자외선이 조사된 HM3KO세포의 멜라닌과 nitric oxide 생성에 대한 rutin의 효과

이승연(Seung Youn Lee),김영목(Yeong Mok Kim),이재규(Jai Kyoo Lee),유수진(Su Jin Yoo),문연자(Yeun Ja Mun),우원홍(Won Hong Woo) 대한해부학회 2003 Anatomy & Cell Biology Vol.36 No.1

자외선은 사람 피부에서 멜라닌 생성을 촉진시키는 가장 주요한 요인으로 알려져 있으며, 최근 ultraviolet B (UVB)에 의한 피부 색소침착에 nitric oxide (NO)가 관여하고 있다고 보고되었다. Rutin은 야채나 과일 등의 식물체에 널리 분포된 flavonoid로서, 항바이러스, 항산화효과가 보고되어 본 연구에서 UVB-유도 멜라닌 생성과 NO 생성에 대한 rutin의 효과를 조사하였다. UVB 조사에 의하여 HM3KO세포에서 멜라닌양과 멜라닌 생성에 관련된 tyrosinase 효소의 활성이 증가되었고, rutin은 UVB에 의해 증가된 멜라닌과 tyrosinase 활성을 농도 의존적으로 억제하였다. 이와 더불어 UVB 조사에 의하여 HM3KO세포에서 NO 생성이 증가되었으며, rutin은 농도 의존적으로 이를 억제하였고, UVB에 의해 감소된 세포증식을 회복시켰다. 또한 exogenous NO donor (sodium nitroprusside, SNP)에 의해서도 HM3KO세포의 멜라닌 생성이 증가되었으며, rutin은 효과적으로 이를 억제하였다. 이상의 결과 rutin은 UVB 조사에 의하여 촉진된 멜라닌 생성을 억제하였으며, 이는 rutin이 UVB에 의해 증가된 NO 생 성을 억제함으로서 부분적으로 멜라닌 생성조절에 관여할 것으로 생각된다. Ultraviolet (UV) radiation is the main physiological stimulus for human skin pigmentation. Recently, nitric oxide (NO) have been involved in mediation of skin pigmentation induced by UVB. Rutin, a flavonoid of vegetables and fruits, has antiviral and antioxidant properties. Therefore, we investigated the effect of rutin on UVB-induced melanogenesis and NO production in HM3KO cells. In this study, we demonstrated that UVB-irradiation stimulated melanin content and tyrosinase activity in HM3KO cells. Rutin suppressed UVB-stimulated total melanin content and tyrosinase activity in a dose-dependent manner. Additionally, we showed that UVB-irradiation stimulated NO production in HM3KO cells. Rutin also suppressed UVB-induced NO production and repaired reduction of cell proliferation by UVB. UVB stimulation of melanogenesis was mimicked by exogenous NO donor (sodium nitroprusside, SNP), and rutin effectively suppressed it. Therefore, we concluded that rutin suppressed UVB-stimulated melanogenesis and that it is involved in melanogenesis regulation partially through the suppression of UVB-induced NO production.

Nitrofen투여시 쥐에서 유발되는 선천성 횡격막 결손증: 동물모델

이환봉(Whan Bong Lee),이재규(Jai Kyoo Lee),이병찬(Pyong Chan Lee),소병준(Byung Jun So),채권묵(Kwon Mook Chae) 대한외과학회 2001 Annals of Surgical Treatment and Research Vol.60 No.4

과산화수소로 손상된 랫드(흰쥐) 배양 심근세포에 대한 천오두의 영향에 관한 연구

서은아 ( Eun A Seo ),이재규 ( Jai Kyoo Lee ),이종화 ( Joung Hwa Lee ),이병찬 ( Byung Chan Lee ),이강창 ( Kang Chang Lee ),서부일 ( Bu Il Seo ),송호준 ( Ho Joon Song ) 대한본초학회 2003 大韓本草學會誌 Vol.18 No.2

N/A Objectives : To examine the cytotoxicity and the effect of Aconiti Radix(AR) on oxygen free radicals in cultured myocardial cells of neonatal rat. Methods : Toxic effect was measured by MTT assay after myocardial cells were incubated for 4 hours in the media containing 10-40 PM concentrations of H202. In addition, the protective effect of AR was determined in these cultures. Results : Cell viability was positively decreased in a dose-dependent manner after exposure of 30 μM H₂O₂to cultured rat myocardial cells for 4 hours. In the protective effect, AR prevented the H₂O₂-induced cytotoxicity in these cultures. Conclusion : From these results, it suggests that H₂O₂has cytotoxicity in cultured rat myocardial cells and AR blocked the cytotoxicity induced by H₂O₂.

