비글견을 이용한 GST 추출물의 단회 경구투여 용량증가 독성시험

이철화,양원경,정인철,진미림,김승형,박양춘,Lee, Chul-wha,Yang, Won-kyung,Jung, In-chul,Jin, Mi-rim,Kim, Seung-hyeong,Park, Yang-chun 대한한방내과학회 2016 大韓韓方內科學會誌 Vol.37 No.1

Objectives: To provide information on the safety of GST (Gami-Sasangja-tang), we carried out a single oral dose-increasing toxicity test of GST in beagle dogs.Materials and Methods: Six beagle dogs (three males and three females) were randomly assigned to two groups (experimental group: n=4, control group: n=2). The experimental group (two males, two females) was given oral doses of GST in increasing order (1,250, 2,500, and 5,000 mg/kg) at three-day intervals. After administration, the participants’ mortality, clinical signs, and body weight changes were monitored for two weeks. After two weeks, all dogs were sacrificed for autopsy.Results: Temporary vomiting was observed according to increasing dosage (n=1, 250 mg/kg; n=4, 2,500 and 5,000 mg/kg). Transient diarrhea was observed on the second and third dosing day (n=1, 2,500 mg/kg; n=2, 5,000 mg/kg). Temporary salivation was noted on the third dosing day (n=3, 5,000 mg/kg). Compound-colored stool was observed in all dogs fed the GST on all dosing days and also on the following days. We found no mortality and no abnormalities in the clinical signs, body weight, and gross findings in any of the dogs tested.Conclusions: The maximum tolerated dose was over 5,000 mg/kg for both male and female dogs.

만성폐쇄성폐질환 및 미세먼지 유발 폐손상 동물모델에서 과루행련환의 효과

이철화,양원경,유이란,김승형,박양춘,Lee, Chul-wha,Yang, Won-kyung,Lyu, Yee-ran,Kim, Seung-hyeong,Park, Yang-chun 대한한방내과학회 2017 大韓韓方內科學會誌 Vol.38 No.3

Objective: This study aimed to use a mouse model to evaluate the effects of Gwaruhaengryeon-hwan (GHH) on chronic obstructive pulmonary disease (COPD) and particulate matter induced lung injury. Materials and Methods: The study was carried out in two ways (in vitro, in vivo). In vitro RAW 264.7 cells (mouse macrophage) were used and analyzed by flow cytometry, ELISA. In vivo lipopolysaccharide (LPS) and cigarette smoke solution (CSS), or coal, fly ash, diesel exhaust particle (CFD) challenged mice were used and its BALF was analyzed by ELISA, lung tissue by real-time PCR. Results: In vitro, GHH maintained an 80-100% rate of viability. So cytotoxicity was not shown. In the ELISA analysis with RAW 264.7 cells, GHH significantly decreased NO over $30{\mu}g/ml$. In the ELISA analysis, GHH significantly decreased $TNF-{\alpha}$, IL-6 over $300{\mu}g/ml$. In the COPD model, the GHH 200 mg/kg dosage group, the application of GHH significantly decreased the increasing of neutrophils, $TNF-{\alpha}$, IL-17A, MIP2, CXCL-1 in BALF, $TNF-{\alpha}$, $IL-1{\beta}$ mRNA expression in lung tissue and histological lung injury. In the CFD induced lung injury model, the GHH 200 mg/kg dosage group, the application of GHH significantly decreased the increase of neutrophils, $TNF-{\alpha}$, IL-17A, MIP2, CXCL-1 in BALF, MUC5AC, $TGF-{\beta}$ mRNA expression in lung tissue and histological lung injury. Conclusion: This study suggests the usability of GHH for COPD patients by controlling lung tissue injury.

GST 추출물의 유전독성평가

이철화(Chul Wha Lee),한종민(Jong Min Han),이미영(Mi Young Lee),정인철(In Chul Jung),진미림(Mirim Jin),김승형(Seung Hyung Kim),박양춘(Yang Chun Park) 한의병리학회 2014 동의생리병리학회지 Vol.28 No.6

배드민턴 동호인의 사회적 지지와 운동몰입 및 여가만족의 구조적 관계

안수운(Soo Woon Ahn),이철화(Chul Wha Lee) 한국사회체육학회 2013 한국사회체육학회지 Vol.0 No.53

The purpose of this study was to find out structural relationships among social support, exercise commitment and leisure satisfaction of badminton participants. For the study the 241 subjects were collected, who take part in badminton club. Social support, exercise commitment and leisure satisfaction scale were used for data collection of this study. The statistical methods used for this study are exploratory factor analysis, confirmatory factor analysis, structure equation modeling analysis. The conclusion of this study are as follows: First, social support of the badminton participants had significant effect on exercise commitment. Second, social support of the badminton participants had not effect on leisure satisfaction. Third, exercise commitment of the badminton participants had significant effect on leisure satisfaction of badminton participants.

