Rat에서 ethylene glycol에 unfolded protein response의 조절

이은령(Eun Ryeong Lee),권기상(Kisang Kwon) 한국생명과학회 2013 생명과학회지 Vol.23 No.9

에틸렌 글리콜(ethylene glycol)은 자동차 부동액 주성분으로 우리 실생활에 널리 쓰이고 있다. 접근이 용이하고 달콤한 맛 때문에 자살목적이나 보관 및 사용 시 의도적으로 또는 실수로 인한 오용사고가 자주 발생한다. 에틸렌 글리콜은 그 자체로는 인체의 독성이 낮지만 생체에서 대사과정을 거치면서 독성이 높아진 유기산을 만들어 다양한 조직에서 광범위한 세포손상을 유발한다. 다양한 세포 스트레스가 소포체(ER) 샤페론과 소포체 스트레스 센서의 유전자 발현을 유도하는 것은 이미 알려져 있다. 본 연구에서는 rat 조직에서 소포체 샤페론과 소포체 스트레스 센서 유전자의 발현 조절이 에틸렌 글리콜에 의해 유도되고, 조직학적 변화도 H&E 염색 및 면역 형광 염색에 의해 확인하였다. Ethylene glycol (EG) is the most commonly used for automotive antifreeze, and it"s easily misuseful for human. EG poisoning occurs in suicide attempts and infrequently, either intentionally through misuse or accidentally because of sweet taste. Though EG itself is mild toxic to the human body, it becomes higher toxic organic acids by in vivo broken down that are responsible for extensive cellular damage in various tissues caused principally by the metabolites. It is already well known that various cellular stresses induce gene expression of endoplasmic reticulum (ER) chaperones and ER stress sensors. This study demonstrated that regulation of gene expression of ER chaperones and ER stress sensors was induced by EG in rat tissues, and in tissues histological changes are also detected by both staining H&E and immunofluorescent.

노인 우울에 미치는 요인

이은령(Eun-Ryeong Lee),강지혁(Ji-Hyuk Kang),정재필(Jae-Pil Jung) 한국콘텐츠학회 2013 한국콘텐츠학회논문지 Vol.13 No.7

본 연구의 목적은 노인들의 우울에 영향을 주는 요인으로 신체적 건강상태, 경제상태, 가족지지, 사회적 지지, 자아존중감에 종교생활 참여로 인해 영향 주는지를 규명하여 노인우울증 예방 프로그램 개발을 위한 기초 자료와 우울을 해소하는 방안을 제공하고자 한다. 연구대상은 경북 구미, 김천, 칠곡 인근지역에 있는 노인회관과 노인복지관, 가정방문을 통하여 357명의 노인들을 대상으로 직접면접방식으로 설문하였다. 자료 분석은 SPSS/PC 18.0과 AMOS 18.0 프로그램을 이용하여 분석한 결과, 첫째, 일반적 특성에 따른 우울요인에는 성별, 학력, 배우자 유·무, 동거상태, 주관적 건강상태에서 유의한 차이를 보였다. 둘째, 종교생활에 영향을 미치는 요인으로는 경제상태, 가족지지, 자아 존중감 이었다. 셋째, 우울에 정적으로 가장 영향을 많이 미치는 요인은 신체적 건강상태로 나타났다. 넷째, 종교생활 참여는 개인적 참여 수준이 높을수록 우울이 감소하는 것으로 나타났다. 본 연구에서 도출된 결론을 통해 노인들의 우울을 관리하는데 있어 신체적 건강상태에 더하여 가족지지, 사회적지지, 자아존중감과 함께 평가하여 우울증을 조기 발견하고 관리하는 통합적인 프로그램의 개발과 시행이 절실하다. 특히 노인들의 종교생활 참여가 노인 우울을 감소시켜 건강한 노년기를 보내는데 보탬이 될 것으로 사료된다. The purpose of this research is to examine the influence to physical health status, economic status, family support, social support and self-esteem which affects to solve depression, and give basic information for developing a program to prevent the aged people gloominess and a way to solve that gloominess. This surveying study was undertaken through personal interview way for 357 aged people who were in the elder’s meeting hall, living in elderly welfare institude, or home near Gumi, Gimcheon, and Chilgok regions. For data analysis, SPSS/PC 18.0 and AMOS 18.0 program for frequency analysis, First, In the reason of depression by general distinctive quality, there was a significant difference in sex`educational background`existance of a spouse of the condition of living-together, and personal health condition. Second, affecting factors to religious life were economic condition, family support, and self-esteem. Third, the most serious factor that enhanced depression was physical health. Fourth, It appeared as higher attendence of indivisuals about religion means decrease of the depression. When these result reviewed, to control the depression of aged people, it is necessary to develop and conduct a total program that can detect and manage depression early through measuring the physical health, family support, social support and self-esteem. Especially, participation of religious life is considered to ameliorate the depression of aged people and help them live a good senescence.

