애플망고 미성숙과 추출물의 생리활성에 대한 연구

유단희 ( Dan Hee Yoo ),이인철 ( In Chul Lee ) 대한미용학회 2021 대한미용학회지 Vol.17 No.4

In the current study, we investigated the biological activities of ethanol extract of unripe Mangifera indica L. var. Irwin using a variety of in vitro and cell culture model systems for investigation of the anti-inflammatory and whitening effects. A total polyphenol, electron donating, 2, 2’-azinobis [3-ethylbenzothiazoline-6- sulfonic acid] (ABTS) radical scavenging assay was used for measurement of the antioxidant activities of unripe Mangifera indica L. var. Irwin. The content of phenolic compounds in unripe Mangifera indica L. var. Irwin was 121.47±0.47 mg/100 g. In measurement of electron donating and ABTS radical scavenging ability of unripe Mangifera indica L. var. Irwin, the effect was shown in a concentration-dependent manner. Inhibition of tyrosinase activity was 40.69% at 1,000 μg/ mL concentration of unripe Mangifera indica L. var. Irwin. An MTT assay was used to evaluate cell viability in order to evaluate the anti-inflammatory and whitening effects of unripe Mangifera indica L. var. Irwin in macrophages (RAW 264.7 cells) and melanoma (B16F10 cells). The result showed more than 95% at a concentration of 500 μg/ mL in RAW 264.7 cells and more than 95% at a concentration of 100 μg/mL in B16F10 cells. Nitric oxide inhibitory activity was confirmed for measurement of anti-inflammatory activity. Consequentially, unripe Mangifera indica L. var. Irwin showed an inhibitory effect of 62.64% at a concentration of 500 μg/mL. The amount of melanin production was measured in order to confirm the whitening effect; the inhibitory effect was 15.16% at a concentration of 100 μg/ mL. Thus, unripe Mangifera indica L. var. Irwin could be applied as an anti-inflammatory and whitening effect and could be used in the future as a safe, natural material for use in cosmetics.

십이지장 궤양 발생에 있어서 H . pylori와 위상피화생의 연관에 대한 연구

박선미(Seon Mee Park),양석균(Suk Kyun Yang),홍원선(Weon Seon Hong),민영일(Young Il Min),이인철(In Chul Lee) 대한소화기학회 1995 대한소화기학회지 Vol.27 No.6

N/A Background/Aims: H. pylori infection and gastric metaplasia are frequently associated in patients with duodenal ulcers. However, the pathogenesis of duwlenal ulcer in relation to H, pilori infecri<in and gastric metaplasia has not reacbed a consistent result, yet. The aim of this study v,as to assess whether H. pylori infection and gastric metapla.ia contrihute to duodenal ulcerogenesis. Methods: Multiple endoscopic biopsy specimens were tnken frorn duodenum and gastric antrum of ?I subjects(21 active duodenal ulcers; 30 scarred duodenal ulcers; 20 normal controls). The specimens were examined histologically for the presence ot' H. pylori, gastric metaplasia, and degree of gastritis and duodenitis. Rapid tissue urease test was performed t'or detection of H, pi krri infc cti<in. Results: The positive rate of H. pylrri in gastric antrurn was not significantly different aniong ttlTCC groups, while it was increasing in duodenum, I,e., normal controls(3%'), scarred DU's(37,r), active DU's(62%) in ascending order. The incidence of duodenal gastric metaplasia in the active DU'.I was 95.2% in contrast with the scarred duodenal ulcer's(72.07<) and the nonnal contrnls(2i.04) Diffuse gastric metaplasia lesions were more prevalent in active duodenal ulcers. 1n the duodenum, a strong association was shown between the H. pylrri and gastric rnetaplasia. The severity ot' duodenitis were significantly increased in the order ot' groups of normal controls, scarrecl duodena1 ulcers, and active duodenal ulcers. In duoclenum, there was a strong association with the grade c>f' intlammation anci H. pylori. Conclusions: These results suggest that gaslric metaplasia in the duodenum and concurrent H. pilori infection may play a synergistic role in the pathogene.I. Vt' duodenal ulcer. (Korean J Gastroenterol 199S;27: 617-62S)

