http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
몇종의 세균과 Saccharomyces cerevisiae에 대한 식품첨가물의 향균 특성
이종수,오준세,김나미,금종화,이석건,Lee, Jong-Soo,Oh, Jun-Sei,Kim, Na-Mi,Keum, Jong-Hwa,Lee, Suk-Kun 배재대학교 자연과학연구소 1996 自然科學論文集 Vol.8 No.2
현재 식품첨가물로 많이 사용되고 있는 유기산과 안정제 및 색소 등의 각종 세균과 Sacch. cerevisiae 에 대한 향균 특성을 조사하였다. 산도 조절용으로 사용되고 있는 젖산, 사과산, 호박산 및 주석산은 L. acidophilus와 Sacch. cerevisiae에 대하여 향균성이 없었으나 B. subtilis등의 세균에 대하여는 향균성이 있었고 특히 사과산은 P. aeruginosa에 대하여 강한 향균력이 있었다.(최소생육저지농도 : 0.05%). 안정제로서의 알긴산과 펙틴은 B. subtilis, E. coli, P. aeruginosa에 대하여 비교적 강한 향균성을 보였고 L. acidophilus에 대하여는 향균성이 없었다. 황색 색소(홍화엘로우)와 적색 색소(Red powder-N)는 향균성이 없었고 표백제인 $NaHSO_3$의 세균에 대한 최소생육저지농도는 0.05%, Sacch. cerevisiae에 대하여는 0.5%로 향균성이 있었다. In order to survey the safety of some food additives, antimicrobial activity of acidulants, stabilizers, antioxidants, natural coloring materials and bleaching agents against 5 strains of bacteria and Sacch. cerevisiae were investigated by dilution method and minimal inhibitory concentration(MIC) method. Malic acid as acidulants displayed the effective antimicrobial activity in vitro against P. aeruginosa and its MIC is 0.05%. Alginic acid and pectin as stabilizer also displayed strong antimicrobial activity against B. subtilis, E. coli and P. aeruginosa, and tannin(antioxidants) and $NaHSO_3$ displayed antimicrobial activity against all bacteria tested. However gums(Arabia, Xanthan, Gua) and natural coloring materials(Hongwha Yellow, Red powder-N) were not affected to growth of bacteria and Sacch. cerevisiae.
정상 및 원추각막에서 각막 전, 후면의 융기 및 시상면만곡도의 비교분석
이종수,이승욱,이승민,이미현,Jong Soo Lee,Seung Wook Lee,Seung Min Lee,Mi Hyun Lee 대한안과학회 2012 대한안과학회지 Vol.53 No.10
Purpose: To compare changes of anterior and posterior corneal elevation, and sagittal curvature according to the severity of keratoconus and to compare differences between keratoconus and normal cornea. Methods: A total of 81 eyes diagnosed with keratoconus and 20 eyes of normal subjects were evaluated with a Pentacam Scheimpflug camera. The keratoconus eyes were divided into 3 groups according to mean keratometer (K): mild (K ≤ 47.0 diopters (D)), moderate (47.0 to 52.0 D), and severe (52.0 D≥). The following parameters were obtained to evaluate the correlation of keratoconus: corneal thickness, anterior and posterior corneal elevation, and sagittal curvature. Results: Out of 81 keratoconus eyes, 56 eyes were mild, 12 eyes were moderate, and 13 eyes were severe keratoconus. The mean central corneal keratometer, anterior and posterior corneal elevation, and sagittal curvature of the keratoconus eyes were 49.7 D, 22.07 μm, 38.16 μm, 52.76 D and the values increased statistically compared to the normal eyes. Furthermore, the values increased significantly with the severity of keratoconus. ROC curve analysis showed the estimated meaningful value for anterior and posterior corneal elevation and sagittal curvature of keratoconus for diagnosis; there were no diagnostic values for corneal thickness and refractive power. Conclusions: The index of 5.5 μm for mean anterior elevation, 12.5 μm for mean posterior elevation, and 44.5 μm for mean sagittal curvature using the PentacamⓇ are useful to diagnose keratoconus. Variation of anterior and posterior elevation, and sagittal curvature measured by PentacamⓇ are useful in understanding the process of keratoconus. J Korean Ophthalmol Soc 2012;53(10):1432-1437
이종수,이승욱,이상준,김나미,Jong Soo Lee,Seung Wook Lee,Sang Jun Lee,Na Mi Kim 대한안과학회 2012 대한안과학회지 Vol.53 No.3
