방사성(放射性) 인(燐) (³²P)이 계태아(鷄胎兒) 및 초생추발육(初生雛發育)에 미치는 영향(影響)

이준섭,윤석봉,고광두,Lee, Joon Sup,Yoon, Suk Bong,Ko, Kwang Doo 대한수의학회 1971 大韓獸醫學會誌 Vol.11 No.2

This experiment was performed to study the effects of radioactive phosphorus($^{32}p$) on the growth of chick embryo and young chick. Radioactive phosphorus($^{32}p$) was administered into the yolk sac of chick embryo in doses of 2 uci/gm and 1 uci/gm and was administered intraperitoneally to the young chick in doses of 1 uci/gm and 0.5 uci/gm. The chick embryos were sacrificed on 6th, 7th, 8th, 9th and 10th day and the chicks were sacrificed on 1st, 3rd, 6th and 10th day after the administration and the liver, kidney, spleen, brain, eye ball and femur were weighed to observe the effects of growth inhibition on them. The results obtained were as follows. 1. A marked growth inhibitory effect was found on 8th, 9th and 10th day after the administration of $^{32}p$ in chick embryos and the same effect was found on 6 th and 10 th day after the administration in chicks. 2. The growth of the liver, kidney, spleen and femur was inhibited markedly but the brain and eye ball were not affected in chick embryos and chicks.

Studies on Toxicity of Ngaione in Rats

이준섭,Lee, Joon Sup The Korean Society of Veterinary Science 1979 大韓獸醫學會誌 Vol.19 No.2

Myoporum deserti에서 얻어지는 유독성식물류(有毒性植物油)인 ngaione을 체중(體重) 90~110g의 Wistar계(系) 웅성백서(雄性白鼠)에 복강내(腹腔內)로 주입(注入)한 다음 유독성(有毒性)을 조사(調査)하여 다음과 같은 결과(結果)를 얻었다. 흰쥐에서의 ngaione의 치사량(致死量) ($LD_{50}$)은 체중(體重) kg당(當) 166mg 이었고 95% 신뢰한계(信賴限界)는 체중(體重) kg당(當) 96~287mg이었다. Ngaione 주입후(注入後) 간장(肝臟)에서 언제나 병변(病變)이 관찰(觀察)되었으며 이들의 병리조직학(病理組織學)적 소견(所見)은 소엽중간부괴사(小葉中間部壞死)(midzonal necrosis)였다. Naaione의 주입양(注入量)이 많은 동물(動物)에서는 자주 위벽(胃壁)(점막하직(粘膜下織))과 대장(大腸)의 일부(一部)에서 산만성출혈(散漫性出血)을 동반(同伴)하고 있었다.

Humboldt 종(種) Penguin(Stheniscus humboldti)의 건낙성기낭염(乾酪性氣囊炎)을 동반(同伴)한 기관지염(氣管枝炎)과 고환(睾丸)의 Melanin 색소(色素) 심착증(沈着症)

이준섭,임창형,오순섭,오창영,Lee, Joon Sup,Lim, Chang Hyeong,Oh, Soon Sup,Oh, Chang Young 대한수의학회 1975 大韓獸醫學會誌 Vol.15 No.2

Four Humboldt penguins were imported from overseas in March, 1975. Soon after arrival at the Zoological Garden of Chang Gyeong Won in Seoul, two penguins became ill with symptoms of severe dysnnoea, vomiting, diarrhea and mild convulsion. They died on the second day of illness. The main pathological finding was bronchitis which accompanied a caseous airsacculitis on the left lung. A massive melanin pigmentation in the intertubular tissue of the testis was found accidentally.

Effect of Dietary Protein on Toxicity and Liver Lesion Caused by a Single Intraperitoneal Dose of Ngaione in Rats

이준섭,Lee, Joon Sup The Korean Society of Veterinary Science 1979 大韓獸醫學會誌 Vol.19 No.2