제주도 자생 식물 혼합추출물의 in vitro 생리활성 평가

이인철 ( In-chul Lee ),박병권 ( Byoung-kwon Park ),김정미 ( Jeong-mi Kim ),문지영 ( Ji-young Moon ),김대용 ( Dae-yong Kim ) 대한미용학회(구 대한미용과학회) 2018 대한미용학회지 Vol.14 No.1

In this study moisturizing and anti-wrinkle effects of water extract from Jeju island plant mixture such as Portulaca oleracea, Opuntia ficusindica var. saboten, aloe vera, Ecklonia cava were investigated in vitro. Hyaluronic acid (HA) is a highly hydrophilic molecule known to be associated with skin moisture, wrinkle formation. Also, many studies have reported that collagen and elastin are a major factor in skin wrinkle formation. Therefore, we performed MTT assay, HA synthesis, hyaluronic acid synthase-2 (HAS-2) gene expression, collagen synthesis, hyaluronidase, collagenase and elastase inhibition assay for evaluation of biological activities. ELISA assay revealed that mixture increased HA and collagen production and RT-PCR also showed that mixture increased mRNA level of HAS-2 gene in HaCaT cells. And other results showed prevention of hyaluronidase, collagenase and elastase activation. These results indicate Jeju island plants mixture have moisturizing and anti-wrinkle effects. Therefore, Jeju island plants mixture can be used as a functional cosmetic.

미립화 및 분무장치 1 : 기체 공급 방식에 변화에 따른 2단 공기 보조식 분사기의 분무 분열 및 입도 분포 특성

이인철 ( In Chul Lee ),김도헌 ( Do Hun Kim ),구자예 ( Ja Ye Koo ) 한국액체미립화학회 2012 한국액체미립화학회 학술강연회 논문집 Vol.2012 No.-

Air-blast spray gun can produce homogeneous pattern of droplet distribution, so relatively fine film can be generated at the surfaces. In the spray painting and coating industries, air-blast injector configured with a liquid-centered and 2-stage gas outer nozzle have many advantages which are low paint bounce-back, high transfer efficiency. The experimental investigations are to study liquid column break-up and overspray characteristics. To verify the effect of gas jet, an air-blast injector with a gas post of 2-stages is designed to conduct overall spray characteristics as well as to increase transfer efficiency. Number of droplet distributions is measured by laser diffraction with Mie-scattering methods considering the ratio of refractive indices and absorption coefficient. The experiment results show that the main effect of liquid jet break-up is governed with gas jet of the inner-stage. In the case of inner gas injection, the exit at the inner stage transfer more shear force to center of liquid column. However, because the nozzle exit of outer stage is located to the far from liquid column, shear force to break-up liquid jet do not fully affect liquid column. For the outer-stage gas injection has less shear force to the liquid column than inner-stage gas injection. In case of inner stage gas jet, better atomization qualities can be achieved, however, in case of outer stage gas jet, larger droplet distributions are appeared on the same measurement area. As increase momentum ratio of gas jet, droplet sizes decreased. Additionally, gas jet of the outer-stage also contributes atomization at the far-field spray region and droplet transportation to the object. It was observed that, as the gas jets increase, droplet mean diameters decreased and transfer efficiency increased due to the outer gas post which makes an air-curtain around the spray jets so that impinged droplet cannot overspray to the outer region of the spray jets.