쌍별 귀뚜라미의 소화기관에서 기아에 의한 소화효소 유전자의 발현

이누리(Nuri Lee),이은령(Eun-Ryeong Lee),권기상(Kisang Kwon),권오유(O-Yu Kwon) 한국생명과학회 2020 생명과학회지 Vol.30 No.1

쌍별 귀뚜라미(G. bimaculatus)가 식량공급이 제한적이거나 starvation상태를 어떻게 극복하는지를 알기 위하여 소화기관(전장, 중장, 후장)에서 소화작용의 가장 중요한 3대 효소인 amylase, trypsin, lipase의 유전자발현을 조사하였다. G. bimaculatus의 전장에서는 amylase, trypsin, lipase 유전자, 중장은 amylase 유전자, 후장은 amylase, trypsin 유전자의 발현이 먹이 의존적이지만, 중장의 trypsin, lipase 유전자와 후장의 lipase 유전자는 먹이 비의존적인 발현으로 항상 일정한 상태를 유지한다. 이 결과는 곤충이 starvation과 같은 외부환경에 적응 및 생존하는지를 관련 유전자들의 발현수준에서 접근할 수 있는 중요한 실마리를 제공할 것이다. The gene expression of amylase, trypsin, and lipase in the digestive organs of the two-spotted cricket (Gryllus bimaculatus) was tested to understand how it overcomes starvation. Amylase gene expression in the foregut was reduced by digesting no food until starvation-3 days. Although that expression persisted to starvation-6 days, it returned to normal at refeeding-2 days. The expression of trypsin peaked at around 8 times as starvation started and at around 4 times at starvation-3 days. After refeeding, trypsin expression rose up to 14 times and then fell back to normal as feeding continued. Lipase gene expression remained elevated at 1.5-2 times when starvation started and returned to normal at refeeding-2 days. In the midgut, amylase expression decreased until starvation-3 days, increasing to about 2 times at starvation-6 days; it did not rise again by refeeding. Trypsin was constantly expressed regardless of starvation and refeeding, while lipase expression was reduced by 0.6-0.7 times by starvation and refeeding. Amylase gene expression in the hindgut was 0.2-0.3 times lower than starvation-6 days, and it increased by 0.5 times on refeeding-1 day and more than 1.5 times on refeeding-3 days. The gene expression of trypsin was almost identical to amylase.

천연물에 의한 초파리수명연장 효과

이정훈(Jeong Hoon Lee),권기상(Kisang Kwon),이은령(Eun Ryeong Lee),유보경(Bo-Kyung Yoo),고영화(Young Hwa Ko),최지영(Ji-Young Choi),권오유(O-Yu Kwon) 한국생명과학회 2017 생명과학회지 Vol.27 No.1

