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피하 주머니 이식술을 이용한 수부 무지 첨부 재건술 - 2예 보고 -
이영근,문영재,이준모,Lee, Young-Keun,Moon, Young-Jae,Lee, Jun-Mo 대한미세수술학회 2011 Archives of reconstructive microsurgery Vol.20 No.2
Purpose: The subcutaneous pocket graft of the thumb tip amputation across or proximal to the lunula is chosen in case of impossible microvascular anastomosis and in patient who strongly desired to preserve the thumb tip after failed replantation. Materials and Methods: Two patients who underwent a subcutaneous pocket graft for a thumb tip reconstruction between August 2008 and November 2009 were reviewed retrospectively. They were all males with a mean age at the time of surgery of 48 years and had sustained complete thumb tip amputations across or proximal to the lunula. In one case, the microsurgical replantation was not feasible and the other one revealed arterial insufficiency at the 7th day after microsurgical replantation. Results: Authors had experienced 2 cases of flaps which survived completely. The results of sensibility was good, the range of motion at interphalangeal joint and tip to tip pinch was acceptable and color mismatch and loss of thumb finger nail was unacceptable after more than 1 year follow up with conventional successful thumb tip replantation. Conclusion: The subcutaneous pocket graft could be chosen in thumb tip amputation in case of impossible microvascular anastomosis as well as who strongly desires to preserve thumb tip after failed replantation.
돼지여포의 퇴화과정 중 여포액 내 스테로이드 호르몬 양의 변화
이영근,윤용달,Lee, Young-Keun,Yoon, Yong-Dal 대한생식의학회 1985 Clinical and Experimental Reproductive Medicine Vol.12 No.1
In order to study the mechanism of follicular atresia, the follicles of the porcine ovary were isolated according to the presence or absence of the corpus luteum and their size, and then classified to the normal? or atretic?follicle on the morphological observation such as the transparency, the vascularization of follicle, the nuclear phase of oocyte, and the homogeneity of the granulosa cell layer. The viability of granulosa cells was examined. The concentrations of progesterone ($P_4$), testosterone (T), and estradiol-17 beta ($E_2$) in each follicular fluid were estimated by the radioimmunoassay. The viability of granulosa cells in the atretic follicle was much lower than that of the normal one. The concentration of each steroid hormone increased as the follicular size was increased, was not different in quantity between the normal- and the atretic follicle of which diameter was below 3mm, and were much higher in the atretic follicle than those in the normal one of which diameter was above 7mm. The ratio of the concentration of E2 to T in the large atretic follicle valued higher than that in the normal one, but smaller in the small and medium atretic follicle than that in the normal one. The present study suggests that the mechanism of atresia of the large follicle may be different from that of the small and the medium follicle and that the amount of steroid hormones regarded as the one of the criteria for the atretic follicles.
방사선을 이용한 느타리 ( Pleurotus ostreatus ) 의 섬유소 분해 변이주 선발
이영근,장화형,김원록,이창주,김진규 ( Young Keun Lee,Hwa Hyoung Chang,Won Rok Kim,Chang Joo Lee,Jin Kyu Kim ) 한국환경생물학회 1998 환경생물 : 환경생물학회지 Vol.16 No.2
To isolate the cellulolytic variants, the basidiospores of Pleurotus ostreatus were irradiated at the dose of 1Gy∼20kGy of gamma-ray. After irradiation the germination and survival rates of basidiospores were observed and the activity of extracellular enzyme was determined by means of MUF-substrate method. The 45 variants showed various levels of mycelial growth rate and cellulolytic activity on solid media and vari ous levels of activities of the extracellular enzymes. We have isolated highly cellulolytic variants. 1.5KG-2 and 2KG-2. among the above variants. It is suggested that the isolated 2 variants could be applied usefully to reuse and/or recycle of the cellulosic bioresources.
이영근,박찬일,이준모,Lee, Young-Keun,Park, Chan-Il,Lee, Jun-Mo 대한미세수술학회 2012 Archives of reconstructive microsurgery Vol.21 No.1
Purpose: To report clinical appearances and the outcomes of patients treated for wrist laceration with suicidal intent. Materials and Methods: Between March 2005 and February 2008, patients with wrist laceration were treated at our center. Among them, 14 patients with suicidal intent were reviewed retrospectively. They were 8 females and 6 males with a mean age of 33 years at the time of treatment. The side of injured wrist, injured structures, suicidal instrument, the time of injury, a suicidal motive, drug or alcohol abuse or not, indication of combined psychiatric disease, the duration of postoperative treatment, and the results of treatment were analyzed. Results: The injured wrist was the left one in 13 cases (93%), and knife wounds in 11 cases (79%) was the most frequent mechanism of injury. An average of 4.6 structures were injured including 3.5 tendons, 0.57 nerves and 0.5 arteries. The most frequently injured structures were the palmaris longus (71%), FCU (50%), FCR (43%), FDS 3 (36%), FDS 2, median nerve, and ulnar artery (each 29%). Simultaneous injury of both median and ulnar nerves occurred in 3 cases (21%), and simultaneous injury of both radial and ulnar artery occurred in 1 case (7%). The injury time was the night in 12 cases (86%), and the most frequent suicidal motive was human relations such as lover or spouse in 12 cases (86%). In 9 cases (64%), the injured were in a drunken state, 3 cases (21%) had been treated for a psychiatric disease at the time of the injury. The durations of postoperative treatment were an average of 5 days of admission and 1 day follow up. Only 1 case results could be evaluated. Conclusions: Patient's poor compliance in wrist laceration with suicidal intent should be expected and these characteristics may affect the management plan.