터빈 블레이드 회전수 변화와 터빈 블레이드 엣지 형상 변화에 따른 표면 가스온도 분포 해석

이인철(In Chul Lee),변용우(Yong Woo Byun),구자예(Ja Ye Koo),이상도(Sang Do Lee),김귀순(Kui Soon Kim),문인상(In Sang Moon),이수용(Soo Young Lee) 한국추진공학회 2008 한국추진공학회 학술대회논문집 Vol.2008 No.5

터빈 블레이드의 엣지 형상 변화에 따른 표면 가스온도 발달과정에 대해서 전산해석을 수행하였다. Sharp형과 Round형의 터빈 블레이드를 모델링하였으며, 터빈의 회전수는 두 가지 형상 모두 0 ~ 10,000 rpm의 회전 속도를 주어 전산해석을 실시하였다. 터빈의 회전수가 증가할수록 터빈 블레이드 표면의 평균 온도는 하강하였고, Sharp형 엣지 형상이 Round형 엣지 형상에 비해서 터빈 블레이드의 표면 온도 분포가 전반적으로 낮은 분포를 나타내었다. The numerical analysis for gas temperature of turbine blade surface has been performed to investigate development of temperature with various blade edge shape. Two different types of the turbine which one is "Sharp" edge and the other is "Round" edge was modeled. Computations have been carried out several turbine rotational speeds in the range from 0 to 10,000 rpm for the each types of turbine edge shape. As a result, the more rotational speed of turbine increased, the more turbine blade"s temperature decreased. It is also found that the surface temperature of turbine blades for sharp type edge were lower than the round type edge.

Do3 CuZnAl 합금에 있어서의 역형상기억효과

정인상,이인철,박정식,이순린 ( In Sang Chung,In Chul Lee,Jung Sig Park,Soon Lin Lee ) 한국열처리공학회 1990 熱處理工學會誌 Vol.3 No.2

Since the reverse shape memory effect(RSME) was reported in a CuZnAl alloy, further study has been done on the mechanism of this phenomenon and reported that it occurs by the bainitic transformation. But the present authors revealed in the previous work that the RSME in a B2 CuZnAl alloy is not caused by the shear process involved in the bainitic transformation and also that the RSME takes place as the remaining α`₂phase, which is two-step transformed strain induced martensite, is newly transformed into α phase. In order to provide further evidence in supporting the facts, thus, more detailed investigations have been carried out in a DO₃CuZnAl alloy.

아티초크 추출물의 항산화, 항염증, 미백 효능 활성에 관한 연구

유단희 ( Dan Hee Yoo ),이인철 ( In Chul Lee ) 대한미용학회(구 대한미용과학회) 2021 대한미용학회지 Vol.17 No.2

The objective of this study was to investigate the biological activities of 70% ethanol extracts from Cynara scolymus L. (CSEE). The total polyphenol content of CSEE were found to be 158.5 mg tannic acid/100 g dry weight of CSEE. To confirm the antioxidant effect, electron donating ability and ABTS radical scavenging effect were measured. As a result, the extent of electron donating and ABTS radical scavenging effect on CSEE increased in a dose-dependent manner. The tyrosinase inhibitory effect of CSEE, which is related to skin-whitening, was observed to be 36.25% at a concentration of 1,000 μg/mL. Furthermore, MTT assay was performed to confirm the cell viability in RAW264.7 and B16F10 cell lines. In both RAW 264.7 cell and B16F10 cells, the cell viability was observed more than 95% at 100 μg/mL in all extracts. The anti-inflammatory effect of CSEE on lipopolysaccharide (LPS)-induced RAW264.7 cells was also investigated. The CSEE significantly suppressed NO production in a concentration-dependent manner. In order to measure the whitening effect on CSEE, the expressions of proteins related to whitening, such as microphthalmia-associated transcription factors, tyrosinase-related proteins-1 were observed using western blot. In the section treated with the extracts, it was confirmed that the protein expression decreased in a concentration-dependent manner. In conclusion, CSEE was verified to have antioxidant, anti-inflammatory and whitening effects, and it was confirmed that it is a potential natural cosmetic material.