우리는 이전실험에서 배양세포를 사용하여 4종류의 천연물(Corydaline, (±)-Car-3-ene-2,5-dione, Cinobufagin, Corilagin)이 ERAP1와 FOXO1 (DFA16) 유전자발현을 2배 이상 상승시키는 것을 증명하였다. 본 실험은 1% agar, 5% sucrose, natural compound 20 μl를 넣은 먹이를 만들어 4시간 starvation후에 4시간 동안 먹였다. Cinobufagin와 Corilagin를 먹이면 대조군에 비하여 6-8일 정도 더 생존하였다. RT-PCR 실험결과 ERAP1와 FOXO1 유전자 발현을 조절하는 것이 증명되었다. 산업곤충질병 진단과 치료에 사용될 것이며, 초파리를 대신한 생쥐에서도 동일한 결과를 실험하여 수명연장을 위한 신약으로 발전시킬 것이다. A previous study demonstrated that four kinds of natural compounds, Corydaline, (±)-Car-3-ene-2,5-dione, cinobufagin, and corilagin, enhanced the gene expression of ERAP1 and FOXO1 (DFA16) more than two-fold in a cell culture system. In this study, the experimental food was made finally 30 ml in which included 1% agar, 5% sucrose, and each natural compound 20 μl. A fruit fly of Drosophila melanogaster fed a natural product for 4 hr after 4 hr starvation. Both natural compounds of Cinobufagin and Corilagin induced 6-8 days more survival comparing than it controls group. The resulting fruit flies were estimated the gene expression of ERAP1 and FOXO1 by RT-PCR that also demonstrated meaningful results with the same lifespan results. Cinobufagin from BufonisVenennumis has C26H34O₆ molecular formula and 442 kDa molecular weight. Corilagin from Euphorbiapekinensisis has C27H22O18 molecular formula and 634 kDa molecular weight. The two types of natural products screened in this study will be used in the early diagnosis and treatment of insect industry in the near future. In addition, the natural products will be used in longevity experiments in a mouse model. The results may give one of the clues for studying new drug development candidates of the longevity.

Isolation and Characterization of Six Microorganisms from the Digestive Tract of the Cricket Gryllus bimaculatus

Kisang Kwon(권기상),Eun Ryeong Lee(이은령),Bo-Kyung Yoo(유보경),Young Hwa Ko(고영화),Hyojung Shin(신효정),Ji-Young Choi(최지영),O-Yu Kwon(권오유) 한국생명과학회 2017 생명과학회지 Vol.27 No.9

우리는 처음으로 쌍별귀뚜라미 소화기관에서 6종류의 미생물을 분리하고 특성을 규명하였다. 이들은 16S rDNA을 기준으로 분류한 결과 4종류(Staphylococcus, Bacillus, Citrobacter, Proteus)에 속하였다. 분리된 6종류의 미생물은 공통적으로 ampicillin에 저항성을 보이지만 kanamycin 저항성은 보이지 않았다. 이들을 Gram염색하여 미생물의 형태적 특징을 확인하였다. Gram-positive한 rod-shaped GL2와 round-shaped GL4는 다른 분리 균 보다 많은 양의 세포외분비물을 만들었다, 이들을 MALDI-TOF-MS spectral analysis결과 87-kDa collagenase, 56-kDa & 200-kDa hypothetical protein이였다. 새롭게 분리된 6종류의 미생물은 귀뚜라미의 생리에 미치는 영향과 이들의 생물공학적 혹은 해충 방제에 이용될 수 있는 연구가 기대된다. We describe the isolation and characterization of six different intestinal microorganisms from the digestive tract of the cricket Gryllus bimaculatus. Based on 16S rRNA gene sequences, we obtained six isolates belonging to four different genera: Staphylococcus, Bacillus, Citrobacter, and Proteus. All the isolates were resistant to ampicillin. Ampicillin is an irreversible inhibitor of the enzymeetranspeptidase, which is needed to make bacterial cell walls. None of the isolates were resistant to kanamycin, which binds to the 30S subunit of the bacterial ribosome and then inhibits total protein synthesis. Gram staining was conducted, in addition to morphological classification under a microscope. Four grampositive isolates and two gram-negative isolates were detected. The gram-positive isolates were GL1 (round shaped, 2 am in diameter), GL2 (rod shaped, 2.5 μm in length), GL3 (rod shaped, 2 μm in length), and GL4 (round shaped, 1.5 μm in diameter). The gram-negative isolates were GL5 (rod shaped, 2 μm in length) and GL6 (rod-shaped, 2.5 μm in length). Notably, two of the isolates, GL2 and GL4, secreted specific extracellular proteins. These were determined by MALDI-TOF-MS spectral analysis to be a 87 kDa collagenase, 56 kDa hypothetical protein, and 200 kDa hypothetical protein. The six isolates in this study could be used for various biotechnological applications and pest management, both in the field and in greenhouse systems. In addition, it would be interesting to determine the relationship between these isolates and their host.

