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보툴리눔 신경독소의 세포기반 역가시험법의 최근 연구 동향
이학섭 ( Hak Sup Lee ),김윤영 ( Yoon Young Kim ),장성수 ( Sung Su Jang ) 서울대학교 인구의학연구소 2013 人口醫學硏究論集 Vol.26 No.-
Botulinum neurotixins (BoNTs), causative agent of botulism, are the most potent natural toxins mainly produced by bacterium Clostridium botulinum, which is a Gram-positive, rod-shaped and obligate anaerobe. BoNT serotypes A and B are wildely used as pharmaceuticals to treat diverse neurological diseases and for cosmetic purpose. To date, gold standard essay for a measurement of BoNTs` Potency is the mouse LD50 (50% lethal dose) bioassay. This is the only potency assay approved by the FDA. Mouse LD50 bioassay, however, causes not only large error but high cost, and it also requires large number of mice and trained person who handles the procedure. In this reason, there is a need to develop a reliable assay in order to replace the mouse LD50 assay. An alternative assay capable of including full biological activity of BoNTs is cell based assay. In this review, we will summarize advantages and limitations of various BoNTs potency assays, focusing on cell based assays.
이학섭 ( Hak Sup Lee ),김윤영 ( Yoon Young Kim ),장성수 ( Sung Su Jang ) 서울대학교 인구의학연구소 2011 人口醫學硏究論集 Vol.24 No.-
Pain is the general symptom encountered in a diversity of diseases. Although pain mechanisms are nor fully understood, hyperexcitability of primary sensory neurons can lead to abnormal burst associated with pain. After injury to their axons, sensory neurons can show excitability by increased voltage-gated sodium ion channel expression and abnormal accumulation. Therefore, sodium channel blockers may be effective analgesics in the management of pain such as neuropathic pain, Also, tetrodotoxin (TTX), non-peptide neurotoxin produced by bacteria living within organs of marine organism such as puffer fish, is a potent sodium ion channel blocker, and thus, TTX is a promising material for the treatment of various types of pain. In this review, we will summarize the biological properties of sodium ion channel and subtype specific sodium ion channel blockers, and finally review the TTX research in pain relief.
이학섭 ( Hak Sup Lee ),김윤영 ( Yoon Young Kim ),장성수 ( Sung Su Jang ),조명수 ( Myung Soo Cho ) 서울대학교 인구의학연구소 2010 人口醫學硏究論集 Vol.23 No.-
Neural stem/progenitor cells can be differentiated or derived from various sources including pluripotent stem cells, feral brain and adult brain. Furthermore, properties of neural stem/progenitor cells can be maintained for prolonged period in vitro, and these cells are promising tools for understanding brain development and adult neurogenesis as well as cell replacement of damaged neural cells in neurodegenerative disorders. Here, we review the biological properties and culture methods of neural stem/progenitor cells derived from different sources and briefly introduce examples of clinical trials using neural stem cells.
조명수 ( Myung Soo Cho ),이학섭 ( Hak Sup Lee ),최영민 ( Young Min Choi ) 서울대학교 인구의학연구소 2010 人口醫學硏究論集 Vol.23 No.-
In people aged 65 years or older, approximately 50% of them are suffering from some degree of hearing loss. However, this disability is not restricted to the elderly. Hearing loss is mostly the result of degeneration of inner ear cells such as cochlea hair cells and sensory neurons, and incurable because loss of the cells is irreversible as of now. Currently, hearing aids and cochlear implants are available for treatment of hearing loss. But, they are not completely to cover various patterns of hearing loss. Recently, cell replacement therapies using stem cells hold the promise for a cure of hearing loss. In this review, we will discuss the hearing loss, ear structure and introduce current insights of research in cell replacement therapy for treatment of the hearing loss.
Stem Cell Based Strategies for the Treatment of Degenerative Retinal Diseases
박정현,구승엽,조명수,이학섭,최영민,문신용,유형곤,Park, Jung-Hyun,Ku, Seung-Yup,Cho, Myung-Soo,Lee, Hak-Sup,Choi, Young-Min,Moon, Shin-Yong,Yu, Hyeong-Gon The Korean Society for Reproductive Medicine 2010 Clinical and Experimental Reproductive Medicine Vol.37 No.3
망막 질환에서의 줄기세포 치료는 이전까지 치료가 불가능하다고 여겨졌던 환자들에서 시력을 향상시킬 수 있는 가능성 때문에 주목 받고 있다. 본문에서는 망막 전구세포의 분화를 위해 사용되는 태아 줄기세포, 배아줄기세포 및 성체줄기세포 등 다양한 세포 종류와, 내인적, 외인적 인자 및 이식 방법에 대해 논의하였다. 망막색소상피세포뿐만 아니라 시각세포 전구체로 성공적으로 분화시킨 실험적 연구가 보고되고 있다. 줄기세포기반치료는 아직 한계가 있지만 망막 질환 환자에서 시력을 회복하기 위한 보다 근본적인 치료 방법으로 기대되고 있다. Stem-cell therapy has the potential to improve vision in patients with untreatable retinal disease. Various types of cell source including fetal, embryonic and adult stem cells, intrinsic and extrinsic factors for differentiation into retinal progenitors and transplantation mode were discussed in this review. Experimental approaches have successfully induced photoreceptor precursor cells and retinal pigment epithelium. Stem-cell-based therapy is a promising treatment to restore vision in patients with retinal disease, in spite of the challenges.