단삼사물탕의 육안적, 조직학적 육모촉진 효과

김정미 ( Jeong Mi Kim ),이인철 ( In Chul Lee ),이태종 ( Tae Jong Lee ),김배환 ( Bae Hwan Kim ) 대한미용학회(구 대한미용과학회) 2011 대한미용학회지 Vol.7 No.3

The purpose of this study was to evaluate the hair growth effect of Dansam-Samultang in C57BL/6 mice. In order to estimate its hair growth effect, we performed percutaneous application of sample on the back of six-week-old C57BL/6 male mice; 150 μL a day, six days a week, for four weeks. Experimental animals were classified into four groups - control group (C, saline), positive control group (PC, minoxidil 3%), experimental 1 group (E1, Dansam-Samultang ethanol 2%), and experimental 2 group (E2, Dansam-Samultang toner 2%). Body weight, water and feed consumption were measured once a week, and macroscopic hair growth effect was observed once a week. On the second and fourth week, we took a blood sample of five mice, analyzed their blood cells, observed their skin tissues. The experiment showed no significance in water and feed consumption, body weight gain, and feed efficiency among groups; relatively high weight gain, but no statistical significance for PC group. The organ weight had no significant difference among groups. In the hematological analysis, E1 had more white blood cells, neutrophils, and monocytes, but there was no significant difference in other items among groups. Macroscopic observation showed hair growth effect in the high to low order of PC, E1, E2, and C groups. Histological observation showed a clear increase of number and depth of follicle, and dermal thickness for PC, E1 and E2 groups, compared to C group. Taking all the results together, this experiment shows that Dansam-Samultang has a effective in hair growth and hair loss prevention.

대나무 숯과 대나무 잎의 급여가 육계의 생산성과 육질에 미치는 영향

Sung-Hwan Kim(김성환),In-Chul Lee(이인철),Sung-Su Kang(강성수),Changjong Moon(문창종),Sung-Ho Kim(김성호),Dong-Ho Shin(신동호),Hyoung-Chin Kim(김형진),Jin-Cheol Yoo(유진철),Jong-Choon Kim(김종춘) 한국생명과학회 2011 생명과학회지 Vol.21 No.6

본 연구는 대나무 숯과 대나무 잎의 급여가 육계의 성장과 사료효율 및 육질에 미치는 영향을 조사하기 위해 30일간 각각 0.5%의 농도로 급여하였다. 시험결과, 대숯과 대잎 시험군에서 시험기간 중 사망률은 대조군에 비해 낮아진 반면, 출하 시 체중과 증체량은 대조군에 비해 유의성 있게 증가하였다. 또한 대조군에 비해 대숯과 대잎 시험군의 사료효율도 개선되었으며, 계육의 전단력은 대조군에 비해 유의성 있게 감소하였다. 대숯을 급여한 닭에서의 지방함량은 통계학적 차이는 없었으나 감소경향을 나타낸 반면, 대숯과 대잎을 급여한 닭에서의 불포화 지방산 비율은 증가경향을 나타내었다. 계육의 관능검사 결과, 대숯과 대잎 시험군의 선호도는 대조군에 비해 높게 나타났다. 혈액 및 혈청생화학 검사항목에서는 대조군과 시험군간에 유의성 있는 차이가 인정되지 않았다. 본 시험결과는 대나무 숯과 대나무 잎의 사료첨가 급여가 육계의 성장과 사료효율 및 육질을 개선시키는 것으로 나타났다. 본 연구결과는 사료첨가제로서 대나무 숯과 대나무 잎의 유용성과 개발가능성을 시사해 주고 있다. The present study was conducted to investigate the effects of dietary supplementation with bamboo charcoal (BC) and bamboo leaf (BL) on growth performance, feed intake, feed conversion efficiency, and meat quality in broiler chickens. Broiler chickens were fed for 30 days with a diet containing 0.5% bamboo charcoal or bamboo leaf. The results showed that the mortality rate during the study period was slightly lower in the BC and BL groups than the control group, while terminal weight and weight gain were significantly higher in the BC and BL groups than the control group. Dietary supplementation with BC or BL also improved feed conversion rate compared to chickens in the control group. Shear force was significantly lower in the BC and BL groups than that of the control group. The fat content of chickens fed with BC tended to decrease, while the ratio of unsaturated fat acid of chickens fed with BC or BL tended to increase, although without a statistically significant difference. Sensory evaluation revealed that overall acceptability was slightly higher in the BC and BL groups than the control group. There was no statistically significant change in the hematology and serum biochemistry parameters, compared with the control group in any group tested. The results of this experiment indicated that dietary supplementation with BC and BL may improve growth performance, feed conversion efficiency, and meat quality in broiler chickens.

