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In Situ Bacterial Detection Based on Carbon Dot-Dopamine Interactions
이주훈,정현정 한국공업화학회 2018 한국공업화학회 연구논문 초록집 Vol.2018 No.0
For the early detection of bacterial infection in several conditions, demands on fast and cheap detection methods of bacteria in biomedical science are increasing. However, while current bacterial detection methods such as microbial culture, and molecular diagnosis are sensitive enough, show limitations in time and simplicity. In this study, we first introduce a concept of a simple and fast assay using carbon dot nanoparticles with fluorescence, which can be switched on and off depending on the presence of bacteria as the target. Conducting the assay just requires the mixing of the carbon dot solution with dopamine along with the sample including bacteria. Fluorescence signal turned off with polydopamine formation while the existence of bacteria caused signal to turn on. With further research, this method can be applied as the simple and fast diagnosis of bacteria, without using expensive methods for bacterial detection.
Tumor-targeted delivery of CRISPR plasmid using HA-functionalized carbon dots
이주훈,정현정 한국공업화학회 2019 한국공업화학회 연구논문 초록집 Vol.2019 No.0
Gene therapy is a promising technology for cancer treatment due to the versatility and specificity of the drug. The CRISPR/Cas9 system has been attractive as a gene therapy since the target gene can be reprogrammed with high efficiency and selectivity. A non-viral method can effectively deliver the cargo to the target site without the problem from the viral method. Herein, polyethyleneimine carbon dots (PEI Cdots) were used to package efficiently the CRISPR plasmid and delivery them into cancer cells. A CRISPR plasmid which expressing Cas9 and sgRNA was prepared, complexed with PEI Cdots and functionalized with hyaluronic acid (HA) to both stabilize the complex and specifically target CD44 expressed on tumor cells. The HA-functionalized PEI Cdot/plasmid complex was characterized by dynamic light scattering and zeta potential measurements. Delivery of the complex into tumor cells was investigated and observing cellular uptake and knockout of the reporter gene by confocal microscopy.
Facile method for immobilization of genome editing protein based on dopamine polymerization
이주훈,정현정 한국공업화학회 2019 한국공업화학회 연구논문 초록집 Vol.2019 No.0
The CRISPR/Cas9 has attracted great attention due to the high simplicity and selectivity in editing the gene of interest. Immobilization of the CRISPR system on nonfunctionalized substrate surfaces with high efficiency would have various applications in biomedical research and diagnostic purposes. In this study, we introduce a facile method for surface immobilization of the CRISPR ribonucleoprotein mediated by polydopamine formation. A polypropylene surface was modified by dopamine polymerization, which can then readily react with the Cas9 protein by nucleophilic addition reactions. The efficiency of immobilization and bioactivity of Cas9 was characterized. The current approach can be used for immobilization of various proteins onto surfaces that are difficult to chemically functionalize while maintaining protein bioactivity.
사회적 단서로 행동적 동질성 획득과 긍정적 정서가 확산적 사고에 미치는 영향
이주훈,성민선 예술과미디어학회 2012 예술과 미디어 Vol.11 No.1
In this Study, we argue that being mimicked by an interaction partner cues will increase divergent thinking rather than not being mimicked and being induced by positive emotion facilitate divergent thinking rather than being induced by negative emotion regardless of being mimicked or not. To test the hypotheses, we experimentally manipulated whether divided 154 students who major in design being mimicked into or not, and performed Word Association Test and Remote Associate Test by inducing positive emotion and negative emotion to analyze it. The result that in support of hypothesis being mimicked leads people to instigate divergent thinking. But the participants who were induced by negative emotion created a higher proportion of divergent thinking evaluations than those who were induced by positive emotion. There was no interaction between variable and condition. 본 연구는 사회적 단서로서 행동적 동질성 획득의 영향을 받으면 그렇지 않은 집단 보다 확산적 사고를 더 할 것이며, 행동적 동질성 획득 유무에 상관없이 긍정적 정서가 유발되면 부정적 정서가 유발되는 상태에서보다 확산적 사고를 더 할 것이라는 두 가지 가설을 검증하는 것이다. 이를 위해 디자인전공 학생들 154명을 대상으로 행동적 동질성 유발 그룹과 그렇지 않은 그룹으로 나누어 흉내 내기 및 흉내 냄 당하기 그리고 긍정 및 부정적 정서를 조작한 후 단어연합과제와 원격연상검사를 실시하여 이들 두 그룹과 두 조건간의 확산적사고 능력을 비교분석하였다. 그 결과 행동적 동질성이 획득되면 확산적 사고를 더 가능하게 한다는 첫 번째 가설을 지지했으나 부정적 정서 유발 그룹이 긍정적 정서 유발 그룹보다 더 확산적 사고 평가가 높게 나와 두 번째 가설을 지지하지 못하였다. 그리고 변인과 조건간의 상호작용도 전혀 없었다.
이주훈,윤용범,차승태,이진,최종웅 대한전기학회 2003 전기학회논문지A Vol.52 No.7
- This paper discusses how to implement and verify a software model of the digital relay that can be added to real time digital simulator(RTDS) model library and is then subjected to the same outputs as the actual relay. The software model is stand-alone and can be used with real relays. It is also possible to conduct interactive real-time tests when the system effects of the relay action need to be investigated. The characteristics of mho type and the quadrilateral type, which is commonly used in recently developed relays, are modeled in this paper. Single circuit line and double circuit line system are used for model verification. The transmission lines are each 100 km in length and are modeled as distributed parameter lines but not frequency dependent. The transmission lines in the single circuit system are modeled as ideally transposed line. The mutual coupling data with the parallel line was taken account in the transmission lines for the double circuit system. The main CTs and PTs are included and operated in their linear region during the tests. For the purpose of testing the relay model accuracy the faults have been applied at various points on the protected line. Its accuracy is assessed against theoretical values.
1P-255 Fluorescence on-off nanosensing of protein using carbon dots and dopamine
이주훈,정현정 한국공업화학회 2017 한국공업화학회 연구논문 초록집 Vol.2017 No.1
For the early detection of severe disease in every world, demands on fast and cheap detection methods of protein disease markers in biomedical science are increasing greatly. However, while current protein detection methods such as HPLC, and Western blotting are sensitive enough, show limitations in point-of-care testing. Several studies using fluorescence nanoparticles to overcome this limitation are ongoing, but still need more time. In this research, we introduce a simple and fast assay using carbon dots with fluorescence which can be switched on and off depending on the presence of the protein target. The assay just required the mixing of the carbon dot solution with dopamine along with the solution including the target. Fluorescence signal turned off with polydopamine formation while the addition of a target protein caused signal to turn on. With further research, method can be applied as a simple and rapid diagnostic assay for ubiquitous detection of various protein targets.