Activation Mechanism of Arachidonic Acid in Human Neutrophil Function

심재건,이정수,신용규,이광수,Sim, Jae-Kun,Lee, Chung-Soo,Shin, Yong-Kyoo,Lee, Kwang-Soo The Korean Society of Pharmacology 1992 대한약리학잡지 Vol.28 No.1

칼슘을 함유하는 반응액에서 arachidonic acid는 뚜렷하게 superoxide와 $H_2O_2$의 생성을 자극하였고 NADPH oxidase를 활성화하였다. 칼슘이 없는 반응액에서 NADPH oxidase에 대한 arachidonic acid의 자극 작용은 나타나지 않았다. Arachidonic acid에 의하여 자극된 respiratory burst는 EGTA, TMB-8, verapamil, diltiazem, nifedipine, dibucaine, lidocaine, CCCP, 2,4-dinitrophenol, sodium arsenate, chlorpromazine, theophylline, $HgCl_2$, PCMB와 PCMBSA에 의하여 억제되었으나, tetrodotoxin, tetraethyl ammonium chloride와 procaine의 영향은 받지 않았다. EGTA는 거의 완전히 arachidonic acid에 의한 ${\beta}-glucuronidase$의 유리를 억제하였으며, verapamil, CCCP와 theophylline은 약간 억제하였으나, 이에 반하여 dibucaine은 유의한 효과를 나타내지 않았다. Arachidonic acid는 중성호성 백혈구로 부터 칼슘을 유리시켰으며, 이는 TMB-8에 의하여 감소되었다. Arachidonic acid에 의한 세포내 유리 칼슘 농도의 상승은 EGTA와 CCCP에 의하여 억제되었고 TMB-8에 의하여 약간 억제되었다. Arachidonic acid와 verapamil 또는 arachidonic acid와 dibucaine의 동시 첨가에 의하여 상승된 세포내 유리 칼슘 농도의 양은 arachidonic acid 단독에 의한 것보다 현저하였다. 이상의 결과로 부터 다양한 생화학적 변화가 arachidonic acid에 의하여 활성화된 중성호성 백혈구의 기능 표현에 관여할 것으로 시사된다. Arachidonic acid는 세포내 칼슘 저장고로부터 칼슘유리를 자극하여 세포내 유리 칼슘 농도를 상승시킬 것으로 추정된다. 중성호성 백혈구의 활성화증에 칼슘 유입과 유출은 동시에 이루어질 것으로 추정된다. In $Ca^{++}$ containing media, arachidonic acid markedly stimulated superoxide and $H_2O_2$ generation and activated NADPH oxidase. In $Ca^{++}$ free media, stimulatory action of arachidonic acid on NADPH oxidase was not detected. Arachidonic acid-stimulated respiratory burst was inhibited by EGTA, TMB-8, verapamil, diltiazem, nifedipine, dibucaine, lidocaine, CCCP, 2,4-dinitrophenol, sodium arsenate, chlorpromazine, theophylline, $HgCl_2$, PCMB and PCMBSA but not affected by tetrodotoxin, tetraethylammonium chloride and procaine. EGTA almost completely inhibited release of ${\beta}-glucuronidase$ by arachidonic acid and verapamil, CCCP and theophylline slightly inhibited it, whereas dibucaine did not show any significant effect. Arachidonic acid induced $Ca^{++}$ release from intact neutrophils and it was decreased by TMB-8. Arachidonic acid-induced elevation of intracellular free $Ca^{++}$ level was inhibited by EGTA and CCCP and slightly inhibited by TMB-8. Amount of intracellular free $Ca^{++}$ increased by either arachidonic acid plus verapamil or arachidonic acid plus dibucaine was greater than that by arachidonic acid alone. These results suggest that various changes of biochemical events may be implicated in the functional expression in neutrophils activated by arachidonic acid. Arachidonic acid appears to elevate cytosolic free $Ca^{++}$ level by stimulating $Ca^{++}$ release from intracellular $Ca^{++}$ storage sites. During activation of neutrophils, $Ca^{++}$ influx and efflux may be accomplished, simultaneously.

