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β-Carotene Hydroxylase 관련 Chyb 유전자를 이용한 형질전환 Arabidopsis에서 Astaxanthin의 생합성
이호재,강권규,Lee, Ho-Jae,Kang, Kwon-Kyoo 한국식물생명공학회 2004 식물생명공학회지 Vol.31 No.3
Oxycarotenoids는 녹색식물, 곰팡이, 효모, 버섯 및 세균 등이 만들어 내는 황색, 적색 또는 자색의 polyene계 색소로 분자내에 산소를 함유하며 생체내에서 중요한 역할을 담당하고 있다. 본 실험에서는 Oxycarotenoids의 생합성 경로상에 존재하는 $\beta$-carotene hydroxylase 유전자 (Chyb)가 재조합된 Ti-plasmid (pGCHYB)를 A. tumerfacience GV3101에 의해 Arabidopsis thaliana (cv. Columbia)에 형질전환하였다. 50 mg/L hygromycin 함유한 MS 배지에서 선발된 개체를 이용하여 Chyb 유전자의 도입여부를 PCR로 분석한 결과, 대조구에서는 Chyb 유전자의 증폭 되지 않았으나 형질전환체에서는 증폭 산물을 확인 할 수 있었다. 또한 형질전환체의 발현여부를 RT-PCR분석한 결과 도입된 Chyb 유전자가 안정적으로 발현되었다. 형질전환체의 carotenoids를 HPLC 분석한 결과 xanthophyll cycle carotenoids (violaxanthin과 zeaxanthin)의 함량 및 $\beta$-carotene 함량은 감소되었으며, 대조구 Arabidopsis에는 생합성되지 않는 astaxanthin이 생합성되었다. 따라서 본 실험에서 육성된 형질전환체를 이용하여 oxycarotenoids 생합성 과정상의 중간대사물질의 표지, 관여된 transcript 및 metabolite 분석 등을 통해 carotenoids 대사계의 연구소재로 활용 할 수 있을 것으로 기대한다. Oxycarotenoids are oxygenated carotenoids that perform critical roles in plants. $\beta$-Carotene hydroxylase adds hydroxyl groups to the $\beta$-rings of carotenes and has been cloned from several bacteria and plants including Arabidopsis. This study was carried out to investigate the effect of $\beta$-carotene hydroxylase gene (Chyb) on the oxycarotenoids biosynthesis in the transgenic Arabidopsis. Construct of pGCHYB containing Chyb was established onto Gateway vector system (pENTR3C gateway vector and pH2GW7 destination vector). Arabidopsis thaliana (cv. Columbia) was transformed with Agrobacterium tumerfacience GV3101 harboring pGCHYB construct driven by 35S promoter and hygromycin resistant gene. Seven hundred bases paired PCR products, indicating the presence of Chyb gene, were found in the transformants by PCR analysis using Chyb primers. Hygromycin resistance assay showed that transgenes were stably inherited to next generation. The overexpression of the Chyb gene resulted in the decrease carotenoid content. Especially, astaxanthin unusual oxycarotenoid in wild type Arabidopsis was detected in the transgenic plants. This means that decreased carotenoids might be converted into astaxanthin metabolism with the aid of silent gene in the host.