실험동물(實驗動物) 사료(飼料)중의 단백질함량(蛋白質含量)이 ngaione에 중독(中毒)된 흰쥐의 독성(毒性) 및 간장병변(肝臟病變)에 미치는 영향(影響)을 관찰(觀察)하였던 바 그 결과(結果)는 다음과 같다. 사료중(飼料中)의 단백질함량(蛋白質含量)을 0, 15 및 30%로 맞추어서 3개(個)의 실험군(實驗群)에 각각(各各) 10일간(日間)씩 급여(給與)한 다음 이들 동물(動物)에 ngaione을 간장내(腹腔內)로 투여(投與)하였으나 이에 의(依)한 독성(毒性)의 변화(變化)는 없었다. 단백질(蛋白質) 사료(飼料)를 10일간(日間) 급여(結與)하지 않은 동물(動物)의 hepatic microsomal mixed function oxidases (HMFO)의 기능(機能)은 단백질사료(蛋白質飼料)(15 및 30%)를 급여(給與)한 동물(動物)에서보다 약(約) 50% 감소(減少)되었고, 수면시간(睡眠時間)은 약(約) 5배(倍)로 연장(延長)되었다. ngaione에 의(依)해서 야기(惹起)된 간장병변부위(肝臟病變部位)는 0 및 30% 단백질(蛋白質) 급여군(給與群)에서 각각(各各) 소엽중심부(小葉中心部)(centrolobular region)와 백관주위부(脈管周圍部)(periportal region)에서 관찰(觀察)되었으나 15% 단백질(蛋白質) 급여군(給與群)에서는 ngaione의 투여량(投與量)에 따라서 소엽중심부(小葉中心部), 맥관주위부(脈管周圍部) 및 소엽중심부(小葉中間部)(midzonal region)중(中)에서 어느 부위(部位)에서나 관찰(觀察)되었다.

Sodium Phenobarbitone 및 β-Diethylaminoethyl Diphenylpropyl Acetate (SKF 525 A)가 Ngaione에 중독(中毒)된 흰쥐의 간장병변(肝臟病變)에 미치는 영향(影響)

이준섭,Lee, Joon Sup 대한수의학회 1974 大韓獸醫學會誌 Vol.14 No.2

Ngaione isolated from leaves of Myoporum deserti was dosed to the phenbarbitone and SKF 525 A pretreated male rat and studied the liver lesions. The results obtained were summarized as follows: 1. The liver lesions are mostly zonally distributed and involved the midzonal parenchyma chiefly with tendence to include also associated periportal hepatocytes. 2. The histopathology of liver due to ngaione after phenoharbitone pretreatment is characterized by the consistent pretence of degeneration and necrosis of the periportal parenchyma. 3. Zonal liver lesions caused by ngaione in the SKF 525 A pretreated rat are consistently periacinar in location.

닭의 Leucocytozoon병(病)의 발생(發生)

이준섭,Lee, Joon Sup 대한수의학회 1974 大韓獸醫學會誌 Vol.14 No.2

This investigation was performed to study of chickens naturally infected with leucocytozoon. The results obtained were summarized as follows: 1. The characteristic macroscopical changes observed were hemorrhages and elevated greyish white spots on the oviduct, the liver, the pancreas, the kidney and the pectoral muscles. 2. Microscopically, megaloschizonts were located in groups in the elevated greyish white spots. Hemorrhage and degenerative necrotic changes were observed around the megaloschizonts.

항산화제가 bleomycin에 의해서 유발되는 랫트 폐장병변에 미치는 영향에 관한 연구

이준섭,김대중,윤여성,Lee, Joon-sup,Kim, Dae-joong,Yoon, Yeo-sung 대한수의학회 1991 大韓獸醫學會誌 Vol.31 No.4

The purpose of this study was to investigate the effect of antioxidants (vitamin E, selenium, and coenzyme $Q_{10}$) on the bleomycin-induced pulmonary lesions in male rats. Sprague-Dawley male rats were divided into 4 treatment groups ($T_1$, $T_2$, $T_3$, $T_4$) and 4 control groups ($C_1$, $C_1$, $C_3$, $C_4$). The treatment groups of rats weie given a single intratracheal dose of bleomycin (1.5 units/rat) and control groups of rats were given a single intratracheal dose of normal saline (0.15ml/rat). The rats in the $T_1$ group and $C_1$, group were dosed with normal saline (0.5ml/kg/day), the rats in the $T_2$ group and $C_2$ group were dosed with vitamin E (50mg/kg/day), the rats in the $T_3$ group and $C_3$ group were dosed with sodium selenite (3mg/kg/day) and the rats in the $T_4$ gronp and $C_4$ group were dosed with coenzyme $Q_{10}$ (2.5mg/kg/day) intraperitoneally for 7 days or 14 days, respectively. Animals were killed at 7th and 14th day after dosing with bleomycin or saline. The results obtained were as follows: 1. Lung wet weight of treatment groups of rats was increased significantly while body weight gain of them was decreased significantly in comparison with that of control groups of rats (p<0.01). 2. The ratio(%) of lung wet weight to final body weight of treatment groups of rats was increased significantly in comparison with that of control groups of rats (p<0.01). 3. The main histopathological findings of lungs observed in rats at 7th day after dosing with bleomycin were proliferation of the type II alveolar epithelial cells and fibroblasts, increased invading of macrophages into lesions, round cell infiltration and perivascular edema. 4. Lung fibrous tissues were markedly increased in rats observed at 14th day after dosing with bleomycin. 5. Pumonary lesions observed in rats dosed with bleomycin and antioxidants(vitamin E, selenium, coenzyme $Q_{10}$) were not significantly different from those of rats given bleomycin alone.

