개 심장사상충(Dirofilaria immitis) 진단을 위한 항원성 조사 및 단크론항체 생산

이철순,지차호,Lee, Cheol-soon,Jee, Cha-ho 대한수의학회 2000 大韓獸醫學會誌 Vol.40 No.1

In order to diagnose canine heartworm infection by antigen capture ELISA, the crude somatic(S), partial somatic(below 45kDa) and excretory/secretory(E/S) antigen of adult heartworm were identified and the antigenicity was examined by silver stain, immunoblot and ELISA. Then, production of monoclonal antibody to specific antigen carried out in this experiment. The bands to S antigen and E/S antigen were recognized between 10 and 200kDa and common bands were recognized strongly 14, 18, 28, 43kDa by silver stain. By western blot analysis, fractions to S antigen were recognized 14, 16, 18, 20, 24, 28, 32, 43, 50, 55kDa, etc. and only a 14kDa to E/S antigen in positive sera which were positive in modified Knott's test and necropsy. In ELISA, the positive sera reacted to antigens(SA, $SA_{45}$, E/S) were significantly different from negative sera by Student's t-test(p<0.05). Four hybridoma cell lines(14, 16, 17, 32kDa) than produce specific monoclonal antibodies for these antigens were obtained by immunizing BALB/c mice with a partially purified somatic antigen (below 45kDa) preparation, by fusing spleen cells with SP2/O cell myeloma cells, and by screening cell culture supernatants for antibody. In these results, it was confirmed that partial somatic antigen(below 45kDa) or E/S antigen can be used for serologic diagnosis of heartworm infection and monoclonal antibody reacting with specific antigen(14kDa) can be used for antigen capture ELISA in prepatent period of canine heartworm infection.

한국인 집단에서 알코올 의존과 세로토닌 수송체 유전자 다형성의 연관성

이철순(Cheol-Soon Lee),노양덕(Yang-Duck Noh),구준(Joon Gu),박철수(Chul-Soo Park),손진욱(Jin-Wook Shon),한규희(Kyu-Hee Hahn),김봉조(Bong-Jo Kim) 대한생물치료정신의학회 2007 생물치료정신의학 Vol.13 No.2

Objective:Family, twin, and adoption studies have demonstrated that genes play an important role in the development of alcoholism. We investigated the association between alcoholism and the genetic polymorphisms of the serotonin transporter regulatory gene(5-HTTLPR) in Korean population. We also explored the association between the genetic polymorphisms of the 5-HTTLPR and clinical characteristics in patients with alcohol dependence. Methods:The genotype and allele frequencies of the 5-HTTLPR were investigated in 172 control subjects and 162 male hospitalized patients who met Diagnostic and Statistical Manual of Mental Disorders, Fourth Edition(DSMIV), criteria for alcohol dependence. Several standardized research scales were used for the clinical assessment of alcoholism, including the Alcohol Dependence Scale(ADS), the Beck Depression Inventory, the Beck Anxiety Inventory, and the Obsessive-Compulsive Drinking Scale. Results:We found that the frequency of LL genotype and L allele was higher in patients with alcohol dependence than that of control subjects. Alcoholics with the 5-HTTLPR LS or SS genotypes had a later age of onset (mean age of onset, 29.4 and 32.1 years, respectively) than those with the LL genotype(mean age of onset, 25.9 years;p=.002). Also we found that the polymorphisms of the 5-HTTLPR were associated with the scores of the ADS, but not associated with the scores of the BDI, BAI, and OCDS. Alcoholics with the L allele had an earlier age of onset and higher scores of the BDI than those with the S allele(respectively p=.019, p=.021). The scores of the ADS were higher in patients with the S allele than those with the L allele(p=.002). Conclusion:Our finding suggest that genetic polymorphisms of the 5-HTTLPR may be associated with the development of alcoholism and that the 5-HTTLPR play an important role in the development of the early onset and the severe type of alcoholism.