담배 니코틴에 의한 사람 태아 성상세포에서 종양괴사인자(TNF-α)의 발현 억제작용

손일홍,이성익,양현덕,한선정,석승한,이재규,김재현,박주영,문형인,이성수,Son, Il-Hong,Lee, Sung-Ik,Yang, Hyun-Duk,Han, Sun-Jung,Suk, Seung-Han,Lee, Jai-Kyoo,Kim, Jae-Hyun,Park, Joo-Young,Moon, Hyung-In,Lee, Sung-Soo 대한화학회 2007 대한화학회지 Vol.51 No.3

니코틴은 사람 대식세포에서 interleukin 2 (IL-2)와 종양괴사인자 (tumor necrosis factor-alpha; TNF-α) 가 생성되는 것을 억제하는데, 이러한 억제작용은 cytokine 유전자 발현 중 전사단계에서 전사인자의 활성을 억제함으로써 일어난다. 이러한 니코틴의 면역반응 억제작용은 아프타성궤양 및 궤양성대장염, 알레르기성폐 포염, 건초열 등에서도 보고되고 있다. 만일 중추신경계에서도 위와 같은 니코틴의 면역억제 작용이 일어난 다면 다발성경화증과 같은 면역반응 매개질환의 치료에 새로운 전기가 마련될 수 있을 것이다. 본 연구에서 는 중추신경계의 여러 면역반응 매개질환의 병태생리에 대한 이해를 넓히고자, 이미 알려진 니코틴의 cytokine 생성억제가 사람 중추신경계의 성상세포에서도 일어남을 확인하고 그 억제기전을 밝히고자 하였다. 이를 위 하여 사람 태아 성상세포에 다양한 농도의 니코틴과 IL-1β를 처리한 다음 TNF-α mRNA의 발현 정도와 NF- κB의 활성을 비교, 분석하여 다음과 같은 결과를 얻었다. 1. 사람 태아 성상세포를 0.1-20 μg/ml의 니코틴으로 처리해 본 결과 10 μg/ml 이상의 농도에서 세포독성능이 나타나기 시작하였다. 2. 사람 태아 성상세포에 IL- 1β를 처리하면 2시간만에 TNF-α mRNA가 최대로 발현되었으며 그 이후로는 점진적으로 감소하였다. 3. 사 람 태아 성상세포를 1 및 0.1 μg/ml의 니코틴으로 전처리한 후 IL-1β로 자극한 군에서는 IL-1β 단독 처리군에 비해 TNF-α mRNA의 발현이 감소하는 양상을 보였다. 1 μg/ml의 니코틴을 처리한 경우에는 8시간 이후부터 TNF-α mRNA의 발현이 현저하게 감소하여 12시간에 최대로 감소하였다. 또한 0.1 μg/ml의 니코틴을 처리한 군에서는 24시간에 가장 현저하게 감소하였다. 4. 성상세포에 IL-1β로 처리한 군에서는 강력한 NF-κB의 활성 을 확인할 수 있었으며, 니코틴을 전처리하고 IL-1β 자극한 군에서는 NF-B의 활성이 감소하였다. 결론적으로 일정농도 이상의 니코틴은 세포독성효과를 나타내나 적정한 농도와 시간 경과후 니코틴은 사람 태아 성상세포에서 IL-1β에 의해 유도되는 TNF-α의 발현 감소를 유도하며, 이는 NF-κB의 활성을 감소시킴으로써 나타난다고 생각된다. The Tumor necrosis factor-α, (TNF-α), is involved in the pathogenesis of multiple sclerosis and contributes to the degeneration of oligodendrocytes as well as neurons. Nicotine has been found to have immunosuppressive and inflammation-suppressing effects. Astrocytes, the major glial cells in the CNS, are capable of producing TNF-α at both the mRNA and protein levels in response to interleukin-1 (IL-1) or TNF-α. Nicotine has been shown to influence glial cell functions. To order to explore the role of astrocytes in the production of TNF-α, astrocytes were pretreated with nicotine and are stimulated with IL-1β to determine their effects on TNF-α production. The results are as follows. Cytotoxic effects of nicotine on human fetal astrocytes were noted above 10 μg/ml of nicotine. The effect of IL-1β on TNF-α mRNA expression in primary cultured human fetal astrocytes was maximal at 2 h after IL- 1β(100 pg/ml) treatment. Human fetal astrocytes were pretreated with 0.1, 1, and 10 μg/ml of nicotine and then stimulated with IL-1β (100 pg/ml) for 2 h. The inhibitory effect of nicotine on expressions of TNF-α mRNA in human fetal astrocytes with pretreated 0.1 μg/ml of nicotine is first noted at 8 hr, and the inhibitory effect is maximal at 12 h. The inhibitory effect at 1 μg/ml of nicotine is inhibited maximal at 24 h. Nicotine at 0.1, 1 and 10 μg/ml concentrations significantly inhibits IL-1β-induced NF-κB activation. Collectively, this study indicates that nicotine might inhibit the expression of TNF-α in activated human fetal astrocytes.