Effects of Dietary Supplementation of a New Probiotic CS61 Culture on Performance in Broiler Chickens

Sung-Hwan Kim(김성환),In-Chul Lee(이인철),Hyung-Seon Baek(백형선),Seong-Soo Kang(강성수),Hyoung-Chin Kim(김형진),Jin-Cheol Yoo(유진철),Jong-Choon Kim(김종춘) 한국생명과학회 2012 생명과학회지 Vol.22 No.3

가금류 제품에서 항생제의 잔류와 내성균의 출현으로 인해 항생제 대체물질에 대한 연구가 활발히 진행되고 있으며, 특히 생균제와 활성촉진제 또는 이들을 조합한 신바이오틱의 사용이 권장되고 있다. 본 연구는 새로운 생균제 CS61 배양액의 사료첨가 급여가 육계의 성장 및 사료효율에 미치는 영향과 안전성을 평가하여 항생제를 대체할 수 있는 사료첨가제로서의 개발가능성을 알아보기 위해 수행하였다. CS61 배양액은 0, 0.1 및 1%의 용량으로 28일간 사료에 혼합하여 육계에게 급여하였다. 시험결과, 시험물질 처치군에서 부검 시의 체중과 일당증체량이 대조군에 비해 용량의존적으로 증가하였다. CS61 배양액의 사료 내 첨가는 대조군 동물에 비해 사료효율도 개선하는 것으로 나타났다. 반면, 일반증상과 사망률, 부검소견, 혈액학치 및 혈청생화학치에서는 시험물질의 처치와 관련된 독성소견이 관찰되지 않았다. RAW 264.7 세포를 이용한 일산화질소 시험에서 정제된 CS61 펩타이드는 lipopolysaccharide에 유도된 일산화질소 생성을 용량의존적으로 억제하였다. 본 시험결과는 육계에 CS61 배양액의 사료첨가 급여는 항염증효과를 통해 성장과 사료효율을 개선할 수 있음을 보여주며, 사료첨가제로서 CS61 배양액의 유용성과 개발가능성을 시사해 주고 있다. Bacterial resistance to antibiotics and residues of antibiotics in poultry products have encouraged the use of probiotics, prebiotic substrates, and synbiotic combinations of prebiotics and probiotics as alternative approaches to the use of antibiotics in poultry. The present study was carried out to evaluate the effect of a new probiotic CS61 culture on growth performance, feed conversion efficiency, and safety in broiler chickens, and to evaluate its value as an alternative for antibiotics used as a feed additive. Two dosages of CS61 culture (0.1% and 1%) were fed to chickens for 28 days. The results showed that terminal body weight and daily weight gain in the treatment groups increased in a dose-dependent manner when compared with the control group. Dietary supplementation with CS61 culture also improved feed conversion rate compared to the control group. There were no treatment- related toxic effects in terms of clinical findings, mortality, necropsy findings, hematology, or serum biochemistry parameters in any group tested. The nitric oxide assay showed that CS61 peptide has a dose-dependent inhibitory effect on lipopolysaccharide-induced nitric oxide production in RAW 264.7 cells. The results of this experiment indicated that dietary supplementation of CS61 culture may improve growth performance and feed conversion efficiency in chickens through its anti-inflammatory effect.