항공기 올레오식 2중 완충기 종류에 따른 특성 비교 연구

이철순(Cheol Soon Lee),정선호(Seon Ho Jeong),김경종(Kyung Jong Kim),김정호(Jeong Ho Kim),조진연(Jin Yeon Cho) 한국항공우주학회 2017 韓國航空宇宙學會誌 Vol.45 No.11

본 연구에서는 세 가지 종류의 올레오식 2중 완충기 특성 비교를 위한 첫 단계 연구로서 수학적 모델링을 새롭게 수행하였다. 논문의 완결성을 위해 먼저 전형적인 올레오식 단일 완충기에 대한 모델을 제시한 후, 세 가지 종류의 올레오식 2중 완충기에 대한 수학적 모델을 유도하였다. 수학적 모델링을 위해 베르누이 방정식과 오리피스 방출계수를 이용하였으며, 완충기 내부의 기체와 유체는 각각 폴리트로픽 과정 그리고 비압축성으로 가정하였다. 올레오식 2중 완충기 특성 연구에 제안된 모델을 적극 활용할 수 있을 것으로 판단된다. In this work, mathematical models are newly presented for three types of double-acting oleo-pneumatic shock absorbers as the first part of a comparative study on the several types of double-acting oleo-pneumatic shock absorbers. After a typical single-acting shock absorber model is presented for the sake of completeness, mathematical models of three types of double-acting shock absorbers are proposed. To derive the models, Bernoulli equation and orifice discharge coefficient are utilized along with the assumptions of incompressibility and poly-tropic process. The proposed models are expected to be used for investigation of the salient features of several types of double-acting oleo-pneumatic shock absorbers.

한국인 집단에서 알코올 의존과 Tryptophan Hydroxylase A218C 유전자 다형성의 연관성

이철순(Cheol-Soon Lee),박철수(Chul-Soo Park),이환철(Hwan-Chul Lee),구준(Joon Gu),손진욱(Jin-Wook Sohn),한규희(Gyu-Hee Hahn),윤혁진(Hyuck-Jin Yoon),김봉조(Bong-Jo Kim) 대한생물치료정신의학회 2006 생물치료정신의학 Vol.12 No.1

목적 : 알코올 의존은 환경적 요인과 생물학적 요인이 복합되어 발생하는 것으로 널리 알려진 질환으로 최근 가족, 쌍생아 및 양자 연구에서 유전적 요인의 중요성이 시사되고 있다. 본 연구는 알코올 의존의 유전적 요인을 찾기 위해 세로토닌 합성 조절 효소인 tryptophan hydroxylase(TPH) A218C 유전자 다형성 빈도를 정상 대조군과 비교하였으며 알코올 의존 환자에서 TPH A218C의 유전자 다형성과 임상적 변인과의 관련성을 알아보고자 하였다. 방법 : DSM-Ⅳ 진단 기준에 부합되는 알코올 의존 환자 162명과 정상 대조군 182명을 대상으로 TPH 유전자 분석을 시행하였다. 알코올 의존 환자에서 TPH 유전자 다형성과 임상적 변인간의 관련성을 알아보기 위해서 알코올 의존 환자들을 대상으로 알코올 의존 척도, 불안 척도, 우울 척도, 강박적 음주 척도를 조사하였다. 결과 : 알코올 의존 환자군에서 정상 대조군에 비하여 AA 유전자형과 A 대립 유전자의 빈도가 높게 나타났다. 유전자형에 따른 평균 발병 연령을 조사한 결과 AA, AC, CC에서 각각 25.8세, 30.5세, 33.6세로 조사되어 AA 유전자형을 가진 환자군에서 유의하게 낮은 발병 연령을 보였다(p<0.001). 또한 AA 유전자형을 가지는 환자군에서 CC 유전자형을 보인 환자군에 비하여 알코올 의존 척도에서 유의하게 높은 값을 가지는 것으로 나타났다(p=0.001). 결론 : TPH A218C 유전자 다형성이 알코올 의존의 원인적 요인으로 작용할 있을 뿐만 아니라 발병 연령 및 증상 심각도와 관련이 있다. 이러한 결과는 조기 발병하고 심각한 알코올 의존과 같은 알코올 의존 아형 분류의 생물학적 근거가 될 수 있다. Objective : Family, twin, and adoption studies have demonstrated that genes play an important role in the development of alcoholism. In this study, we explored the genetic causes of alcohol dependence by comparing the frequency of the tryptophan hydroxylase(TPH) A218C between patients with alcohol dependence and control subjects in Korean population. We also explored the association between TPH A218C polymorphism and clinical characteristics in patients with alcohol dependence. Method : The genotype and allele frequencies of TPH were investigated in 182 normal control and 162 male hospitalized patients who met Diagnostic and Statistical Manual of Mental Disorders, Fourth Edition, (DSM-Ⅳ) criteria for alcohol dependence. We conducted clinical assessments using Alcohol Dependence Scale(ADS), Beck Depression Inventory, Beck Anxiety Inventory, and Obsessive-Compulsive Drinking Scale. Results : The frequency of AA genotype and A allele is higher in patients with alcohol dependence than in normal control. Alcoholics with the TPH CC or AC genotypes had a later age of disease onset(mean age of onset, 33.6 and 30.5 years, respectively) than those with the AA genotype(mean age of onset, 25.8 years ; p<0.001). Also we found the TPH AA genotype has a higher score on the Korean version of the ADS than those with CC genotypes(p=0.001). Conclusion : Our results suggest that TPH polymorphism may be a crucial factor in the development of alcohol dependence and may explain, in part, the biological basis for these typologies.