Cyclophosphamide를 투영한 NOD 마우스의 신장에 대한 형태학적 연구

이준섭,성제경,Lee, Joon-sup,Seong, Je-kyung 대한수의학회 1994 大韓獸醫學會誌 Vol.34 No.4

This study was carried out to investigate diabetic renal changes in cyclophosphamide(CY) treated nonobese diabetic(NOD) mice and to develop animal model of diabetic human nephropathy. The 8-week-old NOD and ICR mice were injected with cyclophosphamide intraperitoneally at 200mg/kg body weight and compared the chemical effects on these mice with the non-treated NOD and ICR mice respectively. The renal glomeruli in ICR, cyclophosphamide-treated ICR(ICR+CY), NOD and cyclophosphamide-treated NOD(NOD+CY) mice were observed by the light and electron microscopes. The results obtained were summarized as follows ; 1. Spontaneous incidences of diabetes mellitus in NOD mice were significantly promoted by dosing with cyclophosphamide. 2. Glomerulohypertrophy, proliferation of mesangium, partial thickening of glomerular basement membrane, and partial fusion of pedicels of podocyte were observed in NOD mice and NOD+CY mice. These changes were not observed in both ICR mice and ICR+CY mice. 3. The diabetic nephropathy observed in NOD+CY mice was more severe than that of non-treated NOD mice.

한우(韓牛)의 섬유소성(纖維素性) 늑막염(肋膜炎)에 관(關)한 병리학적(病理學的) 검색(檢索)

이준섭,임창형,Lee, Joon Sup,Lim, Chang Hyeong 대한수의학회 1965 大韓獸醫學會誌 Vol.5 No.1

Throughout the studies the followings were obtained and summarized here. 1. Thickenings of plerua were due to the organized fibrin deposition on the pleural surface. 2. Thickenings of plerura were accompanied with increaseness of fibrous connective tissues in the interlobular and alveolar septa. 3. Eosinophilic inclusions seemed to LCL bodies were observed in the cytoplasms of epithelial cells of bronchi, bronchial glands and in the cytoplasms of mononuclear cells. 4. Histo-pathologically, there were some evidences influenced by viruses and the organisms of Psittacosis-Lymphogranuloma Venereum Group.

COVID-19 바이러스 확산 방지와 실내 공기 질 개선을 위한 스터디 카페 내 기계환기 실증 연구

이준섭(Joon Sup Lee),이예호(Ye Ho Lee),박상태(Sang Tae Park) 대한설비공학회 2021 대한설비공학회 학술발표대회논문집 Vol.2021 No.6

In this study, we consider the effect of improving indoor air quality due to the implementation of mechanical ventilation in two study cafes. The concentration of CO₂ was observed to assess the possibility of indoor spit particles spread infection due to the respiration. If mechanical ventilation equipment is not operated at both sites, the concentration of CO₂ tends to rise continuously as the concentration and real time of the inmates increases. On the other hand, operating mechanical ventilation equipment showed a reduction in CO₂ concentration. Reduction effects of indoor air pollutants PM<SUB>2.5</SUB>, TVOCs, and HCHO were considered. In both sites, the daily average concentration of all pollutant items, including PM<SUB>2.5</SUB>, decreased under operating conditions compared to the non-ventilating conditions of the machine. In order to prevent the spread of COVID-19 and improve indoor air quality, ventilation must be carried out, but it is recommended to use mechanical ventilation equipment with HEPA filters to prevent the inflow of fine dust from outside.