지방조직 유래 줄기세포의 조골세포로의 분화에 대한 실험적 연구

이의석,장현석,권종진,임재석,Lee, Eui-Seok,Jang, Hyon-Seok,Kwon, Jong-Jin,Rim, Jae-Suk 대한악안면성형재건외과학회 2008 Maxillofacial Plastic Reconstructive Surgery Vol.30 No.2

Stem cells have self-renewal capacity, long-term viability, and multiline age potential. Adult bone marrow contains mesenchymal stem cells. Bone marrow-derived mesenchymal stem cells (BMSCs) are progenitors of skeletal tissue components and can differentiate into adipocytes, chondrocytes, osteoblasts, and myoblasts in vitro and undergo differentiation in vivo. However, the clinical use of BMSCs has presented problems, including pain, morbidity, and low cell number upon harvest. Recent studies have identified a putative stem cell population within the adipose tissue. Human adipose tissue contains pluripotent stem cells simillar to bone marrow-derived stem cells that can differentiate toward the osteogenic, adipogenic, myogenic, and chondrogenic lineages. Human adipose tissue-derived stem cells (ATSCs) could be proposed as an alternative source of adult bone marrow stem cells, and could be obtained in large quantities, under local anesthesia, with minimal discomfort. Human adipose tissue obtained by liposuction was processed to obtain ATSCs. In this study, we compared the osteogenic differentiation of ATSCs in a specific osteogenic induction medium with that in a non-osteogenic medium. ATSCs were incubated in an osteogenic medium for 28 days to induce osteogenesis respectively. Osteogenic differentiation was assessed by von Kossa and alkaline phosphatase staining. Expression of osteocyte specific bone sialoprotein, osteocalcin, collagen type I and alkaline phosphatase, bone morphogenic protein 2, bone morphogenic protein 6 was confirmed by RT-PCR. ATSCs incubated in the osteogenic medium were stained positively for von Kossa and alkaline phosphatase staining. Expression of osteocyte specific genes was also detected. Since this cell population can be easily identified through fluorescence microscopy, it may be an ideal source of ATSCs for further experiments on stem cell biology and tissue engineering. The present results show that ADSCs have an ability to differentiate into osteoblasts. In the present study, we extend this approach to characterize adipose tissue-derived stem cells.

고기능성 쌀단백질 소재 개발 연구

이의석(Eui-Suk Lee),김기종(Ki-Jong Kim),김재현(Jae-Hyeon Kim),홍순택(Soon-Taek Hong) 충남대학교 농업과학연구소 2010 농업과학연구 Vol.37 No.1

Rice bran proteins from different cultivars(Youngan, Sindongjin, Suwon 511) were extracted with Xylanase using orthogonal analysis method and their functional properties were investigated. The optimum extraction conditions, based on protein content in the extract found to be at 1 wt% xylanase, pH 7 and 50:1, solvent to rice bran ratio(v/w %). Nitrogen solubility indices(NSI) of rice bran protein concentrates were shown a minimum value at pH 4 ranged 2~23%, varied with different cultivars and a maximum (NSI≥90% for all cultivars) at pH 10. As for water adsorption and fat adsorption capacity, rice bran protein concentrates were shown to be better than Na-caseinate and isolated soy protein, respectively. Emulsifying activities were observed high in order of Na-caseinate>Youngan rice bran protein>Shindongjin rice bran protein>Suwon 511 rice bran protein>isolated soy protein. In general, the surface tension of rice bran protein solution(10?³ wt%, 5 mM bis-tris, pH 7) was increased with increasing concentrations and found a minimum value near pI. On heating, it was decreased slightly with increasing temperatures up to 70 ℃ and then increased above 80 ℃. Addition of sodium chloride was made the surface tension decrease. In conclusion, with Xylanase, rice bran protein concentrate can be successfully extracted from the rice bran of different cultivars and the Youngan rice bran protein was thought to have best functionality among rice cultivars tested. It might be used as a milk protein substitute.