한국의 일 요양 시설에 거주하는 한센병 환자들에서의 치매 유병률

이철순(Cheol Soon Lee),강효승(Hyo Seung Kang),구준(Joon Gu),박철수(Chul Soo Park),손진욱(Jin Wook Shon),김봉조(Bong Jo Kim) 대한노인정신의학회 2007 노인정신의학 Vol.11 No.2

Objectives : To identify prevalence of dementia and it's subtypes in patients with Hansen's disease in a sanatorium of Korea. Methods : The study employs a two-stage design for case identification. One hundred-six patients with Hansen's disease aged 65 and over participated in the study. The Korean Version of the Consortium to Establish a Registry for Alzheimer's disease (CERADK) assessment packet was administered to One hundred-two patients with Hansen's disease. Dementia was defined using the Diagnostic and Statistical Manual of Mental Disorders, Fourth Edition diagnostic features of dementia. Results : The prevalence was 15.1% in Hansen's sanatorium. The prevalence of Alzheimer's and vascular dementia were 10.4% and 4.7%. The age-adjusted prevalence rate for dementia was 11.9%. The rate of patients with dementia was 12.73% in males and 17.65% in females. Conclusion : The prevalence of dementia in Hansen's disease sanatorium of Korea appears to be similar with those reported in previous study. Our result suggests that Hansen's disease was unrelated to prevalence of dementia, and also that the dementia is major neuropsychiatric disease in patient with Hansen's disease.

노인에서 COVID-19로 인한 불안감과 관련된 인식

임은지(Eunji Lim),김봉조(Bong-Jo Kim),이철순(Cheol-Soon Lee),차보석(Boseok Cha),이소진(So-Jin Lee),서지영(Jiyeong Seo),최재원(Jae-Won Choi),이영지(Young-Ji Lee),이윤정(Younjung Lee),이동윤(Dongyun Lee) 대한노인정신의학회 2021 노인정신의학 Vol.25 No.1

Objective: To investigate perceptions of coronavirus disease-19 (COVID-19) associated with anxiety caused by the COVID-19 epidemic in the elderly who are vulnerable to mental health problems. Methods: This study used data of a survey on perceptions of COVID-19 and changes in mental health of 1,000 out of residents in a province of Korea in April 2020. The survey included questions about psychological perceptions for COVID-19. Subjects were dived into two groups (<60 and ≥60). Binary logistic regression analyses were performed for evaluating the association between anxiety and perceptions about COVID-19 in each group. Results: Results of binary logistic regression analyses revealed that only ‘fear of getting infected myself’among perceptions for COVID-19 was associated with anxiety in the elderly aged more than 60 years. However, in adults aged less than 60 years, all perceptions for COVID-19 except impairment of performance were associated with such anxiety. Conclusion: We found that the anxiety for COVID-19 in elderly with age over 60 years was associated with ‘fear of getting infected myself’ rather than ‘fear of family or people around them’, unlike adults aged less than 60 years. These results can be applied in strategies for psychological quarantine against COVID-19 among the elderly.

양극성 장애 입원 환자에서 비타민 D와 회복력

김창근(Chang-Geun Kim),김봉조(Bong-Jo Kim),이철순(Cheol-Soon Lee),이소진(So-Jin Lee),이동윤(Dongyun Lee),서지영(Ji-Yeong Seo),최재원(Jae-Won Choi),이영지(Young-Ji Lee),이윤정(Youn Jung Lee),임은지(Eunji Lim),차보석(Boseok Cha) 대한생물치료정신의학회 2021 생물치료정신의학 Vol.27 No.3

