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생체내 이식된 배아줄기세포 유래 세포의 추적을 위한 형광표지 배아줄기세포주의 수립
김은경 ( Eun Kyoung Kim ),이형희 ( Hyung Hee Lee ),오구택 ( Goo Taeg Oh ),윤미정 ( Mi Chung Yoon ),이영희 ( Young Hee Lee ),김형진 ( Hyoung Chin Kim ),최양규 ( Yang Kyu Choi ),이철호 ( Chul Ho Lee ),남기환 ( Ki Hoan Nam ) 한국조직공학과 재생의학회 2004 조직공학과 재생의학 Vol.1 No.2
It is important to chase the cells injected into animal body for evaluation of cell therapy. We established EGFP labeled mouse embryonic stem cells that could be easily detected by their fluorescence expression in frozen tissue sections. The established embryonic stem cells expressed EGFP even after differentiated into various types of cells. They also could form EGFP expressing teratomas when they were injected into NOD-scid mice or nude mice. When these cells were injected into peripheral blood of ICR mice, the cells disappeared within 30 sec from the peripheral blood. The injected cells were found mainly in the lung when injected through tail vain. However, they were evenly distributed onto organs when injected into the left ventricle. These cells could be detected by fluorescence microscopy, flow cytometric analysis or confocal microscopy without any treatment on the cells. Accordingly, the embryonic stem cells labeled with EGFP might be very useful in analyzing cells derived from embryonic stem cells in mixed cell culture in vitro, and also very helpful in analyzing transplanted cells derived from differentiated embryonic stem cells.
GGEx16, GGEx18과 감비통성교낭(減肥通聖膠囊)의 항비만유전자 활성 비교
오재호 ( Jae Ho Oh ),안예지 ( Ye Ji Ahn ),이혜림 ( Hye Rim Lee ),임혜숙 ( Hye Sook Lim ),이형희 ( Hyung Hee Lee ),윤미정 ( Mi Chung Yoon ),신순식 ( Soon Shik Shin ) 대한본초학회 2013 大韓本草學會誌 Vol.28 No.2
Objectives: Gambigyeongsinhwan 16 (GGEx16), gambigyeongsinhwan 18 (GGEx18) and gambitongseong capsule are shown to be involved in the regulation of obesity. Therefore, the aim of this study was to compare the reporter activity of anti-obesity genes such as peroxisome proliferator-activated receptor a (PPARa) and PPARδ by GGEx16, GGEx18 and gambitongseong capsule. Methods: After NMu2Li liver cells, C2C12 skeletal muscle cells and 3T3-L1 preadipocytes were treated with GGEx16 (1 μg/ml), GGEx18 (1 μg/ml) and different concentrations of gambitongseong capsule, the transactivation of PPARa and PPARδ was measured by a luciferase reporter gene assay. Results: PPARa reporter gene activity in NMu2Li liver cells and 3T3-L1 preadipocytes was significantly increased by GGEx16, GGEx18 and gambitongseong capsule compared with control, whereas PPARa reporter gene activity in C2C12 skeletal muscle cells was significantly increased by GGEx18 only compared with control. Similarly, PPARδ reporter gene activity in 3T3-L1 preadipocytes was also significantly increased by GGEx18 compared with control. PPARδ reporter gene activity in C2C12 skeletal muscle cells was significantly increased by GGEx16 and GGEx18 compared with control although PPARδ reporter gene activity in NMu2Li liver cells was not changed by these three formulas. Conclusions: These results suggest that all three formulas have the ability to stimulate PPARa and PPARδ transactivation in animal cell lines with high metabolic rates. In particular, this effects were most prominent in GGEx18-treated cells. In addition, it is likely that GGEx18 may be used as an effective anti-obesity composition.
인삼추출물과 사포닌에 의한 peroxisome proliferator-activated receptor α transactivation의 억제
정선효,이형희,윤미정 목원대학교 자연과학연구소 2001 自然科學 硏究論文集 Vol.10 No.2
Peroxisome proliferator-activated receptor α(PPARα)와 인삼은 지질대사를 조절하며 동맥경화증에 효과적인 것으로 알려져 있다. 본 연구는 인삼이 PPARα의 기능에 미치는 영향과 인삼의 효과가 PPARα를 통해 조절될 수 있는지를 알아보기 위해서, in vitro에서, transient transfection assay를 이용하여 PPARα와 인삼의 상호작용을 조사하였다. 그 결과 인삼은 PPARα reporter gene의 발현을 억제하였으며, 인삼추출물과 사포닌 모두 PPARα ligand인 Wy14,643에 의한 PPARα reportor gene의 활성을 억제하였다. 따라서 이러한 결과는 인삼이 PPARα의 transactivation을 억제한다는 것을 시사한다. Peroxisome proliferatoractivated receptor α(PPARα) and ginseng are known to modulate lipid metabolism and to have beneficial effects on atherosclerosis. To determine if there is an interplay between PPARα and ginseng in the control of lipid metabolism, the regulation of PPARα function by ginseng was examined using the transient transfection assay. Ginseng extract and ginseng saponin inhibited, in vitro, PPARα reporter gene expression. Both ginseng extract and ginseng saponin also inhibited, PPARα reporter, luciferase activity by PPARα ligand Wy14,643. These results support that ginseng inhibits PPARα transactivation.