http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
HL-60 세포에서 $TNF-{\alpha}$에 의한 MCP-1 발현에 미치는 Betulinic Acid의 효과
김경찬,이추희,Kim, Kyung-Chan,Lee, Chu-Hee 대한약학회 2008 약학회지 Vol.52 No.1
Betulinic acid, a naturally occurring pentacyclic triterpenoid, is found in abundance in the outer bark of white birch (Betula alba). In this study, we investigated if betulinic acid affects cytokine expression from activated macrophage cells. ELISA result showed that stimulation of HL-60 cells with proinflammatory cytokine such as $TNF-{\alpha}$ resulted in MCP-1 release into culture medium. In addition, transcriptional upregulation of MCP-1 in response to $TNF-{\alpha}$ was observed by RT-PCR analysis. However, incubation of HL-60 cells with betulinic acid prior to $TNF-{\alpha}$ treatment abrogated MCP-1 expression in transcription and translational level. Consistent with a number of studies which reported requirement of ERK activation for $TNF-{\alpha}$ expression, Western blot analysis showed that $TNF-{\alpha}-induced$ ERK activation was suppressed by pretreatment of HL-60 cells with betulinic acid. Taken together, our data indicate that betulinic acid exerts its anti-inflammatory effect through inhibition of $TNF-{\alpha}-induced$ ERK activation which is required for the subsequent MCP-1 release.
연구논문 : 생명과학 ; 대장균에서 발현시킨 글리포제이트 내성 콩의 5-enolpyruvylshikimate-3-phosphate 합성 효소는 거의 앨러지원성이 없음
장현성 ( Hyun Sung Chang ),김남희 ( Nam Hee Kim ),박명진 ( Myong Jin Park ),임시규 ( Si Kyu Lim ),김성철 ( Sung Chull Kim ),김지영 ( Ji Young Kim ),김정애 ( Jung Ae Kim ),오혜영 ( Hye Young Oh ),이추희 ( Chu Hee Lee ),허근 ( Keun 영남대학교 약품개발연구소 2003 영남대학교 약품개발연구소 연구업적집 Vol.13 No.-
Lee, Chu Hee,Bang, Jeong Hee,Nam, Doo Hyun 영남대학교 약품개발연구소 1993 영남대학교 약품개발연구소 연구업적집 Vol.3 No.-
Saccharomyces cerevisiae와 Kluyveromyces fragilis의 세포융합을 통해 얻은 융합효모를 calcium alginate bead에 고정화시킨 후 유당으로부터의 에탄올 생산을 시도하였다. 우선 융합효모의 에탄올 내성을 조사한 결과, 모 효모인 K. fragilis 보다 높은 8.0%에서 최대 내성을 나타내었다. 이렇게 고정화시킨 융합효모를 충전식 반응기에 충전하고 에탄올 생산을 실시하였는데, 10% 유당을 공급하고 30℃에서 운전하였을 때 가장 좋은 에탄올 생산능을 보여주었다. 특히 반응기의 희석률이 0.76hr^(-1) 일 때 13.3 g/l/hr의 높은 에탄올 생산능을 얻을 수 있었다. Yeast cells of a fusant strain constructed by protoplast fusion of Saccharomyces cerevisiae and Kluyveromyces fragilis were immobilized on calcium alginate beads. The increment of the ethanol tolerance of this strain to 8.0%, when compared with the parent K. fragilis, was confirmed. Based on the results from jar fermentation, a packed-bed reactor of the immobilized yeast cells was operated. The optimal performance of the immobilized yeast reactor for ethanol production was achieved when supplying 10% lactose (supplemented 1.0% yeast extract) at a temperature of 30℃. The maximal ethanol productivity was obtained as 13.3 g/l/hr at a dilution rate of 0.76 hr^(-1).
LEE, CHU HEE,YANG, SUN A,RHO, JU WON,LEE, SEUNG YUP,NAM, DOO HYUN 영남대학교 약품개발연구소 1992 영남대학교 약품개발연구소 연구업적집 Vol.2 No.-
The fermentative characteristics in ethanol production from lactose, with increased ethanol tolerance, of a fusant yeast strain constructed by protoplast fusion of Saccharomyces cerevisiae and Kluyveromyces fragilis were studied. The ethanol tolerance of this strain was increased to 8.0%, compared with the parent K. fragilis. During batch ethanol fermentation the optimal cultivation conditions for this fusant yeast were an initial pH of 4.5, a culture temperature 30℃, stirring at 100 rpm without aeration in 10% lactose medium (supplied with 1.0% yeast extract). Using this fusant strain in whey fermentation to ethanol, maximum ethanol production reached 3.41% (w/v) (theoretical yield; 66.7%) after a 48 hour cultivation period.
Molecular Cloning of Xanthine Oxidase Gene from Mouse Liver cDNA Library
Lee, Chu Hee,Nam, Doo H.,Huh, Keun 영남대학교 자원문제연구소 1994 資源問題硏究 Vol.13 No.-
Usirn antisera against boyine xanthine oxidase(E. C.1.1.3.22) raiaed by subcutaneous injection in the back of rabbits, the recomniant phages of mouse liver CDNA library in λgt11 were selected throughout the immunochemical method. To validate the selected clones. the recombinant phages were Iysogenized in E. coli Y1099 and their Iysates were analysed for enzyme activity and immunoreactivity. It was verified that lysates of the Iysogens not only exhibited the enzymatic activity but also bound with anti-(xanthine oxidase) antibody when the gene was induced by isopropyl-β-D-thiogalactopyranoside ( IPTG ) .
An Active Monomeric Form of Bovine Milk Xanthiine Oxidase
Lee, Chu Hee,Nam, Doo Hyun,Huh, Keun 영남대학교 약품개발연구소 1995 영남대학교 약품개발연구소 연구업적집 Vol.5 No.-
Upon gel filtration, the commercial bovine milk xanthine oxidase preparation was fractionated into two preparations showing enzyme activity. Native polacrylamide gel electrophoresis showed that one was in a dimeric form and the other was a monomer having molecular weight of 150 kDa. It was also found that this commercial enzyme existed mostly in an active monomeric form without loss of enzyme activity. The rabbit antisera produced against two enzyme preparations cross-reacted well each other. In SDS-polyacrylarnide gel electrophoresis, however, both enzyme preparations yielded two smaller protein bands below 150 kDa, which appeared to bind with both antisera with high affinity but not to retain enzyme activity. It implies that bovine milk xanthine oxidase can lose its activity when monomeric subunit is further degraded.