노인들의 건강지각이 건강증진행위에 미치는 영향

이소윤 ( Soo Yum Lee ),이은령 ( Eun Ryeong Lee ),여미영 ( Mi Young Yeo ) 한국노인의료복지학회 2010 노인의료복지연구 Vol.2 No.1

This study aimed to analyze the primary factors which have effect on health promoting behavior of elderly and prompt the method to form the right health habit of elderly, so that it can be applied as the basic data for development of health promoting behavior program. The data was analyzed by SPSS 12.0 program, the internal consistency reliability analysis and t-test, ANOVA, and the result of study is as follows. 1) The health promoting behavior of general characteristic by the health perception In the health promoting behavior by sex distinction, the group with high health perception had high health promoting behavior, and in the health promoting behavior by sex distinction and health perception, there was relevance between sex distinction and health perception but there was no interaction effect between sex distinctions. In health promoting behavior by age, the group with high health perception had high health promoting behavior, and in the health promoting behavior by age and health perception, there was no relevance with age but health perception, and there was no interaction effect between the sex distinction and health perception. In the health promoting behavior by level of education and health perception, there was no relevance with level of education but health perception, and here was on interaction effect between the level of education and health perception. In the health promoting behavior by living arrangement and health perception, there was no relevance with living arrangements but health perception, and there were no interaction effects between living arrangements and health perception. In the health promoting behavior by monthly income and health perception, there was no relevance with monthly income but health perception, and there was no interaction effects between monthly income and health perception. In the health promoting behavior by current health status and health relevance with current health status but health perception, and there was no perception, there interaction effects between current health status and health perception. 2) The primary factor affect on health promoting behavior In the results of regression analysis of health promoting behavior adding health perception on general characteristic and life style, these independent variables were expressing 51.4% of the health promoting behavior, so it increased 13.2 % more than 38.2% of descriptivity of general characteristic and life style, and the value of F was 35.189, so there was statistical significance. The characteristics which had significant effect on health promoting behavior were sex distinction(b=-0.175), life style(b=0.352) and health perception standard(b=0.442).

노인의 건강상태가 삶의 질에 미치는 영향

정재필 ( Jae Pil Jung ),이은령 ( Eun Ryeong Lee ),신미숙 ( Mi Sook Sin ) 한국노인의료복지학회 2012 노인의료복지연구 Vol.4 No.1

The purpose of this study is to determine the relationship between the health-promoting behaviors and quality of life and adjusting effects in state of health targeting the elderly who live in Korea. According to the analysis of the secondary materials from <2009 The Fourth National Health and Nutrition Examination Survey> by Korea Centers for Disease Control and Prevention on 1575 seniors in the age older than 65 years, the following conclusions were obtained. 1) The difference in health status by the general characteristics. The health status appeared to be better in male subjects, lower age, higher level of education, living with spouse, living in the residential town houses, with more economic activity and higher house hold income and with company medical insurance. 2) The quality of life by the general characteristics and health status. The variable that regulate the health status was economic activity. In both group, with and without economic activity, subjects with higher health status had better quality of life and there was a correlation found between the economic activity and health status. 3) The relationship between factors related to quality of life. In the relationship between factors related to the quality of life, it was found that the health status(r=0.493) were significantly correlated displaying better quality of life with more higher health status. 4) Factors affecting the quality of life. Factors affecting quality of life are seen as the following attributes, gender, age, housing type, economic activity, total household income, health insurance type, and health status.It was appeared that the quality of life is better in male subjects, lower age, residential apartments or townhouse housing type, economic activity, higher household income, local medical insurance, and higher health status.