Purpose: To evaluate the response and cellular damage of cultured human pterygial cells according to the concentration and exposure time of topical cyclosporin. Methods: Human pterygial cells were exposed to a cyclosporin A concentrations of 0.1 μg/ml (0.0001%), 1 μg/ml (0.0001%), 10 μg/ml (0.001%), 100 μg/ml (0.01%), or 500 μg/ml (0.05%) for 5 or 10 minutes. An MTT-based colorimetric assay was performed to assess the metabolic activity of cellular proliferation, and a lactate dehydrogenase (LDH) leakage assay was used to determine cellular damage. The extra-cellular matrix of PIP, laminin and MMP were evaluated, and the measurement of pro-inflammatory cytokine, TNF-α and IL-1b. IL-6, IL-8 was performed using ELISA kits. Results: The pterygial cellular inhibitory effect of cyclosporin was similar to that of the control according to the concentration and exposure time (p > 0.05). Compared with the control, the level of LDH did not show a statistically significant difference between concentration and exposure time (p > 0.05). There was no significant difference of inhibitory effects by PIP, laminin, or MMP between the experimental and control groups (p > 0.05). The production of TNF-α and IL from the experimental pterygial cells due to the effect of cyclosporin was not significantly different from that of the control at a longer exposure time or stronger concentration (p > 0.05). Conclusions: The response of pterygial cells to topical cyclosporin A at concentrations less than 0.05% for less than 10 minutes of exposure time showed no prevention of pterygial recurrence. With regard to cellular damage, little effects on inhibition of PIP, laminin, MMP, IL, and TNF-α were observed compared with that of the control. J Korean Ophthalmol Soc 2012;53(3):466-472
겔형태 인공누액이 각막실질세포 및 결막상피세포에 미치는 영향
이종수,김은희,김수진,이지은,김나미,Jong Soo Lee,Eun Hee Kim,Soo Jin Kim,Ji Eun Lee,Na Mi Kim 대한안과학회 2010 대한안과학회지 Vol.51 No.12
Purpose: To evaluate the biological effects and cytotoxicity of gel-type artificial tears on human corneal keratocytes and conjunctival cells in vitro. Methods: Human corneal keratocytes and conjunctival epithelial cells were exposed to Soothe® and Systane® at variable concentrations. Evaluations were conducted through an MTT-based calorimetric assay to measure the metabolic activity and through a lactate dehydrogenase (LDH) assay to assess cellular damage. Apoptotic response was examined using fluorescent microscopy and flow cytometric analysis, and cellular morphologic results were evaluated with a transmission electron microscope. Results: The inhibitory effects of corneal keratocyte and conjunctival cell proliferations increased at higher concentrations and longer exposure times to Soothe® and Systane®. The LDH titers increased after Soothe® exposure, but showed no significant difference after Systane® exposure. Soothe® and Systane® treatments both produced fluorescence, representing apoptotic cells. In flow cytometry, the maximal apoptotic response was observed for both types of artificial tears, although Systane® showed less edema, as well as reduced cytoplasmic and nuclear cell degeneration compared to those of Soothe®. Conclusions: The apoptotic responses of Soothe® and Systane® are associated with inhibitory effects of human corneal keratocyte and conjunctival epithelial cell proliferations. To inhibit the cellular proliferation of human corneal keratocytes and conjunctival epithelial cells, Systane® may be less severe than Soothe® at higher concentrations and longer exposure times. J Korean Ophthalmol Soc 2010;51(12):1643-1651
이종수,Lee, Jong-Soo 한국정보처리학회 2003 정보처리학회논문지B Vol.10 No.2
본 논문에서는 실사적인 3D 물체 모델링을 위해 제안된 기법들을 고찰하고, 보다 정확한 모델링을 위한 방안들을 제시하고 있다. F-행렬 추정기법의 개선과 스테레오 영상 평행화기법을 적용함으로 물체 모델링의 정확도를 높히는데 필수적임을 보여준다. In this paper, in-depth considerations are given to various techniques suggested for photographic 3D modelling and possible enhancements of the techniques are discussed. It is found that both the improvement of fundamental matrix estimation techniques and the stereo image rectification processing stage are necessary for more accurate 3D modelling.