Objectives:The purpose of this study was to investigate the relationship between serum vitamin D concentration and resilience in patients hospitalized with bipolar disorder. Methods:Serum vitamin D levels were measured on day 1 of hospitalization in 64 patients with bipolar disorder, who were diagnosed according to the Diagnostic and Statistical Manual of Mental Disorders, 4th Edition. The Connor-Davidson Resilience Scale and the Korean version of the Inventory for Depressive Symptomatology (KIDS) were administered to these patients, and symptoms of mania were evaluated using the Young Mania Rating Scale (YMRS). Patients were then categorized into vitamin D sufficient and deficient groups based on a serum vitamin D cut-off of 20 ng/mL. Analysis of covariance (ANCOVA) was performed to compare the resilience between the two groups, and multiple linear regression was performed to determine the association between serum vitamin D levels and resilience. Results:The vitamin D deficiency group had more depressive episodes than the sufficient group (p<0.05). ANCOVA showed that the vitamin D deficient group had significantly lower resilience scores than the sufficient group (p= 0.034). Moreover, multiple regression analyses conducted by controlling age, sex, body mass index, season of blood sampling, KIDS scores, and YMRS scores, showed a significant association between serum vitamin D concentration and resilience in patients with bipolar disorder (p=0.001). Conclusions:This study suggested that vitamin D deficiency is associated with resilience as a protective factor against stress and trauma in patients with bipolar disorder. Further research is warranted to determine the causal relationship between vitamin D levels and resilience.

재난 후 소아청소년의 정신사회적 개입

이미선(Mi-Sun Lee),황준원(Jun-Won Hwang),이철순(Cheol-Soon Lee),김지연(Ji-Youn Kim),이주현(Ju-Hyun Lee),김은지(Eunji Kim),장형윤(Hyoung Yoon Chang),배승민(Seung-Min Bae),박장호(Jang-Ho Park),방수영(Soo-Young Bhang) 대한소아청소년정신의학회 2016 소아청소년정신의학 Vol.27 No.4

Objective: The aim of this systematic literature review is to analyze the psychosocial interventions for children and adolescents after disasters. Methods: We conducted a review of the extant research literature from 1991 to 2015 via a comprehensive search of the MEDLINE, EMBASE, Cochrane CENTRAL, PubMed and PsyclNFO databases. The keywords employed in this research included: ‘child’, ‘adolescent’, ‘youth’, ‘disaster’, ‘posttraumatic’, ‘psychosocial’, ‘therapy’ and ‘intervention’. The researchers followed the PRISMA guidelines. A total of 850 articles were screened for their eligibility and fifty-nine were found to meet the study criteria. The final data analysis was performed based on the disaster type, study design, type of intervention, sample size, age, school grade, number of sessions, setting of intervention delivery, providers, approach and parent involvement. Results: Countries worldwide have experienced various kinds of disasters, including earthquakes, hurricanes, vessel accidents, tornados, tsunamis, volcanic eruptions, war, fire, terrorism, and traffic accidents. The types of psychosocial intervention that were conducted after these disasters included: psychological first aid, psychological debriefing, psychoeducation, trauma focused cognitive behavior therapy, eye movement desensitization reprocessing, prolonged exposure therapy, group play therapy and arts therapy, project interventions, school-based interventions and web-based interventions. Conclusion: The findings of the systematic literature review suggest that an appropriate psychosocial intervention could be utilized as evidence-based mental health treatment for children and adolescents after disasters.

의대의학전문대학원 학생들의 삶의 질과 일주기 리듬 및 수면의 질과의 연관성

장홍경,이소진,박철수,김봉조,이철순,차보석,이동윤,서지영,Chang, Hong-Kyeung,Lee, So-Jin,Park, Chul-Soo,Kim, Bong-Jo,Lee, Cheol-Soon,Cha, Boseok,Lee, Dongyun,Seo, Ji-Yeong 대한수면의학회 2015 수면·정신생리 Vol.22 No.2