혈청 요산 농도와 대사증후군과의 연관성에 대한 연구

박덕현(Duk-Hyun Park),이은령(Eun-Ryeong Lee),주정열(Joung-Youl Ju) 한국콘텐츠학회 2017 한국콘텐츠학회논문지 Vol.17 No.2

본 연구는 사회적으로 관심이 증가하고 있는 심혈관질환의 위험인자인 대사증후군과 혈청 요산 농도와의 연관성을 알아보기 위함이다. 연구 대상자는 건강검진센터에서 검진한 수진자 총 1,444명을 대상으로 시행하였으며, 남성은 977명, 여성은 467명을 대상으로 하였다. 대상자들은 신체계측과 혈액검사를 시행하여 혈청 요산과 대사증후군 위험인자들과의 상관성과 혈청 요산의 등급을 구분하여 발생하는 위험인자의 수를 비교하였다. 연구결과 평균연령은 남녀 각각 41.63±6.29세, 35.75±6.61세로 나타났다. 혈청 요산의 평균수치는 남성이 6.30±1.39 mg/dL, 여성은 4.43±0.89 mg/dL로 남성이 높게 나왔다. 혈청 요산 농도에 따른 상관관계는 남성에서는 나이, 허리둘레, 수축기 혈압, 중성지방, HDL-cholesterol, WBC count, ESR이 상관관계가 있었으며(p<0.05), 여성의 경우는 허리둘레, 이완기 혈압, 중성지방, HDL-cholesterol, ESR이 상관관계가 있다고 나왔다(p<0.05). 혈청 요산 농도의 등급에 따른 위험인자 수는 혈청 요산 농도가 증가할수록 증가하였다. 대사증후군의 전체적인 유병률은 남성이 17.4%로 여성의 10.2% 보다 높게 나왔다. 대사증후군과 혈청 요산 농도와의 통계적으로 유의한 관련성이 있다는 것을 알 수 있었고, 혈청 요산의 농도가 증가할수록 대사증후군의 위험인자 또한 관련성이 커진다는 것을 알 수 있었다. 혈청 요산 농도와 대사증후군과의 연관성연구를 통해 대사증후군을 진단받지 않은 젊은 성인에서 대사증후군의 예방을 위한 대상자 선정에 혈청요산 농도를 활용할 수 있다. This research aimed to identify the correlation between the metabolic syndrome, which is one of the cardiovascular risk factors and emerging as an issue in the society, and the serum uric acid level. The research was conducted on a total of 1,444 patients who took a medical examination at a health examination center. The research subjects were composed of 977 men and 467 women. By conducting physical measurement and the blood test on the subjects, the research identified the correlation between the serum uric acid level and the metabolic syndrome and compared the number of the risk factors by dividing the class of the serum uric acid. The average level of serum uric acid was identified higher in men at 6.30±1.39 mg/dL than 4.43±0.89 mg/dL in women. Men had a correlation between age, waist size, systolic blood pressure, neutral fat, HDL-cholesterol, WBC count and ESR and the serum uric acid level (p<0.05), while women had a correlation between waist size, diastolic blood pressure level, neutral fat, HDL-cholesterol and ESR and the serum uric acid level (p<0.05). The number of the risk factors depending on the serum uric acid level increased as the serum uric acid level rose. The overall prevalence rate of the metabolic syndrome was higher in men at 17.4% than 10.2% in women. This showed that there is a statistically relevant relevance between the metabolic syndrome and the serum uric acid level, and the number of the risk factors of the metabolic syndrome will go up with an increase in the serum uric acid level.