초임계 CO2를 이용하여 추출한 탈지미강 중 표면활성물질의유화 성질 평가

이형주 ( Hyong Ju Lee ),배재석 ( Jae Suk Bae ),이의석 ( Eui Suk Lee ),강호철 ( Ho Cheol Kang ),이기택 ( Ki Teak Lee ),홍순택 ( Soon Taek Hong ) 한국산업식품공학회 2012 산업 식품공학 Vol.16 No.2

In this experiment, the surface-active substances were extracted from defatted rice bran and their emulsifying properties were investigated. The sample emulsions stabilized with the surface-active substances (3 fractions: 1-HS, 6- HS and 18-HS) were prepared and then their physico-chemical properties such as fat globule size, creaming stability, oil-off and dispersion stability were determined. It was found that the sample emulsion with different fraction of surface- active substances showed different physico-chemical properties from each other. Specially, 1-HS emulsion with the smallest particle size was evaluated to be superior to others in terms of higher creaming stability, low incidence of oil-off and higher dispersion stability. From the study of co-surfactant addition (Tween 20, SSL and GMS), GMS was found to be the most effective in reducing fat globule size in 1-HS emulsion. This indicated that there might be a co-operative adsorption of the two surface-active substances at the oil-water interface. Thus, the potentiality of the rice bran extracts, obtained with specific conditions, as natural surface-active substances was confirmed.

프랙탈 분석을 통한 악골 내 낭종의 감압술 후 골 치유에 대한 방사선학적 평가

백진우,석민,이의석,장현석,임재석,Baek, Jin-Woo,Seok, Min,Lee, Eui-Suk,Jang, Hyun-Seok,Rim, Jae-Suk 대한구강악안면외과학회 2007 대한구강악안면외과학회지 Vol.33 No.5

Purpose: This study was done to know the usefulness of fractal analysis when evaluating the radiologic changes after decompression on jaw bone cystic lesions using fractal analysis. Materials and methods: 30cases of cystic lesions were followed up after decompression. Panoramic image was used to observe radiologic changes around the cystic lesion. The part of the panoramic image which showed radiologic change was defined as region of interest(ROI); The fractal dimension of the ROI was calculated using box-counting method. Results: Using sign-rank test, there was a statistically significant difference in fractal dimensions after decompression therapy(P<0.0001). The fractal dimensions statistically increased after decompression(the median of D:0.12). Conclusions: The ROI after decompression showed higher fractal dimensions which offer the objective proof of the bone healing around cystic lesions after decompression treatment.

오디 분말을 첨가한 식빵의 품질 특성

배재석(Jae-Seok Bae),이의석(Eui-Suk Lee),홍순택(Soon-Taek Hong) 충남대학교 농업과학연구소 2010 농업과학연구 Vol.37 No.2

This study was carried out to investigate the quality characteristics of bread containing mulberry fruit powder(0, 3, 6 and 9%, w/w). For color values, L, a and b values decreased with increasing the amount of mulberry fruit powder. In texture, increasing the amount of mulberry fruit powder resulted in hardness and chewiness to increase, while springiness and cohesiveness was decreased. Dough volume of bread containing mulberry fruit powder was reduced and specific loaf volume was also decreased with increasing the amount of mulberry powder. With addition of 6% mulberry powder to the bread, overall sensory score showed the highest rating, while it was minimum with 9% addition. In conclusion, it could be expected that a range of mulberry fruit breads can be developed, with good sensory properties.

압출성형공정을 이용한 고아미 후레이크 제조

김철(Jin Tie),이의석(Eui-Suk Lee),홍순택(Soon-Teak Hong),류기형(Gi-Hyung Ryu) 한국식품과학회 2007 한국식품과학회지 Vol.39 No.2