이종수,김은희,김수진,Jong Soo Lee,MD,PhD,Eun Hee Kim,MD,Su Jin Kim,MD 대한안과학회 2011 대한안과학회지 Vol.52 No.7
Purpose: To investigate the biologic effects of topical calf serum on corneal epithelial cells in vitro. Methods: The effects of calf serum on the corneal epithelial cells were evaluated using the MTT (3-[4,5-Dimethylthiazol- 2-yl]-2,5-diphenyltetrazolium bromide) assay, and the concentration of IL-1α, TGF-β1 and MMP-9 in the cells was measured. Cell damage was determined using lactate dehydrogenase (LDH), and cellular morphologies were examined by transmission electromicroscopy. Results: Metabolic activity of the corneal epithelial cells decreased at higher concentrations and longer exposure durations. IL-1α, TGF-β1 and MMP-9 titers were lower in calf serum-treated cells than in the control. LDH and cellular damage to the corneal epithelial cells, such as chromatin margination and cytoplasmic organelle swelling, were prominent in cells treated with 30% calf serum. Conclusions: Cellular metabolic activity was higher and cellular toxicity was lower in cells treated with 10% calf serum compared to those treated with the 20% and 30% concentrations. Furthermore, inflammatory cytokines were sufficiently inhibited in cells treated with the 10% solution. These results indicate that 10% calf serum could be used clinically. J Korean Ophthalmol Soc 2011;52(7):852-858
Candida sp. L-16이 생성하는 Xylitol dehydrogenase의 생성조건에 관한 연구
이종수 대구산업정보대학 1995 논문집 Vol.9 No.2
The opitmal cultural conditions such as carbon and nitrogen sources, cultural temperature, initial pH, aeration and cultural time for production xylitol dehydrogenase which catalyzed the conversion of xylitol to xylulose from Candida sp. L-16. were investigated. The maximum yield of xylitol dehydrogenase was observed with cultivation in a medium containing 5.0% D-xylose as a carbon source and 0.3% yeast extract, 0.3% malt extract and 0.5% bactopeptone as nitrogen sources and pH 4.5. The optimal conditions for production of xylitol dehydrogenase were 28℃ of cultural temperature and 4.5 of initial pH. It was found that the production of enzyme reached a maximum in a 3 days of cultivation in the 100㎖Erlenmeyer flask containing 40㎖ broth.
피기백 인공수정체를 이용한 백내장 수술 후의 잔존 굴절 이상의 교정 1예
이종수,김영희,박정열,김성일,Jong Soo Lee,MD,PhD,Young Hee Kim,MD,Jung Yul Park,MD,Sung Il Kim,MD 대한안과학회 2014 대한안과학회지 Vol.55 No.12
Purpose: To report a case of corrected residual refractive error after cataract surgery with sulcoflex piggyback intraouclar lens (IOL). Case summary: A 77-year-old man was diagnosed as having hypermature cataract in the right eye and his corrected visual acuity in the same eye was hand-motion before surgery. Refractive error was +9.0 diopter (D) 6 months after conventional cataract surgery in the right eye. The authors performed additional cataract surgery using a piggyback method inserting a +10.0 D IOL in the ciliary sulcus. Four weeks after surgery, his refractive error was -1.25 D, visual acuity increased to 120/200 and 60/200 (with and without correction, respectively). No complication was observed during follow-up time and the patient was satisfied with his results. Conclusions: Correction of residual refractive error after cataract surgery with sulcoflex piggyback IOL is safe and effective. J Korean Ophthalmol Soc 2014;55(12):1890-1894