Western blot을 실시하였다. Apoptosis 과정에 작용하는 중요한 단백질 중 하나인 Bcl-2 family 단백질 중 pro-apoptotic Bcl-2 단백질인 Bak와 BH3 only Bcl-2 단백질인 truncated-Bid의 단백질 수준은 목향 헥산추출물에 의해 유의적으로 증가한 반면 anti-apoptotic Bcl-2 단백질인 Bcl-2, Bcl-xL 및 Mcl-1 단백질 수준은 변하지 않았다. 또한 apoptosis를 집행하는 caspase의 활성 형태인 cleaved caspase-8, -9, -7, -3의 단백질 수준이 목향헥산추출물의 처리에 의해 증가하였고 caspase-3의 표적 단백질 중 하나인 PARP의 불활성 형태인 cleaved PARP의 단백질 수준도 현저하게 증가하였다. 이 결과들은 목향 헥산추출물이 LNCaP 세포의 apoptosis를 유도함으로써 전립선 암세포의 증식을 억제함을 보여주는 것이며 목향 헥산추출물에 의한 apoptosis 유도는 caspase 활성 증가와 Bak 및 t-Bid 단백질의 증가에 의한 것임을 제시한다. 따라서 앞으로 항암효과를 나타내는 성분의 동정 및 동물실험을 통하여 좀 더 면밀한 기전 연구가 수행된다면 목향 헥산추출물은 화학적 암예방 물질이나 치료제로 개발될 수 있을 것으로 사료된다. Saussurea lappa (SL) has been used to reduce abdominal pain and tenesmus in traditional oriental medicine. SL and major compounds of SL, sesquiterpene lactones, have been suggested to possess various biological effects, including anti-tumor, anti-ulcer, anti-inflammatory, anti-viral and cardiotonic activities. Recently, it has been reported that ethanol extracts from roots of SL have antiproliferative effects on gastric cancer cells. To explore the possibility that SL has chemopreventive effects on prostate cancer, we examined whether the hexane extract of SL (HESL) inhibits the growth of LNCaP human prostate cancer cells. Cells were incubated with various concentrations (0~4 ㎎/ℓ) of HESL in DMEM/F12 containing 5% charcoal stripped fetal bovine serum. HESL substantially decreased viable cell numbers and induced apoptosis of LNCaP cells in dose-dependent manners. HESL increased the levels of cleaved caspase-8, -9, -7 and -3, and poly (ADP-ribose) polymerase. HESL increased the levels of the pro-apoptotic Bak and truncated-Bid proteins whereas it had no effect on the anti-apoptotic Bcl-2, Bcl-xL, or Mcl-1. The present results indicate that HESL inhibits the growth of human prostate cancer cells by inducing apoptosis, which involves the activation of the caspase cascades.

질산염 안정동위원소 비를 활용한 가축사육 밀집 농촌지역 지하수의 질산염 오염원 추적

김록영(Rog-Young Kim),백운일(Un Il Baek),김선정(Sun Jung Kim),임도영(Do Young Lim),김덕우(Deok-Woo Kim),김민섭(Minseop Kim),김경현(Kyunghyun Kim),나은혜(Eun Hye Na),김용석(Yong-Seok Kim),정유진(Eu Gene Chung) 대한환경공학회 2022 대한환경공학회 학술발표논문집 Vol.2022 No.11

공간적 평가기법을 활용한 유역 단위 양분관리 대상지역 우선순위 도출

김덕우(Deok-Woo Kim),임도영(Do Young Lim),김록영(Rog-Young Kim),나은혜(Eun Hye Na),김용석(Yongseok Kim),정유진(Eu Gene Chung) 대한환경공학회 2022 대한환경공학회 학술발표논문집 Vol.2022 No.11

간헐포기 활성슬러지 공정에서 유입 유기탄소원에 따른 생물학적 영양염류 제거에 관한 연구

김종민(Jong Min Kim),임도영(Do Young Lim),고광백(Kwang Baik Ko),정연규(Youn Kyoo Choung) 한국물환경학회 1999 한국물환경학회지 Vol.15 No.2

Effects of various organic carbon sources on biological nutrient removal were investigated. Acclimated activated sludges were supplied in synthetic wastewater in a series of lab-scale sequencing batch reactors, which were operated in the 2 hour anoxic and 4 hour aerobic mode during a 12 hour period. The results show that phosphorus release and uptake occurred both in a culture fed with glucose and in one fed with sodium acetate. However, the rate of phosphorus release and uptake was higher in the culture fed with sodium acetate than in the culture fed with glucose. Variation of organic carbon sources in influent did not inhibit nitrification in any rector. Relatively significant denitrification was observed in all reactors in the beginning of aerobic phase since DO concentrations were rather low and organic content was relatively high during this period. As G bacteria might outnumber Poly-P bacteria in a culture fed with a mixture of glucose and sodium acetate. the rate of phosphorus release and uptake was lower in the culture than in a culture fed with sodium acetate only.