사람 중성호성 백혈구의 기능에 있어서 Arachidonic Acid의 활성화 기전

심재건(Jae-Kun Sim),이정수(Chung-Soo Lee),신용규(Yong-Kyoo Shin),이광수(Kwang-Soo Lee) 대한약리학회 1992 대한약리학잡지 Vol.28 No.1

칼슘을 함유하는 반응액에서 arachidonic acid는 뚜렷하게 superoxide와 H₂O₂의 생성을 자극하였고 NADPH oxidase를 활성화하였다. 칼슘이 없는 반응액에서 NADPH oxidase에 대한 arachidonic acid의 자극 작용은 나타나지 않았다. Arachidonic acid에 의하여 자극된 respiratory burst는 EGTA, TMB-8, verapamil, diltiazem, nifedipine, dibucaine, lidocaine, CCCP, 2,4-dinitrophenol, sodium arsenate, chlorpromazine, theophylline, HgCl₂, PCMB와 PCMBSA에 의하여 억제되었으나, tetrodotoxin, tetraethyl ammonium chloride와 procaine의 영향은 받지 않았다. EGTA는 거의 완전히 arachidonic acid에 의한 β-glucuronidase의 유리를 억제하였으며, verapamil, CCCP와 theophylline은 약간 억제하였으나, 이에 반하여 dibucaine은 유의한 효과를 나타내지 않았다. Arachidonic acid는 중성호성 백혈구로 부터 칼슘을 유리시켰으며, 이는 TMB-8에 의하여 감소되었다. Arachidonic acid에 의한 세포내 유리 칼슘 농도의 상승은 EGTA와 CCCP에 의하여 억제되었고 TMB-8에 의하여 약간 억제되었다. Arachidonic acid와 verapamil 또는 arachidonic acid와 dibucaine의 동시 첨가에 의하여 상승된 세포내 유리 칼슘 농도의 양은 arachidonic acid 단독에 의한 것보다 현저하였다. 이상의 결과로 부터 다양한 생화학적 변화가 arachidonic acid에 의하여 활성화된 중성호성 백혈구의 기능 표현에 관여할 것으로 시사된다. Arachidonic acid는 세포내 칼슘 저장고로부터 칼슘유리를 자극하여 세포내 유리 칼슘 농도를 상승시킬 것으로 추정된다. 중성호성 백혈구의 활성화증에 칼슘 유입과 유출은 동시에 이루어질 것으로 추정된다. In Ca⁺⁺ containing media, arachidonic acid markedly stimulated superoxide and H₂O₂ generation and activated NADPH oxidase. In Ca⁺⁺ free media, stimulatory action of arachidonic acid on NADPH oxidase was not detected. Arachidonic acid-stimulated respiratory burst was inhibited by EGTA, TMB-8, verapamil, diltiazem, nifedipine, dibucaine, lidocaine, CCCP, 2,4-dinitrophenol, sodium arsenate, chlorpromazine, theophylline, HgCl₂, PCMB and PCMBSA but not affected by tetrodotoxin, tetraethylammonium chloride and procaine. EGTA almost completely inhibited release of β-glucuronidase by arachidonic acid and verapamil, CCCP and theophylline slightly inhibited it, whereas dibucaine did not show any significant effect. Arachidonic acid induced Ca⁺⁺ release from intact neutrophils and it was decreased by TMB-8. Arachidonic acid-induced elevation of intracellular free Ca⁺⁺ level was inhibited by EGTA and CCCP and slightly inhibited by TMB-8. Amount of intracellular free Ca⁺⁺ increased by either arachidonic acid plus verapamil or arachidonic acid plus dibucaine was greater than that by arachidonic acid alone. These results suggest that various changes of biochemical events may be implicated in the functional expression in neutrophils activated by arachidonic acid. Arachidonic acid appears to elevate cytosolic free Ca⁺⁺ level by stimulating Ca⁺⁺ release from intracellular Ca⁺⁺ storage sites. During activation of neutrophils, Ca⁺⁺ influx and efflux may be accomplished, simultaneously.

탱크로리 위험요인 고찰

윤재건(Jae-Kun Yoon),장우정(Woo Jung Jang),손상근(Sang Geoun Son),이창수(Chang Soo Lee) 한국가스학회 1998 한국가스학회 학술대회논문집 Vol.1998 No.9

난분해성 환경오염물질의 고온ㆍ고압연소

윤재건(Jae Kun Yoon),홍호연(Ho Yeun Hong),이정우(Jeong Woo Lee),김종표(Jong Pyo Kim),강수석(Su Sok Kang) 한국연소학회 2006 KOSCOSYMPOSIUM논문집 Vol.- No.-