중·고등학교 운동선수의 사회적 지지가 문제해결 능력에 미치는 영향

안수운(Ahn Soo Woon),이철화(Lee Chul Wha) 학습자중심교과교육학회 2015 학습자중심교과교육연구 Vol.15 No.12

본 연구는 중․고등학교 운동선수의 사회적 지지와 사회적 문제해결 능력과의 관 계를 규명하기 위하여 인구통계학적 변인에 따른 사회적 지지와 문제해결 능력을 알 아보고, 사회적 지지에 따른 사회적 문제해결 능력의 정도를 측정하여 그 차이를 조 사 분석함으로써 중․고등학교 운동선수의 올바른 가치관 형성과 지도 육성에 기초 자료를 제공하고자 한다. 이러한 연구의 목적을 달성하기 위하여 C도와 D광역시에 거주하고 있는 중․고등 학교 운동선수를 모집단으로 설정하고 비확률적 표본추출방법의 하나인 할당표집으 로 350명을 표집 하였다. 설문지의 타당성 확보를 위해 탐색적 요인분석(exploratory factor analysis: EFA)을 이용하여 타당도 검사를 실시하였고, 수집된 자료는 SPSS 18.0 통계 프로그램을 이용하여 t-test, 일원분산분석, 상관관계분석, 다중회귀분석을 실시하였다. 연구결과, 첫째, 인구통계학적 특성에 따른 중․고등학교 운동선수의 사회적 지지에 서 성별, 학교급별, 운동경력, 경기유형, 입상실적에서 차이가 나타났다. 둘째, 인구통계 학적 특성에 따른 중․고등학교 운동선수의 문제해결 능력에서 학교급별, 운동경력, 경 기유형, 입상실적에서 차이가 나타났다. 셋째, 사회적 지지에 따라 문제해결 능력에 영 향을 미치는 것으로 나타났다. 평가적 지지는 문제해결 능력의 3개요인 모두에 영향을 미치는 것으로 나타났고, 정보적, 물질적 지지는 인지, 행동척도에 영향을 미치는 것으 로 나타났으며 정서적 지지는 인지 척도에 영향을 미치는 것으로 나타났다. This study classified the social support into emotional, evaluative, informative and material support and the problem-solving ability into cognition scale, emotional scale and behavior scale in order to identify the relations between social support and problem-solving ability. Based on this, this study is aimed at providing basic material for the formation of correct value system and fostering of middle and high school athletes by investigating the social support and problem-solving ability according to demographic variables and measuring the degree of social problem-solving capacity in accordance with social support. To achieve the goal, a population was set with middle and high school athletes in C Province, D City. Among them, 350 students were selected in quota sampling which is one of Non-Probability Sampling Methods. The statistical methods used for this study are exploratory factor analysis, t-test, one-way ANOVA, Correlation Analysis and Multiple Linear Regression by the use of SPSS 18.0 statistics program. The conclusion of this study are as follows: First, It was investigated that the factors affecting social support of middle and high school athletes according to demographic characterization were gender, school grade, exercise period, game type, winning a prize performance. Second, It was investigated that the factors affecting problem-solving ability of middle and high school athletes according to demographic characterization were school grade, exercise period, game type, winning a prize performance. Third, social support made the significant impact on the formation of problem-solving ability. It was identified that evaluative support influenced all three variables of problem-solving ability. It was found that the informative support and material support influenced cognition scale and behavior scale. In addition, it was investigated that emotional support influenced cognition scale.

초등학생의 체육수업만족과 신체상 및 미래 삶 기대의 구조적 관계

김수정(Kim, Soo-jung),이철화(Lee, Chul-Wha),안수운(Ahn, Soo-Woon) 학습자중심교과교육학회 2014 학습자중심교과교육연구 Vol.14 No.3

본 연구는 초등학생들의 미래 삶의 기대를 향상시키기 위한 접근으로 체육수업만족과 신체상 및 미래 삶의 기대에 대한 인과관계를 분석하여 초등학생의 미래 삶의 기대를 향상시킬 수 있는 프로그램으로서 체육수업만족의 역할과 가능성에 대한 이 론적 근거를 마련하는데 그 목적이 있다. 이러한 연구의 목적을 달성하기 위하여 경기, 인천, 충북, 충남에 거주하고 있는 초등학생을 모집단으로 설정하고 비확률적 표본추출방법((Non-Probability Sampling Methods)의 하나인 할당표집(quota sampling)으로 450명을 표집하여, 설문에 응답하지 않거나 불성실한 응답자를 제외한 후 총 429명의 유효 표본을 확보한 후 자료를 분석하였다. 자료 수집을 위한 연구도구로는 선행연구에서 사용된 문항을 기초로 하여 이 연구에 맞게 수정 보완하여 사용하였으며, 설문지의 타당성 확보를 위해 탐색적 요인분석(exploratory factor analysis : EFA)과 확인적 요인분석(confirmatary factor analysis : CFA)을 이용하여 타당도 검사를 실시하였고, 자료 분석을 위하여 본 연구에서 사용한 통계분석기법은 구조방정식(structure equation modeling)을 실시하였다. 연구결과, 첫째, 초등학생의 체육수업만족은 신체상에 유의한 영향을 미친다. 둘째, 초등학생의 체육수업만족은 미래 삶의 기대에 유의한 영향을 미치지 않는다. 셋째, 초등학생의 신체상은 미래 삶의 기대에 유의한 영향을 미친다. The purpose of the study was to prepare theoretical ground about a role and possibility of physical class satisfaction as a program to improve the future life expectation of elementary school students by analyzing cause and effect relationship about physical class satisfaction, body image, and the future life expectation. To achieve the goal, a population was set with elementary school students in Gyeonggi, Incheon, Chungbuk, Chungnam. Among them, 450 students were selected in quota sampling which is one of Non-Probability Sampling Methods. Finally, effective samples of 429 in total were secured for analysis, excluding non-respondents or insincere respondents. For study tool for collecting data, question items used in existing studies were revised and supplemented fit to the study. The statistical methods used for this study are exploratory factor analysis, confirmatory factor analysis, structure equation modeling analysis. The conclusion of this study are as follows: First, physical class satisfaction of elementary school had significant effect on body image. Second, physical class satisfaction of elementary school had not effect on the future life expectation. Third, body image of elementary school had significant effect on the future life expectation.