수지 재건시 불충분한 혈행상태의 수혜부에 시행한 동맥화 정맥 유리 피판술
이영근,박기태,이준모,박혁,Lee, Young-Keun,Park, Ki-Tae,Lee, Jun-Mo,Park, Hyuk 대한미세수술학회 2012 Archives of reconstructive microsurgery Vol.21 No.2
Purpose: Arterialized venous flap is useful for reconstruction of the traumatic soft tissue defect in fingers, but insufficient circulation of the traumatic fingers makes surgeons annoying to use the flap. We have grafted flaps in 7 fingers with insufficient vascular bed hoping to expanded the category of the flap. Materials and Methods: Arterialized venous flap have transplanted in 7 fingers from March 2008 through February 2010 and followed up for 4 to 16 months(average 7.2 months). They were all male with a mean age at the time of surgery was 33. The main injury was crushing in 4 degloving, contact burn and saw injury was I respectively. Time interval from injury to flap transplantation was average 3.1. weeks(3 days to 6 weeks). Designed flap size ranges from $8cm{\times}3.5cm$to $4cm{\times}3cm$. Vessel type of flap was one artery with two veins were 5 cases and one artey with one vein 2. Flap type was cutaneous in 3, tendocutaneous 2, neurotendocutaneous 1 and neurocutaneous 1. The circulation state of recipient site was avascular in 2 cases, insufficiency 3 and tip avascular 2. Results: Arterialized venous flap was complete survived in 2 cases, partial necrosis(less than 10%) 3 and failed in 2. Conclusion: An arterialized venous free flap could be a useful procedure for reconstruction in soft tissue or combined defect of the finger despite an avascular or insufficient vascular beds if the recipient beds were free from infection.
유리 동맥화 정맥 피판을 이용한 수지 연부조직 결손의 재건
이영근,우상현,이준모,안희찬,천호준,Lee, Young-Keun,Woo, Sang-Hyun,Lee, Jun-Mo,Ahn, Hee-Chan,Cheon, Ho-Jun 대한미세수술학회 2010 Archives of reconstructive microsurgery Vol.19 No.1
Purpose: To report the clinical results of the use of arterialized venous free flaps in reconstruction in soft tissue defects of the finger and to extend indications for the use of such flaps based on the clinical experiences of the authors. Materials and Methods: Eighteen patients who underwent arterialized venous free flaps for finger reconstruction, between May 2007 and July 2009 were reviewed retrospectively. The mean flap size was 4.7${\times}3.2$ cm. The donor site was the ipsilateral volar aspect of the distal forearm in all cases. There were 8 cases of venous skin flaps, 5 cases of neurocutaneous flaps, 4 cases of tendocutaneous flaps, 1 case of innervated tendocutaneous flap. The vascuality of recipient beds was good except in 4 cases (partial devascuality in 2, more than 50% avascuality (bone cement) in 2). Results: All flaps were survived. The mean number of included veins was 2.27 per flap. Mean static two-point discrimination was 10.5 mm in neurocutaneous flaps. In 3 of 5 cases where tendocutaneous flaps were used, active ROM at the PIP joint was 60 degrees, 30 degrees at the DIP joint and 40 degrees at the IP joint of thumb. There were no specific complications except partial necrosis in 3 cases. Conclusions: An arterialized venous free flap is a useful procedure for single-stage reconstruction in soft tissue or combined defect of the finger; we consider that this technique could be applied to fingers despite avascular recipient beds if the periphery of recipient bed vascularity is good.
이영근(Young Keun Lee),장화형(Hwa Hyoung Chang),한갑진(Gab Jin Han),김진규(Jin Kyu Kim),김재성(Jae Sung Kim) 한국환경농학회 1998 한국환경농학회지 Vol.17 No.4
In order to determine the optimal radiation dose for the sterilization of biowastes, the bioburden, frequencies and radiosensitivities of bacteria, mold and fungi in rice straw, chaff, corn stover and sawdust was observed before and after γ-ray irradiation. Radiation sterilization dose of rice straw, chaff, com stover and sawdust was calculated as 17.7, 17.6, 15.6 and 20.0kGy, respectively, from the mutual compensation of screening dose and derived dose on the basis of 10^(-3) SAL. This method could be acceptable for the sterilization of various biowastes including food, pharmaceuticals, etc.