동과자가 Glucose Oxidase로 손상된 혈관내피세포에 미치는 영향

서은아 ( Eun A Seo ),최승석 ( Natthew Seung Suk Choi ),이병찬 ( Byung Chan Lee ),이재규 ( Jai Kyoo Lee ),이강창 ( Kang Chang Lee ),정종길 ( Jong Gil Jeong ),신민교 ( Min Kyu Shin ),송호준 ( Ho Joon Song ) 대한본초학회 2003 大韓本草學會誌 Vol.18 No.2

N/A Objectives : It has been demonstrated that oxidative stress of oxygen radicals is involved in vascular disorder. The aim of this study was to evaluate the cytotoxicity of glucose oxidase (GO) and protective effect of herb extract on GO-induced cytotoxicity in the cultured vascular endotherial cell (VEC) of mouse. Methods : Cytotoxic effect of GO and protective effect of Benincasae Semen (BS) were performed by MTT assay, after cultured VEC were incubated in the media containing 1-30 mU/ml GO for 6 hours. Results : GO decreased cell viability in dose-and time-dependent mannner, and BS increased cell viability decreased by GO-induced cytotoxicity in these cultures. Conclusion : From above the results, GO has cytotoxic effect, and herb extract, BS is very effective in blocking GO-induced cytotoxicity in cultured VEC.

釣鈞藤이 해마신경세포의 산화적 손상에 미치는 효과

이재규,최유선,이은미,장철호,이강창,박승택,이형철,황상구,주성민,김원신,백은경,전병훈 대한동의병리학회 2001 동의생리병리학회지 Vol.15 No.5

To clarify the neurotoxicity of hydrogen peroxide(H2O2) in cultured hippocampal neurons derived from neonatal mouse, cytotoxicity was measured by MTS assay after cultured cells were grown for 5 hours in the media containing 1∼20μM concentrations of H2O2. In addition, the neuroprotective effect of Uncariae Ramulus Cum Uncis was measured in these cultrures. Cell viability was positively decreased in a dose-dependent manner after exposure of cultured mouse hippocampal neurons to 10μM H2O2 for 5 hours. In the neuroprotective effect of Uncariae Ramulus Cum Uncis on H2O2-mediated toxicity, Uncariae Ramulus Cum Uncis prevented the H2O2-induced neurotoxicity in these cultures. From these results, it suggests that H2O2 is toxic in cultured hippocampal neurons derived from neonatal mouse and selective herb extract such as Uncariae Ramulus Cum Uncis is effective in prevetion of the neurotoxicity induced by H2O2.

카드뮴에 의한 희돌기교세포의 손상에 대한 牛膝의 영향

이재규,이은미,오석규,손영우,유교상,김상수,이정헌,이강창,한두석,이승현,박승택,김요한,송호준 대한동의생리학회,대한동의병리학회 2001 동의생리병리학회지 Vol.15 No.6

In order to clarify the cytotoxic effect of cadmium in cultured oligodendrocytes of neonatal mouse, the cytotoxicity was measured by MTX assay in cultured cells treated with 1∼20uM CdCl_2 for 24 hours. And also, the protective effect of Achyranthis Bidentazae Radix(ABR) was examined by cell viability in these cultures. Cell viability was significantly decreased in a dose-and time-dependent manner after exposure of cultured oligodendrocytes to 10uM CdCl_2 for 24 hours. Protective effect of ABR on CdCl_2-mediated toxicity was very effective in these cultures. From above the results, it suggests that CdCl_2 is toxic in cultured oligodendrocytes and selective herb extract such as ABR is effective in protection of the CdCl_2 -induced cytoxicity.