B-type Natriuretic Peptide 수치와 이완기 심초음파 파라미터와의 연관성 연구

배성조 ( Seong-jo Bae ),권기상 ( Kisang Kwon ),이은령 ( Eun Ryeong Lee ) 대한임상검사과학회 2016 대한임상검사과학회지(KJCLS) Vol.48 No.4

The b-type natriuretic peptide (BNP) values and increase on functional disorder in the ventricle, and are used as an index to diagnose heart failure and predict the prognosis. BNP values is known to be relevant to dyssystole in congestive heart failure. This study aimed to identify correlation between the BNP values and the items that indicate the diastolic function in echocardiography. The research divided 188 patients who went through the BNP test and echocardiography in the hospital into the groups with the BNP values; <100, 100-300, 301-600, 601-900, and >901 pg/mL. As the BNP values increase, there was relevance with the echocardiography items of ejection fraction, size of left atrium, E velocity, A velocity, Deceleration time, E/A ratio, E`, A`, S` and E/E`. In comparison on the groups divided based on the BNP values, E/E` had the highest relevance. The research also categorized 67 patients who diagnosed with heart failure. In comparison on the groups of the heart failure patients, the BNP values of the three groups of Grade I: 623.0±459.7 pg/mL, Grade II: 1013.2±1155.1 pg/mL and Grade III: 1693.4±1544.0 pg/mL, respectively (p< 0.01). As the grade was higher, there was a higher relevance with the echocardiography items of ejection fraction, size of left atrium, E velocity, A velocity, Deceleration time, E/A ratio, E`, A`, S` and E/E` (p<0.001). Higher BNP values had a higher relevance with the items that indicate the diastolic function in echocardiography and the BNP values of the Restrictive physiology group were the highest in echocardiography. So the BNP values was thought to be valuable to predict diastolic function of heart.

장수풍뎅이(Allomyrina dichotoma)에 Oryctes rhinoceros nudivirus 감염에 의해서 유전자 발현이 조절되는 5개의 유전자

유보경(Bo-Kyung Yoo),권기상(Kisang Kwon),고영화(Young Hwa Ko),이은령(Eun Ryeong Lee),최지영(Ji-Young Choi),권오유(O-Yu Kwon) 한국생명과학회 2016 생명과학회지 Vol.26 No.11

최근에 장수풍뎅이(Allomyrina dichotoma)는 관상용으로 인기가 높으며, 애벌레는 건강보조식품으로 주목을 받고 있다. 장수풍뎅이의 대량사육 시 발생하는 질병의 원인이 Oryctes rhinoceros nudivirus (OrNV)인 것이 2015년에 처음 보고되었다. 그러나 아직 정확한 진단, 발병기전과 치료방법을 찾지 못하고 있으며 곤충 사육농가에 매년 심각한 경제적 손실을 입고 있다. 본 연구는 장수풍뎅이의 대량사육 시 발생하는 OrNV질병의 조기진단과 치료에 실마리를 제공하기 위하여 OrNV에 감염된 장수풍뎅이의 장(intestine)에서 유전자발현이 조절되는 Klf15, ERAP2, Snrnp200, mbnl2a, MIMI_L93를 보고한다. Korean rhinoceros beetles (Allomyrina dichotoma), which can be found in broad-leaved forests in mountainous habitats and lives for around one year in wild. This beetle is currently popular as a pet and traditionally regarded as a medicine for liver-related diseases in Korea. It is reported that the economic losses in the mass-rearing facilities by virus infection have been increased since the 2010s in Korea. The causing virus for the A. dichotoma was firstly reported as an Oryctes rhinoceros nudivirus (OrNV) in 2015. We, here, observes that serious morphological changes in the intestinal tube from the OrNV-infected beetles, and report five genes, which are regulated by OrNV infection in the intestine; Krueppel-like factor 15 (Klf15), Endoplasmic reticulum aminopeptidase 2 (ERAP2), U5 small nuclear ribonucleoprotein 200 kDa helicase (Snrnp200), Muscleblind-like protein 2a (mbnl2a), and MIMI_L93. The results may provide a clue to the early diagnosis and disease treatment during the mass-rearing facilities of the A. dichotoma.