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테트라싸이클린 처리된 흡수성 및 비흡수성 조직유도재생술용 막에의 세균부착과 침투양상
이호재,정현주,Lee, Ho-Jae,Chung, Hyun-Ju 대한치주과학회 1997 Journal of Periodontal & Implant Science Vol.27 No.1
The barrier membranes for GTR procedure could be affected bY bacterial contamination after exposure to oral environment. This study was done to evaluate whether the tetracycline impregnated barrier membranes could inhibit bacterial attachment and penetration into membranes. The resorbable membrane(polylactic and polyglycolide copolymer, $Resolute^{(R)}$, W.L Gore and Associates, Inc..USA) and the non-resorbable membrane(e-PTFE; Gore-TexTM, W.L. Gore & Associates, Inc.,USA) were cut into 4mm discs and trated with 5% tridodecylmethylammonium chloride solution in ethanol and dried in air. The membranes were immersed in tetracycline(TC) solution (100mg/ml, pH 8.0) and dried. To the maxillary canine-premolar region in six periodontally healthy volunteers, removable acrylic devices were inserted, on which 8 cylindrical chambers were glued with TC impregnated and non-impregnated discs, the membrane discs were examined for bacterial attachment and penetration, and structural changes under SEM and LM. From the 1st day to the 7th day, membranes showed bacterial plaque formation composed of cocci and rods. Thereafter, filamentous bacteria appeared and the plaque thickness increased. The TC impregnated e-PTFE membranes showed less bacterial attachment and delayed in bacterial plaque maturation than non-treated membranes. As for bacterial penetration, the TC impregnated e-PTFE membranes showed superficial invasion and infrequent presence of bacteria in unexposed inner surface at the 4th week. while the non-treated e-PTFE membranes showed deep bacterial invasion at the 2nd week and frequent presence of internal bacteria at the 4th week. The resorbable membranes started to be resorbed at the 2nd week and were perforated at the 4th week, regardless of TC treatment. In conclusion, bacterial plaque formation and penetration was efficiently delayed in TC impregnated e-PTFE membranes, whereas resorbable membranes were similar in bacterial invasion due to membrane degradation and perforation, regardless of TC treatment.
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이호재,김홍주,김재근,Lee, Ho-Jae,Kim, Hong-Ju,Kim, Jae-Geun 한국전자통신연구원 1992 전자통신 Vol.14 No.2
본고에서는 CCITT 표준에 기본을 둔 다양한 전송서비스 능력을 갖는 SDH 망요소들의 모듈화 구현기법을 제시하고, 나아가 이들 망요소들을 응용한 동기식 전송망의 구조를 전송서비스의 보호특성을 강조하여 제시한다.
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이호재,허웅,Lee, Ho-Jae,Huh, Woong 대한전자공학회 1996 전자공학회논문지-B Vol.b33 No.4
In this paper, we implemented a computerized pulse diagnosis system for the developement of pulse diagnosis algorithms and the generalization of the pulse diagnosis. The system consists of the hardware and software. The hardware detects pulse waves and inputs the waves into the computer system, while the software not only manages and analyzes the input pulse wave data but also privides the database. In order to clinically test the developed pulse diagnosis system, we applied the carotid-radial pulse diagnosis algorithm to the system. As the results, it is found that the genralizatio of the pulse diagnosis is possible.
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이호재,박영배,허웅,Lee, Ho-Jae,Park, Young-Bae,Huh, Woong 대한의용생체공학회 1993 의공학회지 Vol.14 No.1
This paper describes the implementation of a computerized radial pulse diagnosis by Elds of a clinical expert. On this base, we composed of the radial pulse diagnosis system in korean traditional medicine. The system composed of a radial pulse wave detection system and a radial pulse diag nosis system. With a detection system, we detected Inyoung and Cheongu radial pulse wave and processed It. Then, we have got the characteristic parameters of radial pulse wave and also quantified thats according to the method of Inyoung-Cheongu Comparison Radial Pulse Diagnosis. We defined the jugement standard of radial Pulse diagnosis system and then we confirmed the PossibiliLy for realization of automatic radial Pulse diagnosis in korean traditional medicine.