반대측 선조체에 점삼 출현이 동반된 비케톤성 고혈당성 무도증

이재규,손일홍 圓光大學校 醫科學硏究所 2008 圓光醫科學 Vol.23 No.2

Hemichorea is usually caused by lesions in the contralateral subthalamus and/or basal ganglia. Brain MRI shows a high signal intensity on T1-weighted images and brain CT shows a hyperdense lesion in the contralateral lentiform nucleus in patients with nonketotic hyperglycemic chorea. It is not unclear that these pathophysiological mechanisms is ischemia or petechial hemorrhage. A 78 year-old woman with uncontrolled diabetic mellitus presented with sudden involuntary movement in the right limbs. Brain CT on the first HD(hospital day) revealed hyperdensity in the left lentiform nucleus and Brain MRI on the 2nd HD showed high signal intensity on Tl-weighted images in the left lentiform nucleus. After 2 month later, involuntrary movment was subsided, and the density was more decreased on follow-up brain CT. We suggest a possibility that nonketotic hyperglycemic hemichorea is associated with petechial hemorrhage in the contralateral lentiform nucleus. 편측무도증은 대부분 반대측 선조체의 문제로 발생된다. 비케톤성 고혈당 편측 무도증을 가지고 있는 환자의 뇌 단층촬영(Brain CT)에서 반대측 선조체의 고음영이 관찰되었고 뇌 자기공명영상(Brain MRI)에서는 T1 강조영상에서 고신호로 강조되었다. 하지만 고혈당과 관련된 편측 무도증의 정확한 기전은 아직도 명확하지 않으며 점상출혈이나 허혈성 변화일 것으로 추정되고 있다. 당뇨가 잘 조절되지 않는 78세 여자환자가 내원 20일전부터 발생한 우측 팔, 다리의 불수의적인 움직임을 주소로 입원하였다. 내원 초기 brain CT에서는 좌측 선조체의 고음영이 관찰되었고 Brain MRI에서는 T1 강조영상에서 고신호를 보였다. 증상 호전 보이면서 추적 관찰한 Brain MRI에서 이 병소는 T1 강조영상에서 고신호 강도가 더욱 증가하였으며, gradient echo에서는 저신호 강도가 더욱 증가하였다. 이후 증상이 더욱 호전되었고, Brian CT에서는 반대측 선조체의 고밀도 소견이 감소한 양상 관찰되어 점상 출혈 기전과 관련된 증례로 추정하여 보고하는 바이다.

중대뇌동맥 뇌경색 환자에서 고위험군에 관한 비교분석

이재규 대한응급의학회 1999 대한응급의학회지 Vol.10 No.4

To select high risk group in middle cerebral artery(MCA) infarction by comparing and analysing 10 risk factors in above patients, we analyzed 32 MCA infarcted patients and 30 case control groups retrospectively. Through the analysis of 10 risk factors(age, heart disease, blood pressure, total cholesterol, triglyceride, HDL-cholesterol, glucose, smoking, r-GTP, past history : hypertension or cerebrovascular accident) in MCA infarction by the times patients arrieves in emergency room by scoring system made by author, MCA infarcted patients showed score about 1.4 in 24 cases(75.00%) among 32 MCA infarction patients, and in control group, score below 1.4 showed in 28 cases(93.33%) among 30 cases By the scoring system made by author, the higher risk grouped patients could identified more easily in emergency department.

급성 약물중독에 관한 임상적 고찰

이재규,박재황 圓光大學校 醫科學硏究所 1993 圓光醫科學 Vol.9 No.1-2

Clinical observation was made on 405 cases of drug intoxication visited to WonKwang university hospital via emergency room during one year from Jnauary 1991 to December 1991. The results were summarized as follows ; 1. Annual incidence was 1.91% (405 of the 21247 patients). 2. Factors related to increased occurrence were male(53.83%), age of 3rd decade(26.67%), monday (17.78%), and August(17.28%). 3. The motives of most intoxication(88.89%) were suicidal attempts. 4. The most common drug of intoxication was insecticides(36.54%), the remainders were S.T.H.(17.04%), herbicides(11.36%), puffer(5.43%), rodenticides(4.49%), etc. 5. The chief complaints on arrival to emergency room were nausea & vomiting(36.79%), mental change(15.56%), dizziness(13.33%), general weakness(4.49%), etc. 6. The abnormal vital signs on arrival to emergency room were tachycardia(18.52%), mental change (15.56%) hypertension(9.63%), etc. 7. The observed abnormal laboratory findings on admission(27 cases) were elevated ALP(15 cases), leukocytosis(12 cases), depressed T.Protein, depressed HCO_3, hypocarbia etc, in order of frequency. 8. The complications of acute D-I were aspiration pneumonia(2.22%), G-I symtpom(1.23% ; ileus, peptic ulcer, gastritis etc.) parkinsonism(0.74%), etc. 9. The outcomes of the patients were recovery(63.46%), against discharge(26.42%), hopeless discharge(8.15%), death(1.98%)