Disposal of highly toxic wastes like polychlorinated biphenyls (PCBs) is very difficult. These substances create a growing mountain of problematic waste that has to be disposed properly. Conventional technologies that are based on common burning(rotary kiln, ~1100℃) and plasma technology(~10000℃) do not satisfy important conditions, for example, complete combustion of the toxic waste and the price of waste disposal. The combustor like a rocket engine is operated at relatively high pressure(~15 bar) and relatively high temperature(>3000℃) that are ideal for the complete destruction of extremely toxic substances. In this study, test compound(o-DCB) was dissolved in kerosine with a concentration of 10%. Pure gas oxygen was used as an oxidant. Analysis showed that the destruction efficiency achieved for o-DCB was 99.9999% or better. The results show that a combustor based on liquid propllant rocket technology is a validated tool for the disposal of highly toxic waste, and a good alternative technology when applied to the destruction of extremely toxic wastes.

FLUX3D를 이용한 자기부상용 전자석의 특성 해석

이재건(Jae-Kun Lee),신판석(Pan-Seok Shin) 대한전기학회 1996 대한전기학회 학술대회 논문집 Vol.1996 No.7

6,6-Dichlorobicyclo[3, 1, 0]hexane-3-carboxylic acid의 합성과 분석

이광수,양재건,Lee, Kwang-Soo,Yang, Jae-Kun 한국분석과학회 2001 분석과학 Vol.14 No.1

6,6-Dichlorobicyclo[3, 1, 0]hexane-3carboxylic acid was synthesized by dichlorocarbene addition into 3-cyclopentenecarboxylic acid using BTEA.Cl as phase transfer catalyst. $^1H$ NMR $^{13}C$ NMR data analyst showed that this compound had boat-like conformation and carboxyl group existed as trans form. 6,6-Dichlorobicyclo[3, 1, 0]hexane-3carboxylic acid를 phase transfer catalyst(PTC)로 benzyl triethylamine chloride(BTEA.Cl)를 사용하여 3-cyclopentenecarboxylic acid로부터 합성하였다. $^1H$ NMR과 $^{13}C$ NMR 분석을 통하여 이 화합물은 boat-like conformation을 갖는 것으로 나타났고 carboxyl group은 trans로 존재하는 것으로 나타났다.

임플란트 시술용 지르코니아 소재의 연삭가공 성능 평가에 관한 연구

이상민(Sang-Min Lee),채승수(Seung-Su Chae),이충석(Choong-Seok Lee),김택수(Tack-Su Kim),이재건(Jae-Kun Lee),이종찬(Jong-Chan Lee) 한국기계가공학회 2014 한국기계가공학회지 Vol.13 No.1

3.2YSZ melted by the ‘skull melting’ method has good physical properties and does not undergo low-temperature degradation. Due to its excellent physical and mechanical properties, skull-melted 3.2YSZ has been developed as a dental implant material. In this study, a porous, resin-bonded diamond wheel was created and its grinding characteristics were compared with those of traditional nonporous wheels using skull-melted 3.2YSZ. The experimental results indicate that the porous, resin-bonded diamond wheel requires less grinding force and power. In addition, the porous, resin-bonded diamond wheel requires a greater degree of roughness.

3차원 유한요소 프로그램을 이용한 30 KVA 초전도발전기의 전자장 특성 해석

이종혁(Jong-Hyuk Lee),신판석(Pan-Seok Shin),이재건(Jae-Kun Lee),김용주,Do-Young Park 대한전기학회 1998 대한전기학회 학술대회 논문집 Vol.1998 No.7

6,6-Dichlorobicyclo[3, 1, 0]hexane-3-carboxylic acid의 합성과 분석

이광수(Kwang-Soo Lee),양재건(Jae-Kun Yang) 한국분석과학회 2001 분석과학 Vol.14 No.1

임플란트 시술용 드릴의 가공 성능 평가에 관한 연구

이상민(Sang-Min Lee),채승수(Seung-Su Chae),이재건(Jae-Kun Lee),최환(Hwan Choi),이종찬(Jong-Chan Lee) 한국기계가공학회 2014 한국기계가공학회지 Vol.13 No.2

Skull Melted 3.2YSZ has good physical properties and does not undergo low temperature degradation. Due to these excellent physical and mechanical properties, Skull Melted 3.2YSZ has been studied for use in dental implants. In this study, a ø2.2mm Initial Twist Drill was made usingSkull Melted 3.2YSZ; the drilling characteristics were compared with those of the traditional SUS420J drill. The experimental results indicate that the Skull Melted 3.2YSZ drill requires similar thrust forces and has a slightly higher temperature.