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차광종(Gwang Jong Cha),송진욱(Jin Wook Song),조홍찬(Hong Chan Jo),김용휘(Yong Hwi Kim),유대열(Dae Yeol Yoo),이중복(Joong Bok Lee),이종기(Jong Gee Lee),유제현(Je Hyun Yoo),Taniguchi Koki 대한바이러스학회 1999 Journal of Bacteriology and Virology Vol.29 No.2
Rotaviruses belong to Reoviridae causes diarrhea in human beings as well as domestic animals. This study was conducted to see what type of human rotaviruses are distributed in Seoul and Kyung-gi province. Twenty two of 81 patients showed rotavirus positive with diagnostic kit and RNA electropherosis. We isolated all of rotaviruses from the patients. Electropherotypes of 22 isolates showed 4:2:3:2 pattern whereas those migration patterns were long type. All of those isolates belonged to group A. Twenty out of 22 isolates reacted with monoclonal antibodies specific to G1, P1A and subgroup II, whereas rest of them, A-29 and K-30 reacted with subgroup I specific monoclonal antibody. The nucleotide sequence of an isolate K-21 showed 98-100% and 90-96% homologies with those of Wa and KU strain, respectively.
락토페린이 국내분리 유아 로타바이러스의 MA 104세포 감염에 미치는 영향
차광종(Gwang Jong Cha),유대열(Dae Yeol Yoo),이종기(Jong Gee Lee),유제현(Je Hyun Yoo) 대한바이러스학회 1999 Journal of Bacteriology and Virology Vol.29 No.2
It has long been known that lactoferrin prevents human beings from infection of virus. To prove this activity of lactoferrin, we evaluated the activities of different lactoferrins to an isolate human rotavirus K-21. Bovine lactoferrin inhibited infection of K-21 to MA-104 cell at the concentration of 25.9 ㎛ whereas bovine hydrolysed lactoferrin prevented rotavirus infection at 103.8 ㎛. However human lactoferrin prevented infection of K-21 at the concentration of 217.5 ㎛. These data suggested that lactoferrin activity may be unaffected by the intestinal digestive enzymes and bovine lactoferrin is more active than human lactoferrin with respect to prevention of rotavirus infection.