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백서 치주인대세포의 분화에 대한 Bone morphogenetic protein-7의 영향
이호재,김영준,정현주,Lee, Ho-Jae,Kim, Young-Jun,Chung, Hyun-Ju 대한치주과학회 2005 Journal of Periodontal & Implant Science Vol.35 No.3
Periodontal therapy has dealt primarily with attempts at arresting progression of disease. however, more recent techniques have focused on regenerating the periodontal ligament having the capacity to regenerate the periodontium. Recombinant human bone morphogenetic protein-7(rhBMP-7) can differentiate the osteoprogenitor cells and induce bone formation. The purpose of this study was to evaluate the effect of BMP-7 on rat periodontal ligament cells differentiation, in vitro. In the control group, cells was cultured with DMEM media. In the experimental groups, cells were cultured with rhBMP-7 in concentration of 10, 25, 50 and 100 ng/ml. Each group was characterized by examining alkaline phosphatase activity at 3 and 5 days of culture and the ability to produce mineralized nodules of rat calvarial cells at 14 days of culture. Synthesis of type I collagen(COL-I), osteocalcin(OCN), and bone sialoprotein(BSP) was evaluated by RT-PCR at 7 days of culture. Activation of Smad proteins and p38 MAP kinase was determined by western blot analysis of the cell lysates. Alkaline phosphatase activity was significantly increased in the concentration of BMP-7 50 ng/ml and 100 ng/ml compared to the control(p<0.05). The mineralized bone nodule formation was greater with addition of 50 ng/ml and 100 ng/ml BMP-7 than the control(p<0.01). In 7 days' culture, the expressions of COL-I, BSP, and OCN was increased by BMP-7 in concentration of 10 $ng/ml{\sim}100$ ng/ml. In western blot analysis, BMP-7 treated culture cells expressed Smad 1,5,8 in dose-dependent manner, whereas BMP-7 did not activate phosphorylated form of p38 MAP kinase. These result suggested that BMP-7 stimulate rat periodontal ligament cells to differentiate toward osteoblast phenotype and increase bone matrix production by activation of BMP-Smad pathway.
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이호재(Lee, Ho-Jae) 혜암신학연구소 2018 신학과교회 Vol.10 No.-
This paper examines the meaning of salvation in Confucianism, Buddhism, and Taoism, which are the traditional religions of Korea. The scope of the study covers religions that were present in Korea before Christianity was introduced into the country. Under the traditional religious category, a semantic system similar to ‘salvation’ in Christianity, which is present in the respective religions of Confucianism, Buddhism, and Taoism, is discussed from a religious viewpoint. The religious landscape in Korea does not indiscriminately accept foreign religions. Emphasis is placed on selection based on religious traits such as Fung-Ryu( 風流性 ) or Muism( 巫敎性 ) in Korea. Fung-Ryu( 風流性 ) acts as a positive mechanism to create a creative religious culture, whereas Muism( 巫敎性 ) acts as a passive and negative mechanism. Fung-Ryu( 風流 性 ) or Muism( 巫敎性 ) form the essence and core of religion in Korea and function as a frame of reference for the acceptance and interpretation of foreign religions.This clearly concludes that traditional religions like Confucianism, Buddhism, and Taoism have religious systems similar to Christianity. Though not equivalent to the salvation in Christianity, traditional religions have been categorized as having a corresponding salvation system in the study. Firstly, Confucianism consists of world views that are realityoriented. Through Confucian rituals such as the “Four Ceremonies” of Capping, Wedding, Funeral and Ancestral Rites, confucianism followers inherit and propagate a community of filial piety in the form of clans, seeking salvation within an everlasting community of remembrance. Secondly, Mass Buddhism pursues the salvation views of ‘Here and Now’ that create a world of Nirvana in coexistence with all living creatures by Bodhisattva Conduct and breaking away from Pratītyasamutpāda and retributive justice. Thirdly, although the development of Taoism into a religious body is limited by the nature of the religious climate in Korea, focus has been placed on Taoism as a religious system that seeks immortal salvation through the creation of a universal body in combination with the way of eternal life. Thus, it can be deduced that the ultimate human character pursued in traditional religions, though non-interfering with the views of certain religions, is made up of religious views that realize permanent existence through the religions own belief and ritual systems. This shows that Confucianism, Buddhism, and Taoism are formed as one whole religious system under each respective religious and cultural contextual system, and that permanent salvation is a phenomenon that works within the meaning and ritual system framework of each religion.
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