목 적 : 의학전문대학원생들은 의학교육과정 동안 심한 스트레스와 수면 부족을 경험한다. 따라서 의전원생들의 수면 양상, 불면의 정도, 일주기 리듬이 삶의 질과 어떠한 연관성이 있는지 알아보고자 하였다. 방 법 : 경상대학교 의학전문대학원(의전원) 4학년 46명을 대상으로 하였다. 병원 불안-우울 척도, 불면증 심각성 척도, 아침형-저녁형 문항과 삶의 질을 측정하기 위한 세계보건기구 삶의 질 간편형 척도를 사용하였다. 삶의 질과 연관된 요인들을 밝히기 위하여 피어슨 상관분석을 시행하였고 상관성 있는 변수들을 선정하여 다중회귀분석을 시행하였다. 결 과 : 의전원 학생들 중 13명에서 약간의 불면증 경향이 있었다. 명백한 저녁형은 3명, 중등도 저녁형은 12명, 중간형은 28명, 중등도 아침형은 3명이었으며 명백한 아침형은 없었다. 피어슨 분석에서 신체적 영역의 삶의 질과 연관을 보인 요소들은 나이, 주중 및 주말 평균 수면시간, 주중과 주말의 수면 차, 우울, 불면증의 심각성 정도, 아침형-저녁형 점수였고 심리적 영역의 삶의 질과 연관을 보인 요소들은 주중 및 주말의 평균 수면, 불안, 불면증의 정도와 아침형-저녁형 점수였다. 사회적 영역의 삶의 질에서는 성별, 주중 평균 수면시간, 우울, 불면증 점수가 연관성이 있었고, 환경적 영역의 삶의 질에서는 주중 및 주말의 평균 수면시간과 불면증의 심각성 정도가 연관성이 있었다. 다중회귀분석을 통하여 불면증의 심각도가 낮을수록 신체적 건강영역, 심리적 영역과 환경적 영역의 삶의 질이 높고, 저녁형 일수록 신체적 건강 영역과 심리적 영역의 삶의 질이 높은 것으로 나타났다. 결 론 : 의전원 학생들에서 수면의 질이 좋을수록 신체적 및 심리적, 환경적 영역의 삶의 질이 높았고, 저녁형일수록 신체적 및 심리적 영역의 삶의 질이 높았다. 이를 통해 학생들의 수면 습관 개선이 정서적 문제에 대한 개입 못지 않게 중요하다는 것을 알 수 있다. Objectives: We investigated whether sleep behaviors, severity of insomnia and circadian typology are associated with quality of life among Gyeongsang National University (GNU) medical students. Methods: Questionnaires were distributed to 46 fourth-year students of GNU Medical School. The hospital anxiety-depression scale, insomnia severity index (ISI), morningness-eveningness questionnaire (MEQ) and WHO Quality of Life-BREF (WHOQOL-BREF) were used. Results: Quality of life in terms of physical health was associated with age, weekday total sleep time (TST), weekend TST, catch up sleep, depression, ISI score, and MEQ score. Quality of life in terms of psychological health was correlated to weekday and weekend TST, anxiety, ISI score and MEQ score. Sex, weekday TST, depression, and ISI score were revealed to influence the social aspect of quality of life, and weekday and weekend TST, and ISI score were associated with quality of life in terms of environment. Multiple regression analysis showed that better sleep quality predicted to a higher quality of life in terms of physical, psychological and environmental health, and eveningness was associated with a better quality of life in terms of physical and psychological health. Conclusion: It is concluded that physical, psychological, and environmental aspects of quality of life were associated with quality of sleep and that physical and psychological aspects of quality of life were related to eveningness in GNU medical students. Therefore, it is equally important to treat emotional problem as well as improve sleep quality.

암 환자의 정신병리와 치료 목적에 대한 인지 및 삶의 질의 관계

이다원(Da Won Lee),이소진(So Jin Lee),김승남(Seung Nam Kim),김봉조(Bong Jo Kim),이철순(Cheol Soon Lee),차보석(Bo Seok Cha),박철수(Chul Soo Park),손진욱(Jin Wook Shon) 대한생물치료정신의학회 2011 생물치료정신의학 Vol.17 No.1

Objectives : We evaluated the relationship of the psychopathology, quality of life and perception of treatment goal in patients with cancer. Methods : A sample of 102 cancer patients completed both Hospital Anxiety and Depression Scale(HADS) and European Organization for Research and Treatment of Cancer Quality of Life Questionnaire C-30(EORTC QLQ C-30). We compared the differences of the psychopathology of patients according to perception of treatment goal by chi-square test and evaluated risk factors for palliative perception of treatment goal by multiple logistic regression. We exhibited t-test to compare HADS and EORTC QLQ C-30 and the association between HADS and EORTC QLQ C-30 was analyzed by Pearson correlation analysis. Results : Of 102, 25 cancer patients had the psychopathology(HAD-T ≥19), including 21 for anxiety(HAD-A ≥8), 59 for depression(HAD-D ≥8). The association between HADS and EORTC QLQ C-30 was negatively correlated with statistical significance. Patients with depression and severe psychopathology showed statistically significant tendency to perceive treatment goal as palliative. Depression and metastasis were risk factors for palliative perception of treatment goal. Conclusions : Psychopathology and quality of life in patients with cancer is important for perception of treatment goal. Assessment and intervention to improve psychological distress and quality of life is warranted during all periods of cancer treatment.