고식이섬유 쌀인 고아미 2호의 식품소재로서의 활용을 모색하고자 압출성형공정으로 고아미 후레이크를 제조한 다음 후레이크의 효소저항전분(RS) 함량, 페이스트 점도, 수분용해지수와 수분흡착지수, 체적밀도, 색도, 압착강도, 조직감 유지시간 등 품질 특성을 조사하였다. 압출성형 공정조건은 배럴온도 90, 110, 130℃ 그리고, 수분함량 50, 55%이었다. 압출성형 펠릿과 탈지한 후레이크의 RS함량은 각각 8.00~8.56%, 6.57~9.53% 범위로 수분함량이 50%에서 55%로 증가함에 따라 증가하는 경향을 나타내었다. 압출생형 펠릿과 탈지한 후레이크의 최고점도, 최저점도, 최종점도, 회복점도는 1시간 수침한 고아미에 비해 감소하는 경향을 나타내었고 구조파괴점도는 현저히 증가하는 경향을 나타내었다. 탈지한 후레이크의 수분용해지수와 수분흡착지수가 고아미 원료와 입출성형 펠릿보다 크게 증가하는 경향을 나타내었다. 압출성형 펠릿의 수분용해지수는 고아미 원료에 비해 감소하는 경향을 나타내었으며 수분흡착지수는 증가하는 경향을 나타내었다. 후레이크의 체적밀도는 0.35~0.44 g/㎖ 범위로 큰 차이를 나타내지 않았고 조직감 유지시간은 12.4~19.4부 범위로 시판 breakfast cereal들 보다 긴 유지시간을 나타내었다. Resistant starch (RS) content, paste viscosity, water solubility and absorption index, bulk density, color, compression force, and bowl life of Goami flakes manufactured by extrusion process were determined to evaluate Goami (high fiber rice) as a food material. Various extrusion process conditions included barrel temperature (90, 110, 130℃) and moisture content (50, 55%). RS content in the extruded pellet and defatted flake ranged from 8.00~8.56% and 6.57~9.53% respectively. RS content increased as moisture increased from 50% to 55%. Peak viscosity, trough viscosity, final viscosity, and setback viscosity of the extruded pellet and defatted flake decreased with steeping for 1 hr, and the breakdown viscosity significantly increased. The water solubility and absorption index increased in the flake compared to Goami and extruded pellet. The water solubility index of the extruded pellet was lower than that of Goami, while its water absorption index was higher than Goami. The bulk density of the flake ranged from 0.35~0.44 g/㎖. The bowl life of the flake was 12.4~19.4 min, which was longer than commercial breakfast cereals on the market.

양극산화 처리된 타이타늄 표면에서 골형성 유전자 발현

김원석,김영석,전성배,전상호,이의석,장현석,권종진,임재석,Kim, Won-Seok,Kim, Young-Seok,Jeon, Seong-Bae,Jun, Sang-Ho,Lee, Eui-Suk,Jang, Hyon-Seok,Kwon, Jong-Jin,Rim, Jae-Suk 대한악안면성형재건외과학회 2012 Maxillofacial Plastic Reconstructive Surgery Vol.34 No.2

Purpose: The purpose of this study was to evaluate the expression of osteogenic genes associated with bone regeneration on anodizing titanium surface. Methods: $20{\times}20{\times}1$ (mm) commercially pure titanium plate was made, one group was pure titanium, second group was punched, and last group was punched and anodized by electrochemical method. Through the osteogenic cell culture model, the expression of extracellular matrix proteins, such as bone morphogenetic protein-2, bone sialoprotein, aggrecan, osteocalcin, Alkaline phosphatase, collagen I had been evaluated by Real-time polymerase chain reaction, and the morphology of growing cells was evaluated by scanning electron microscopy. Results: The attachment of mesenchymal stem cell was even and well-oriented on all Ti surfaces. The osteogene expression was increased on punching groups but, decreased on anodizing surfaces in 3 week samples. Conclusion: Punched anodizing Ti has possibility be using as a dental implant material, but further in vivo study would be needed.

백서 두개골 결손부의 골재생에 fibrin glue가 미치는 영향에 관한 실험적 연구

임재석,장현석,윤정주,이의석,박은용,서제덕,Rim, Jae-Suk,Jang, Hyon-Seok,Eune, Jung-Ju,Lee, Eui-Seok,Park, Eun-Yong,Suh, Je-Duck 대한악안면성형재건외과학회 2005 Maxillofacial Plastic Reconstructive Surgery Vol.27 No.5

Fibrin glue is composed of fibrinogen and thrombin and used in various regions for multiple use. Basic principle is that thrombin converts fibrinogen to fibrin in the presence of $Ca^{2+}$. The structure of fibrin is loose at the beginning, but after about 5 minutes a tight structure is formed under the influence of factor VIII which changes fibrin monomer into fibrin polymer. Fibrin glue is used for tissue adhesive, suture, local hemostasis, wound healing, closure of subdural space. Fibrin adhesive has been used in oral and maxillofacial surgery for hemostasis after tooth extraction in patients with coagulation disorders, skin graft fixation, reattachment of periodontal flaps, in combination with autogenous bone chips to fill the bony cavities following cyst removal, and for securing the hydroxyapatite granules for maxillary alveolar ridge augmentation. This study was designed for researching influence of fibrin glue during healing phase after making artificial bone defect.

