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녹차의 에탄올 추출물이 암세포주의 형태학적변화에 마치는 영향
장원태 ( Won Tae Jang ),이효권 ( Hyo Kwon Lee ),김미형 ( Mi Hyung Kim ),류인 ( Yin Liu ),최원경 ( Won Kyung Choi ),성훈기 ( Hoon Ki Sung ),박정현 ( Jeong Hyun Park ) 대한임상검사과학회 2000 대한임상검사과학회지(KJCLS) Vol.32 No.3
Green tea is one of most popular traditional teas in korea. ``The purpose of this study was comparing the effects of ethanol extracts on three kinds of cancer cell lines(HepG2, A549, EATC) according to its concentration and exposure time. For this purpose, we obseπed morphological changes under inverted and electron microscope and anallysed cytotoxicity . EATC(Ehrlich-Lettre Ascites Tumor Cell) cells were damaged by 100μg/m1 ethanol extracts treatment until 72 hours gradually. In A549 cells, ethanol extracts caused severe cellular injury abrupt1y and lasted for 72 hours. However, cellular necrosis and growth inhibition in both of them could not be observed in 1, 10μg/ml ethanol extracts administration. Whereas, the effects of that were weak in all of concentrations and exposure times in HepG2 cells. In conclusion, green tea ethan이 extracts induced strong inhibition of growth and severe cellular damages in A549(Lung Cancer) and EATC except HepG2(Hepatoblastoma). In addition, its effects were dependent on concentration and duration of ethanol extracts. Therefore, though there were some different effect according to cancer type, it suggested that green tea would be helpful to prevent carcinogenesis and treat cancer.