HA/TCP 골이식재상에 이식된 지방유래 줄기세포의 골모세포로의 분화 및 골형성에 대한 연구

임재석,권종진,장현석,이의석,정유민,이태형,박정균,Rim, Jae-Suk,Gwon, Jong-Jin,Jang, Hyon-Seok,Lee, Eui-Seok,Jeong, You-Min,Lee, Tai-Hyung,Park, Jeong-Kyun 대한악안면성형재건외과학회 2010 Maxillofacial Plastic Reconstructive Surgery Vol.32 No.2

Aim of the study: An alternative source of adult stem cells that could be obtained in large quantities, under local anesthesia, with minimal discomfort would be advantageous. Adipose tissue could be processed to obtain a fibroblast-like population of cells or adipose tissue-derived stromal cells (ATSCs). This study was performed to confirm the availability of ATSCs in bone tissue engineering. Materials amp; Methods: In this study, adipose tissue-derived mesenchymal stem cell was extracted from the liposuctioned abdominal fat of 24-old human and cultivated, and the stem cell surface markers of CD 105 and SCF-R were confirmed by immunofluorescent staining. The proliferation of bone marrow mesenchymal stem cell and ATSCs were compared, and evaluated the osteogenic differentiation of ATSCs in a specific osteogenic induction medium. Osteogenic differentiation was assessed by von Kossa and alkaline phosphatase staining. Expression of osteocyte specific BMP-2, ALP, Cbfa-1, Osteopontin and osteocalcin were confirmed by RT-PCR. With differentiation of ATSCs, calcium concentration was assayed, and osteocalcin was evaluated by ELISA (Enzyme-linked immunosorbant assay). The bone formation by 5-week implantation of HA/TCP block loaded with bone marrow mesenchymal stem cells and ATSCs in the subcutaneous pocket of nude mouse was evaluated by histologic analysis. Results: ATSCs incubated in the osteogenic medium were stained positively for von Kossa and alkaline phosphatase staining. Expression of osteocyte specific genes was also detected. ATSCs could be easily identified through fluorescence microscopy, and bone formation in vivo was confirmed by using ATSC-loaded HA/TCP scaffold. Conclusions: The present results show that ATSCs have an ability to differentiate into osteoblasts and formed bone in vitro and in vivo. So ATSCs may be an ideal source for further experiments on stem cell biology and bone tissue engineering.

찰보리 전분 추출에 있어서 β -Glucanase 처리 효과

배재석(Jae-Seok Bae),이의석(Eui-Suk Lee),정용선(Yong-Sun Jeong),김정원(Jeong-Won Kim),이미자(Mi-Ja Lee),홍순택(Soon-Taek Hong) 충남대학교 농업과학연구소 2012 농업과학연구 Vol.39 No.3

In the present study, β-glucanase-assisted extraction of starch from glutinous barley(Hinchal ssalbory) was investigated. β-glucanase was added to a coarse starch suspension obtained after wet milling in the starch extraction process. It was found that in the isolated starch with enzyme treatment, protein content was lower by 0.03%, compared to that with non-enzyme treatment. More importantly it was observed that the extraction yield of starch from enzyme treatment was found to be about 12% higher than the one from non-enzyme treatment (enzyme treated: 90.56%, non-enzyme treated: 78.46%). In order to elucidate this finding, the mass-balance determination of starch in each extraction step was carried out and found that the enzyme treatment might influence on the insoluble residues(R3 and R4 fractions) to hydrolyze β-glucan and other materials (e.g., mucilages etc.), thereby facilitated the separation of starch from it and a next filtration process. With a phase-contrast microscope it was observed that the isolated starch with enzyme treatment contained small starch granules more than the one with non-enzyme treatment and this might result in higher extraction yield observed with the former. In order to confirm this hypothesis, further experiments would be necessary.